FastQCFastQC Report
Sat 18 Jun 2016
SRR1930955_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930955_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3596268
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59840.16639471807996511No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50380.14008967073644124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42900.050.365111
GTATCAA77250.041.5550541
TCAACGC95100.033.3432924
ATCAACG95700.033.1351663
CAACGCA95600.033.05815
TATCAAC98550.032.6599732
AACGCAG101350.031.4166856
ACGCAGA118800.026.602317
CGCAGAG121850.025.8974918
GTACATG98550.023.6592251
GCAGAGT138750.022.0594339
TACATGG102600.022.0243112
ACATGGG100350.021.5237853
AGAGTAC127400.019.64810410-11
GTGGTAT21350.019.573141
CATGGGG65150.019.4540214
GAGTACT86800.019.40779712-13
CAGAGTA134100.018.8256710-11
ATAACGC3208.35098E-917.8014723
GTACTTT94250.017.7226614-15
Produced by FastQC (version 0.11.5)