##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930957_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4307460 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.2470750744058 31.0 31.0 33.0 30.0 34.0 2 31.368723098995694 31.0 31.0 34.0 30.0 34.0 3 31.45477497179312 31.0 31.0 34.0 30.0 34.0 4 35.32080553272694 37.0 35.0 37.0 33.0 37.0 5 35.105178922149015 37.0 35.0 37.0 32.0 37.0 6 35.13761474279506 37.0 35.0 37.0 32.0 37.0 7 35.01802941872937 36.0 35.0 37.0 32.0 37.0 8 34.997145185329636 36.0 35.0 37.0 32.0 37.0 9 36.49795262173067 38.0 35.0 39.0 32.0 39.0 10-11 36.42548532081552 38.0 35.0 39.0 32.0 39.0 12-13 36.31881886308869 38.0 35.0 39.0 32.0 39.0 14-15 37.336151699609516 39.0 36.0 41.0 32.0 41.0 16-17 37.301878136999534 39.0 36.0 41.0 32.0 41.0 18-19 37.290102635892154 39.0 36.0 40.0 31.5 41.0 20-21 37.254233353298694 39.0 36.0 40.0 31.0 41.0 22-23 37.12968768137139 39.0 36.0 40.0 31.0 41.0 24-25 37.00599587692051 39.0 36.0 40.0 31.0 41.0 26-27 36.732777205127846 39.0 35.5 40.0 30.0 41.0 28-29 36.55374965757082 38.0 35.0 40.0 30.0 41.0 30-31 36.324489142093014 38.0 35.0 40.0 30.0 41.0 32-33 36.099085423892504 38.0 35.0 40.0 30.0 41.0 34-35 36.300282184860684 38.0 35.0 40.0 30.0 41.0 36-37 36.36297586048391 38.0 35.0 40.0 30.0 41.0 38-39 36.335007057523455 38.0 35.0 40.0 30.0 41.0 40-41 36.245912672433406 38.0 35.0 40.0 30.0 41.0 42-43 36.12011150422755 38.0 35.0 40.0 30.0 41.0 44-45 35.94208756436508 38.0 35.0 40.0 29.0 41.0 46-47 35.738250616372525 38.0 34.0 40.0 28.0 41.0 48-49 35.55295231992868 38.0 34.0 40.0 28.0 41.0 50-51 35.29209580588096 38.0 34.0 40.0 27.0 41.0 52-53 35.0869616897197 38.0 34.0 40.0 26.5 41.0 54-55 34.921041518667614 38.0 33.0 40.0 26.0 41.0 56-57 34.6686212988629 37.5 33.0 40.0 26.0 41.0 58-59 34.42897786630636 37.0 33.0 40.0 26.0 41.0 60-61 34.122126380744106 37.0 33.0 40.0 25.5 41.0 62-63 33.810861969699076 36.0 32.0 39.0 25.0 41.0 64-65 33.50566888607207 36.0 32.0 39.0 24.5 40.5 66-67 33.17414067687221 35.5 31.5 39.0 23.0 40.0 68-69 32.80800715502872 35.0 31.0 38.5 22.5 40.0 70-71 32.4126475927809 35.0 31.0 38.0 22.0 40.0 72-73 31.99697072056386 35.0 31.0 37.0 22.0 39.5 74-75 31.47265732009119 34.0 30.0 37.0 20.0 39.0 76-77 30.085215184818892 33.0 28.5 35.5 19.5 38.0 78-79 30.867778690922258 34.0 30.0 36.0 20.0 38.5 80-81 30.87163560427723 34.0 30.0 35.5 20.0 37.5 82-83 30.600300409057773 34.0 30.0 35.0 20.0 37.0 84-85 30.285580016993773 34.0 30.0 35.0 18.5 36.5 86-87 29.948695518936916 34.0 29.5 35.0 18.0 36.0 88-89 29.612583750052234 34.0 29.0 35.0 14.5 36.0 90-91 29.284779080943295 34.0 29.0 35.0 8.5 35.5 92-93 28.93021885287385 34.0 29.0 35.0 4.5 35.0 94-95 28.521572341937013 33.0 29.0 35.0 2.0 35.0 96-97 28.07326510286805 33.0 28.5 35.0 2.0 35.0 98-99 27.59606624321526 33.0 27.0 35.0 2.0 35.0 100-101 26.392094180793322 32.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5744661853935398 1101 2 0.6296790678118924 1101 3 0.6066132675704026 1101 4 0.3858027740111396 1101 5 0.5607883706430741 1101 6 0.5335646760666108 1101 7 0.5396901410839732 1101 8 0.515830098128454 1101 9 0.7927188937531966 1101 10-11 0.7508138056433253 1101 12-13 0.7638737491852581 1101 14-15 0.8407275471800304 1101 16-17 0.9410281460805194 1101 18-19 0.8627856604802844 1101 20-21 0.8575166233237539 1101 22-23 0.8862323690157652 1101 24-25 0.9370779480081168 1101 26-27 0.7782593884134386 1101 28-29 0.8120029322130691 1101 30-31 0.8911424430465047 1101 32-33 0.8266012226727852 1101 34-35 0.8158068061180686 1101 36-37 0.951418981630944 1101 38-39 1.0025443909003044 1101 40-41 1.0166149289141586 1101 42-43 0.9047264513985667 1101 44-45 0.9329421369645843 1101 46-47 0.9734244327103809 1101 48-49 1.08065842140644 1101 50-51 1.0545018827311807 1101 52-53 1.0241555210424664 1101 54-55 0.9463441446620493 1101 56-57 1.1501774776550704 1101 58-59 1.0164655756505212 1101 60-61 1.0066553062598587 1101 62-63 0.9894494939550142 1101 64-65 1.0232032279217478 1101 66-67 1.0748921441439308 1101 68-69 0.9469265673298821 1101 70-71 0.9051467605381944 1101 72-73 0.9362276230352649 1101 74-75 0.9959976059605502 1101 76-77 1.1286093777433237 1101 78-79 1.1260185692423441 1101 80-81 0.9870363042495995 1101 82-83 1.0462575256285582 1101 84-85 1.1385266655434272 1101 86-87 1.097758412916832 1101 88-89 1.0999128425888784 1101 90-91 0.970927152519323 1101 92-93 1.014930031789202 1101 94-95 1.1291118845871466 1101 96-97 1.2825868576849757 1101 98-99 1.207997590435678 1101 100-101 1.4197467155779755 1102 1 0.5221184673566164 1102 2 0.5511965730557691 1102 3 0.5570029221231678 1102 4 0.3569425105561521 1102 5 0.4453205232880535 1102 6 0.4169616289365834 1102 7 0.4709548523463667 1102 8 0.424703472284655 1102 9 0.6147747677421975 1102 10-11 0.5912166031301282 1102 12-13 0.6074826046701958 1102 14-15 0.6205641630235945 1102 16-17 0.6324900134408367 1102 18-19 0.6959116964633907 1102 20-21 0.7083007069163543 1102 22-23 0.8558290598328782 1102 24-25 0.7203954066755642 1102 26-27 0.7764370251188879 1102 28-29 0.8111900606794009 1102 30-31 0.8700544200387128 1102 32-33 0.7623091280146497 1102 34-35 0.7450754997458873 1102 36-37 0.798906553862686 1102 38-39 0.9563153694324313 1102 40-41 0.8105621686159665 1102 42-43 0.8539038495811226 1102 44-45 0.9177522171356713 1102 46-47 0.86184587849759 1102 48-49 0.7548537692429349 1102 50-51 0.7807077126820801 1102 52-53 0.7865421924473495 1102 54-55 0.7510516076084031 1102 56-57 0.7251415582016776 1102 58-59 0.8628120051802952 1102 60-61 0.8020437561108267 1102 62-63 0.7211202985104777 1102 64-65 0.716771107429004 1102 66-67 0.7017853189239958 1102 68-69 0.698719115262108 1102 70-71 0.7118562847736456 1102 72-73 0.6006316461752093 1102 74-75 0.6968118272437174 1102 76-77 0.8462822725974348 1102 78-79 0.8410042538393263 1102 80-81 0.764815357909999 1102 82-83 0.9012186810463696 1102 84-85 0.9625881262866685 1102 86-87 0.952503004047486 1102 88-89 0.9792939884818921 1102 90-91 0.8942430478948005 1102 92-93 1.0886762152213727 1102 94-95 1.056138986751499 1102 96-97 1.0560667576987548 1102 98-99 1.099520841180457 1102 100-101 1.3969119756847803 1103 1 0.4527747067935941 1103 2 0.5156465573826488 1103 3 0.46348062421188274 1103 4 0.30398498362620785 1103 5 0.3733896085856756 1103 6 0.4626834806342046 1103 7 0.47837973373855647 1103 8 0.46901548561839235 1103 9 0.5739797018744923 1103 10-11 0.5272956633844288 1103 12-13 0.6401588104560076 1103 14-15 0.746454055731526 1103 16-17 0.6981975207174997 1103 18-19 0.6905086526897435 1103 20-21 0.727552829718384 1103 22-23 0.681778229216853 1103 24-25 0.7628087743135339 1103 26-27 0.841548325073127 1103 28-29 0.696059226253503 1103 30-31 0.8822374502164507 1103 32-33 0.9534963090448443 1103 34-35 0.8393128652595081 1103 36-37 0.959857308380414 1103 38-39 0.8065748323641628 1103 40-41 0.8915567860918046 1103 42-43 0.8348249924932603 1103 44-45 0.884874132040629 1103 46-47 0.9157478768354324 1103 48-49 0.9298179329427967 1103 50-51 0.8863143783230143 1103 52-53 0.7907525245492124 1103 54-55 0.9034155856070214 1103 56-57 1.0177684387039818 1103 58-59 0.9829429168332027 1103 60-61 1.0009175487705733 1103 62-63 0.8089144071593708 1103 64-65 0.8016934606269075 1103 66-67 0.7998255945887607 1103 68-69 0.7270551139171744 1103 70-71 0.7387471978681148 1103 72-73 0.8201168064123294 1103 74-75 0.9739530845429094 1103 76-77 1.0813425849073965 1103 78-79 1.080252150938108 1103 80-81 0.9989358522341121 1103 82-83 0.9301489068442699 1103 84-85 1.009277444227667 1103 86-87 1.1531009390766052 1103 88-89 1.1051891039134887 1103 90-91 1.1649703054827008 1103 92-93 1.0622337632996377 1103 94-95 1.165267129519279 1103 96-97 1.2372062599647755 1103 98-99 1.421111214497774 1103 100-101 1.648624187472116 1104 1 0.4042729340883824 1104 2 0.49291859983572195 1104 3 0.42469527971179133 1104 4 0.22921861128166654 1104 5 0.3742716254691274 1104 6 0.4051415072009945 1104 7 0.3576341560066041 1104 8 0.4107589171916288 1104 9 0.5635652464669931 1104 10-11 0.4620223931708196 1104 12-13 0.5120923036604452 1104 14-15 0.5941092675953072 1104 16-17 0.5209620000707886 1104 18-19 0.629947635307218 1104 20-21 0.6243078291947057 1104 22-23 0.6225530989974004 1104 24-25 0.6471336673422812 1104 26-27 0.7604213257839874 1104 28-29 0.6584059657073524 1104 30-31 0.6917063982518172 1104 32-33 0.8443895077340287 1104 34-35 0.7026898451953372 1104 36-37 0.7504619610618661 1104 38-39 0.5542818645970442 1104 40-41 0.44062937249162104 1104 42-43 0.36516856294526434 1104 44-45 0.5216466013323213 1104 46-47 0.5619425022633493 1104 48-49 0.5894984739557785 1104 50-51 0.5361679665238839 1104 52-53 0.4950185418488928 1104 54-55 0.6156660913848668 1104 56-57 0.7231902289923653 1104 58-59 0.5728551327307017 1104 60-61 0.5814798625100934 1104 62-63 0.47827916122794534 1104 64-65 0.5732008031134797 1104 66-67 0.4711229669661421 1104 68-69 0.4683587675220622 1104 70-71 0.4288120021535775 1104 72-73 0.31655880058919195 1104 74-75 0.5347139085182668 1104 76-77 0.6506794207945106 1104 78-79 0.7470071872317412 1104 80-81 0.6451844683148842 1104 82-83 0.7242376642802775 1104 84-85 0.8098858454929818 1104 86-87 0.8822986239264061 1104 88-89 0.9943771038720222 1104 90-91 0.840653588804237 1104 92-93 0.8264578660253683 1104 94-95 0.7592141838968054 1104 96-97 0.8021006993974353 1104 98-99 0.9985923308491067 1104 100-101 1.1884024610133253 1105 1 0.157197392583317 1105 2 0.24875372738660317 1105 3 0.2581837482099374 1105 4 0.19312454001517665 1105 5 0.12095982211015865 1105 6 0.2678312360443229 1105 7 0.24635624622980146 1105 8 0.2053604043134314 1105 9 0.34744513463225957 1105 10-11 0.3107868880660547 1105 12-13 0.2760628184998879 1105 14-15 0.3832368722962727 1105 16-17 0.2632485982438624 1105 18-19 0.35089419393084853 1105 20-21 0.4009746956253295 1105 22-23 0.47997177900168 1105 24-25 0.542473113707274 1105 26-27 0.5199829418642494 1105 28-29 0.5520116167625986 1105 30-31 0.5776770421107784 1105 32-33 0.5598201953336712 1105 34-35 0.6345288391331323 1105 36-37 0.6323429149742239 1105 38-39 0.5295332570440934 1105 40-41 0.6100421733280399 1105 42-43 0.5555483250492941 1105 44-45 0.6298946039630948 1105 46-47 0.5652831250726607 1105 48-49 0.4965191974308638 1105 50-51 0.6510841887634484 1105 52-53 0.5220856837247538 1105 54-55 0.6824315966785832 1105 56-57 0.6754215129065173 1105 58-59 0.6303187309974945 1105 60-61 0.663896524198563 1105 62-63 0.5953418122318439 1105 64-65 0.5289148985157865 1105 66-67 0.5240528935223523 1105 68-69 0.5951665438877001 1105 70-71 0.4742020161567737 1105 72-73 0.42320040514930213 1105 74-75 0.6290306174820834 1105 76-77 0.7481879793124051 1105 78-79 0.8013038428204169 1105 80-81 0.6918895367400886 1105 82-83 0.7976853694790584 1105 84-85 0.8084517438381944 1105 86-87 0.8065655458583336 1105 88-89 0.867198205511734 1105 90-91 0.7932277001202195 1105 92-93 0.8284276086562237 1105 94-95 0.5541364544037997 1105 96-97 0.4136596465293927 1105 98-99 0.7134257782832876 1105 100-101 0.957959225501348 1106 1 0.08136086903683193 1106 2 0.12164617181174364 1106 3 0.10143049514731928 1106 4 0.0049507023621586654 1106 5 0.10794504423969897 1106 6 0.1582550082424632 1106 7 0.12666940554662887 1106 8 0.10475680603195059 1106 9 0.1406789778099693 1106 10-11 0.19621881954030584 1106 12-13 0.16774982056195853 1106 14-15 0.18304501019248676 1106 16-17 0.2082740889026553 1106 18-19 0.1391172877197917 1106 20-21 0.1797896755564068 1106 22-23 0.2532394293744815 1106 24-25 0.19576479399770363 1106 26-27 0.2042264918331611 1106 28-29 0.08985024216631388 1106 30-31 0.08055361636244385 1106 32-33 0.17297903008439164 1106 34-35 0.24312996019230582 1106 36-37 0.23319274713173144 1106 38-39 0.177005093664242 1106 40-41 0.26490294517786594 1106 42-43 0.18418224047308485 1106 44-45 0.17336810510740008 1106 46-47 0.1882774609914577 1106 48-49 0.19233562256430048 1106 50-51 0.1761423820517578 1106 52-53 0.1020694297615421 1106 54-55 0.24629121275967236 1106 56-57 0.31111680838389333 1106 58-59 0.3890287453727552 1106 60-61 0.10481113538259024 1106 62-63 0.007322024033229013 1106 64-65 -0.0041421982571705485 1106 66-67 0.03120280263438957 1106 68-69 0.16536063290801195 1106 70-71 0.09553559561477698 1106 72-73 0.1462079818138804 1106 74-75 0.24440094695995995 1106 76-77 0.2315097646311024 1106 78-79 0.2886265190929329 1106 80-81 0.28415705821569404 1106 82-83 0.3472493328587767 1106 84-85 0.2300331812580474 1106 86-87 0.18073048726534324 1106 88-89 0.16783570365948108 1106 90-91 0.1346788259486793 1106 92-93 0.39219497479060195 1106 94-95 0.45091512932388156 1106 96-97 0.41285331366181666 1106 98-99 0.43017205947779047 1106 100-101 0.7181367909695595 1107 1 0.156369702267952 1107 2 0.222824514988627 1107 3 0.21411708669894125 1107 4 0.08035617734753941 1107 5 0.13506875856285205 1107 6 0.18767130640522822 1107 7 0.1433278092253616 1107 8 0.1254073855922897 1107 9 0.12128198602646734 1107 10-11 0.15033950524195916 1107 12-13 0.1395462039169999 1107 14-15 0.17538149183345553 1107 16-17 0.2595280731379077 1107 18-19 0.2178964176168492 1107 20-21 0.28077011035625077 1107 22-23 0.28247512124735863 1107 24-25 0.24259372933908452 1107 26-27 0.22367974404575364 1107 28-29 0.2825407909688096 1107 30-31 0.2844034613792843 1107 32-33 0.30782608902978126 1107 34-35 0.26177187055906614 1107 36-37 0.27472584588696236 1107 38-39 0.41898846376890475 1107 40-41 0.25449698871322113 1107 42-43 0.41971032790220164 1107 44-45 0.41616723997554317 1107 46-47 0.3136444428433336 1107 48-49 0.284725559633749 1107 50-51 0.2602100610333693 1107 52-53 0.1486243504590874 1107 54-55 0.2580990966172436 1107 56-57 0.27849031992887063 1107 58-59 0.2702079795498378 1107 60-61 0.30921228677117796 1107 62-63 0.1725918355303051 1107 64-65 0.11423810913515098 1107 66-67 0.0323971966481551 1107 68-69 0.1917368741494343 1107 70-71 0.2265008682862728 1107 72-73 0.19717812015311864 1107 74-75 0.4457181789175131 1107 76-77 0.4203909667590331 1107 78-79 0.4621483490790048 1107 80-81 0.43681396901076397 1107 82-83 0.4306232554371867 1107 84-85 0.5465827719960821 1107 86-87 0.508408872127653 1107 88-89 0.46314607720718826 1107 90-91 0.43104231222894995 1107 92-93 0.4685999604133677 1107 94-95 0.42689300318566126 1107 96-97 0.35900722468747404 1107 98-99 0.31066542058674074 1107 100-101 0.8046387017387318 1108 1 0.22250000290840433 1108 2 0.3226471402705826 1108 3 0.31866828059504115 1108 4 0.10281963602920996 1108 5 0.2285756102815384 1108 6 0.2006408733122811 1108 7 0.2439833497948456 1108 8 0.28320169410149987 1108 9 0.3049859965483108 1108 10-11 0.3563278723106933 1108 12-13 0.3892125848244987 1108 14-15 0.4872446624026665 1108 16-17 0.5524027795181397 1108 18-19 0.5046890211000274 1108 20-21 0.495026607426432 1108 22-23 0.6208498201874946 1108 24-25 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-1.0102082654690285 2316 86-87 -0.7991722055157133 2316 88-89 -1.0608771867944142 2316 90-91 -0.7994657380497436 2316 92-93 -1.154310778936317 2316 94-95 -0.8190913008145344 2316 96-97 -0.7566326885936405 2316 98-99 -0.8409193148179455 2316 100-101 -1.0655298028946802 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 38.0 4 90.0 5 172.0 6 296.0 7 790.0 8 1814.0 9 3153.0 10 5045.0 11 7948.0 12 10486.0 13 13639.0 14 16130.0 15 19471.0 16 23074.0 17 26002.0 18 24176.0 19 21999.0 20 22818.0 21 24646.0 22 27578.0 23 32561.0 24 40057.0 25 50464.0 26 65124.0 27 83243.0 28 105428.0 29 131526.0 30 160821.0 31 192570.0 32 231383.0 33 279548.0 34 344611.0 35 424498.0 36 546087.0 37 694061.0 38 579428.0 39 96666.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.34226138646048 21.22791496871118 14.521588705838646 23.908234938989704 2 15.387394189116183 21.90282102768513 39.710066396104686 22.999718387094003 3 18.22287104216161 26.490403400547024 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22.38050581334678 27.086308550622135 28.50223629548607 22.03094934054501 24-25 22.2356899042608 27.309328924827813 28.501431595770192 21.953549575141192 26-27 22.140821592783524 27.48710638638704 28.429921773520473 21.94215024730897 28-29 22.066432940181752 27.658050638468435 28.39145328571513 21.884063135634683 30-31 22.543943699315488 27.278514677540343 28.26493054934819 21.912611073795976 32-33 22.3947587614244 27.401048145543104 28.149005343828566 22.055187749203927 34-35 22.476764931787766 27.45020028549855 28.2831520563047 21.789882726408987 36-37 22.460244883669695 27.356328003244624 28.306406623155834 21.877020489929848 38-39 22.422643075834443 27.38848586490896 28.299561502480824 21.88930955677577 40-41 22.423352899018916 27.207535640649482 28.290156571142816 22.078954889188786 42-43 22.358318788457822 27.325520989024447 28.059000632099877 22.257159590417853 44-45 22.311639096710923 27.436706891684064 28.032014086495245 22.21963992510977 46-47 22.42333807007648 27.32150815332374 27.88333518879313 22.371818587806647 48-49 22.43060578821913 27.35841992699629 27.86738878647907 22.343585498305508 50-51 22.38281024661929 27.38307076650533 27.778409693572787 22.455709293302593 52-53 22.432959940761375 27.422683070327984 27.741069784003386 22.403287204907254 54-55 22.23806459897141 27.294032525879086 27.85720637997447 22.610696495175034 56-57 22.339180770592154 27.383322422765893 27.642331825279694 22.63516498136226 58-59 22.33218860909794 27.45118300103667 27.787042049836195 22.42958634002919 60-61 22.515867593153622 27.561697757265573 27.698698711603353 22.22373593797745 62-63 22.439122389170993 27.39218852649306 27.725767182106825 22.442921902229116 64-65 22.153606999426675 27.303443821852802 27.77842848945882 22.7645206892617 66-67 22.267917390325206 27.345902855101656 27.652610899581642 22.733568854991496 68-69 22.161540579084846 27.38458270521655 27.763782614672444 22.690094101026162 70-71 22.18464632231253 27.14917700684894 27.768205327926182 22.897971342912342 72-73 22.230859380036634 27.38642172967449 27.551857117351673 22.8308617729372 74-75 22.193719450025405 27.438907032658555 27.533604244781007 22.83376927253504 76-77 22.096541324566058 27.274431312857182 27.555271944006975 23.07375541856978 78-79 22.00539732139903 27.355007207224567 27.567770373657762 23.071825097718634 80-81 22.135670135211857 27.471096133873935 27.450062688257752 22.943171042656456 82-83 22.20009007628626 27.28695101057236 27.639339657245802 22.873619255895587 84-85 22.218282029117162 27.28828907155134 27.3551033967934 23.138325502538095 86-87 21.94930423033528 27.43065518890483 27.52987839701355 23.090162183746337 88-89 22.018594517292993 27.189551132600997 27.484876501830257 23.30697784827575 90-91 22.1039493162109 27.080969061863268 27.5675872079525 23.24749441397333 92-93 22.134008813549325 27.002730303231186 27.534789394437386 23.328471488782096 94-95 21.921486675147452 26.977049988699637 27.564645683394573 23.53681765275834 96-97 21.825321651274766 26.88492754430685 27.56690717963719 23.72284362478119 98-99 21.61592910903409 26.87897275888807 27.60823083673441 23.896867295343426 100-101 21.698576423228538 26.893842310781764 27.49277996777683 23.91480129821287 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 354.0 1 287.0 2 254.0 3 290.5 4 257.5 5 268.0 6 414.5 7 773.0 8 1217.5 9 1487.0 10 1362.5 11 1163.0 12 1240.5 13 1388.5 14 1655.5 15 1914.5 16 2121.0 17 2445.5 18 2797.5 19 3111.5 20 3507.5 21 4185.5 22 5083.0 23 6348.5 24 8429.5 25 11398.5 26 15803.0 27 21869.5 28 30427.5 29 43041.5 30 57793.0 31 71716.5 32 85263.5 33 97641.0 34 107909.0 35 115464.5 36 124985.0 37 135708.0 38 140446.5 39 141068.5 40 139625.5 41 138188.5 42 139584.0 43 143969.0 44 148500.0 45 153378.0 46 158141.5 47 161296.0 48 163587.0 49 163651.0 50 160956.5 51 156894.0 52 152559.5 53 147323.5 54 140276.5 55 129432.5 56 115277.5 57 101069.0 58 88821.0 59 76465.5 60 62900.5 61 51067.0 62 41157.0 63 32226.5 64 24402.0 65 17807.0 66 12670.5 67 9604.0 68 7208.0 69 4967.5 70 3633.0 71 2558.0 72 1777.0 73 1290.5 74 910.5 75 623.5 76 391.5 77 214.0 78 125.5 79 84.0 80 49.0 81 29.0 82 16.0 83 10.0 84 6.5 85 7.0 86 6.0 87 2.0 88 1.5 89 1.0 90 1.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01880458553300553 2 0.0029948043626638436 3 0.003551977267345489 4 0.035589419286540094 5 0.0019036740909956216 6 2.3215537695068555E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.1607768847534277E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.4823306542602834E-5 24-25 6.732505931569881E-4 26-27 0.0022054760810315127 28-29 8.125438193273994E-5 30-31 0.0069878768462156345 32-33 0.02314589108198335 34-35 0.014115046918601682 36-37 6.26819517766851E-4 38-39 5.571729046816453E-4 40-41 0.0020661828548611014 42-43 0.008996020856839065 44-45 0.008264731419444405 46-47 0.008160261499816596 48-49 0.00971570252538619 50-51 0.1941747572815534 52-53 0.05016877695904314 54-55 0.01850278354296964 56-57 0.010992557098614962 58-59 0.020696651855153618 60-61 0.0552645874831107 62-63 0.09929285472180821 64-65 0.10398239333621205 66-67 0.10843977657366523 68-69 0.10643163256304179 70-71 0.09337289260956573 72-73 0.07105115311575731 74-75 0.24624720833159217 76-77 0.2984009137635637 78-79 0.007510226444354677 80-81 8.009360504798652E-4 82-83 0.0 84-85 1.1607768847534277E-5 86-87 0.0 88-89 1.7411653271301415E-4 90-91 0.006593212705399471 92-93 0.018467960236427036 94-95 0.0017759886336727445 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4307460.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.21479973649457 #Duplication Level Percentage of deduplicated Percentage of total 1 74.29868545405054 39.53789667122102 2 13.854770992073377 14.745577274763576 3 4.883766229326829 7.796659255604473 4 2.2265052243672114 4.739321185078403 5 1.2088173802483857 3.216348740395593 6 0.7715693712030874 2.4635345742831203 7 0.5202337849586592 1.9378895677913541 8 0.35923063169363795 1.5293108899833106 9 0.28056606263771405 1.343724015251047 >10 1.4482277471305205 13.915199995513857 >50 0.10160188363027517 3.6851398441296133 >100 0.04423264391570868 4.116138005599948 >500 0.001311654886710158 0.4320353073816276 >1k 4.3721807040107935E-4 0.40690732981478805 >5k 4.372180704009364E-5 0.13431734318830782 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5773 0.13402329911363078 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4592 0.1066057490957548 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.643107539013711E-5 2 0.0 0.0 0.0 0.0 4.643107539013711E-5 3 0.0 0.0 0.0 0.0 4.643107539013711E-5 4 0.0 0.0 0.0 4.643107539013711E-5 4.643107539013711E-5 5 0.0 0.0 0.0 4.643107539013711E-5 4.643107539013711E-5 6 4.643107539013711E-5 0.0 0.0 4.643107539013711E-5 6.964661308520567E-5 7 4.643107539013711E-5 0.0 0.0 4.643107539013711E-5 6.964661308520567E-5 8 4.643107539013711E-5 0.0 0.0 4.643107539013711E-5 9.286215078027422E-5 9 4.643107539013711E-5 0.0 0.0 4.643107539013711E-5 1.1607768847534278E-4 10-11 4.643107539013711E-5 0.0 0.0 4.643107539013711E-5 1.3929322617041133E-4 12-13 4.643107539013711E-5 0.0 0.0 4.643107539013711E-5 2.08939839255617E-4 14-15 4.643107539013711E-5 0.0 0.0 4.643107539013711E-5 2.08939839255617E-4 16-17 9.286215078027422E-5 0.0 0.0 4.643107539013711E-5 2.3215537695068557E-4 18-19 9.286215078027422E-5 0.0 0.0 4.643107539013711E-5 2.553709146457541E-4 20-21 9.286215078027422E-5 0.0 0.0 8.125438193273994E-5 2.669786834932884E-4 22-23 9.286215078027422E-5 0.0 0.0 1.9733207040808273E-4 2.9019422118835693E-4 24-25 9.286215078027422E-5 0.0 0.0 4.17879678511234E-4 3.134097588834255E-4 26-27 9.286215078027422E-5 0.0 0.0 7.545049750897281E-4 3.366252965784941E-4 28-29 9.286215078027422E-5 0.0 0.0 0.0021822605433364445 3.4823306542602835E-4 30-31 9.286215078027422E-5 0.0 0.0 0.007974537198256049 3.4823306542602835E-4 32-33 9.286215078027422E-5 0.0 0.0 0.025049565172978974 3.714486031210969E-4 34-35 9.286215078027422E-5 0.0 0.0 0.0503545012606037 3.9466414081616545E-4 36-37 1.044699196278085E-4 0.0 0.0 0.08965840657835476 3.9466414081616545E-4 38-39 1.1607768847534278E-4 0.0 0.0 0.1462578874789319 3.9466414081616545E-4 40-41 1.3929322617041133E-4 0.0 0.0 0.20708259624001152 3.9466414081616545E-4 42-43 1.3929322617041133E-4 0.0 0.0 0.2719352007911855 3.9466414081616545E-4 44-45 1.3929322617041133E-4 0.0 0.0 0.3520404136080196 4.17879678511234E-4 46-47 1.3929322617041133E-4 0.0 0.0 0.4461097723484374 4.4109521620630255E-4 48-49 1.3929322617041133E-4 0.0 0.0 0.5449267085474967 4.6431075390137113E-4 50-51 1.6250876386547989E-4 0.0 0.0 0.6465759403453544 4.6431075390137113E-4 52-53 1.8572430156054844E-4 0.0 0.0 0.7503842171488534 4.759185227489054E-4 54-55 1.8572430156054844E-4 0.0 0.0 0.8702344304996448 5.223495981390425E-4 56-57 1.8572430156054844E-4 0.0 0.0 1.0085758196245584 5.571729046816453E-4 58-59 1.8572430156054844E-4 0.0 0.0 1.1612411955073292 5.571729046816453E-4 60-61 1.8572430156054844E-4 0.0 0.0 1.3189211275322348 5.571729046816453E-4 62-63 2.3215537695068557E-4 0.0 0.0 1.4836353674787461 5.687806735291795E-4 64-65 2.3215537695068557E-4 0.0 0.0 1.6672238395713483 5.803884423767139E-4 66-67 2.3215537695068557E-4 0.0 0.0 1.8729715423938935 5.803884423767139E-4 68-69 2.3215537695068557E-4 0.0 0.0 2.1019928217557444 5.803884423767139E-4 70-71 2.3215537695068557E-4 0.0 0.0 2.3478685814842155 6.036039800717825E-4 72-73 2.3215537695068557E-4 0.0 0.0 2.6058396363518175 6.732505931569881E-4 74-75 2.3215537695068557E-4 0.0 0.0 2.8814312843299765 6.732505931569881E-4 76-77 2.4376314579821983E-4 0.0 0.0 3.196849187224025 6.732505931569881E-4 78-79 2.553709146457541E-4 0.0 0.0 3.541356158849995 7.080738996995909E-4 80-81 2.553709146457541E-4 0.0 0.0 3.910808225729316 7.196816685471252E-4 82-83 2.553709146457541E-4 0.0 0.0 4.300446202634499 7.661127439372624E-4 84-85 2.553709146457541E-4 0.0 0.0 4.708772687384213 7.893282816323309E-4 86-87 2.553709146457541E-4 0.0 0.0 5.152978785641654 7.893282816323309E-4 88-89 2.553709146457541E-4 0.0 0.0 5.642420359097937 7.893282816323309E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4945 0.0 52.904224 1 GTATCAA 8620 0.0 41.145123 1 ATCAACG 10970 0.0 31.848238 3 CAACGCA 10930 0.0 31.736217 5 TCAACGC 11100 0.0 31.431747 4 AACGCAG 11645 0.0 29.909521 6 TATCAAC 11785 0.0 29.68741 2 ACGCAGA 13455 0.0 25.603882 7 GTACATG 13330 0.0 25.538422 1 CGCAGAG 13760 0.0 24.794956 8 TACATGG 13990 0.0 23.990324 2 ACATGGG 13840 0.0 23.66613 3 CATGGGG 9805 0.0 21.204628 4 GCAGAGT 16030 0.0 20.72132 9 AGAGTAC 14755 0.0 18.749178 10-11 GAGTACA 10445 0.0 18.546278 1 GTGGTAT 2340 0.0 18.2613 1 CAGAGTA 15645 0.0 18.18304 10-11 GAGTACT 8665 0.0 18.016905 12-13 AGTACAT 10455 0.0 17.481194 2 >>END_MODULE