FastQCFastQC Report
Sat 18 Jun 2016
SRR1930957_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930957_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4307460
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57730.13402329911363078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45920.1066057490957548No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49450.052.9042241
GTATCAA86200.041.1451231
ATCAACG109700.031.8482383
CAACGCA109300.031.7362175
TCAACGC111000.031.4317474
AACGCAG116450.029.9095216
TATCAAC117850.029.687412
ACGCAGA134550.025.6038827
GTACATG133300.025.5384221
CGCAGAG137600.024.7949568
TACATGG139900.023.9903242
ACATGGG138400.023.666133
CATGGGG98050.021.2046284
GCAGAGT160300.020.721329
AGAGTAC147550.018.74917810-11
GAGTACA104450.018.5462781
GTGGTAT23400.018.26131
CAGAGTA156450.018.1830410-11
GAGTACT86650.018.01690512-13
AGTACAT104550.017.4811942
Produced by FastQC (version 0.11.5)