##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930959_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3716167 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.238212114794624 31.0 31.0 33.0 30.0 34.0 2 31.352521294118375 31.0 31.0 34.0 30.0 34.0 3 31.43575894194206 31.0 31.0 34.0 28.0 34.0 4 35.30413272600505 37.0 35.0 37.0 33.0 37.0 5 35.08805147884904 35.0 35.0 37.0 32.0 37.0 6 35.13163321239331 37.0 35.0 37.0 32.0 37.0 7 35.00952594433996 36.0 35.0 37.0 32.0 37.0 8 34.985703010655875 36.0 35.0 37.0 32.0 37.0 9 36.493266852646826 38.0 35.0 39.0 32.0 39.0 10-11 36.415936232144574 38.0 35.0 39.0 32.0 39.0 12-13 36.3114192661417 38.0 35.0 39.0 32.0 39.0 14-15 37.325779492686955 39.0 36.0 41.0 32.0 41.0 16-17 37.290604674117176 39.0 36.0 40.5 32.0 41.0 18-19 37.2794426353821 39.0 36.0 40.0 31.5 41.0 20-21 37.239415101635636 39.0 36.0 40.0 31.0 41.0 22-23 37.11151853509274 39.0 36.0 40.0 31.0 41.0 24-25 36.98435242549648 39.0 36.0 40.0 31.0 41.0 26-27 36.70454664712323 38.5 35.5 40.0 30.0 41.0 28-29 36.518646632403765 38.0 35.0 40.0 30.0 41.0 30-31 36.27605540870472 38.0 35.0 40.0 30.0 41.0 32-33 36.02795716661818 38.0 34.5 40.0 29.5 41.0 34-35 36.21825795234714 38.0 35.0 40.0 30.0 41.0 36-37 36.266226463988296 38.0 35.0 40.0 30.0 41.0 38-39 36.225347784424116 38.0 35.0 40.0 30.0 41.0 40-41 36.1236342446397 38.0 35.0 40.0 30.0 41.0 42-43 35.97166919570622 38.0 35.0 40.0 29.0 41.0 44-45 35.77641290071195 38.0 34.5 40.0 28.5 41.0 46-47 35.544123555265415 38.0 34.0 40.0 27.5 41.0 48-49 35.330795279114206 38.0 34.0 40.0 27.0 41.0 50-51 35.04619181538397 38.0 33.5 40.0 26.5 41.0 52-53 34.82491152846468 38.0 33.0 40.0 26.0 41.0 54-55 34.63547036502935 38.0 33.0 40.0 26.0 41.0 56-57 34.37150954195546 37.0 33.0 40.0 25.5 41.0 58-59 34.11753158563649 37.0 33.0 40.0 25.0 41.0 60-61 33.79652098519792 36.5 32.0 39.5 24.0 41.0 62-63 33.47947858640367 36.0 32.0 39.0 23.0 41.0 64-65 33.1630349228116 36.0 31.5 39.0 23.0 40.5 66-67 32.81168607331156 35.0 31.0 39.0 22.0 40.0 68-69 32.432167876201476 35.0 31.0 38.0 22.0 40.0 70-71 32.021670985184464 35.0 31.0 38.0 21.0 40.0 72-73 31.59830962924971 34.5 30.0 37.0 20.0 39.5 74-75 31.07732927502989 34.0 30.0 36.5 18.5 39.0 76-77 29.724536464588375 32.5 28.5 35.0 17.5 37.5 78-79 30.486746559021704 34.0 29.5 36.0 18.0 38.0 80-81 30.49145207952172 34.0 30.0 35.0 18.0 37.0 82-83 30.216053126783592 34.0 30.0 35.0 18.0 37.0 84-85 29.898717549561148 34.0 29.0 35.0 16.0 36.5 86-87 29.559452387365802 34.0 29.0 35.0 11.0 36.0 88-89 29.206091383944802 34.0 29.0 35.0 7.0 36.0 90-91 28.866552552670534 34.0 29.0 35.0 4.5 35.0 92-93 28.506187961951117 33.0 29.0 35.0 2.0 35.0 94-95 28.086082649138213 33.0 27.5 35.0 2.0 35.0 96-97 27.61034555228546 33.0 27.0 35.0 2.0 35.0 98-99 27.1063816561527 33.0 26.5 35.0 2.0 35.0 100-101 25.876306150934553 32.0 23.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.49490942159197004 1101 2 0.5489804396763276 1101 3 0.5350173153039108 1101 4 0.30944528809035887 1101 5 0.388281248877675 1101 6 0.3879402951436006 1101 7 0.4879729563629027 1101 8 0.4127940357159616 1101 9 0.5829266990281994 1101 10-11 0.5090165111035532 1101 12-13 0.5208069761197649 1101 14-15 0.7579091490908283 1101 16-17 0.7811945629493025 1101 18-19 0.78206479701349 1101 20-21 0.6673807963125213 1101 22-23 0.7054028666757759 1101 24-25 0.5873954209521841 1101 26-27 0.7478983193564943 1101 28-29 0.6482489573447054 1101 30-31 0.7462886933082942 1101 32-33 0.6671149242807388 1101 34-35 0.6163201191995427 1101 36-37 0.7198235116041118 1101 38-39 0.8053731385098004 1101 40-41 0.7920808654908456 1101 42-43 0.7772036067615531 1101 44-45 0.7751882047251755 1101 46-47 0.8777921592293438 1101 48-49 0.883846653105671 1101 50-51 0.7781056628869081 1101 52-53 0.6685142046610295 1101 54-55 0.8338121988970997 1101 56-57 0.8453701317259359 1101 58-59 0.7556215726752669 1101 60-61 0.7478692033782934 1101 62-63 0.7250914036265925 1101 64-65 0.827648830992679 1101 66-67 0.929318037494788 1101 68-69 0.7041839614798562 1101 70-71 0.6708214827176988 1101 72-73 0.77122362357591 1101 74-75 0.7076710216868669 1101 76-77 0.8887026753715936 1101 78-79 0.9139181538515011 1101 80-81 0.7165121735598632 1101 82-83 0.9995282425233647 1101 84-85 1.0318002739086474 1101 86-87 0.9288109203269279 1101 88-89 0.8627276154877457 1101 90-91 0.8162989595846533 1101 92-93 1.0426128630891434 1101 94-95 0.9322482944644825 1101 96-97 0.8860282606915497 1101 98-99 0.990352867238407 1101 100-101 1.1648500052056328 1102 1 0.5243309768680007 1102 2 0.621749245195339 1102 3 0.5411407796179404 1102 4 0.3509639401671336 1102 5 0.43313548236692867 1102 6 0.43516302683415375 1102 7 0.5207776059874902 1102 8 0.47525051227793114 1102 9 0.696815351234946 1102 10-11 0.6715208977462126 1102 12-13 0.6635570652967786 1102 14-15 0.766918915959728 1102 16-17 0.7584436650414546 1102 18-19 0.7359667427331971 1102 20-21 0.6439759095564952 1102 22-23 0.8017738315060896 1102 24-25 0.8540103264389884 1102 26-27 0.8341325292481372 1102 28-29 0.8634307484813064 1102 30-31 0.968478786933332 1102 32-33 0.9540213194641751 1102 34-35 1.0387362964155713 1102 36-37 0.9618585960803756 1102 38-39 1.1302707474104636 1102 40-41 1.0361361162479454 1102 42-43 1.151279198543115 1102 44-45 1.1818712457841514 1102 46-47 1.0814520994699066 1102 48-49 1.0274315402371812 1102 50-51 1.072139973292245 1102 52-53 1.1408751426732593 1102 54-55 1.0478142900439522 1102 56-57 0.9397736060913431 1102 58-59 1.0188074955598978 1102 60-61 0.9641903301713981 1102 62-63 0.829173255851245 1102 64-65 0.9228547901835427 1102 66-67 0.8797890148893188 1102 68-69 0.8914352586985359 1102 70-71 1.0000151853040435 1102 72-73 0.8962752162701797 1102 74-75 1.0575761041268699 1102 76-77 1.2762840358590246 1102 78-79 1.219816220551575 1102 80-81 1.1341806675730872 1102 82-83 1.3728465878600922 1102 84-85 1.3468505018696497 1102 86-87 1.3006154948550837 1102 88-89 1.3826527573405762 1102 90-91 1.3126030958095853 1102 92-93 1.4363933980934682 1102 94-95 1.3363944480106973 1102 96-97 1.4244033688231923 1102 98-99 1.3511089872367315 1102 100-101 1.7393525996430093 1103 1 0.36883726127889105 1103 2 0.5189397728503806 1103 3 0.4169016675221222 1103 4 0.2766020494724799 1103 5 0.22461325617319972 1103 6 0.3499611153299469 1103 7 0.5061311387804182 1103 8 0.37344560490831213 1103 9 0.5514270691973806 1103 10-11 0.5039260330406776 1103 12-13 0.4367805710750403 1103 14-15 0.5945402559449064 1103 16-17 0.5633797646816774 1103 18-19 0.5167117756079591 1103 20-21 0.6203662384673834 1103 22-23 0.6497384924509717 1103 24-25 0.6260008705997109 1103 26-27 0.6104440548039207 1103 28-29 0.593465882201258 1103 30-31 0.6903907178641049 1103 32-33 0.7670993175336278 1103 34-35 0.6243288446925703 1103 36-37 0.6505533038129485 1103 38-39 0.6503779258884066 1103 40-41 0.7156410894994565 1103 42-43 0.763715305929189 1103 44-45 0.8723130276314564 1103 46-47 0.8086861615599901 1103 48-49 0.789169303852745 1103 50-51 0.7512668791560699 1103 52-53 0.6355833611217108 1103 54-55 0.8514128240241021 1103 56-57 0.9211927805087754 1103 58-59 0.8954407058329608 1103 60-61 0.8590949901529896 1103 62-63 0.7570696998912183 1103 64-65 0.6956814273089904 1103 66-67 0.6156410628752127 1103 68-69 0.6969354667095473 1103 70-71 0.7041622444340945 1103 72-73 0.6254748907759229 1103 74-75 0.7852404476049948 1103 76-77 0.8062622658248344 1103 78-79 0.9165253020273845 1103 80-81 0.8094755305604515 1103 82-83 0.8291543841462037 1103 84-85 0.9577053313161237 1103 86-87 1.0318535574600602 1103 88-89 0.9330920098191733 1103 90-91 0.9387358192214279 1103 92-93 0.916028001490993 1103 94-95 0.9671260172079421 1103 96-97 0.8422396924731004 1103 98-99 0.9770109198377845 1103 100-101 1.2156208963723216 1104 1 0.38928926078587267 1104 2 0.4574935697234004 1104 3 0.4662610680063324 1104 4 0.25332980739636923 1104 5 0.35365664691039456 1104 6 0.40640516235515634 1104 7 0.3597580160656264 1104 8 0.3170424606529849 1104 9 0.4762523532548286 1104 10-11 0.4668543640813425 1104 12-13 0.485710424190394 1104 14-15 0.5644880895491653 1104 16-17 0.5417405264291872 1104 18-19 0.6602271330957734 1104 20-21 0.5680097711692795 1104 22-23 0.6128315271326343 1104 24-25 0.5585851164779356 1104 26-27 0.7285665766952647 1104 28-29 0.5833693168389402 1104 30-31 0.6671563740138495 1104 32-33 0.7765502473546491 1104 34-35 0.6230627366662418 1104 36-37 0.6876580910417189 1104 38-39 0.730569069516882 1104 40-41 0.690046369029595 1104 42-43 0.6411153647226442 1104 44-45 0.6752598024753667 1104 46-47 0.6282057281408555 1104 48-49 0.6073182862170938 1104 50-51 0.6450411566400476 1104 52-53 0.6469905905326456 1104 54-55 0.5951760222519695 1104 56-57 0.6281772186778198 1104 58-59 0.6268243973728218 1104 60-61 0.6470816281262515 1104 62-63 0.5778815276885112 1104 64-65 0.551754515573954 1104 66-67 0.6738399343275034 1104 68-69 0.7131543368166149 1104 70-71 0.6182844890879764 1104 72-73 0.64795663048886 1104 74-75 0.6549166089722256 1104 76-77 0.8397380418592917 1104 78-79 0.8699483824566379 1104 80-81 0.8254562905530385 1104 82-83 1.016668113717433 1104 84-85 1.0515195897212486 1104 86-87 0.933773222098317 1104 88-89 1.0502694318999097 1104 90-91 0.9865454554087023 1104 92-93 0.9985317563174689 1104 94-95 1.0730145479415292 1104 96-97 1.0473628497572278 1104 98-99 1.0615621689940404 1104 100-101 1.4386769137058089 1105 1 0.1679793395840008 1105 2 0.3105101701622104 1105 3 0.21998143595160968 1105 4 0.1673870833460569 1105 5 0.19457221996781726 1105 6 0.230942748041997 1105 7 0.24214793901533227 1105 8 0.23187103401098597 1105 9 0.4563505629179616 1105 10-11 0.2528349578413156 1105 12-13 0.3394534014116246 1105 14-15 0.5041974262863533 1105 16-17 0.2728416544433756 1105 18-19 0.3696660258172386 1105 20-21 0.2632645045519624 1105 22-23 0.42968278656530146 1105 24-25 0.43348799258969706 1105 26-27 0.4135543484865707 1105 28-29 0.40153582041868674 1105 30-31 0.5217646608596809 1105 32-33 0.5534041086747123 1105 34-35 0.539052261586761 1105 36-37 0.5101540226961632 1105 38-39 0.4368574605971034 1105 40-41 0.45890925817064954 1105 42-43 0.3864426265440244 1105 44-45 0.4342080772187842 1105 46-47 0.45490354242303965 1105 48-49 0.5353103243092576 1105 50-51 0.5953655980949364 1105 52-53 0.5710584461669512 1105 54-55 0.6743594334653764 1105 56-57 0.5411420329507237 1105 58-59 0.5674982200022143 1105 60-61 0.5636454882413702 1105 62-63 0.45518082153759565 1105 64-65 0.4822914709817141 1105 66-67 0.4554776933410949 1105 68-69 0.5490291006274788 1105 70-71 0.5134748373436437 1105 72-73 0.5338667150866065 1105 74-75 0.7854559477882823 1105 76-77 0.7507828883346939 1105 78-79 0.7600333708242815 1105 80-81 0.7194513926489812 1105 82-83 0.7491637011017893 1105 84-85 0.7300614999700379 1105 86-87 0.6636123685203579 1105 88-89 0.7345687297213424 1105 90-91 0.5516917834371853 1105 92-93 0.5028248705184772 1105 94-95 0.4075412295353935 1105 96-97 0.2763368189662394 1105 98-99 0.5695120604827544 1105 100-101 1.0148780521805065 1106 1 0.1243143114860672 1106 2 0.16257563100055705 1106 3 0.16770376987602376 1106 4 0.05592805259712463 1106 5 0.152959741300279 1106 6 0.06729507745782826 1106 7 0.1648601468556805 1106 8 0.07466657249478459 1106 9 0.1473883798071185 1106 10-11 0.21811719705429766 1106 12-13 0.10527586330475458 1106 14-15 0.15645403323082263 1106 16-17 0.16252109229733236 1106 18-19 0.2093508521015366 1106 20-21 0.20860759918738125 1106 22-23 0.30270807001756594 1106 24-25 0.17311981962680534 1106 26-27 0.25497752925356565 1106 28-29 0.2234006910807409 1106 30-31 0.23630111007671672 1106 32-33 0.22465232945145885 1106 34-35 0.1657123927208488 1106 36-37 0.27860562209682627 1106 38-39 0.1856527429352397 1106 40-41 0.0786023050650968 1106 42-43 0.2119090251254292 1106 44-45 -0.02302392516358509 1106 46-47 0.032880246702859495 1106 48-49 0.11884401006849288 1106 50-51 -0.04198627213292383 1106 52-53 -0.23501376817074515 1106 54-55 -0.1648397833119546 1106 56-57 0.12507243213189412 1106 58-59 0.11623904535355933 1106 60-61 -0.08598861911698208 1106 62-63 -0.04073945988945127 1106 64-65 -0.08493505446470095 1106 66-67 -0.03298182470936695 1106 68-69 -0.11032383479154362 1106 70-71 -0.20438070274366993 1106 72-73 -0.04193340174080973 1106 74-75 0.19112457650304648 1106 76-77 0.2075894129689324 1106 78-79 0.1595654364100696 1106 80-81 0.17548882948460331 1106 82-83 0.26579403707953375 1106 84-85 0.3336897357614994 1106 86-87 0.32640530910393295 1106 88-89 0.3375880989157629 1106 90-91 0.3401240469631901 1106 92-93 0.37077318531942893 1106 94-95 0.29544782174627215 1106 96-97 0.2475428173564751 1106 98-99 0.2035815436411781 1106 100-101 0.6421162031002865 1107 1 0.1198545213428126 1107 2 0.1402985382190245 1107 3 0.11252148498638803 1107 4 1.6923253472356237E-4 1107 5 0.0979492340624688 1107 6 0.07398489124879859 1107 7 0.05767089211353493 1107 8 0.07356570404046892 1107 9 0.11441967111218787 1107 10-11 0.11519075389058742 1107 12-13 0.1306383964388118 1107 14-15 0.06798558528819143 1107 16-17 0.16046528924709236 1107 18-19 0.04769470981615598 1107 20-21 0.16409314363510674 1107 22-23 0.23591909320548865 1107 24-25 0.16047603298905244 1107 26-27 0.12537231955625572 1107 28-29 0.1633268575487108 1107 30-31 0.15602013018293093 1107 32-33 0.29511189638662927 1107 34-35 0.17531496917990808 1107 36-37 0.2299728744875651 1107 38-39 0.1374454535968681 1107 40-41 0.036959339257080615 1107 42-43 0.17700576063001705 1107 44-45 0.08975093193696182 1107 46-47 -0.036954697603121645 1107 48-49 0.1310911394787837 1107 50-51 -0.0808566232073531 1107 52-53 -0.10334799810799211 1107 54-55 -0.08068673456721598 1107 56-57 -0.1129204900116818 1107 58-59 0.06921658465412861 1107 60-61 -0.16353414276624534 1107 62-63 -0.2418406883568167 1107 64-65 -0.2116340588743455 1107 66-67 -0.2475672131171507 1107 68-69 -0.16132263954823145 1107 70-71 -0.18905700473084153 1107 72-73 -0.207056469627954 1107 74-75 0.02033413186636679 1107 76-77 0.16593114660659936 1107 78-79 0.17040247187752655 1107 80-81 0.14621539752141288 1107 82-83 0.16265737201406694 1107 84-85 0.14498375635630367 1107 86-87 0.1114646649685973 1107 88-89 0.13260824421702821 1107 90-91 0.24449545390444527 1107 92-93 0.24247238002168103 1107 94-95 0.24487627532567657 1107 96-97 0.12531658927484557 1107 98-99 0.06491712002720007 1107 100-101 0.431991504868364 1108 1 0.2415512040788137 1108 2 0.3134453105696764 1108 3 0.3208751639702605 1108 4 0.138521233561967 1108 5 0.1656288860501718 1108 6 0.19480122226871543 1108 7 0.20772996590704906 1108 8 0.24284626990064595 1108 9 0.26818367827726775 1108 10-11 0.27559762128622367 1108 12-13 0.30186197725455344 1108 14-15 0.4162450697718256 1108 16-17 0.4352750355661428 1108 18-19 0.3579537520456668 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-0.5341313883694312 2316 74-75 -0.3834527620620847 2316 76-77 -0.8730281470339385 2316 78-79 -0.7152585760206627 2316 80-81 -0.6945044161492824 2316 82-83 -0.9328408175345011 2316 84-85 -0.9480336332415078 2316 86-87 -0.7346080328386861 2316 88-89 -0.9646925497486585 2316 90-91 -0.6371297896578021 2316 92-93 -0.7228876136641382 2316 94-95 -0.45702006665430517 2316 96-97 -0.4505741156523513 2316 98-99 -0.5021690801335801 2316 100-101 -0.745171193798285 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 27.0 4 77.0 5 143.0 6 278.0 7 767.0 8 1759.0 9 3080.0 10 5132.0 11 7669.0 12 10364.0 13 13635.0 14 16103.0 15 19682.0 16 23050.0 17 26136.0 18 24078.0 19 21513.0 20 21495.0 21 23173.0 22 25611.0 23 29964.0 24 36513.0 25 45762.0 26 58480.0 27 74223.0 28 93312.0 29 115320.0 30 139480.0 31 168469.0 32 201249.0 33 241850.0 34 296073.0 35 367374.0 36 471980.0 37 589146.0 38 466482.0 39 76698.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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22.318816673201177 18-19 22.076362553136068 27.536612321243904 28.84217797531704 21.54484715030299 20-21 22.61619297518115 26.751959747772368 28.883564705246023 21.74828257180046 22-23 22.32226408585299 26.65551987511862 28.783344341541213 22.23887169748718 24-25 22.308784318611423 26.825904925002362 28.740264959667268 22.125045796718943 26-27 22.19604483088314 26.96532035341362 28.59852495856542 22.24010985713782 28-29 22.199567430111035 27.188315903838973 28.55409310007299 22.058023565977 30-31 22.450188122438224 26.89787566023158 28.50910041117556 22.142835806154633 32-33 22.26738006488195 27.023308355065172 28.391438467789015 22.31787311226386 34-35 22.486311160762167 27.03114977099259 28.45498282360341 22.02755624464184 36-37 22.486018768117745 26.89085523496475 28.411558491096617 22.211567505820888 38-39 22.349429946740493 26.948000400415058 28.46470696506917 22.237862687775287 40-41 22.480044304429338 26.92138574050747 28.449867024819252 22.148702930243935 42-43 22.425446513571174 27.00951122515641 28.189287980144634 22.375754281127783 44-45 22.378216312872194 27.088794408540746 28.250752883266056 22.282236395321004 46-47 22.56060574256264 27.088496951749537 27.927001304124815 22.423896001563012 48-49 22.47718847164543 27.233421232055992 27.778615784174164 22.510774512124414 50-51 22.515003752201814 27.28036071822818 27.743565887167577 22.46106964240243 52-53 22.637253112395143 27.263106089853867 27.680070925079058 22.41956987267193 54-55 22.385733452293003 27.234214630693476 27.824249201664898 22.555802715348623 56-57 22.470976957167338 27.33609675984765 27.610533135888055 22.582393147096955 58-59 22.45508591210039 27.302420201889166 27.72534288873676 22.517150997273685 60-61 22.425748692105948 27.35540067153504 27.81981041829724 22.399040218061767 62-63 22.457623751681965 27.2772414506435 27.767727021796766 22.497407775877765 64-65 22.232157419861945 27.248642081160373 27.724319410902492 22.79488108807519 66-67 22.22931745629169 27.351274293413237 27.67086961634149 22.748538633953583 68-69 22.382382509115672 27.249596475273467 27.714269474782128 22.65375154082873 70-71 22.278625981441348 27.121819595387798 27.69276288474029 22.906791538430564 72-73 22.309661882775202 27.22393865696936 27.613021135642057 22.853378324613377 74-75 22.260075502458463 27.43919996687613 27.4495309452589 22.85119358540651 76-77 22.239102585380767 27.270165961901384 27.50806978512148 22.982661667596368 78-79 22.202285489615427 27.276598819431463 27.51370016831626 23.007415522636848 80-81 22.296174365780423 27.34020928156136 27.405088395438064 22.95852795722015 82-83 22.32409092486963 27.251156904412532 27.52008992060906 22.90466225010878 84-85 22.354256415279508 27.334939468543794 27.219861217216557 23.090942898960137 86-87 22.216816413255916 27.375532369777783 27.345259241578756 23.062391975387545 88-89 22.223510888527645 27.21701358849285 27.272595171519843 23.286880351459665 90-91 22.1926169481956 27.227736694143417 27.335192984689293 23.244453372971687 92-93 22.242002676080542 27.115803933701134 27.386735893619946 23.255457496598382 94-95 22.021054059221488 27.054556247873272 27.46236276440855 23.462026928496687 96-97 22.03778786044868 26.847313374237487 27.51554222401738 23.599356541296448 98-99 21.87131256480131 27.005998384894976 27.349013109475433 23.77367594082828 100-101 21.89141392192547 26.942182630651423 27.332786712760754 23.833616734662357 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 403.0 1 305.5 2 283.5 3 385.0 4 348.0 5 345.0 6 516.5 7 973.5 8 1599.0 9 1928.0 10 1745.0 11 1506.0 12 1637.5 13 1874.0 14 2092.0 15 2389.0 16 2710.5 17 3042.5 18 3409.5 19 3830.5 20 4478.0 21 5307.0 22 6480.0 23 8286.0 24 10853.5 25 14505.0 26 19517.0 27 25697.0 28 33106.0 29 42237.0 30 51154.0 31 60244.5 32 71164.0 33 78295.5 34 82240.5 35 86959.0 36 93525.5 37 100841.5 38 104833.5 39 106811.5 40 109201.5 41 112095.0 42 115026.5 43 119012.0 44 123469.5 45 128435.0 46 132742.5 47 135548.0 48 137893.0 49 138590.0 50 136865.5 51 134941.5 52 134434.0 53 133898.5 54 130234.0 55 120398.5 56 108905.5 57 97382.5 58 85307.0 59 74199.0 60 62528.5 61 51141.5 62 41207.0 63 31400.5 64 22873.5 65 16798.5 66 12109.5 67 9128.0 68 6890.0 69 4971.0 70 3517.0 71 2385.5 72 1652.5 73 1142.5 74 800.0 75 529.0 76 341.0 77 212.5 78 122.0 79 70.0 80 42.5 81 25.5 82 18.0 83 9.5 84 4.0 85 3.5 86 2.5 87 2.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020047538229579026 2 0.003525137594731345 3 0.0033367714637151666 4 0.034982281474433205 5 0.001399291258977328 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 6.727361822006385E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.345472364401277E-5 24-25 4.440058802524214E-4 26-27 0.0018298424155857365 28-29 1.2109251279611491E-4 30-31 0.006350629559974026 32-33 0.02163519561957253 34-35 0.012311072134271685 36-37 6.592814585566257E-4 38-39 6.189172876245873E-4 40-41 0.0016145668372815323 42-43 0.00792483222632352 44-45 0.007682647200731292 46-47 0.007131003531326767 48-49 0.00908193845970862 50-51 0.18867558965999107 52-53 0.0491097413006466 54-55 0.016737676213151885 56-57 0.009943040772925436 58-59 0.019172981192718194 60-61 0.053549800103170814 62-63 0.09745256335358449 64-65 0.10162352768322844 66-67 0.10689777935168146 68-69 0.10438174603025106 70-71 0.09287795731462015 72-73 0.06884782088641334 74-75 0.23870025216843052 76-77 0.2873390781415367 78-79 0.006606269309210269 80-81 6.727361822006385E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 2.1527557830420432E-4 90-91 0.006390993730906065 92-93 0.017719871039164815 94-95 0.0017894782446536983 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3716167.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.91259294593921 #Duplication Level Percentage of deduplicated Percentage of total 1 77.105132095504 43.88252996994292 2 12.79287142057052 14.561509675373385 3 4.107130159278659 7.0124228079305055 4 1.8386362990173388 4.18566237046408 5 1.0583216192171934 3.01159137601977 6 0.6314649380905046 2.156298418870656 7 0.4506881581639163 1.795488218379663 8 0.31964935015646473 1.4553658680711095 9 0.24635002918627172 1.2618377039968673 >10 1.3452852846790757 13.788264053814943 >50 0.07639387928944426 2.938210409838269 >100 0.025896716541693576 2.556974568249667 >500 0.001563949146892214 0.6199030509477412 >1k 5.213163645642691E-4 0.4349004278459631 >5k 9.478479355713943E-5 0.3390410802545112 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6774 0.182284595929085 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5796 0.15596715648139603 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.072834186407662E-5 2 0.0 0.0 0.0 0.0 8.072834186407662E-5 3 0.0 0.0 0.0 0.0 8.072834186407662E-5 4 0.0 0.0 0.0 0.0 8.072834186407662E-5 5 0.0 0.0 0.0 0.0 8.072834186407662E-5 6 0.0 0.0 0.0 0.0 1.6145668372815323E-4 7 0.0 0.0 0.0 0.0 1.6145668372815323E-4 8 0.0 0.0 0.0 0.0 1.6145668372815323E-4 9 0.0 0.0 0.0 0.0 1.8836613101617876E-4 10-11 0.0 0.0 0.0 0.0 2.4218502559222985E-4 12-13 0.0 0.0 0.0 0.0 5.785531166925491E-4 14-15 0.0 0.0 0.0 0.0 7.400098004207023E-4 16-17 0.0 0.0 0.0 0.0 8.7455703686083E-4 18-19 0.0 0.0 0.0 0.0 9.956495496569449E-4 20-21 0.0 0.0 0.0 2.690944728802554E-5 0.0010763778915210214 22-23 0.0 0.0 0.0 9.41830655080894E-5 0.0011301967860970726 24-25 0.0 0.0 0.0 2.0182085466019154E-4 0.0011301967860970726 26-27 0.0 0.0 0.0 4.440058802524214E-4 0.0011974704043171364 28-29 0.0 0.0 0.0 0.0018298424155857365 0.0012109251279611491 30-31 0.0 0.0 0.0 0.006700452374718359 0.0012512892988931876 32-33 0.0 0.0 0.0 0.019307528429158324 0.0013723818116893024 34-35 0.0 0.0 0.0 0.03922051942229722 0.0014800196008414046 36-37 0.0 0.0 0.0 0.07083911998572723 0.0014800196008414046 38-39 0.0 0.0 0.0 0.11745973741223148 0.0015876573899935068 40-41 0.0 0.0 0.0 0.16526437051940884 0.0016145668372815323 42-43 0.0 2.690944728802554E-5 0.0 0.21371483036149883 0.0016145668372815323 44-45 0.0 2.690944728802554E-5 0.0 0.2754316477165854 0.0016549310082135706 46-47 0.0 2.690944728802554E-5 0.0 0.3481678837361184 0.0017222046264336343 48-49 0.0 2.690944728802554E-5 0.0 0.42538454272910775 0.0017625687973656728 50-51 0.0 2.690944728802554E-5 0.0 0.5063147054478445 0.001856751862873762 52-53 0.0 2.690944728802554E-5 0.0 0.5863299469587885 0.0020720274411779663 54-55 0.0 2.690944728802554E-5 0.0 0.6778086130144312 0.0021527557830420432 56-57 0.0 2.690944728802554E-5 0.0 0.782943823568747 0.0022065746776180942 58-59 0.0 2.690944728802554E-5 0.0 0.9019239447527521 0.0023007577431261835 60-61 2.690944728802554E-5 2.690944728802554E-5 0.0 1.0279274316789315 0.0023545766377022345 62-63 2.690944728802554E-5 2.690944728802554E-5 0.0 1.1607659182162697 0.0024218502559222982 64-65 2.690944728802554E-5 2.690944728802554E-5 0.0 1.2998877606953616 0.0024756691504983497 66-67 2.690944728802554E-5 2.690944728802554E-5 0.0 1.457388755672175 0.002556397492362426 68-69 2.690944728802554E-5 2.690944728802554E-5 0.0 1.6332285389757781 0.002610216386938477 70-71 2.690944728802554E-5 2.690944728802554E-5 0.0 1.8294253191527723 0.0027313088997345924 72-73 2.690944728802554E-5 2.690944728802554E-5 0.0 2.030371616776103 0.002973493925326822 74-75 2.690944728802554E-5 2.690944728802554E-5 0.0 2.239336929691265 0.0032291336745630646 76-77 2.690944728802554E-5 2.690944728802554E-5 0.0 2.468726513098039 0.003309862016427141 78-79 2.690944728802554E-5 2.690944728802554E-5 0.0 2.732143092600521 0.003363680911003192 80-81 2.690944728802554E-5 2.690944728802554E-5 0.0 3.025415703869067 0.003404045081935231 82-83 5.381889457605108E-5 2.690944728802554E-5 0.0 3.3290888165144352 0.003511682871087333 84-85 5.381889457605108E-5 2.690944728802554E-5 0.0 3.645476643003396 0.003552047042019371 86-87 5.381889457605108E-5 2.690944728802554E-5 0.0 3.9885855506493653 0.0037000490021035113 88-89 5.381889457605108E-5 2.690944728802554E-5 0.0 4.362909417149445 0.0038884151331196903 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4110 0.0 57.545395 1 GTATCAA 7610 0.0 41.06429 1 TCAACGC 9740 0.0 31.680809 4 CAACGCA 9785 0.0 31.38154 5 ATCAACG 9825 0.0 31.263453 3 TATCAAC 10155 0.0 30.810587 2 AACGCAG 10515 0.0 29.383432 6 GTGGTAT 1980 0.0 25.904884 1 ACGCAGA 12470 0.0 24.51039 7 GTACATG 10435 0.0 24.076107 1 CGCAGAG 12980 0.0 23.364525 8 TACATGG 10945 0.0 22.73055 2 ACATGGG 10620 0.0 22.619928 3 TGGTATC 2200 0.0 20.71777 2 GCAGAGT 14870 0.0 19.916105 9 AGAGTAC 13745 0.0 18.680279 10-11 CATGGGG 7585 0.0 18.400667 4 GAGTACA 8225 0.0 18.246277 1 CAGAGTA 14325 0.0 17.708586 10-11 GAGTACT 9555 0.0 17.310232 12-13 >>END_MODULE