FastQCFastQC Report
Sat 18 Jun 2016
SRR1930955_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930955_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3596268
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN65180.1812434445931171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29600.049.1009371
GTATCAA55850.044.0946271
ATCAACG66250.036.3903663
TCAACGC68000.035.6643144
CAACGCA69200.033.978255
TATCAAC70500.033.9180952
AACGCAG75400.031.3230116
ACGCAGA85050.027.148027
GTACATG72050.027.0359951
CGCAGAG86450.026.2859958
TACATGG75300.025.391392
ACATGGG77700.024.7962933
GCAGAGT94450.022.5468229
CATGGGG58050.022.0612034
AGAGTAC86900.018.86905110-11
CAGAGTA91550.018.32100710-11
TGGTATC15500.018.2263012
GAGTACT58500.017.9905212-13
GAGTACA55300.017.3387371
GTGGTAT17000.016.9205681
Produced by FastQC (version 0.11.5)