##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930956_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3746564 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.251555291728636 31.0 31.0 33.0 30.0 34.0 2 31.37044128967235 31.0 31.0 34.0 30.0 34.0 3 31.44926791588239 31.0 31.0 34.0 30.0 34.0 4 35.32120230696713 37.0 35.0 37.0 33.0 37.0 5 35.10802671461104 37.0 35.0 37.0 32.0 37.0 6 35.14607838008372 37.0 35.0 37.0 32.0 37.0 7 35.025960052997895 36.0 35.0 37.0 32.0 37.0 8 35.003055332832965 36.0 35.0 37.0 32.0 37.0 9 36.50336281456823 38.0 35.0 39.0 32.0 39.0 10-11 36.43037380383733 38.0 35.0 39.0 32.0 39.0 12-13 36.33307585296821 38.0 35.0 39.0 32.0 39.0 14-15 37.35210689047351 39.0 36.0 41.0 32.0 41.0 16-17 37.317878061071426 39.0 36.0 41.0 32.0 41.0 18-19 37.30749948486133 39.0 36.0 40.0 32.0 41.0 20-21 37.269899032820476 39.0 36.0 40.0 31.0 41.0 22-23 37.145563508323896 39.0 36.0 40.0 31.0 41.0 24-25 37.027128990723234 39.0 36.0 40.0 31.0 41.0 26-27 36.75205107933563 39.0 35.5 40.0 30.0 41.0 28-29 36.5687423196294 38.0 35.0 40.0 30.0 41.0 30-31 36.330805239147125 38.0 35.0 40.0 30.0 41.0 32-33 36.09404577100511 38.0 34.5 40.0 30.0 41.0 34-35 36.27991754578328 38.0 35.0 40.0 30.0 41.0 36-37 36.3361604125807 38.0 35.0 40.0 30.0 41.0 38-39 36.297501257151886 38.0 35.0 40.0 30.0 41.0 40-41 36.20218458833213 38.0 35.0 40.0 30.0 41.0 42-43 36.06124011227354 38.0 35.0 40.0 29.5 41.0 44-45 35.876228725840534 38.0 35.0 40.0 29.0 41.0 46-47 35.65505633962212 38.0 34.0 40.0 28.0 41.0 48-49 35.44987727421712 38.0 34.0 40.0 27.0 41.0 50-51 35.17526085234364 38.0 34.0 40.0 26.5 41.0 52-53 34.9574273921385 38.0 33.5 40.0 26.0 41.0 54-55 34.78758657265698 38.0 33.0 40.0 26.0 41.0 56-57 34.52893144224949 37.5 33.0 40.0 26.0 41.0 58-59 34.28198303832525 37.0 33.0 40.0 26.0 41.0 60-61 33.9689071106219 37.0 32.5 40.0 25.0 41.0 62-63 33.66194825445395 36.0 32.0 39.0 24.0 41.0 64-65 33.35339727280783 36.0 32.0 39.0 23.5 40.5 66-67 33.01073944019107 35.5 31.0 39.0 23.0 40.0 68-69 32.638880718439616 35.0 31.0 38.5 22.0 40.0 70-71 32.23012832558045 35.0 31.0 38.0 22.0 40.0 72-73 31.82162122414031 35.0 30.5 37.0 21.0 39.5 74-75 31.2930731998706 34.0 30.0 37.0 20.0 39.0 76-77 29.92253235231001 33.0 28.5 35.0 18.0 38.0 78-79 30.692404827463243 34.0 29.5 36.0 19.5 38.0 80-81 30.699599419628225 34.0 30.0 35.5 19.5 37.5 82-83 30.41887380010057 34.0 30.0 35.0 18.0 37.0 84-85 30.10158294373191 34.0 30.0 35.0 18.0 36.5 86-87 29.770567245081093 34.0 29.0 35.0 16.0 36.0 88-89 29.42502129951604 34.0 29.0 35.0 9.5 36.0 90-91 29.08679072344687 34.0 29.0 35.0 7.0 35.5 92-93 28.72869367772711 33.5 29.0 35.0 2.0 35.0 94-95 28.315331327584424 33.0 29.0 35.0 2.0 35.0 96-97 27.848003664157346 33.0 27.0 35.0 2.0 35.0 98-99 27.359664615364906 33.0 27.0 35.0 2.0 35.0 100-101 26.14788817166876 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5313655299348738 1101 2 0.6452185267462838 1101 3 0.5848386771733836 1101 4 0.4988731603080865 1101 5 0.523093068185247 1101 6 0.4996721869849665 1101 7 0.5458943195211745 1101 8 0.5505406040617373 1101 9 0.717675115893293 1101 10-11 0.6055724708289745 1101 12-13 0.7013190629867339 1101 14-15 0.8909824848273757 1101 16-17 0.8162349392776918 1101 18-19 0.8662902816981344 1101 20-21 0.8108718889290145 1101 22-23 0.8369698029577322 1101 24-25 0.7142361285092633 1101 26-27 0.692787410015427 1101 28-29 0.734929530888742 1101 30-31 0.788787326562236 1101 32-33 0.8092186737673401 1101 34-35 0.8465875187643377 1101 36-37 0.8729751599936577 1101 38-39 0.8923383772869684 1101 40-41 0.8984770202674568 1101 42-43 0.9063615771695623 1101 44-45 0.8989807102146088 1101 46-47 0.9020745483757295 1101 48-49 0.8324407993621961 1101 50-51 0.8564011280812949 1101 52-53 0.9427797939600779 1101 54-55 0.9266776787636459 1101 56-57 1.0512177394423858 1101 58-59 0.8331190222582521 1101 60-61 0.8647285657653185 1101 62-63 0.9143203067133356 1101 64-65 0.9323908262820169 1101 66-67 1.1086388753444325 1101 68-69 0.9773762792252967 1101 70-71 0.7568160438982758 1101 72-73 0.92457130600555 1101 74-75 0.8870136881564541 1101 76-77 0.9374490353739588 1101 78-79 0.9021285168755 1101 80-81 0.8548672555883314 1101 82-83 1.0161954982879244 1101 84-85 0.9257367818147735 1101 86-87 0.9099197577089164 1101 88-89 0.8178711936021301 1101 90-91 0.9811114853402394 1101 92-93 1.1679559524774987 1101 94-95 1.2312404809378386 1101 96-97 1.2661119882917902 1101 98-99 1.1967031771904821 1101 100-101 1.5346462158868377 1102 1 0.41605062216095945 1102 2 0.47525512577701434 1102 3 0.49266705373751307 1102 4 0.3196879830889898 1102 5 0.34406504130664217 1102 6 0.39577798073295156 1102 7 0.40002126785583414 1102 8 0.41801700549240195 1102 9 0.5357145873408484 1102 10-11 0.5375655395623014 1102 12-13 0.5875780522984329 1102 14-15 0.6969224207877502 1102 16-17 0.6556452265540642 1102 18-19 0.6779629441301793 1102 20-21 0.7505473197048076 1102 22-23 0.793961119794325 1102 24-25 0.717102023416281 1102 26-27 0.6855966891706231 1102 28-29 0.7833092865945517 1102 30-31 0.7886756214636961 1102 32-33 0.7685160417593195 1102 34-35 0.8790534955421663 1102 36-37 0.8953640880063105 1102 38-39 0.8758669054508701 1102 40-41 0.8357513117900126 1102 42-43 0.7486531621677699 1102 44-45 0.9109936915959267 1102 46-47 1.0074613012611522 1102 48-49 0.7933131051705189 1102 50-51 0.8020380726346232 1102 52-53 0.7891076102641605 1102 54-55 0.7640292271975255 1102 56-57 0.6723693079970516 1102 58-59 0.8768951749542637 1102 60-61 0.7161712960877011 1102 62-63 0.5862199872090059 1102 64-65 0.6716453508588742 1102 66-67 0.7252865082023874 1102 68-69 0.8334505735231303 1102 70-71 0.7536161091173952 1102 72-73 0.7389185770361202 1102 74-75 0.872644457707441 1102 76-77 0.9388999180870741 1102 78-79 1.0591785309253652 1102 80-81 0.8946538710973009 1102 82-83 1.0241834882552787 1102 84-85 1.1666887035240698 1102 86-87 1.0660661052644151 1102 88-89 1.0668739654149775 1102 90-91 1.059667506140979 1102 92-93 1.1993357532816376 1102 94-95 1.082674607886176 1102 96-97 1.0487718878959669 1102 98-99 1.0620767821776624 1102 100-101 1.2742483792191202 1103 1 0.4107310418405099 1103 2 0.4757107223099304 1103 3 0.4432020648755959 1103 4 0.2802653437187601 1103 5 0.2889256754780618 1103 6 0.3933181656333886 1103 7 0.4062351848473611 1103 8 0.44425354390108396 1103 9 0.43722179273878936 1103 10-11 0.5423739784827859 1103 12-13 0.5176363686546424 1103 14-15 0.653663157657256 1103 16-17 0.6966387262137559 1103 18-19 0.643784166696733 1103 20-21 0.6346860750475756 1103 22-23 0.6602642524706681 1103 24-25 0.6067180916087196 1103 26-27 0.7190384562840322 1103 28-29 0.6641228642106753 1103 30-31 0.7584772887168398 1103 32-33 0.8161049612725364 1103 34-35 0.649395643799636 1103 36-37 0.6475861534738385 1103 38-39 0.70799004215921 1103 40-41 0.6729109226225702 1103 42-43 0.6328230239900208 1103 44-45 0.7555740425095152 1103 46-47 0.654212667813816 1103 48-49 0.6299840836442954 1103 50-51 0.4351759710768093 1103 52-53 0.40014383039161316 1103 54-55 0.5247486418785101 1103 56-57 0.549061835577568 1103 58-59 0.6737659714331343 1103 60-61 0.520342265422471 1103 62-63 0.46529447906993937 1103 64-65 0.42913317803709816 1103 66-67 0.41208789018304515 1103 68-69 0.3872575395061162 1103 70-71 0.23404451472855925 1103 72-73 0.30702528302444776 1103 74-75 0.4534631531687161 1103 76-77 0.6315272491944626 1103 78-79 0.6904729219701657 1103 80-81 0.6311696353466054 1103 82-83 0.6072371453415109 1103 84-85 0.6955482052707005 1103 86-87 0.6570521515543781 1103 88-89 0.5869486114882321 1103 90-91 0.7619251528176711 1103 92-93 0.7541807414692983 1103 94-95 0.6654155077685324 1103 96-97 0.6043604191028393 1103 98-99 0.8594618002410854 1103 100-101 1.1317676378193688 1104 1 0.40183621979446116 1104 2 0.5125459771620946 1104 3 0.4833397188224069 1104 4 0.3167021266196315 1104 5 0.39718801915152824 1104 6 0.36313384921968606 1104 7 0.4195079100768879 1104 8 0.44155566139478 1104 9 0.5751192648745089 1104 10-11 0.5289453811794047 1104 12-13 0.5516014091529584 1104 14-15 0.6401772816634761 1104 16-17 0.5966414364576167 1104 18-19 0.7383918721183491 1104 20-21 0.7533652410063851 1104 22-23 0.6546319360516861 1104 24-25 0.6569273092142041 1104 26-27 0.7072356747805841 1104 28-29 0.7419263635982176 1104 30-31 0.7725553860463208 1104 32-33 0.840293689434418 1104 34-35 0.6948563488993216 1104 36-37 0.6176871398042962 1104 38-39 0.6394573285242373 1104 40-41 0.6262221465356461 1104 42-43 0.6019979993168789 1104 44-45 0.708071003400697 1104 46-47 0.7475839401543212 1104 48-49 0.7323926971959125 1104 50-51 0.7600450365516735 1104 52-53 0.6663285143408331 1104 54-55 0.6588617542366251 1104 56-57 0.7284118926579524 1104 58-59 0.6655495366465587 1104 60-61 0.5727885579741283 1104 62-63 0.49913712892163176 1104 64-65 0.5957879570894278 1104 66-67 0.6195274104302229 1104 68-69 0.6000068241008947 1104 70-71 0.589193713653188 1104 72-73 0.6081525461606994 1104 74-75 0.7999362335062905 1104 76-77 0.9181653172797546 1104 78-79 0.9281844779872692 1104 80-81 0.6927172242541744 1104 82-83 0.7076411038840327 1104 84-85 0.8768510922092432 1104 86-87 0.9293869076555659 1104 88-89 0.8981037438638104 1104 90-91 0.9201118410252711 1104 92-93 0.9453638554601724 1104 94-95 0.9104362940168862 1104 96-97 0.9918295405012714 1104 98-99 1.0094344994919346 1104 100-101 1.3601957492619512 1105 1 0.17754019044728153 1105 2 0.2922748515840645 1105 3 0.29142440197102815 1105 4 0.1099498209542844 1105 5 0.06750306216478208 1105 6 0.17718807433529093 1105 7 0.22114383453889985 1105 8 0.23265010971429234 1105 9 0.2804177742650893 1105 10-11 0.32048417060822487 1105 12-13 0.2854646580098219 1105 14-15 0.4015418867940568 1105 16-17 0.34550830204442917 1105 18-19 0.3777141227577303 1105 20-21 0.4315463487590989 1105 22-23 0.5439299675172364 1105 24-25 0.42477328818549864 1105 26-27 0.4858310918670483 1105 28-29 0.5039926789254068 1105 30-31 0.503258194178791 1105 32-33 0.621196063559303 1105 34-35 0.5453113464451178 1105 36-37 0.4960810533644491 1105 38-39 0.4133138605968796 1105 40-41 0.4798779500955419 1105 42-43 0.3927740462310396 1105 44-45 0.3233736461969485 1105 46-47 0.3071563264210866 1105 48-49 0.33980182685567684 1105 50-51 0.3919058441158043 1105 52-53 0.2826067054591732 1105 54-55 0.33437100273180675 1105 56-57 0.38151775951062206 1105 58-59 0.307798432252234 1105 60-61 0.3983381717590291 1105 62-63 0.23858564218728162 1105 64-65 0.24478011653554432 1105 66-67 0.2738768511287688 1105 68-69 0.3167464712113883 1105 70-71 0.35938410865176706 1105 72-73 0.3473097834956853 1105 74-75 0.35649091071314487 1105 76-77 0.4853748163626541 1105 78-79 0.5022408989746374 1105 80-81 0.5550286376205769 1105 82-83 0.5916251256877487 1105 84-85 0.6369246539132405 1105 86-87 0.7130137773757887 1105 88-89 0.641433137544869 1105 90-91 0.4378238072346825 1105 92-93 0.4532683479554578 1105 94-95 0.5286474981296259 1105 96-97 0.31942809639266656 1105 98-99 0.616029219066192 1105 100-101 0.9099057650205573 1106 1 0.10831460962659278 1106 2 0.16854062968458905 1106 3 0.22640249039394789 1106 4 0.07857453539777026 1106 5 0.10009848720903136 1106 6 0.04145064512557184 1106 7 0.08193383640479368 1106 8 0.16576167973300215 1106 9 0.19474123856359427 1106 10-11 0.11002069914874824 1106 12-13 0.11492368916005802 1106 14-15 0.20980468199520175 1106 16-17 0.17380682647999635 1106 18-19 0.20695775730590782 1106 20-21 0.20324706788243674 1106 22-23 0.2734264425017301 1106 24-25 0.21834681678149792 1106 26-27 0.2020675956971658 1106 28-29 0.20592601629309115 1106 30-31 0.12042348160120042 1106 32-33 0.2044716552223136 1106 34-35 0.19346329781099314 1106 36-37 0.3116723129397272 1106 38-39 0.34469097421938244 1106 40-41 0.25009528822975113 1106 42-43 0.19885482405420163 1106 44-45 0.14041475120609448 1106 46-47 0.1640344561961129 1106 48-49 0.3249521317014441 1106 50-51 0.2509347250368492 1106 52-53 0.19112290842744528 1106 54-55 0.28835549316787024 1106 56-57 0.3523596028062812 1106 58-59 0.3779426477471688 1106 60-61 0.14130049594951544 1106 62-63 0.04006946443371362 1106 64-65 0.15387512301572315 1106 66-67 0.16029867042003332 1106 68-69 0.2247933655163905 1106 70-71 0.20616116528903206 1106 72-73 0.18832736066528355 1106 74-75 0.422416643326347 1106 76-77 0.38772778942830755 1106 78-79 0.4691095354139172 1106 80-81 0.37781611045173236 1106 82-83 0.4597747320862311 1106 84-85 0.41094777327460363 1106 86-87 0.4035486190526676 1106 88-89 0.5216439450709522 1106 90-91 0.40856107254883156 1106 92-93 0.5463921723199192 1106 94-95 0.48134578655557547 1106 96-97 0.3888183922619959 1106 98-99 0.3845271035525961 1106 100-101 0.7002294965949112 1107 1 0.11718565721673002 1107 2 0.1439856493617917 1107 3 0.12279703170127831 1107 4 0.07510302002326341 1107 5 0.13582576810788538 1107 6 0.18297582339916119 1107 7 0.11972267056525965 1107 8 0.07649776133546737 1107 9 0.1099699021130931 1107 10-11 0.13290493293637695 1107 12-13 0.05588487025825373 1107 14-15 0.06986131845320642 1107 16-17 0.1465143803628095 1107 18-19 0.2054308429355558 1107 20-21 0.16812942451542767 1107 22-23 0.21094810517935514 1107 24-25 0.21100308429428338 1107 26-27 0.15826586189381686 1107 28-29 0.3227353663578967 1107 30-31 0.17372384101515337 1107 32-33 0.3195868621353313 1107 34-35 0.12827175442713212 1107 36-37 0.18907832666550206 1107 38-39 0.29212509594368186 1107 40-41 0.23944911868436947 1107 42-43 0.32760070251978846 1107 44-45 0.31996128100389143 1107 46-47 0.27099971835474435 1107 48-49 0.27319428440225124 1107 50-51 0.20202805910341937 1107 52-53 0.09174055444545104 1107 54-55 0.3218096956626013 1107 56-57 0.23408535944176379 1107 58-59 0.2470979787171288 1107 60-61 0.1754856654661694 1107 62-63 0.18810744081071107 1107 64-65 0.1700395957435319 1107 66-67 0.2797452723126952 1107 68-69 0.32243543045060363 1107 70-71 0.2793794435767154 1107 72-73 0.2348416531929054 1107 74-75 0.4026873153622539 1107 76-77 0.42148365106682206 1107 78-79 0.5352337067760402 1107 80-81 0.49856654197905215 1107 82-83 0.49149225212625325 1107 84-85 0.37491180499930365 1107 86-87 0.510103500240394 1107 88-89 0.5917675198964147 1107 90-91 0.4862460701203908 1107 92-93 0.4117997598765939 1107 94-95 0.4162731682664109 1107 96-97 0.5216707314366111 1107 98-99 0.4655150005660502 1107 100-101 0.837345075387752 1108 1 0.24404327954194116 1108 2 0.24391176765901434 1108 3 0.3428739197276194 1108 4 0.12848248832036546 1108 5 0.1908860389471556 1108 6 0.18462787626832267 1108 7 0.22690554091526138 1108 8 0.339617804727645 1108 9 0.3052780174394343 1108 10-11 0.3276885526119102 1108 12-13 0.33836248981507566 1108 14-15 0.3155475353518895 1108 16-17 0.44338489790772684 1108 18-19 0.3813393109162888 1108 20-21 0.3619672790859525 1108 22-23 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-0.7796411264758696 2316 78-79 -0.6734747831926562 2316 80-81 -0.5640928770654554 2316 82-83 -0.8373681480635895 2316 84-85 -0.811728350788151 2316 86-87 -0.8193926602725874 2316 88-89 -0.9201929156134447 2316 90-91 -0.5913707884921742 2316 92-93 -0.9701978811559329 2316 94-95 -0.7611380299623534 2316 96-97 -0.6208287345899706 2316 98-99 -0.5659134798119929 2316 100-101 -0.7782395126408481 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 19.0 4 53.0 5 107.0 6 251.0 7 652.0 8 1600.0 9 2889.0 10 4708.0 11 7385.0 12 10057.0 13 12824.0 14 15029.0 15 18445.0 16 21854.0 17 24835.0 18 22667.0 19 20211.0 20 20617.0 21 22040.0 22 25145.0 23 29123.0 24 35333.0 25 44684.0 26 57049.0 27 72928.0 28 92355.0 29 114527.0 30 139798.0 31 168560.0 32 201509.0 33 243155.0 34 297650.0 35 368516.0 36 476957.0 37 599512.0 38 489996.0 39 83511.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.257726935988735 21.380443560497355 14.752798115234961 23.60903138827895 2 15.453924499262897 21.94872607954192 39.53145223435556 23.06589718683962 3 18.308229805582343 26.508567247817183 30.942080957842677 24.241121988757797 4 13.07319099784983 18.12295626258416 38.38673873242689 30.417114007139112 5 12.944094962332803 38.24563005005211 35.62873148670118 13.18154350091392 6 30.37203174537082 38.545685350996465 17.743013461408086 13.339269442224634 7 26.089504943729775 33.07163577080226 23.29617217268943 17.542687112778534 8 24.97627159178383 36.34794440986461 20.946285716726045 17.72949828162551 9 25.62772182725292 16.019024364724586 20.986802841216644 37.36645096680585 10-11 24.253916388456194 26.764870425275 29.137084005504775 19.84412918076403 12-13 24.766439329476288 24.29720938972349 29.160985906019487 21.775365374780733 14-15 22.023662484300612 25.87600610532826 26.54190967269072 25.55842173768041 16-17 21.7844937387964 28.837876518324524 27.309289258104226 22.06834048477485 18-19 21.83257779661578 27.810174869560484 29.071143586496856 21.286103747326884 20-21 22.470495632798478 27.031034302363445 29.021724972534834 21.47674509230324 22-23 22.244534524042436 26.91413436795271 28.86298722322299 21.978343884781864 24-25 22.17704729181297 27.107814208656666 28.814038633440337 21.90109986609003 26-27 22.098912351959314 27.277401965605836 28.674860939412838 21.948824743022012 28-29 22.13668748486946 27.422935326556807 28.6234095748074 21.81696761376633 30-31 22.38264859355287 27.14471859779572 28.544152145789653 21.92848066286176 32-33 22.255957237319844 27.278575196705766 28.372831110693447 22.092636455280942 34-35 22.38025602543917 27.25749419252468 28.510005515166174 21.852244266869974 36-37 22.445036902750047 27.099655908864094 28.44653989090991 22.00876729747595 38-39 22.272071212419466 27.152003683401006 28.47179562462674 22.104129479552785 40-41 22.365452830634382 27.15007286165814 28.54679594878771 21.937678358919772 42-43 22.37332564558408 27.184201675288687 28.205764744195484 22.236707934931747 44-45 22.284814028394674 27.27942127426489 28.28296336811096 22.152801329229472 46-47 22.5157914644477 27.27371128743862 27.980071263291794 22.230425984821885 48-49 22.446729678477467 27.35797784193802 27.823078787542283 22.37221369204223 50-51 22.477303868153903 27.361319691161718 27.8176559211187 22.343720519565682 52-53 22.53987515114744 27.384938344663162 27.761031092577003 22.314155411612397 54-55 22.361676939564873 27.319484353323986 27.868637358128357 22.45020134898279 56-57 22.365050740902735 27.50980042254166 27.681497178628828 22.44365165792678 58-59 22.372247466947446 27.427887458329742 27.797984236648364 22.40188083807445 60-61 22.363889192293527 27.510140762245626 27.862520399696518 22.26344964576433 62-63 22.368944671056717 27.43939411143535 27.82085971360691 22.37080150390102 64-65 22.17795899871779 27.37258670804788 27.748312008167233 22.701142285067093 66-67 22.214878793344155 27.453023653500598 27.701288551331636 22.63080900182361 68-69 22.327360200585254 27.441626890907187 27.726104618975654 22.5049082895319 70-71 22.230418060790385 27.244494658426778 27.764292194795104 22.760795085987734 72-73 22.254018909561783 27.384742647157022 27.654673195997997 22.7065652472832 74-75 22.206341350965783 27.602332280539937 27.49481864834386 22.696507720150414 76-77 22.218469730172618 27.443237087935877 27.52321737329751 22.815075808593992 78-79 22.152250644171836 27.506250862521597 27.534025058971785 22.80747343433478 80-81 22.305576300655485 27.54798168120188 27.396041247723897 22.75040077041874 82-83 22.30633188169213 27.463403801456483 27.52556742658073 22.70469689027066 84-85 22.30849386264321 27.503333721244317 27.253037716691882 22.93513469942059 86-87 22.195977434257095 27.62640648871873 27.32182074028363 22.855795336740545 88-89 22.183192339531775 27.488433054379623 27.253404089805596 23.074970516283006 90-91 22.118677592238566 27.498266297735537 27.371128131674887 23.01192797835101 92-93 22.18654723867485 27.380941416453464 27.39435624251923 23.038155102352455 94-95 22.051994030706467 27.343162577835834 27.34543135779541 23.25941203366229 96-97 22.022471790152256 27.16854696730124 27.424341343161363 23.384639899385142 98-99 21.844081670565348 27.329294254682424 27.338702875488046 23.48792119926418 100-101 21.875790724514516 27.271214371354656 27.27930178157907 23.573693122551756 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 258.0 1 211.5 2 198.0 3 258.0 4 240.5 5 254.0 6 413.5 7 773.5 8 1214.0 9 1472.5 10 1392.5 11 1259.5 12 1323.0 13 1479.5 14 1708.5 15 1981.0 16 2236.5 17 2504.0 18 2875.5 19 3280.0 20 3764.5 21 4463.0 22 5593.0 23 7160.0 24 9393.5 25 12965.0 26 17803.0 27 24390.0 28 32787.5 29 43684.0 30 55423.0 31 66605.0 32 79427.5 33 87597.5 34 91773.0 35 96328.5 36 103018.5 37 111129.0 38 114404.0 39 114307.5 40 115132.5 41 116764.5 42 118277.5 43 120201.5 44 123965.0 45 130043.0 46 134150.0 47 136086.5 48 138189.0 49 138755.5 50 135433.5 51 131198.0 52 130282.5 53 129883.0 54 125963.0 55 116387.0 56 104862.0 57 93130.0 58 80846.5 59 69660.0 60 58680.5 61 48243.5 62 38876.0 63 29435.5 64 21172.0 65 15314.0 66 10779.0 67 8055.0 68 5965.0 69 4251.0 70 3092.5 71 2110.0 72 1418.5 73 968.5 74 641.5 75 434.5 76 280.5 77 167.5 78 96.5 79 47.0 80 32.0 81 23.5 82 13.0 83 9.0 84 6.0 85 6.5 86 6.5 87 2.5 88 1.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01972473978824331 2 0.0036566838308380693 3 0.0038702128136607304 4 0.03419132837447859 5 0.0018149963539926183 6 5.3382245705665244E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.3345561426416311E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.6691122852832622E-5 24-25 4.537490884981546E-4 26-27 0.0022820910039171895 28-29 8.007336855849787E-5 30-31 0.0070064197488685625 32-33 0.023167894636258717 34-35 0.01414629511200129 36-37 7.340058784528972E-4 38-39 7.073147556000645E-4 40-41 0.0021486353896530263 42-43 0.009181746261374421 44-45 0.00890148947141968 46-47 0.008421049260068692 48-49 0.010356155666899057 50-51 0.19552582045842537 52-53 0.050072546471913996 54-55 0.018803896049820582 56-57 0.011330381651027448 58-59 0.021005913685179275 60-61 0.05399614153128039 62-63 0.09692881264006166 64-65 0.10150634020932246 66-67 0.10647088905994934 68-69 0.10452243709169254 70-71 0.09267157854503487 72-73 0.07041118208577246 74-75 0.24992232883249824 76-77 0.3007689178671444 78-79 0.007500205521645967 80-81 8.007336855849786E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.201100528377468E-4 90-91 0.006566016221796826 92-93 0.018910660541231913 94-95 0.0015213940026114594 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3746564.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.654241330056664 #Duplication Level Percentage of deduplicated Percentage of total 1 76.79942465001966 42.74213713481698 2 12.842761596569288 14.295083064797018 3 4.13993357049473 6.912145860681505 4 1.915037405204327 4.263198156213085 5 1.0262129364672823 2.855655121108812 6 0.6947886499145559 2.3200761117437367 7 0.47427770098634026 1.8476895939710765 8 0.343371233626691 1.5288052401647303 9 0.2604137317338157 1.3043815804416972 >10 1.3812255071164599 13.962934227466375 >50 0.0864172642190171 3.264177097577518 >100 0.03373782862373453 3.2511425103057547 >500 0.0015346720316176095 0.5788731003653556 >1k 7.673359933028722E-4 0.580607998140561 >5k 9.591699916285878E-5 0.2930932022057411 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5943 0.15862534311438428 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5038 0.13446987693257076 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.3382245705665244E-5 2 0.0 0.0 0.0 0.0 5.3382245705665244E-5 3 0.0 0.0 0.0 0.0 5.3382245705665244E-5 4 0.0 0.0 0.0 0.0 5.3382245705665244E-5 5 2.6691122852832622E-5 0.0 0.0 0.0 8.007336855849787E-5 6 2.6691122852832622E-5 0.0 0.0 0.0 1.0676449141133049E-4 7 2.6691122852832622E-5 0.0 0.0 0.0 1.0676449141133049E-4 8 2.6691122852832622E-5 0.0 0.0 0.0 1.0676449141133049E-4 9 2.6691122852832622E-5 0.0 0.0 0.0 1.0676449141133049E-4 10-11 2.6691122852832622E-5 0.0 0.0 0.0 1.201100528377468E-4 12-13 2.6691122852832622E-5 0.0 0.0 0.0 3.336390356604078E-4 14-15 2.6691122852832622E-5 1.3345561426416311E-5 0.0 0.0 4.1371240421890563E-4 16-17 4.003668427924893E-5 2.6691122852832622E-5 0.0 0.0 5.071313342038199E-4 18-19 5.3382245705665244E-5 2.6691122852832622E-5 0.0 0.0 5.071313342038199E-4 20-21 5.3382245705665244E-5 2.6691122852832622E-5 0.0 4.003668427924893E-5 5.872047027623177E-4 22-23 5.3382245705665244E-5 2.6691122852832622E-5 0.0 1.7349229854341205E-4 6.138958256151503E-4 24-25 5.3382245705665244E-5 2.6691122852832622E-5 0.0 3.7367571993965673E-4 6.138958256151503E-4 26-27 5.3382245705665244E-5 2.6691122852832622E-5 0.0 6.672780713208155E-4 6.672780713208156E-4 28-29 5.3382245705665244E-5 2.6691122852832622E-5 0.0 0.0017349229854341204 6.672780713208156E-4 30-31 8.007336855849787E-5 2.6691122852832622E-5 0.0 0.005578444676242018 6.672780713208156E-4 32-33 8.007336855849787E-5 2.6691122852832622E-5 0.0 0.017896397872824273 6.939691941736482E-4 34-35 8.007336855849787E-5 2.6691122852832622E-5 0.0 0.0373942631168185 7.74042562732146E-4 36-37 8.007336855849787E-5 2.6691122852832622E-5 0.0 0.0678221431690477 7.74042562732146E-4 38-39 8.007336855849787E-5 2.6691122852832622E-5 0.0 0.11276999405321783 8.007336855849786E-4 40-41 8.007336855849787E-5 2.6691122852832622E-5 0.0 0.1615079843824902 8.007336855849786E-4 42-43 9.341892998491417E-5 2.6691122852832622E-5 0.0 0.21577103714229892 8.14079247011395E-4 44-45 1.0676449141133049E-4 2.6691122852832622E-5 0.0 0.2813778171145615 8.808070541434765E-4 46-47 1.0676449141133049E-4 2.6691122852832622E-5 0.0 0.35887549231776106 9.341892998491418E-4 48-49 1.0676449141133049E-4 2.6691122852832622E-5 0.0 0.4384550751034815 9.742259841283908E-4 50-51 1.0676449141133049E-4 2.6691122852832622E-5 0.0 0.5226255310198892 9.87571545554807E-4 52-53 1.6014673711699573E-4 2.6691122852832622E-5 0.0 0.6106395086271048 0.0010409537912604724 54-55 1.6014673711699573E-4 2.6691122852832622E-5 0.0 0.7061270006331135 0.0010542993526868887 56-57 1.6014673711699573E-4 2.6691122852832622E-5 0.0 0.8164013746995914 0.0010809904755397213 58-59 1.6014673711699573E-4 2.6691122852832622E-5 0.0 0.9379394026099648 0.0010943360369661374 60-61 1.6014673711699573E-4 2.6691122852832622E-5 0.0 1.0699403506786485 0.0010943360369661374 62-63 1.7349229854341205E-4 2.6691122852832622E-5 0.0 1.20649213519374 0.00112102715981897 64-65 1.8683785996982837E-4 2.6691122852832622E-5 0.0 1.3528395617958213 0.00112102715981897 66-67 1.8683785996982837E-4 2.6691122852832622E-5 0.0 1.5211671280672103 0.001161063844098219 68-69 2.1352898282266098E-4 2.6691122852832622E-5 0.0 1.7118218186049938 0.001201100528377468 70-71 2.1352898282266098E-4 2.6691122852832622E-5 0.0 1.914073802022333 0.0012144460898038843 72-73 2.1352898282266098E-4 2.6691122852832622E-5 0.0 2.1263082653866316 0.001241137212656717 74-75 2.1352898282266098E-4 2.6691122852832622E-5 0.0 2.354263800111249 0.0012811738969359659 76-77 2.1352898282266098E-4 2.6691122852832622E-5 0.0 2.6057742507534902 0.0012811738969359659 78-79 2.1352898282266098E-4 2.6691122852832622E-5 0.0 2.8959467928480604 0.0012811738969359659 80-81 2.1352898282266098E-4 2.6691122852832622E-5 0.0 3.2067782640307225 0.0012945194583623822 82-83 2.1352898282266098E-4 2.6691122852832622E-5 0.0 3.533731173416496 0.0013479017040680474 84-85 2.1352898282266098E-4 2.6691122852832622E-5 0.0 3.874016298667259 0.0013612472654944638 86-87 2.5356566710190993E-4 2.6691122852832622E-5 0.0 4.249934072926553 0.00137459282692088 88-89 2.6691122852832625E-4 2.6691122852832622E-5 0.0 4.656146271623813 0.0014012839497737127 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4485 0.0 55.80311 1 GTATCAA 8455 0.0 41.00336 1 CAACGCA 10350 0.0 33.058735 5 ATCAACG 10385 0.0 33.00843 3 TCAACGC 10490 0.0 32.722855 4 TATCAAC 10660 0.0 32.336784 2 AACGCAG 11160 0.0 30.744358 6 ACGCAGA 12970 0.0 26.014826 7 CGCAGAG 13295 0.0 25.236109 8 GTACATG 11150 0.0 23.298216 1 GTGGTAT 1915 0.0 22.319471 1 TACATGG 11595 0.0 21.907885 2 ACATGGG 11335 0.0 21.571478 3 GCAGAGT 15200 0.0 21.480093 9 CATGGGG 7855 0.0 19.764688 4 CAGAGTA 14705 0.0 18.846865 10-11 AGAGTAC 14065 0.0 18.658499 10-11 GAGTACT 9235 0.0 18.65353 12-13 TATACGA 260 3.2990283E-7 18.26182 2 GTACTTT 9665 0.0 17.602673 14-15 >>END_MODULE