FastQCFastQC Report
Sat 18 Jun 2016
SRR1930957_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930957_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4307460
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN78240.18163836692621638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33400.051.2304421
GTATCAA61850.044.020321
ATCAACG75350.035.112023
TCAACGC76750.034.9801064
CAACGCA77350.033.714585
TATCAAC78400.033.690252
AACGCAG84950.030.9654356
GTACATG96200.027.2557581
ACGCAGA98100.026.5506637
TACATGG100100.026.1354682
CGCAGAG101300.025.5464388
ACATGGG106350.024.3096623
GCAGAGT114150.021.5733579
CATGGGG79000.020.7466854
GTGGTAT16200.019.260711
GAGTACA74050.019.0295541
AGAGTAC100100.018.6804910-11
AGTACAT74900.018.2704012
CAGAGTA107650.018.17204910-11
GAGTACT58600.018.0434612-13
Produced by FastQC (version 0.11.5)