FastQCFastQC Report
Sat 18 Jun 2016
SRR1930958_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930958_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3995987
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN73730.18451010976762436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28900.051.4964871
GTATCAA56350.045.1221771
TCAACGC70350.036.044134
ATCAACG69200.035.85453
TATCAAC71300.034.7336162
CAACGCA70950.034.291125
AACGCAG77100.031.1123856
GTACATG73800.027.070251
ACGCAGA86550.027.0008627
TACATGG75000.026.1605192
CGCAGAG89650.026.1150888
ACATGGG78450.024.105883
GCAGAGT101200.021.5541279
CATGGGG60450.020.2647194
GTGGTAT17150.019.120651
AGAGTAC90450.018.7642710-11
CAGAGTA94350.018.26971410-11
GAGTACT63450.018.038512-13
GAGTACA56200.017.0557461
ATGGGGG37950.016.6965075
Produced by FastQC (version 0.11.5)