##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930958_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3995987 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.255094173229292 31.0 31.0 34.0 30.0 34.0 2 31.376026498584704 31.0 31.0 34.0 30.0 34.0 3 31.462207960136006 31.0 31.0 34.0 30.0 34.0 4 35.32659265408021 37.0 35.0 37.0 33.0 37.0 5 35.11320607399374 37.0 35.0 37.0 32.0 37.0 6 35.15305955700056 37.0 35.0 37.0 32.0 37.0 7 35.03421432552208 37.0 35.0 37.0 32.0 37.0 8 35.01247776832107 36.0 35.0 37.0 32.0 37.0 9 36.519485173500314 38.0 35.0 39.0 32.0 39.0 10-11 36.444223917645374 38.0 35.0 39.0 32.0 39.0 12-13 36.341881367481925 38.0 35.0 39.0 32.0 39.0 14-15 37.365273335473816 39.0 36.0 41.0 32.0 41.0 16-17 37.329061005453724 39.0 36.0 41.0 32.0 41.0 18-19 37.315570596200644 39.0 36.0 40.5 32.0 41.0 20-21 37.279014296092555 39.0 36.0 40.0 31.0 41.0 22-23 37.15241466000765 39.0 36.0 40.0 31.0 41.0 24-25 37.028364206390066 39.0 36.0 40.0 31.0 41.0 26-27 36.75971781189479 39.0 35.5 40.0 30.5 41.0 28-29 36.57731769397648 38.0 35.0 40.0 30.0 41.0 30-31 36.34280942355418 38.0 35.0 40.0 30.0 41.0 32-33 36.106620967485625 38.0 35.0 40.0 30.0 41.0 34-35 36.30827602792502 38.0 35.0 40.0 30.0 41.0 36-37 36.36650757372334 38.0 35.0 40.0 30.0 41.0 38-39 36.337360456878365 38.0 35.0 40.0 30.0 41.0 40-41 36.239282560228546 38.0 35.0 40.0 30.0 41.0 42-43 36.10127422836961 38.0 35.0 40.0 29.5 41.0 44-45 35.92384672422608 38.0 35.0 40.0 29.0 41.0 46-47 35.70757224685666 38.0 34.0 40.0 28.0 41.0 48-49 35.51414719317155 38.0 34.0 40.0 27.5 41.0 50-51 35.24774242258546 38.0 34.0 40.0 27.0 41.0 52-53 35.03458644885481 38.0 33.5 40.0 26.5 41.0 54-55 34.862539592846524 38.0 33.0 40.0 26.0 41.0 56-57 34.61478590896317 37.5 33.0 40.0 26.0 41.0 58-59 34.37299157880143 37.0 33.0 40.0 26.0 41.0 60-61 34.06112619986001 37.0 32.5 40.0 25.0 41.0 62-63 33.752973545709736 36.5 32.0 39.0 24.5 41.0 64-65 33.45046830732933 36.0 32.0 39.0 23.5 41.0 66-67 33.11301863594652 35.5 31.0 39.0 23.0 40.0 68-69 32.741172581392284 35.0 31.0 38.5 22.5 40.0 70-71 32.3382060552249 35.0 31.0 38.0 22.0 40.0 72-73 31.91548408440768 35.0 30.5 37.5 21.5 39.5 74-75 31.389590481650714 34.0 30.0 37.0 20.0 39.0 76-77 30.016558737553453 33.0 28.5 35.5 18.5 38.0 78-79 30.78338518118302 34.0 30.0 36.0 20.0 38.5 80-81 30.78113817687595 34.0 30.0 36.0 20.0 37.5 82-83 30.502670804484598 34.0 30.0 35.0 19.0 37.0 84-85 30.175807378752733 34.0 30.0 35.0 18.0 36.5 86-87 29.832647853959486 34.0 29.0 35.0 16.5 36.0 88-89 29.483174119435326 34.0 29.0 35.0 11.5 36.0 90-91 29.140639721800895 34.0 29.0 35.0 7.0 35.5 92-93 28.777109134739426 33.5 29.0 35.0 2.0 35.0 94-95 28.356257415251854 33.0 29.0 35.0 2.0 35.0 96-97 27.88580756143601 33.0 27.5 35.0 2.0 35.0 98-99 27.395302712446263 33.0 27.0 35.0 2.0 35.0 100-101 26.17010953739339 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5129475631656781 1101 2 0.5608457861781666 1101 3 0.5988745953210319 1101 4 0.40533185050573195 1101 5 0.5076815254595815 1101 6 0.4515557383103115 1101 7 0.5027988273335211 1101 8 0.4808537430922897 1101 9 0.6231589381999072 1101 10-11 0.6597649115130579 1101 12-13 0.7215689748860754 1101 14-15 0.8598420440911454 1101 16-17 0.7633196780369005 1101 18-19 0.8309523938298398 1101 20-21 0.7697961320956068 1101 22-23 0.7083095323432005 1101 24-25 0.6763446413137828 1101 26-27 0.7605548042255563 1101 28-29 0.7192890619491692 1101 30-31 0.7744232013764716 1101 32-33 0.7809325745801843 1101 34-35 0.7459194662583073 1101 36-37 0.8754183841876682 1101 38-39 0.8639948922746896 1101 40-41 0.8228787157795523 1101 42-43 0.758757185016762 1101 44-45 0.7928990135676344 1101 46-47 0.8802568345224202 1101 48-49 0.9013773256857078 1101 50-51 0.7331229846874407 1101 52-53 0.8209252356103534 1101 54-55 0.8466622028736666 1101 56-57 0.9453050684293842 1101 58-59 0.871517171458251 1101 60-61 0.8201124344565045 1101 62-63 0.7886661262121919 1101 64-65 0.7772981342034555 1101 66-67 0.8658783459978707 1101 68-69 0.816480777145749 1101 70-71 0.6716515194785231 1101 72-73 0.7488596156161265 1101 74-75 0.7299306572974054 1101 76-77 0.8399571190187274 1101 78-79 0.8821557826875441 1101 80-81 0.7355450949194875 1101 82-83 0.9434027106428289 1101 84-85 1.014472655059464 1101 86-87 0.9247575371858119 1101 88-89 0.9129853596638675 1101 90-91 0.885127004109048 1101 92-93 0.892733622539815 1101 94-95 0.9557862343754024 1101 96-97 0.9201958125383349 1101 98-99 1.1282294858447983 1101 100-101 1.316741317366084 1102 1 0.4551061102824434 1102 2 0.4993946560640836 1102 3 0.5141186363310872 1102 4 0.3147310492642603 1102 5 0.37673607351134564 1102 6 0.3494510185175699 1102 7 0.45855185796120423 1102 8 0.4423763616065557 1102 9 0.6476411505094148 1102 10-11 0.5536746971777191 1102 12-13 0.5713629506410527 1102 14-15 0.7126570702905681 1102 16-17 0.5688825923741874 1102 18-19 0.7115879977802138 1102 20-21 0.7461293810193865 1102 22-23 0.7326842523307349 1102 24-25 0.7053985725264837 1102 26-27 0.7231319902707085 1102 28-29 0.775136436487351 1102 30-31 0.7559733179265962 1102 32-33 0.7175368106412492 1102 34-35 0.8291591049854148 1102 36-37 0.799383652902236 1102 38-39 0.8594584955238105 1102 40-41 0.7965705624658312 1102 42-43 0.8484200662395338 1102 44-45 0.9247001511487127 1102 46-47 0.969439685098024 1102 48-49 0.7582164151014013 1102 50-51 0.8890794423430677 1102 52-53 0.883602414513156 1102 54-55 0.799863662509658 1102 56-57 0.7744319814420919 1102 58-59 0.8417698482151081 1102 60-61 0.9111095060942489 1102 62-63 0.7485175978603209 1102 64-65 0.7975911652207159 1102 66-67 0.9276830547524426 1102 68-69 0.8971041741758299 1102 70-71 0.9451015977196917 1102 72-73 0.8967662911133374 1102 74-75 0.9611696597063251 1102 76-77 1.1432231519644276 1102 78-79 1.1401332648154465 1102 80-81 1.0721957562358888 1102 82-83 1.1386354212933654 1102 84-85 1.0754906667599258 1102 86-87 1.0917221046517724 1102 88-89 1.110589459371031 1102 90-91 1.0141094793867858 1102 92-93 1.1789270113237293 1102 94-95 1.0565641982870702 1102 96-97 1.0760364018468884 1102 98-99 1.1767498188080303 1102 100-101 1.3728261100107666 1103 1 0.42946735405439895 1103 2 0.5140322437335563 1103 3 0.40583252412362114 1103 4 0.23793329436931288 1103 5 0.28117418167068564 1103 6 0.4092978344019187 1103 7 0.46410328339526075 1103 8 0.3690339770977644 1103 9 0.5611499762880285 1103 10-11 0.5474124096459931 1103 12-13 0.5553105730936849 1103 14-15 0.6102938738148183 1103 16-17 0.6100585378825585 1103 18-19 0.6261453238771466 1103 20-21 0.6680585169599382 1103 22-23 0.6647011181037286 1103 24-25 0.6829104864718545 1103 26-27 0.7025077541882112 1103 28-29 0.7877904311301478 1103 30-31 0.7195825242542426 1103 32-33 0.8160794503581315 1103 34-35 0.7355983310828051 1103 36-37 0.7383272714169422 1103 38-39 0.7575012402956034 1103 40-41 0.8232483946443807 1103 42-43 0.8299023182007303 1103 44-45 0.9021285380884194 1103 46-47 0.8338490675321282 1103 48-49 0.8062354287476978 1103 50-51 0.8816094173486348 1103 52-53 0.7364903339424487 1103 54-55 0.8252457229134933 1103 56-57 0.8868671794575178 1103 58-59 0.9178191381000218 1103 60-61 0.8200750931570298 1103 62-63 0.8032777766976267 1103 64-65 0.7628470513057763 1103 66-67 0.6560040617061134 1103 68-69 0.7492241179473424 1103 70-71 0.6920747208726041 1103 72-73 0.6323510271172523 1103 74-75 0.7106252054676823 1103 76-77 0.878750995045614 1103 78-79 0.9772242417365895 1103 80-81 0.888155251752945 1103 82-83 0.8773820484571182 1103 84-85 0.9204758913054221 1103 86-87 0.8373440445296012 1103 88-89 0.8639773935199777 1103 90-91 0.9880246889484781 1103 92-93 0.8991339212702094 1103 94-95 0.8339303717913822 1103 96-97 0.7224748765164257 1103 98-99 0.9862715570422118 1103 100-101 1.2590427861238673 1104 1 0.48013643807727746 1104 2 0.5750966764480943 1104 3 0.46734902626800334 1104 4 0.28893924303184804 1104 5 0.442260798598781 1104 6 0.3130538326006942 1104 7 0.40310386604227233 1104 8 0.4231829020140765 1104 9 0.4666034222814375 1104 10-11 0.5390907471776742 1104 12-13 0.5306466614985439 1104 14-15 0.6256114626150193 1104 16-17 0.6630252161730041 1104 18-19 0.6997319886553228 1104 20-21 0.5467353559179102 1104 22-23 0.6160886765575455 1104 24-25 0.6380841567816304 1104 26-27 0.7717492053821289 1104 28-29 0.6320008648318307 1104 30-31 0.5265482397097188 1104 32-33 0.673225966242633 1104 34-35 0.6793601952469928 1104 36-37 0.6778923332310498 1104 38-39 0.6675489937748509 1104 40-41 0.6554141857607192 1104 42-43 0.612425660386485 1104 44-45 0.69800922554262 1104 46-47 0.6770995524586212 1104 48-49 0.7420498941066285 1104 50-51 0.790488474132502 1104 52-53 0.8084093546968703 1104 54-55 0.7798060917729828 1104 56-57 0.7982102273011051 1104 58-59 0.7948507238386711 1104 60-61 0.7710593437021629 1104 62-63 0.7311368834266645 1104 64-65 0.7787253824088367 1104 66-67 0.7384215139193628 1104 68-69 0.7249514529998677 1104 70-71 0.6046353788377843 1104 72-73 0.5771956400993332 1104 74-75 0.8848129038442814 1104 76-77 0.9659164826556612 1104 78-79 0.908974092520257 1104 80-81 0.7597683539645672 1104 82-83 0.9054400049732649 1104 84-85 0.9888822292945214 1104 86-87 0.9926473627617511 1104 88-89 1.0519855349015295 1104 90-91 0.9251353904830673 1104 92-93 0.9396146424230309 1104 94-95 1.0508808001302086 1104 96-97 0.9217557093984503 1104 98-99 1.1141775842031976 1104 100-101 1.4838039898656632 1105 1 0.145150856326417 1105 2 0.29731481360463974 1105 3 0.3007579956086879 1105 4 0.11390522748336451 1105 5 0.19433802498842567 1105 6 0.16850634089921357 1105 7 0.2808621115670178 1105 8 0.26365416961612453 1105 9 0.37136059469943206 1105 10-11 0.3349723459226155 1105 12-13 0.24566744733561308 1105 14-15 0.48247558076723607 1105 16-17 0.38421996569250183 1105 18-19 0.3712860495358399 1105 20-21 0.4036355905095306 1105 22-23 0.38612533356326395 1105 24-25 0.3471308586425508 1105 26-27 0.3663668113673495 1105 28-29 0.44154976224178455 1105 30-31 0.4512102618337508 1105 32-33 0.4552848535069316 1105 34-35 0.4719800723189067 1105 36-37 0.309212090481374 1105 38-39 0.35168013256630815 1105 40-41 0.38946305342772547 1105 42-43 0.32401582708391174 1105 44-45 0.40790422112603153 1105 46-47 0.3559639465593136 1105 48-49 0.3749847997719087 1105 50-51 0.46039967962371264 1105 52-53 0.4977863177905846 1105 54-55 0.41691861313007195 1105 56-57 0.41180348136396816 1105 58-59 0.38845686297241144 1105 60-61 0.4096867785521283 1105 62-63 0.3766418738687918 1105 64-65 0.29071627634500175 1105 66-67 0.397298645556468 1105 68-69 0.3900093939190441 1105 70-71 0.31033983470939575 1105 72-73 0.343198999636364 1105 74-75 0.5297862095465753 1105 76-77 0.4257889644782331 1105 78-79 0.502321308650945 1105 80-81 0.3797877671408827 1105 82-83 0.5041454076621221 1105 84-85 0.587843309227992 1105 86-87 0.5575584596675824 1105 88-89 0.6405068098448901 1105 90-91 0.41781422823244085 1105 92-93 0.37294192487577504 1105 94-95 0.2420380322229434 1105 96-97 0.17466290572883025 1105 98-99 0.6123964004904394 1105 100-101 0.9695533989015708 1106 1 0.1708919504696702 1106 2 0.21758174316042656 1106 3 0.17921437048839195 1106 4 0.07901632694883176 1106 5 0.1515913758718952 1106 6 0.11859682530213433 1106 7 0.20825940944435217 1106 8 0.15446279413976782 1106 9 0.24248389895343792 1106 10-11 0.21438083745902503 1106 12-13 0.14058555764649583 1106 14-15 0.1993186676318075 1106 16-17 0.18617014448342672 1106 18-19 0.22288530492532033 1106 20-21 0.25817989939562835 1106 22-23 0.298446037873795 1106 24-25 0.2068057647551953 1106 26-27 0.29091036820181415 1106 28-29 0.2635282218040871 1106 30-31 0.32305155988168366 1106 32-33 0.2732058923953744 1106 34-35 0.26000813620904495 1106 36-37 0.28845606079676855 1106 38-39 0.25959937810085165 1106 40-41 0.33237717298737124 1106 42-43 0.3346071642657549 1106 44-45 0.2530196400675706 1106 46-47 0.2401976490445108 1106 48-49 0.39614816240235484 1106 50-51 0.3897892904670428 1106 52-53 0.2430597923508273 1106 54-55 0.372876858497186 1106 56-57 0.402689449237414 1106 58-59 0.32446588940697296 1106 60-61 0.32954872886816133 1106 62-63 0.3195014895352415 1106 64-65 0.2863139951897651 1106 66-67 0.14439374685317574 1106 68-69 0.14294480572799984 1106 70-71 0.0350878860676076 1106 72-73 0.13233631133420687 1106 74-75 0.3588692884527873 1106 76-77 0.3425346646258305 1106 78-79 0.42614304337431363 1106 80-81 0.3780999046417861 1106 82-83 0.4366552954964007 1106 84-85 0.3333868361165031 1106 86-87 0.2939453608563909 1106 88-89 0.4746689880223691 1106 90-91 0.5160456421212807 1106 92-93 0.46802314418401636 1106 94-95 0.5693399794808727 1106 96-97 0.5887532567361724 1106 98-99 0.5302447694118086 1106 100-101 0.7605976662716962 1107 1 0.09816762395962542 1107 2 0.143760330193345 1107 3 0.15015694875987862 1107 4 -0.011231418105616342 1107 5 0.15799377429174655 1107 6 0.15471841348571047 1107 7 0.18200717653008525 1107 8 0.014525612898971474 1107 9 0.11685721651048908 1107 10-11 0.12072371545560401 1107 12-13 0.05078294419902818 1107 14-15 0.08995417447784604 1107 16-17 0.061617024495923545 1107 18-19 0.14980933467358426 1107 20-21 0.2310923847470363 1107 22-23 0.22982506018090731 1107 24-25 0.23435496903304198 1107 26-27 0.19504011034060653 1107 28-29 0.24622495032036085 1107 30-31 0.20077009550677616 1107 32-33 0.24865609506944253 1107 34-35 0.0911924649227771 1107 36-37 0.1490860540148944 1107 38-39 0.1642778016820401 1107 40-41 0.04954940113159978 1107 42-43 0.1908927083825418 1107 44-45 0.21438825376415593 1107 46-47 0.1352135958850198 1107 48-49 0.10727007815281553 1107 50-51 0.10988573721498796 1107 52-53 0.08644144027928036 1107 54-55 0.18058818654510844 1107 56-57 0.11170979537028813 1107 58-59 0.27363707331772247 1107 60-61 0.09879074020292222 1107 62-63 -0.066112513719645 1107 64-65 0.03512520382642492 1107 66-67 0.061840534096525346 1107 68-69 0.13292493554842366 1107 70-71 -0.010653332555286 1107 72-73 0.0032985104474363425 1107 74-75 0.2697939011318944 1107 76-77 0.22392670391572267 1107 78-79 0.26119691831364733 1107 80-81 0.30696489948447336 1107 82-83 0.28250660423529084 1107 84-85 0.3131341911037495 1107 86-87 0.33741403929789016 1107 88-89 0.42120363168958264 1107 90-91 0.3389244502798121 1107 92-93 0.43368197185724 1107 94-95 0.49509928090150623 1107 96-97 0.6047118177837767 1107 98-99 0.4136990979359041 1107 100-101 0.7144202999437255 1108 1 0.27714205580006634 1108 2 0.3418844832015182 1108 3 0.3317026794278881 1108 4 0.07126237352065345 1108 5 0.23068595496017963 1108 6 0.25833144342361436 1108 7 0.2696957326447489 1108 8 0.3450769708449286 1108 9 0.29213854341991663 1108 10-11 0.33425659204053915 1108 12-13 0.31973266967380454 1108 14-15 0.39268386978463354 1108 16-17 0.45470301762996 1108 18-19 0.4551955930858256 1108 20-21 0.48241180286984076 1108 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-0.9715099192329113 2316 90-91 -0.8660753319311958 2316 92-93 -1.0959014138009593 2316 94-95 -0.8713658640111106 2316 96-97 -0.7408704321064654 2316 98-99 -0.7540465548227644 2316 100-101 -0.9368613213363055 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 27.0 4 78.0 5 121.0 6 267.0 7 711.0 8 1713.0 9 2989.0 10 4836.0 11 7452.0 12 10439.0 13 13079.0 14 15509.0 15 19070.0 16 22244.0 17 25529.0 18 23681.0 19 21337.0 20 21755.0 21 23310.0 22 26273.0 23 30865.0 24 37693.0 25 47441.0 26 61025.0 27 77497.0 28 98152.0 29 122715.0 30 147704.0 31 178953.0 32 213959.0 33 257819.0 34 316134.0 35 391685.0 36 503718.0 37 642311.0 38 537389.0 39 90484.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.62202923421261 20.949608803804182 14.393837377120741 24.034524584862467 2 15.372995601923803 21.611461725925825 39.763649076864475 23.251893595285893 3 18.325050589885826 26.33947452587154 30.807826504345247 24.527648379897386 4 12.918002335673595 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24-25 22.078446794951763 27.18893374936921 28.792374416271194 21.94024503940783 26-27 22.005998869596848 27.31851830348434 28.654618792996207 22.020864033922606 28-29 21.99226498501183 27.471865819299353 28.639963443301646 21.895905752387176 30-31 22.293068311814224 27.247931615358418 28.56001287367506 21.8989871991523 32-33 22.098690310036385 27.337587831627598 28.475469219632632 22.088252638703388 34-35 22.28811162557878 27.357014140908518 28.53535227130522 21.81952196220748 36-37 22.335700554008717 27.221865751610498 28.52335330180487 21.919080392575914 38-39 22.167476651417932 27.344905854913463 28.48471956676243 22.002897926906176 40-41 22.30823940597216 27.183790813950377 28.565279422168732 21.94269035790873 42-43 22.21431231436257 27.304281711403185 28.328447237674975 22.152958736559274 44-45 22.189936097498897 27.374238759124975 28.350313893960667 22.085511249415465 46-47 22.347553784212455 27.33636366480321 28.07000003128353 22.246082519700806 48-49 22.29833711286875 27.485076862510923 27.95586490377889 22.26072112084144 50-51 22.284637222214908 27.52589255100627 27.866935198261437 22.32253502851738 52-53 22.37800975861841 27.505478206353118 27.84725268452141 22.26925935050706 54-55 22.221353152135976 27.41753154088254 28.004754550343208 22.35636075663828 56-57 22.264101502360777 27.611368122251122 27.77673705263888 22.347793322749222 58-59 22.29094142935951 27.529645248200108 27.902626222578647 22.276787099861735 60-61 22.330292777016815 27.60169626160678 27.909144616780612 22.158866344595797 62-63 22.282058559257013 27.54586378623945 27.92133099103653 22.25074666346701 64-65 22.13326750032597 27.444861411875017 27.872287472802864 22.549583614996145 66-67 22.12886515422037 27.495895882554976 27.833231414103633 22.542007549121017 68-69 22.13874434639228 27.491379536022446 27.87341179482638 22.49646432275889 70-71 22.159425435704367 27.33469973329526 27.828557508718472 22.6773173222819 72-73 22.171064106749128 27.43445661228764 27.713107024722554 22.681372256240678 74-75 22.12889479008916 27.60728209005507 27.67735814647012 22.58646497338565 76-77 22.05567849522844 27.4637775550725 27.68227050961276 22.798273440086298 78-79 21.997537127348483 27.475770761389533 27.70129842015516 22.825393691106818 80-81 22.08393529563241 27.63468043591038 27.535079814382478 22.74630445407473 82-83 22.17387093601656 27.485424752382826 27.651003369130077 22.689700942470534 84-85 22.11506193588718 27.51441633819129 27.487777112388002 22.88274461353353 86-87 22.01335990332301 27.56511720383475 27.579819453867096 22.841703438975152 88-89 21.98979575238433 27.423890441976358 27.54523730456311 23.041076501076205 90-91 22.070940974035345 27.40788763885505 27.555056192710346 22.96611519439926 92-93 22.07153309572983 27.292002138006012 27.622114070454835 23.014350695809323 94-95 21.897258563329626 27.233557911617158 27.633752209120573 23.235431315932644 96-97 21.863171727035148 27.102277860263307 27.67631626429215 23.358234148409394 98-99 21.70784089137427 27.139652856728514 27.581383523019472 23.571122728877747 100-101 21.720028118209594 27.120158298813283 27.574063679386345 23.585749903590777 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 346.0 1 274.0 2 282.5 3 381.0 4 341.5 5 341.0 6 507.5 7 908.0 8 1381.5 9 1614.5 10 1521.5 11 1416.0 12 1532.0 13 1707.0 14 1928.0 15 2206.5 16 2529.5 17 2840.5 18 3197.5 19 3625.0 20 4241.0 21 5134.0 22 6411.5 23 8098.0 24 10588.0 25 14310.5 26 19189.5 27 25548.5 28 33446.5 29 43551.0 30 54956.0 31 66664.0 32 79417.0 33 88516.5 34 95002.5 35 101483.5 36 110102.5 37 119938.5 38 125380.0 39 128349.0 40 130428.5 41 132544.0 42 134717.0 43 137870.5 44 141559.0 45 145207.5 46 147711.5 47 148962.0 48 149546.0 49 147949.0 50 143677.5 51 139524.5 52 136795.0 53 133104.5 54 127698.0 55 118372.0 56 106013.0 57 93587.5 58 82367.0 59 71278.0 60 59610.5 61 48819.0 62 39262.5 63 29817.0 64 21772.0 65 16037.5 66 11688.0 67 8859.0 68 6613.0 69 4729.5 70 3457.5 71 2436.0 72 1627.5 73 1122.0 74 790.0 75 556.0 76 346.0 77 199.0 78 121.5 79 70.5 80 38.5 81 24.0 82 11.5 83 10.0 84 7.5 85 3.5 86 4.0 87 3.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020145210682617338 2 0.0037287408592670598 3 0.0032282387305063807 4 0.035585701354884286 5 0.0019769834086046825 6 7.507531931410187E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.2512553219016978E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.5025106438033956E-5 24-25 4.3793936266559426E-4 26-27 0.001689194684567292 28-29 6.25627660950849E-5 30-31 0.005905925119376014 32-33 0.021096164727262626 34-35 0.012187226835322537 36-37 5.255272351987131E-4 38-39 6.756778738269168E-4 40-41 0.002002008515042717 42-43 0.0073824063992200175 44-45 0.0072697934202488644 46-47 0.006869391717240321 48-49 0.00865868682755975 50-51 0.19092904956898007 52-53 0.0495246856408692 54-55 0.01642898237656929 56-57 0.00939692746748175 58-59 0.01859365408345923 60-61 0.053691365862801856 62-63 0.09744776446970424 64-65 0.10181464554314115 66-67 0.1067070538517768 68-69 0.10454238214488686 70-71 0.09370651105721815 72-73 0.07035808675053247 74-75 0.24034112223087814 76-77 0.29149243979021955 78-79 0.006168688736975371 80-81 7.007029802649508E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.0010042575213582E-4 90-91 0.005718236821090759 92-93 0.016904459398891937 94-95 0.0013263306412157998 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3995987.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.31289877472108 #Duplication Level Percentage of deduplicated Percentage of total 1 77.38181170596614 45.12357753014531 2 12.837828169827132 14.972219491087845 3 4.003809869600142 7.004212789176669 4 1.8057784256635119 4.212006981811663 5 0.9993592419573627 2.9137767157920837 6 0.6331900257201706 2.215388752499001 7 0.4402279994859021 1.796967954027356 8 0.31646222566426335 1.4763063784946504 9 0.22857062034237632 1.1995753902210173 >10 1.2570443575211225 13.02833223763677 >50 0.07051263305248165 2.763351502230235 >100 0.023985824959173115 2.395324744799757 >500 0.0011609184082140077 0.4526138888364981 >1k 1.719878880683157E-4 0.16490367911451623 >5k 8.599394403415342E-5 0.2814419641265595 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6201 0.15518068502224858 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5024 0.1257261347446826 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.5025106438033956E-5 2 0.0 0.0 0.0 0.0 2.5025106438033956E-5 3 0.0 0.0 0.0 0.0 2.5025106438033956E-5 4 0.0 0.0 0.0 0.0 2.5025106438033956E-5 5 0.0 0.0 0.0 0.0 2.5025106438033956E-5 6 0.0 0.0 0.0 0.0 5.005021287606791E-5 7 0.0 0.0 0.0 0.0 5.005021287606791E-5 8 0.0 0.0 0.0 0.0 5.005021287606791E-5 9 0.0 0.0 0.0 2.5025106438033956E-5 5.005021287606791E-5 10-11 0.0 0.0 0.0 7.507531931410187E-5 5.005021287606791E-5 12-13 0.0 0.0 0.0 1.1261297897115281E-4 2.0020085150427168E-4 14-15 0.0 0.0 0.0 1.3763808540918676E-4 2.752761708183735E-4 16-17 0.0 0.0 0.0 1.751757450662377E-4 3.003012772564075E-4 18-19 0.0 0.0 0.0 2.1271340472328864E-4 3.003012772564075E-4 20-21 0.0 0.0 0.0 2.752761708183735E-4 3.1281383047542443E-4 22-23 0.0 0.0 0.0 3.378389369134584E-4 3.253263836944414E-4 24-25 0.0 0.0 0.0 5.50552341636747E-4 3.253263836944414E-4 26-27 0.0 0.0 0.0 8.883912785502055E-4 3.628640433514924E-4 28-29 0.0 0.0 0.0 0.0020520587279187845 4.129142562275603E-4 30-31 0.0 0.0 0.0 0.0060685883112232344 4.5045191588461125E-4 32-33 0.0 0.0 0.0 0.017367423867995568 5.255272351987131E-4 34-35 0.0 0.0 0.0 0.03429690837332554 5.75577448074781E-4 36-37 0.0 0.0 0.0 0.060635832899356285 5.75577448074781E-4 38-39 0.0 0.0 0.0 0.09991273745385057 6.00602554512815E-4 40-41 0.0 0.0 0.0 0.14697245011057342 6.00602554512815E-4 42-43 0.0 0.0 0.0 0.19462025276859007 6.256276609508489E-4 44-45 0.0 0.0 0.0 0.251339656510394 6.756778738269168E-4 46-47 0.0 0.0 0.0 0.31715568644242337 7.257280867029848E-4 48-49 0.0 0.0 0.0 0.38757633595905094 8.008034060170866E-4 50-51 0.0 0.0 0.0 0.4648538646396998 8.508536188931545E-4 52-53 0.0 0.0 0.0 0.5435953620469736 9.384414914262734E-4 54-55 0.0 0.0 0.0 0.6300070545775048 0.001063567023616443 56-57 0.0 0.0 0.0 0.7316089867159228 0.0011136172364925112 58-59 0.0 0.0 0.0 0.8440342773887903 0.001163667449368579 60-61 0.0 1.2512553219016978E-5 0.0 0.9652934306342839 0.001176180002587596 62-63 0.0 2.5025106438033956E-5 0.0 1.0922958458073062 0.00120120510902563 64-65 0.0 2.5025106438033956E-5 0.0 1.2275190084452228 0.001251255321901698 66-67 2.5025106438033956E-5 2.5025106438033956E-5 0.0 1.3800720572914775 0.0012762804283397318 68-69 2.5025106438033956E-5 2.5025106438033956E-5 0.0 1.5518318753289237 0.0013013055347777657 70-71 2.5025106438033956E-5 2.5025106438033956E-5 0.0 1.7416222825549732 0.0013513557476538336 72-73 2.5025106438033956E-5 2.5025106438033956E-5 0.0 1.9424237366137578 0.0014639687266249865 74-75 2.5025106438033956E-5 2.5025106438033956E-5 0.0 2.1488433270678806 0.0014764812798440034 76-77 2.5025106438033956E-5 2.5025106438033956E-5 0.0 2.3745822996921664 0.0015265314927200714 78-79 2.5025106438033956E-5 3.753765965705094E-5 0.0 2.633041598984181 0.0015265314927200714 80-81 2.5025106438033956E-5 5.005021287606791E-5 0.0 2.919929419189802 0.0015265314927200714 82-83 2.5025106438033956E-5 5.005021287606791E-5 0.0 3.2304034022132706 0.0015765817055961393 84-85 2.5025106438033956E-5 5.005021287606791E-5 0.0 3.5503118503638778 0.0015765817055961393 86-87 2.5025106438033956E-5 5.005021287606791E-5 0.0 3.887487621956728 0.0016141193652531903 88-89 2.5025106438033956E-5 5.005021287606791E-5 0.0 4.262025877461563 0.001664169578129258 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4535 0.0 51.829685 1 GTATCAA 8275 0.0 40.053284 1 CAACGCA 9870 0.0 32.50513 5 TCAACGC 9915 0.0 32.318638 4 ATCAACG 10050 0.0 32.07385 3 AACGCAG 10480 0.0 30.929747 6 TATCAAC 10595 0.0 30.604753 2 ACGCAGA 12290 0.0 26.10428 7 GTACATG 10710 0.0 25.493475 1 CGCAGAG 12545 0.0 25.38451 8 TACATGG 11200 0.0 23.780115 2 ACATGGG 10705 0.0 23.769798 3 CATGGGG 7795 0.0 21.193617 4 GCAGAGT 14710 0.0 20.841875 9 AGAGTAC 13295 0.0 19.240545 10-11 CAGAGTA 13700 0.0 19.01817 10-11 GAGTACA 8335 0.0 18.458231 1 ATGGGGG 4730 0.0 18.361677 5 GTGGTAT 2535 0.0 18.356876 1 GAGTACT 9305 0.0 18.157272 12-13 >>END_MODULE