FastQCFastQC Report
Sat 18 Jun 2016
SRR1930959_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930959_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3716167
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67740.182284595929085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57960.15596715648139603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41100.057.5453951
GTATCAA76100.041.064291
TCAACGC97400.031.6808094
CAACGCA97850.031.381545
ATCAACG98250.031.2634533
TATCAAC101550.030.8105872
AACGCAG105150.029.3834326
GTGGTAT19800.025.9048841
ACGCAGA124700.024.510397
GTACATG104350.024.0761071
CGCAGAG129800.023.3645258
TACATGG109450.022.730552
ACATGGG106200.022.6199283
TGGTATC22000.020.717772
GCAGAGT148700.019.9161059
AGAGTAC137450.018.68027910-11
CATGGGG75850.018.4006674
GAGTACA82250.018.2462771
CAGAGTA143250.017.70858610-11
GAGTACT95550.017.31023212-13
Produced by FastQC (version 0.11.5)