FastQCFastQC Report
Sat 18 Jun 2016
SRR1930956_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930956_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3746564
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59430.15862534311438428No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50380.13446987693257076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44850.055.803111
GTATCAA84550.041.003361
CAACGCA103500.033.0587355
ATCAACG103850.033.008433
TCAACGC104900.032.7228554
TATCAAC106600.032.3367842
AACGCAG111600.030.7443586
ACGCAGA129700.026.0148267
CGCAGAG132950.025.2361098
GTACATG111500.023.2982161
GTGGTAT19150.022.3194711
TACATGG115950.021.9078852
ACATGGG113350.021.5714783
GCAGAGT152000.021.4800939
CATGGGG78550.019.7646884
CAGAGTA147050.018.84686510-11
AGAGTAC140650.018.65849910-11
GAGTACT92350.018.6535312-13
TATACGA2603.2990283E-718.261822
GTACTTT96650.017.60267314-15
Produced by FastQC (version 0.11.5)