FastQCFastQC Report
Sat 18 Jun 2016
SRR1930958_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930958_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3995987
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62010.15518068502224858No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50240.1257261347446826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45350.051.8296851
GTATCAA82750.040.0532841
CAACGCA98700.032.505135
TCAACGC99150.032.3186384
ATCAACG100500.032.073853
AACGCAG104800.030.9297476
TATCAAC105950.030.6047532
ACGCAGA122900.026.104287
GTACATG107100.025.4934751
CGCAGAG125450.025.384518
TACATGG112000.023.7801152
ACATGGG107050.023.7697983
CATGGGG77950.021.1936174
GCAGAGT147100.020.8418759
AGAGTAC132950.019.24054510-11
CAGAGTA137000.019.0181710-11
GAGTACA83350.018.4582311
ATGGGGG47300.018.3616775
GTGGTAT25350.018.3568761
GAGTACT93050.018.15727212-13
Produced by FastQC (version 0.11.5)