FastQCFastQC Report
Sat 18 Jun 2016
SRR1930956_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930956_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3746564
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN68090.18173985550493732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29700.052.5688481
GTATCAA59150.047.4267851
ATCAACG71800.038.2093853
TATCAAC71400.038.009272
TCAACGC72200.037.8440484
CAACGCA75450.035.427675
AACGCAG82700.032.406976
ACGCAGA91650.028.9137297
GTACATG76650.027.9911461
CGCAGAG96350.027.0641178
TACATGG78850.026.5638242
ACATGGG79150.025.1214183
GCAGAGT103300.023.4075669
GTACTAG6600.020.6035981
CATGGGG60850.020.0946584
AGAGTAC92400.019.92027910-11
CAGAGTA97650.019.43833710-11
GAGTACT59950.019.166612-13
TAAGGCG10450.018.0395225
GTGGTAT14800.017.3552841
Produced by FastQC (version 0.11.5)