##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930957_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4307460 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.987689032515682 30.0 10.0 31.0 2.0 33.0 2 21.87513406973019 30.0 10.0 31.0 2.0 33.0 3 22.333064497406824 30.0 10.0 31.0 2.0 33.0 4 24.294733323118496 33.0 10.0 35.0 2.0 37.0 5 24.283618187980853 32.0 10.0 35.0 2.0 37.0 6 25.021776870824105 32.0 16.0 35.0 2.0 37.0 7 25.099057913480333 32.0 16.0 35.0 2.0 37.0 8 25.8807259962948 33.0 17.0 35.0 2.0 37.0 9 27.03026238200703 33.0 17.0 37.0 2.0 39.0 10-11 27.279247746932064 33.5 17.5 37.0 2.0 39.0 12-13 27.21514291485005 34.0 18.0 37.0 2.0 39.0 14-15 27.44058308144475 34.0 17.0 38.0 2.0 40.0 16-17 26.567992273869052 33.0 14.0 38.0 2.0 40.0 18-19 25.95080940972174 32.5 10.0 38.0 2.0 40.0 20-21 25.438482771749477 32.0 10.0 38.0 2.0 40.0 22-23 25.363761938590258 32.0 10.0 38.0 2.0 40.0 24-25 25.072529634633867 32.0 10.0 38.0 2.0 40.0 26-27 24.730573934522898 31.5 8.5 38.0 2.0 40.0 28-29 24.658280053674325 31.0 8.5 38.0 2.0 40.0 30-31 24.579090113431118 31.0 8.0 37.5 2.0 39.5 32-33 24.279410836084374 31.0 5.0 37.0 2.0 39.0 34-35 21.374475793158844 25.5 2.0 36.0 2.0 39.0 36-37 19.74100618461924 18.0 2.0 35.0 2.0 38.5 38-39 18.805600400235868 18.5 2.0 34.0 2.0 38.0 40-41 20.137367729473983 23.5 2.0 35.0 2.0 38.0 42-43 20.06661837834826 23.5 2.0 35.0 2.0 38.0 44-45 18.11609533228399 18.5 2.0 34.0 2.0 38.0 46-47 18.937311431795074 19.0 2.0 33.0 2.0 38.0 48-49 18.368025007777206 18.5 2.0 33.5 2.0 38.0 50-51 16.384870434084124 11.0 2.0 31.5 2.0 37.5 52-53 19.267986237829255 22.5 2.0 33.5 2.0 37.5 54-55 20.94643026284632 26.5 2.0 35.5 2.0 38.5 56-57 21.17473128015118 28.0 2.0 36.0 2.0 39.0 58-59 20.80036297493186 27.5 2.0 36.0 2.0 39.0 60-61 20.473479498358664 26.0 2.0 35.0 2.0 39.0 62-63 19.991371481104874 26.0 2.0 35.0 2.0 38.5 64-65 19.485606482706746 25.0 2.0 34.0 2.0 38.0 66-67 19.056950499830528 23.5 2.0 34.0 2.0 38.0 68-69 18.590597010767368 22.5 2.0 34.0 2.0 37.0 70-71 18.00160581874237 20.5 2.0 33.0 2.0 36.5 72-73 17.52565084759928 19.5 2.0 32.5 2.0 36.0 74-75 16.982506395880634 17.0 2.0 32.0 2.0 35.5 76-77 16.412291234277276 10.5 2.0 31.5 2.0 35.0 78-79 15.915980647527777 7.5 2.0 31.0 2.0 35.0 80-81 15.396248601263853 4.5 2.0 31.0 2.0 34.0 82-83 14.906141670497231 2.0 2.0 31.0 2.0 34.0 84-85 14.379875959382094 2.0 2.0 30.0 2.0 34.0 86-87 13.811717578340833 2.0 2.0 29.5 2.0 34.0 88-89 13.249836562614625 2.0 2.0 29.0 2.0 34.0 90-91 12.664458985109555 2.0 2.0 29.0 2.0 34.0 92-93 12.029386343692106 2.0 2.0 28.0 2.0 34.0 94-95 11.365656326466176 2.0 2.0 26.5 2.0 33.5 96-97 10.629105783919062 2.0 2.0 25.0 2.0 33.0 98-99 9.820301175170517 2.0 2.0 23.5 2.0 32.0 100-101 8.75368569876447 2.0 2.0 10.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.875882907876214 1101 2 8.029699830858547 1101 3 7.50508171456487 1101 4 9.375083618402154 1101 5 9.249651743439646 1101 6 8.657899364479178 1101 7 8.497135936517893 1101 8 7.654194415922166 1101 9 7.735546761290138 1101 10-11 7.1672292685484145 1101 12-13 6.946492540658653 1101 14-15 7.620492604630666 1101 16-17 8.220058398092963 1101 18-19 8.661944124522027 1101 20-21 9.071398896615367 1101 22-23 9.033152317993807 1101 24-25 8.989835177546727 1101 26-27 8.98410677472458 1101 28-29 8.939174248371057 1101 30-31 8.801200806269517 1101 32-33 8.741167343222997 1101 34-35 11.214495754361156 1101 36-37 5.290806848074844 1101 38-39 8.456191626220434 1101 40-41 10.14499646762589 1101 42-43 10.474721856350282 1101 44-45 4.584427041826405 1101 46-47 6.328150918177336 1101 48-49 9.451396105591257 1101 50-51 -3.657188626778172 1101 52-53 5.921641547826727 1101 54-55 8.512422534897748 1101 56-57 9.153641805115274 1101 58-59 9.171005453384307 1101 60-61 8.958918973981486 1101 62-63 9.094537917858805 1101 64-65 9.132010936201098 1101 66-67 9.04066275651956 1101 68-69 8.98527351106696 1101 70-71 9.148129973156923 1101 72-73 9.03470990964453 1101 74-75 9.121404818738792 1101 76-77 8.971739094275907 1101 78-79 8.899545591372512 1101 80-81 8.710387217566245 1101 82-83 8.516393631973719 1101 84-85 8.504493050223935 1101 86-87 8.310626446783473 1101 88-89 8.049232023946656 1101 90-91 7.800345344281377 1101 92-93 7.66821804538276 1101 94-95 7.477774798156084 1101 96-97 7.366305429393302 1101 98-99 7.13923543081817 1101 100-101 6.52923315120635 1102 1 6.507358582972568 1102 2 7.553864174747325 1102 3 7.281935887627739 1102 4 9.005975879381662 1102 5 9.07390267275225 1102 6 8.431446248330616 1102 7 8.152107124402221 1102 8 7.231657547615004 1102 9 7.184100353598097 1102 10-11 6.7736876386473135 1102 12-13 6.437103018260466 1102 14-15 6.887829230546981 1102 16-17 7.648874019086524 1102 18-19 7.935763375737398 1102 20-21 8.420162082862266 1102 22-23 8.187032324053586 1102 24-25 8.108307728476607 1102 26-27 8.223098761644493 1102 28-29 8.100577194128856 1102 30-31 7.916653745943375 1102 32-33 8.03340372505626 1102 34-35 10.360920912563888 1102 36-37 1.0192535643961769 1102 38-39 3.2596327420885736 1102 40-41 8.067402390084997 1102 42-43 9.285308082781036 1102 44-45 1.0288084979603518 1102 46-47 1.550595882644675 1102 48-49 7.154905393882441 1102 50-51 -4.930624811024217 1102 52-53 4.508965103332859 1102 54-55 7.384698559075591 1102 56-57 7.890869548551667 1102 58-59 8.085475553813726 1102 60-61 7.93574095451125 1102 62-63 8.119299979180145 1102 64-65 8.017654315458152 1102 66-67 7.965784999505093 1102 68-69 7.764539908106055 1102 70-71 7.670987175299441 1102 72-73 7.702784647088031 1102 74-75 7.620939658795489 1102 76-77 7.493439843124996 1102 78-79 7.58507057543741 1102 80-81 7.39226666922144 1102 82-83 7.209576933598013 1102 84-85 7.0759593657139845 1102 86-87 6.921343175860947 1102 88-89 6.763786393065677 1102 90-91 6.765599669322009 1102 92-93 6.524447562430298 1102 94-95 6.301285984154788 1102 96-97 6.2940860745493925 1102 98-99 5.845778331498362 1102 100-101 5.26518125152457 1103 1 6.62141445215557 1103 2 7.631080782830377 1103 3 7.401338885263208 1103 4 9.087360815007013 1103 5 9.031181387187981 1103 6 8.24522199140786 1103 7 8.041973792969863 1103 8 7.234483709430929 1103 9 7.32219030602824 1103 10-11 6.739382438121456 1103 12-13 6.584811742555502 1103 14-15 7.059137091642139 1103 16-17 7.6885613972436 1103 18-19 8.217845700911806 1103 20-21 8.411621197005157 1103 22-23 8.433426813055359 1103 24-25 8.396769598237423 1103 26-27 8.393111684296507 1103 28-29 8.346532702067694 1103 30-31 8.160806869666768 1103 32-33 8.154181709894814 1103 34-35 10.280129610027497 1103 36-37 -3.2226508426321168 1103 38-39 -1.795098604966217 1103 40-41 6.565980546882351 1103 42-43 8.963350064370513 1103 44-45 0.832033720418675 1103 46-47 0.5272873124472106 1103 48-49 7.388358122144179 1103 50-51 -5.141976061814557 1103 52-53 4.501157601739308 1103 54-55 7.697530424798817 1103 56-57 8.231960086916953 1103 58-59 8.338097460104894 1103 60-61 8.374416698519177 1103 62-63 8.365927758089924 1103 64-65 8.280031997289626 1103 66-67 8.142793995658991 1103 68-69 8.167783605639958 1103 70-71 8.24962166622596 1103 72-73 8.033113379672105 1103 74-75 7.973314201052222 1103 76-77 7.91600680141271 1103 78-79 7.715261616233661 1103 80-81 7.543007518762051 1103 82-83 7.357477784817812 1103 84-85 7.214467061780972 1103 86-87 7.03657782120187 1103 88-89 6.97706601699789 1103 90-91 6.945067999022699 1103 92-93 6.759810198659226 1103 94-95 6.418156901395754 1103 96-97 6.106915638313852 1103 98-99 5.835504120189041 1103 100-101 5.3968543824875095 1104 1 6.314180605727753 1104 2 7.468965382060837 1104 3 6.811408236486912 1104 4 8.836182103862964 1104 5 8.76844223330217 1104 6 7.947889677074421 1104 7 7.630202416086618 1104 8 6.7426948908297355 1104 9 6.900993696825324 1104 10-11 6.3588233749631975 1104 12-13 5.8742756882805764 1104 14-15 6.412986186774294 1104 16-17 7.16104147253694 1104 18-19 7.701192406358079 1104 20-21 7.907739271486786 1104 22-23 7.66911008571925 1104 24-25 7.787665703556645 1104 26-27 7.791034422388499 1104 28-29 7.660892999787993 1104 30-31 7.257278065786693 1104 32-33 7.165882615532915 1104 34-35 9.56479124583431 1104 36-37 -3.4924638378068185 1104 38-39 -2.331855291256833 1104 40-41 5.608797677900927 1104 42-43 7.953883339907126 1104 44-45 0.15025068019798837 1104 46-47 -0.47776520987304494 1104 48-49 6.4680240311815815 1104 50-51 -5.603719287985932 1104 52-53 3.4583201243105 1104 54-55 6.207276234189191 1104 56-57 6.833083925730367 1104 58-59 7.031522546705514 1104 60-61 6.903383422546572 1104 62-63 7.001037853000117 1104 64-65 6.997770051015969 1104 66-67 6.759770255564423 1104 68-69 6.864577742889669 1104 70-71 6.828374941876898 1104 72-73 6.527663278807196 1104 74-75 6.422184641022028 1104 76-77 6.289755557738385 1104 78-79 6.09799091470591 1104 80-81 5.949388937800146 1104 82-83 5.872526159377008 1104 84-85 5.8138660578890935 1104 86-87 5.557830179568814 1104 88-89 5.468513064351059 1104 90-91 5.4661466862451835 1104 92-93 5.280071444628819 1104 94-95 4.845915252119617 1104 96-97 4.652695739544022 1104 98-99 4.370592400840836 1104 100-101 3.9063883227704093 1105 1 5.889735089089484 1105 2 6.834290086608988 1105 3 6.709005190557296 1105 4 8.568021031000715 1105 5 8.450441165646506 1105 6 7.570229102635977 1105 7 7.3507318083951105 1105 8 6.6187832783662195 1105 9 6.51007741580171 1105 10-11 6.010868067250673 1105 12-13 5.753579973389307 1105 14-15 6.1099699927243485 1105 16-17 6.743876016255381 1105 18-19 7.203529596980804 1105 20-21 7.647486561170165 1105 22-23 7.4887665063397115 1105 24-25 7.531112666320578 1105 26-27 7.502446514380949 1105 28-29 7.382371429956269 1105 30-31 7.125502608239749 1105 32-33 6.908181237715073 1105 34-35 8.991412249258861 1105 36-37 -3.8105843821241674 1105 38-39 -2.521784391591847 1105 40-41 5.149004437417123 1105 42-43 7.398122427212311 1105 44-45 -3.519678675885787 1105 46-47 -4.464391124055592 1105 48-49 4.610396028447742 1105 50-51 -9.742044872195315 1105 52-53 0.18326089652289923 1105 54-55 4.89752125104857 1105 56-57 5.964496134145758 1105 58-59 6.315012179433111 1105 60-61 6.249323038736385 1105 62-63 6.3820101353688905 1105 64-65 6.274963826701779 1105 66-67 6.255037949132362 1105 68-69 6.184452217960558 1105 70-71 6.131216453794885 1105 72-73 5.9616914058273025 1105 74-75 5.855811299738853 1105 76-77 5.720655247538037 1105 78-79 5.521145520368989 1105 80-81 5.384047705010941 1105 82-83 5.337058815726818 1105 84-85 5.177157559758442 1105 86-87 5.137846763158485 1105 88-89 5.059262698334495 1105 90-91 4.858632339025776 1105 92-93 4.6532494971096146 1105 94-95 4.22141985492938 1105 96-97 3.928417719260855 1105 98-99 3.681056466517118 1105 100-101 3.2131328662082854 1106 1 5.730033254396709 1106 2 7.021417769615141 1106 3 6.662771164751838 1106 4 8.019632961918937 1106 5 7.8629039696348855 1106 6 7.143814324971455 1106 7 6.963208129270658 1106 8 6.237952564553822 1106 9 6.135073753417984 1106 10-11 5.661767333397901 1106 12-13 5.467349590778085 1106 14-15 5.793202841793043 1106 16-17 6.307453687293847 1106 18-19 6.499085438246492 1106 20-21 7.014389100950208 1106 22-23 6.814556928314644 1106 24-25 6.810349183108617 1106 26-27 6.866949291848524 1106 28-29 6.743577453620372 1106 30-31 6.344419711810463 1106 32-33 6.457497282288227 1106 34-35 8.635325021154 1106 36-37 -0.4196277272429647 1106 38-39 0.4816712137180481 1106 40-41 6.18600752449068 1106 42-43 7.287743283207568 1106 44-45 -3.666882912774664 1106 46-47 -4.682155477721386 1106 48-49 4.0259807044912925 1106 50-51 -9.781378428813147 1106 52-53 -0.1008585957551098 1106 54-55 4.4766183257938685 1106 56-57 5.392987307871177 1106 58-59 5.746298582708317 1106 60-61 5.760140971414611 1106 62-63 5.769031494578012 1106 64-65 5.718874150767277 1106 66-67 5.595181334848622 1106 68-69 5.4674533375969006 1106 70-71 5.462587435902513 1106 72-73 5.339621839351505 1106 74-75 5.22490242387115 1106 76-77 4.993018792268682 1106 78-79 4.97856945535014 1106 80-81 4.700112076097048 1106 82-83 4.539987777276989 1106 84-85 4.449335387701385 1106 86-87 4.370480371363271 1106 88-89 4.474303843870091 1106 90-91 4.0842403017453766 1106 92-93 3.990417880548879 1106 94-95 3.795023283875345 1106 96-97 3.422448899649236 1106 98-99 3.135768388795391 1106 100-101 2.864470869545009 1107 1 5.836621765435002 1107 2 7.034240243280106 1107 3 6.407365454183076 1107 4 7.791301419829054 1107 5 8.118370271883695 1107 6 6.681746297931433 1107 7 6.807320815678878 1107 8 6.262647982507396 1107 9 6.462686046599032 1107 10-11 5.753558412001762 1107 12-13 5.56116350107866 1107 14-15 5.70121227356179 1107 16-17 6.569102151324547 1107 18-19 6.955091363947805 1107 20-21 7.414974218579413 1107 22-23 7.319580285673968 1107 24-25 7.169525295760007 1107 26-27 7.2789317182446425 1107 28-29 7.150994718591679 1107 30-31 6.891014163766737 1107 32-33 6.738488677673825 1107 34-35 8.890705729633904 1107 36-37 -0.15267368704061468 1107 38-39 0.6465511667957031 1107 40-41 6.795693223712938 1107 42-43 7.894318456742919 1107 44-45 -3.3873698718822 1107 46-47 -4.38405110355667 1107 48-49 4.5294578740091715 1107 50-51 -9.680133100204802 1107 52-53 0.26637886867650096 1107 54-55 4.9707614890838165 1107 56-57 6.257659892569709 1107 58-59 6.609188639929872 1107 60-61 6.473323814325823 1107 62-63 6.2779953965575395 1107 64-65 6.138336876731962 1107 66-67 6.143859806015119 1107 68-69 6.115494303622615 1107 70-71 6.111570597969628 1107 72-73 5.885559522412745 1107 74-75 5.815714823092222 1107 76-77 5.981907987731713 1107 78-79 5.8813691026350625 1107 80-81 5.714474412602064 1107 82-83 5.581513290613872 1107 84-85 5.413972236142023 1107 86-87 5.172953029825983 1107 88-89 4.88679304954479 1107 90-91 4.79579170679631 1107 92-93 4.605386491354446 1107 94-95 4.524118913633963 1107 96-97 4.4421967456768865 1107 98-99 4.090854920543668 1107 100-101 3.5674368185333236 1108 1 5.9951600835985985 1108 2 6.348432938588626 1108 3 6.6138029730737635 1108 4 7.899876227324185 1108 5 8.16979021078853 1108 6 7.392356396644381 1108 7 7.6573609053599725 1108 8 6.714939102735745 1108 9 4.921852097677899 1108 10-11 5.896118993750658 1108 12-13 5.35330278794752 1108 14-15 6.059917447038796 1108 16-17 6.217693529815779 1108 18-19 6.7618560029949215 1108 20-21 7.738648460265768 1108 22-23 7.848923392743718 1108 24-25 7.186250327676145 1108 26-27 7.601077407351784 1108 28-29 7.4615437381019625 1108 30-31 7.291464263790267 1108 32-33 7.246874323648122 1108 34-35 9.640062325575215 1108 36-37 -3.474118927437175 1108 38-39 -2.1982934119345323 1108 40-41 5.641282445796627 1108 42-43 8.076932058919876 1108 44-45 -2.9900292862776006 1108 46-47 -4.056350244068268 1108 48-49 5.187266335131053 1108 50-51 -9.730051806001653 1108 52-53 0.5601691528680028 1108 54-55 5.660171138311664 1108 56-57 6.8044834008526465 1108 58-59 7.235634136857204 1108 60-61 7.175566550456299 1108 62-63 7.237765973083345 1108 64-65 7.049243269661908 1108 66-67 7.131540297007039 1108 68-69 7.105450196758792 1108 70-71 7.028942810321528 1108 72-73 7.004311795221415 1108 74-75 6.719490693211512 1108 76-77 6.576749573994093 1108 78-79 6.470429898175247 1108 80-81 6.324862913789685 1108 82-83 6.353534251313873 1108 84-85 6.156919130234996 1108 86-87 6.1305883690547205 1108 88-89 6.005779392015393 1108 90-91 6.045263354325188 1108 92-93 5.841584771945792 1108 94-95 5.737599199748626 1108 96-97 5.481640382298439 1108 98-99 5.286664953896421 1108 100-101 4.76351347490418 1109 1 4.8579847560045515 1109 2 6.351944659884385 1109 3 6.11626865737901 1109 4 7.677919763462839 1109 5 7.660944791586722 1109 6 5.103339216898188 1109 7 4.8244356547054394 1109 8 3.278485628164521 1109 9 2.958479813460574 1109 10-11 2.611256835463866 1109 12-13 3.2951801207346634 1109 14-15 3.8788249390129437 1109 16-17 3.0591760270890376 1109 18-19 4.037458043010375 1109 20-21 4.473436023541595 1109 22-23 6.000865161984816 1109 24-25 6.0519998523148395 1109 26-27 4.78192973559975 1109 28-29 4.56074290071621 1109 30-31 4.539883773455305 1109 32-33 4.356615698044116 1109 34-35 6.502125945240529 1109 36-37 -4.612672262234744 1109 38-39 -3.4056461110599656 1109 40-41 2.985505591149188 1109 42-43 3.8685860381778774 1109 44-45 -4.650142633680831 1109 46-47 -6.117269127453566 1109 48-49 1.9057952448989681 1109 50-51 -10.064594500492255 1109 52-53 -1.2959706875226011 1109 54-55 2.3557132204025635 1109 56-57 3.7831470989653404 1109 58-59 4.585199322521522 1109 60-61 4.920101040142949 1109 62-63 4.772752680131095 1109 64-65 4.459833824246342 1109 66-67 4.651994005932739 1109 68-69 4.685199711712627 1109 70-71 4.48284008740346 1109 72-73 4.449282961901577 1109 74-75 4.664916616892018 1109 76-77 4.801602770427589 1109 78-79 4.796221672783773 1109 80-81 4.634207899258316 1109 82-83 4.234890283470106 1109 84-85 4.266174823195726 1109 86-87 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-14.56034365175542 2313 76-77 -14.053770572184307 2313 78-79 -13.56175112425921 2313 80-81 -13.021063771443576 2313 82-83 -12.538130223733337 2313 84-85 -12.017098245532077 2313 86-87 -11.455435921614372 2313 88-89 -10.898532982458638 2313 90-91 -10.332842609017778 2313 92-93 -9.732411726745893 2313 94-95 -9.146323488588735 2313 96-97 -8.413585306162867 2313 98-99 -7.588669576442394 2313 100-101 -6.552216855678752 2314 1 -20.66198904884155 2314 2 -19.54131008798726 2314 3 -19.964936319700232 2314 4 -21.878072377990993 2314 5 -21.866669266479203 2314 6 -22.607204332545166 2314 7 -22.686588139226018 2314 8 -23.47768114764862 2314 9 -24.62130625944927 2314 10-11 -24.870079117384858 2314 12-13 -24.81669501569843 2314 14-15 -25.01453895533327 2314 16-17 -24.145473612727596 2314 18-19 -23.522681664477073 2314 20-21 -23.02700056235668 2314 22-23 -22.935175003917823 2314 24-25 -22.65308637340007 2314 26-27 -22.310189888936378 2314 28-29 -22.237336121331378 2314 30-31 -22.154615244226424 2314 32-33 -21.870081529635428 2314 34-35 -19.08802904275336 2314 36-37 -17.446005595568078 2314 38-39 -16.55124750813484 2314 40-41 -17.86278080920008 2314 42-43 -17.795679406048276 2314 44-45 -15.864288016784958 2314 46-47 -16.630257557927262 2314 48-49 -16.113414534625154 2314 50-51 -14.142197643910723 2314 52-53 -16.956852591037116 2314 54-55 -18.5798903596018 2314 56-57 -18.775573704352713 2314 58-59 -18.412290308563396 2314 60-61 -18.08470140473808 2314 62-63 -17.609587420283663 2314 64-65 -17.11449492493506 2314 66-67 -16.700658253399286 2314 68-69 -16.23981666025856 2314 70-71 -15.656649107095557 2314 72-73 -15.195226970427608 2314 74-75 -14.655646578916755 2314 76-77 -14.099947109511287 2314 78-79 -13.60270869176545 2314 80-81 -13.099802142217344 2314 82-83 -12.62889491626794 2314 84-85 -12.110926246439725 2314 86-87 -11.550171569447365 2314 88-89 -10.992134071634949 2314 90-91 -10.425195683671737 2314 92-93 -9.794698996997765 2314 94-95 -9.146323488588735 2314 96-97 -8.427540377640062 2314 98-99 -7.623613983068216 2314 100-101 -6.593174423184992 2315 1 -20.66198904884155 2315 2 -19.54131008798726 2315 3 -19.964936319700232 2315 4 -16.896640610653186 2315 5 -21.866669266479203 2315 6 -22.607204332545166 2315 7 -22.686588139226018 2315 8 -23.47768114764862 2315 9 -19.985064648711017 2315 10-11 -18.47444153349224 2315 12-13 -15.031348259546302 2315 14-15 -14.90503112535564 2315 16-17 -20.09234162167614 2315 18-19 -23.522681664477073 2315 20-21 -23.02700056235668 2315 22-23 -22.935175003917823 2315 24-25 -22.65308637340007 2315 26-27 -22.310189888936378 2315 28-29 -22.237336121331378 2315 30-31 -22.154615244226424 2315 32-33 -21.870081529635428 2315 34-35 -19.08802904275336 2315 36-37 -17.446005595568078 2315 38-39 -16.55124750813484 2315 40-41 -17.86278080920008 2315 42-43 -17.795679406048276 2315 44-45 -15.864288016784958 2315 46-47 -16.630257557927262 2315 48-49 -16.113414534625154 2315 50-51 -14.142197643910723 2315 52-53 -16.956852591037116 2315 54-55 -18.5798903596018 2315 56-57 -18.775573704352713 2315 58-59 -18.412290308563396 2315 60-61 -18.08470140473808 2315 62-63 -17.609587420283663 2315 64-65 -17.11449492493506 2315 66-67 -16.700658253399286 2315 68-69 -16.23981666025856 2315 70-71 -15.656649107095557 2315 72-73 -15.195226970427608 2315 74-75 -14.655646578916755 2315 76-77 -14.099947109511287 2315 78-79 -13.60270869176545 2315 80-81 -13.099802142217344 2315 82-83 -12.62889491626794 2315 84-85 -12.110926246439725 2315 86-87 -11.550171569447365 2315 88-89 -10.992134071634949 2315 90-91 -10.425195683671737 2315 92-93 -9.794698996997765 2315 94-95 -9.146323488588735 2315 96-97 -8.427540377640062 2315 98-99 -7.623613983068216 2315 100-101 -6.593174423184992 2316 1 -20.66198904884155 2316 2 -19.54131008798726 2316 3 -19.964936319700232 2316 4 -21.878072377990993 2316 5 -21.866669266479203 2316 6 -22.607204332545166 2316 7 -22.686588139226018 2316 8 -23.47768114764862 2316 9 -24.62130625944927 2316 10-11 -24.870079117384858 2316 12-13 -24.81669501569843 2316 14-15 -25.01453895533327 2316 16-17 -24.145473612727596 2316 18-19 -23.522681664477073 2316 20-21 -23.02700056235668 2316 22-23 -22.935175003917823 2316 24-25 -22.65308637340007 2316 26-27 -22.310189888936378 2316 28-29 -22.237336121331378 2316 30-31 -22.154615244226424 2316 32-33 -21.870081529635428 2316 34-35 -19.08802904275336 2316 36-37 -17.446005595568078 2316 38-39 -16.55124750813484 2316 40-41 -17.86278080920008 2316 42-43 -17.795679406048276 2316 44-45 -15.864288016784958 2316 46-47 -16.630257557927262 2316 48-49 -16.113414534625154 2316 50-51 -14.142197643910723 2316 52-53 -16.956852591037116 2316 54-55 -18.5798903596018 2316 56-57 -18.775573704352713 2316 58-59 -18.412290308563396 2316 60-61 -18.08470140473808 2316 62-63 -17.609587420283663 2316 64-65 -17.11449492493506 2316 66-67 -16.700658253399286 2316 68-69 -16.23981666025856 2316 70-71 -15.656649107095557 2316 72-73 -15.195226970427608 2316 74-75 -14.655646578916755 2316 76-77 -14.099947109511287 2316 78-79 -13.60270869176545 2316 80-81 -13.099802142217344 2316 82-83 -12.62889491626794 2316 84-85 -12.110926246439725 2316 86-87 -11.550171569447365 2316 88-89 -10.992134071634949 2316 90-91 -10.425195683671737 2316 92-93 -9.794698996997765 2316 94-95 -9.146323488588735 2316 96-97 -8.427540377640062 2316 98-99 -7.623613983068216 2316 100-101 -6.593174423184992 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 694002.0 3 42733.0 4 89938.0 5 54788.0 6 47879.0 7 58909.0 8 76127.0 9 90025.0 10 93520.0 11 87367.0 12 76820.0 13 70624.0 14 68339.0 15 73450.0 16 79534.0 17 85498.0 18 88301.0 19 92590.0 20 97501.0 21 103375.0 22 108965.0 23 116181.0 24 123895.0 25 129989.0 26 137750.0 27 146134.0 28 155206.0 29 163891.0 30 169325.0 31 168650.0 32 163578.0 33 157864.0 34 146609.0 35 122765.0 36 84977.0 37 35782.0 38 4554.0 39 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.40072467846181 19.879875021103988 13.769269563937506 23.950130736496693 2 15.376555252499818 20.874536101155247 40.52425570269055 23.22465294365438 3 18.44986956056671 26.017702674057258 30.1898933387775 25.34253442659853 4 12.727226692005921 17.056813747538808 38.775345460827914 31.440614099627357 5 13.295086026712472 37.93671447134083 35.320552323688865 13.447647178257835 6 30.466830805843887 37.400552024226805 18.34517464485724 13.787442525072066 7 26.722816764047685 32.787914377269445 22.9887137433495 17.500555115333373 8 25.15616082353511 35.83503920342185 21.06599505929478 17.942804913748258 9 26.37543197346361 16.2210804724672 21.09025593209271 36.313231621976485 10-11 24.70890571855072 26.406555915514296 28.907146595489213 19.97739177044577 12-13 25.08429437074897 24.208063512965268 28.83546453285709 21.872177583428666 14-15 22.460927354250433 25.593410663548728 26.387349451353852 25.558312530846987 16-17 22.251699793069807 28.5102556612786 27.05061228131152 22.187432264340075 18-19 22.14537188591565 27.84807228961357 28.731225825397267 21.275329999073517 20-21 22.889331194805695 27.046096556536845 28.594211114918462 21.470361133738994 22-23 22.740369492232244 26.79611400179062 28.327804252601315 22.13571225337582 24-25 22.557330099631173 27.014292385252574 28.2696733565864 22.158704158529847 26-27 22.349462381323924 27.29933438447789 28.214625558348498 22.136577675849683 28-29 22.28004194516165 27.508888873519165 28.204623118679162 22.006446062640023 30-31 22.731486772750404 27.26001859552109 28.024683183812144 21.983811447916356 32-33 22.552122956304487 27.384704703134883 27.912234339219694 22.150938001340936 34-35 22.777129344516766 27.327241130657004 27.982511055854705 21.913118468971525 36-37 23.193727099391566 27.40932415631658 27.55903800037763 21.837910743914225 38-39 23.07511792422731 27.36059777602647 27.659377687986993 21.904906611759227 40-41 22.98224151070882 27.2049107301748 27.749800433087035 22.063047326029345 42-43 23.01526606056663 27.248322678058763 27.49087653434331 22.2455347270313 44-45 23.272606481518512 27.449706150414222 27.20767508767344 22.070012280393826 46-47 22.65883120066039 27.28260449849731 27.54750064389548 22.51106365694682 48-49 23.12355341318976 27.37045013501354 27.17949957102425 22.326496880772442 50-51 23.363710405021585 27.418564383574513 26.985313532975898 22.232411678428 52-53 22.407607559975553 27.504476040433197 27.525255094520563 22.562661305070687 54-55 22.200115463948194 27.32306783487035 27.692661372289155 22.784155328892297 56-57 22.282579117259935 27.509459514440653 27.486553777755702 22.721407590543706 58-59 22.39818439282719 27.485494421486305 27.590893586923716 22.525427598762786 60-61 22.496471548215894 27.562670002094375 27.505320217783186 22.43553823190655 62-63 22.402641640653183 27.38769159861635 27.531812206191574 22.677854554538897 64-65 22.164366546597154 27.255130369977476 27.600165165518003 22.980337917907367 66-67 22.330767225118322 27.33045563473333 27.4489653815836 22.88981175856475 68-69 22.205524969102527 27.351566840374968 27.53813798346945 22.904770207053048 70-71 22.214404806940323 27.179608412250577 27.446202691985288 23.159784088823805 72-73 22.305070485749205 27.315244506198372 27.34099558139699 23.038689426655438 74-75 22.23693485782385 27.462313419402033 27.292631843680066 23.00811987909405 76-77 22.206398411307944 27.180940811406508 27.343923743616678 23.26873703366887 78-79 22.140485908363754 27.242383433534435 27.319335466941762 23.29779519116005 80-81 22.255442796085354 27.423880804371276 27.197112378945214 23.123564020598153 82-83 22.409135384193682 27.206555539897682 27.311956083235874 23.07235299267276 84-85 22.383795964988476 27.21999566194272 27.11752065085285 23.278687722215956 86-87 22.090245195613388 27.316522549043636 27.23198701715866 23.36124523818431 88-89 22.227283810041882 27.098068547874128 27.142561608017974 23.532086034066012 90-91 22.30419139697514 27.047032299259143 27.192119607815645 23.45665669595007 92-93 22.417209344023217 26.86821045213789 27.14681581216049 23.567764391678402 94-95 22.17319045043125 26.842120984875095 27.234138913893936 23.75054965079972 96-97 22.149393277469972 26.755089512618973 27.133388088099352 23.9621291218117 98-99 21.950741607653303 26.704121781911084 27.140284585959595 24.20485202447602 100-101 22.29677842960933 26.87760575826349 26.910759632465624 23.91485617966156 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 49768.0 1 53585.0 2 39617.5 3 23534.0 4 30799.0 5 39175.0 6 41648.0 7 39675.0 8 36394.5 9 33203.5 10 29591.0 11 25588.0 12 21732.0 13 18308.5 14 15503.5 15 13125.5 16 11379.5 17 10299.5 18 9858.0 19 9988.0 20 10510.5 21 11579.0 22 13279.0 23 15446.5 24 18140.0 25 21564.5 26 26154.0 27 32405.0 28 40159.0 29 50050.0 30 61134.0 31 72033.0 32 82672.5 33 92357.0 34 100942.5 35 108285.0 36 115134.0 37 121168.5 38 124928.0 39 126153.5 40 126058.5 41 126362.0 42 127608.0 43 129901.5 44 131936.0 45 133959.0 46 135477.5 47 136068.0 48 135991.0 49 134418.0 50 131533.5 51 126927.5 52 121682.0 53 115537.5 54 107611.0 55 98553.0 56 88444.5 57 77861.5 58 67716.5 59 58076.5 60 48619.0 61 39885.0 62 32361.0 63 25705.5 64 19948.0 65 15090.0 66 11167.5 67 8382.0 68 6310.5 69 4623.0 70 3412.0 71 2489.0 72 1766.5 73 1293.0 74 923.5 75 624.0 76 406.0 77 264.5 78 177.0 79 107.0 80 72.5 81 50.5 82 26.0 83 11.5 84 8.0 85 5.0 86 5.0 87 5.5 88 4.0 89 2.5 90 1.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.5 96 2.0 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.19574412762974 2 21.07998681357459 3 16.874701099952176 4 22.062886248508402 5 21.90532239417197 6 15.872602415344542 7 14.684941937940224 8 11.812344165703221 9 2.9469107083989172 10-11 6.360430508931017 12-13 3.4927892539919116 14-15 7.265198051752077 16-17 11.263169013757528 18-19 14.302767756404005 20-21 14.70195892707071 22-23 12.654336894596815 24-25 15.16047740431716 26-27 12.73100620783478 28-29 12.728289989924457 30-31 13.569957701290319 32-33 12.067564179353958 34-35 18.917262145208547 36-37 23.645466237643532 38-39 22.6873726047369 40-41 17.813142780199932 42-43 17.749102719468084 44-45 23.086819146318245 46-47 12.521985114197228 48-49 21.216946878206645 50-51 20.914610930803768 52-53 11.010502709253249 54-55 12.59787670692241 56-57 12.127379012225301 58-59 13.11725471623648 60-61 12.541660282393801 62-63 12.507173601147775 64-65 13.556074809748669 66-67 11.895954924712012 68-69 12.915825103425222 70-71 11.568325648990356 72-73 10.928180412586535 74-75 11.013404651465132 76-77 11.059510709327538 78-79 11.469531928328992 80-81 11.434929169394492 82-83 9.968670631880505 84-85 9.879093479684082 86-87 10.019245680749211 88-89 11.401951498098647 90-91 8.921847213903321 92-93 10.918545964443084 94-95 11.012162620198447 96-97 10.58026075691939 98-99 10.427328402353126 100-101 10.544845454165564 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4307460.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.07427703066338 #Duplication Level Percentage of deduplicated Percentage of total 1 98.5110913491076 94.64381880867151 2 1.02194123369043 1.963645303892646 3 0.2100734982277141 0.6054797839658993 4 0.07930325743289449 0.30476012496167676 5 0.05078652252312172 0.2439639217155211 6 0.028422331108260902 0.16383929476513795 7 0.017693825545246383 0.11899450480163606 8 0.012584888842372143 0.09672672776337327 9 0.009932446774189734 0.08588273786782409 >10 0.05251272615595437 0.9229798940111155 >50 0.0035820953643005125 0.23907385848887389 >100 0.0019792029251222616 0.3294697981436411 >500 2.415557571984884E-5 0.019819029704065107 >1k 4.8311151361881964E-5 0.07997233752951059 >5k 2.4155575680940972E-5 0.1815738737176119 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 7824 0.18163836692621638 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.643107539013711E-5 2 0.0 0.0 0.0 0.0 4.643107539013711E-5 3 0.0 0.0 0.0 0.0 4.643107539013711E-5 4 0.0 0.0 0.0 2.3215537695068555E-5 4.643107539013711E-5 5 0.0 0.0 0.0 2.3215537695068555E-5 4.643107539013711E-5 6 9.286215078027422E-5 0.0 0.0 2.3215537695068555E-5 6.964661308520567E-5 7 9.286215078027422E-5 0.0 0.0 2.3215537695068555E-5 6.964661308520567E-5 8 9.286215078027422E-5 0.0 0.0 2.3215537695068555E-5 6.964661308520567E-5 9 9.286215078027422E-5 0.0 0.0 4.643107539013711E-5 9.286215078027422E-5 10-11 9.286215078027422E-5 0.0 0.0 9.286215078027422E-5 9.286215078027422E-5 12-13 9.286215078027422E-5 0.0 0.0 1.1607768847534278E-4 1.2768545732287705E-4 14-15 9.286215078027422E-5 0.0 0.0 1.2768545732287705E-4 1.6250876386547989E-4 16-17 1.3929322617041133E-4 0.0 0.0 1.3929322617041133E-4 1.8572430156054844E-4 18-19 1.3929322617041133E-4 0.0 0.0 1.3929322617041133E-4 1.8572430156054844E-4 20-21 1.3929322617041133E-4 0.0 0.0 1.6250876386547989E-4 1.8572430156054844E-4 22-23 1.3929322617041133E-4 0.0 0.0 2.9019422118835693E-4 2.08939839255617E-4 24-25 1.6250876386547989E-4 0.0 0.0 3.598408342735626E-4 2.7858645234082267E-4 26-27 1.6250876386547989E-4 0.0 0.0 5.107418292915082E-4 2.7858645234082267E-4 28-29 1.6250876386547989E-4 0.0 2.3215537695068555E-5 0.0011491691159058936 2.7858645234082267E-4 30-31 1.6250876386547989E-4 0.0 2.3215537695068555E-5 0.003876994795076449 2.7858645234082267E-4 32-33 1.6250876386547989E-4 0.0 2.3215537695068555E-5 0.011538122234449073 2.7858645234082267E-4 34-35 1.6250876386547989E-4 0.0 2.3215537695068555E-5 0.022507463795368964 2.7858645234082267E-4 36-37 1.6250876386547989E-4 0.0 2.3215537695068555E-5 0.03924586647351339 3.0180199003589125E-4 38-39 1.6250876386547989E-4 0.0 2.3215537695068555E-5 0.06330877129445195 3.0180199003589125E-4 40-41 1.6250876386547989E-4 0.0 2.3215537695068555E-5 0.09064506693039517 3.0180199003589125E-4 42-43 1.6250876386547989E-4 0.0 4.643107539013711E-5 0.11910731614454922 3.4823306542602835E-4 44-45 1.6250876386547989E-4 0.0 4.643107539013711E-5 0.15605484438625083 3.4823306542602835E-4 46-47 1.6250876386547989E-4 0.0 4.643107539013711E-5 0.19982774071030257 3.714486031210969E-4 48-49 1.6250876386547989E-4 0.0 4.643107539013711E-5 0.24856876210109902 3.714486031210969E-4 50-51 1.6250876386547989E-4 0.0 4.643107539013711E-5 0.3036940563580393 3.714486031210969E-4 52-53 1.6250876386547989E-4 0.0 4.643107539013711E-5 0.37603367181587294 3.714486031210969E-4 54-55 1.6250876386547989E-4 0.0 4.643107539013711E-5 0.4588202792364874 3.9466414081616545E-4 56-57 1.6250876386547989E-4 0.0 4.643107539013711E-5 0.5547584887613582 4.062719096636997E-4 58-59 1.6250876386547989E-4 0.0 4.643107539013711E-5 0.6620026651437274 4.294874473587683E-4 60-61 1.8572430156054844E-4 0.0 4.643107539013711E-5 0.7714058865317379 4.4109521620630255E-4 62-63 2.08939839255617E-4 0.0 4.643107539013711E-5 0.8856843708357129 4.4109521620630255E-4 64-65 2.08939839255617E-4 0.0 4.643107539013711E-5 1.013543944691303 4.4109521620630255E-4 66-67 2.7858645234082267E-4 0.0 4.643107539013711E-5 1.154090809897248 4.4109521620630255E-4 68-69 2.7858645234082267E-4 0.0 4.643107539013711E-5 1.3060597196491668 4.6431075390137113E-4 70-71 3.0180199003589125E-4 0.0 4.643107539013711E-5 1.4692417341078037 5.107418292915082E-4 72-73 3.4823306542602835E-4 0.0 4.643107539013711E-5 1.6393535865684186 5.339573669865768E-4 74-75 3.4823306542602835E-4 0.0 4.643107539013711E-5 1.816499746950639 5.339573669865768E-4 76-77 3.4823306542602835E-4 0.0 4.643107539013711E-5 2.021028634044193 5.339573669865768E-4 78-79 3.4823306542602835E-4 0.0 4.643107539013711E-5 2.243352230781017 5.339573669865768E-4 80-81 3.4823306542602835E-4 0.0 4.643107539013711E-5 2.480092676426479 5.571729046816453E-4 82-83 3.4823306542602835E-4 0.0 4.643107539013711E-5 2.7265023935219364 5.571729046816453E-4 84-85 3.4823306542602835E-4 0.0 4.643107539013711E-5 2.9801437506094075 5.571729046816453E-4 86-87 3.7144860312109693E-4 0.0 4.643107539013711E-5 3.250894958978145 5.571729046816453E-4 88-89 3.9466414081616545E-4 0.0 4.643107539013711E-5 3.5424356813528157 5.571729046816453E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3340 0.0 51.230442 1 GTATCAA 6185 0.0 44.02032 1 ATCAACG 7535 0.0 35.11202 3 TCAACGC 7675 0.0 34.980106 4 CAACGCA 7735 0.0 33.71458 5 TATCAAC 7840 0.0 33.69025 2 AACGCAG 8495 0.0 30.965435 6 GTACATG 9620 0.0 27.255758 1 ACGCAGA 9810 0.0 26.550663 7 TACATGG 10010 0.0 26.135468 2 CGCAGAG 10130 0.0 25.546438 8 ACATGGG 10635 0.0 24.309662 3 GCAGAGT 11415 0.0 21.573357 9 CATGGGG 7900 0.0 20.746685 4 GTGGTAT 1620 0.0 19.26071 1 GAGTACA 7405 0.0 19.029554 1 AGAGTAC 10010 0.0 18.68049 10-11 AGTACAT 7490 0.0 18.270401 2 CAGAGTA 10765 0.0 18.172049 10-11 GAGTACT 5860 0.0 18.04346 12-13 >>END_MODULE