##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930955_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3596268 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.24948780235511 31.0 31.0 33.0 30.0 34.0 2 31.36225581630735 31.0 31.0 34.0 30.0 34.0 3 31.446331029834262 31.0 31.0 34.0 30.0 34.0 4 35.31444736599163 37.0 35.0 37.0 33.0 37.0 5 35.1015416537366 37.0 35.0 37.0 32.0 37.0 6 35.14005908347209 37.0 35.0 37.0 32.0 37.0 7 35.02460356124738 36.0 35.0 37.0 32.0 37.0 8 35.00097907052533 36.0 35.0 37.0 32.0 37.0 9 36.50663298730795 38.0 35.0 39.0 32.0 39.0 10-11 36.43719377977392 38.0 35.0 39.0 32.0 39.0 12-13 36.33888255825205 38.0 35.0 39.0 32.0 39.0 14-15 37.35493850847601 39.0 36.0 41.0 32.0 41.0 16-17 37.31913583748486 39.0 36.0 41.0 32.0 41.0 18-19 37.31212009227343 39.0 36.0 40.0 32.0 41.0 20-21 37.278073964454265 39.0 36.0 40.0 31.0 41.0 22-23 37.15158561597745 39.0 36.0 40.0 31.0 41.0 24-25 37.03490368348521 39.0 36.0 40.0 31.0 41.0 26-27 36.76435488122687 39.0 35.5 40.0 30.5 41.0 28-29 36.578734538137866 38.0 35.0 40.0 30.0 41.0 30-31 36.3400085866793 38.0 35.0 40.0 30.0 41.0 32-33 36.10944137088782 38.0 35.0 40.0 30.0 41.0 34-35 36.30513410012824 38.0 35.0 40.0 30.0 41.0 36-37 36.35833703161166 38.0 35.0 40.0 30.0 41.0 38-39 36.32288277736809 38.0 35.0 40.0 30.0 41.0 40-41 36.22881915363371 38.0 35.0 40.0 30.0 41.0 42-43 36.090228814982645 38.0 35.0 40.0 29.5 41.0 44-45 35.90756765068677 38.0 35.0 40.0 29.0 41.0 46-47 35.68603132469549 38.0 34.0 40.0 28.0 41.0 48-49 35.48826825475743 38.0 34.0 40.0 27.5 41.0 50-51 35.220951831176095 38.0 34.0 40.0 27.0 41.0 52-53 35.011990485692394 38.0 33.5 40.0 26.0 41.0 54-55 34.850397551016776 38.0 33.0 40.0 26.0 41.0 56-57 34.60859855272188 38.0 33.0 40.0 26.0 41.0 58-59 34.37389732912008 37.0 33.0 40.0 26.0 41.0 60-61 34.0688114734497 37.0 32.5 40.0 25.0 41.0 62-63 33.77035512926178 36.5 32.0 39.5 24.5 41.0 64-65 33.468180764058744 36.0 32.0 39.0 23.5 41.0 66-67 33.14269584469233 36.0 31.5 39.0 23.0 40.0 68-69 32.78075688463707 35.0 31.0 39.0 22.5 40.0 70-71 32.38120546077211 35.0 31.0 38.0 22.0 40.0 72-73 31.963749781718157 35.0 30.5 37.5 21.5 40.0 74-75 31.439258281084726 34.0 30.0 37.0 20.0 39.0 76-77 30.068412309649894 33.5 28.5 35.5 18.5 38.5 78-79 30.84061463161255 34.0 30.0 36.0 20.0 39.0 80-81 30.83421966883447 34.0 30.0 36.0 20.0 38.0 82-83 30.549553453747052 34.0 30.0 35.0 18.5 37.0 84-85 30.215831801189456 34.0 30.0 35.0 18.0 37.0 86-87 29.86143621665571 34.0 29.0 35.0 16.0 36.0 88-89 29.503394630211098 34.0 29.0 35.0 11.0 36.0 90-91 29.15571726022643 34.0 29.0 35.0 7.0 36.0 92-93 28.79027508517163 34.0 29.0 35.0 2.0 35.0 94-95 28.36035106393628 33.0 29.0 35.0 2.0 35.0 96-97 27.887859164000012 33.0 27.5 35.0 2.0 35.0 98-99 27.387715264824536 33.0 27.0 35.0 2.0 35.0 100-101 26.154786851258024 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5147781814292607 1101 2 0.6381520828507838 1101 3 0.5777020642577462 1101 4 0.41093698970369275 1101 5 0.46288272311397094 1101 6 0.46789456912492255 1101 7 0.5548593478331014 1101 8 0.4574243913578897 1101 9 0.7310630815412509 1101 10-11 0.6886447317227251 1101 12-13 0.6884966184520991 1101 14-15 0.8474668056780743 1101 16-17 0.8659760083901418 1101 18-19 0.8294208145705113 1101 20-21 0.6806855480330825 1101 22-23 0.8519088690388656 1101 24-25 0.7479351537241357 1101 26-27 0.8849937271691104 1101 28-29 0.8162644781540038 1101 30-31 0.8715713389191748 1101 32-33 0.8277084555767189 1101 34-35 0.8187119305619461 1101 36-37 0.8521878938041283 1101 38-39 0.9162303210977427 1101 40-41 0.9126474577588013 1101 42-43 0.9364105230184236 1101 44-45 0.9910643729710031 1101 46-47 1.0070672662541256 1101 48-49 0.976668509782975 1101 50-51 0.9652593775466727 1101 52-53 1.1099918539912395 1101 54-55 1.070961359124965 1101 56-57 1.1365432793167685 1101 58-59 0.9717783793149479 1101 60-61 1.043693546117801 1101 62-63 0.9195626017284226 1101 64-65 1.0134377817844182 1101 66-67 1.280774501819316 1101 68-69 1.195773520377827 1101 70-71 1.0407010599712194 1101 72-73 1.0870944153493234 1101 74-75 1.0374593819229325 1101 76-77 1.1544205641258927 1101 78-79 1.1716791906305843 1101 80-81 1.0422866969469595 1101 82-83 1.229077518787399 1101 84-85 1.1969656231056511 1101 86-87 1.1842102121339515 1101 88-89 1.2020273847531833 1101 90-91 1.231133703241408 1101 92-93 1.3493493880795349 1101 94-95 1.288471100535812 1101 96-97 1.351807862941552 1101 98-99 1.350106374866197 1101 100-101 1.546765170169774 1102 1 0.4275993675618146 1102 2 0.4722550552845206 1102 3 0.4623161456885363 1102 4 0.21270632342450568 1102 5 0.35983959161352175 1102 6 0.37278457382941355 1102 7 0.46103722471082875 1102 8 0.5067961912252201 1102 9 0.6389162022122363 1102 10-11 0.6378793388321142 1102 12-13 0.5488794484738193 1102 14-15 0.7300835938232595 1102 16-17 0.6640524427728494 1102 18-19 0.5974971251506744 1102 20-21 0.6901176723151892 1102 22-23 0.6728768810698611 1102 24-25 0.6856155633411234 1102 26-27 0.7420088024084706 1102 28-29 0.7506862746982179 1102 30-31 0.757872555702626 1102 32-33 0.7542049350235374 1102 34-35 0.6520694814089438 1102 36-37 0.808110118444489 1102 38-39 0.9079484201163126 1102 40-41 0.8124771966918374 1102 42-43 0.8574562700103456 1102 44-45 0.9663131509096488 1102 46-47 0.9998513702691127 1102 48-49 0.9390489914660591 1102 50-51 1.033741239171924 1102 52-53 1.0699379868561252 1102 54-55 0.9796258751026627 1102 56-57 0.9034714842712148 1102 58-59 0.9390449887283765 1102 60-61 0.9811450839407811 1102 62-63 0.7633145157807846 1102 64-65 0.7952030691170648 1102 66-67 0.8830702569561382 1102 68-69 1.073917290308401 1102 70-71 0.8770127673906316 1102 72-73 0.946285483061498 1102 74-75 1.0817463184096248 1102 76-77 1.1785975704763096 1102 78-79 1.1831784914720487 1102 80-81 1.1570122267240777 1102 82-83 1.206974856511593 1102 84-85 1.2008100176526035 1102 86-87 1.2184779396797545 1102 88-89 1.2023673530336687 1102 90-91 1.1681110447709067 1102 92-93 1.2796353654223687 1102 94-95 1.2907593297816184 1102 96-97 1.2436671620106878 1102 98-99 1.3404070977426947 1102 100-101 1.5350987359043593 1103 1 0.32624072293237205 1103 2 0.4090537131989471 1103 3 0.36325556155799177 1103 4 0.24876874021642692 1103 5 0.3356625830062683 1103 6 0.3679988021003311 1103 7 0.3033447646011709 1103 8 0.30660891505947774 1103 9 0.5176334587618712 1103 10-11 0.4202209608144969 1103 12-13 0.5183396263806728 1103 14-15 0.5317643616350978 1103 16-17 0.5813935017787557 1103 18-19 0.6163431948043865 1103 20-21 0.5820422309526521 1103 22-23 0.5758899531317994 1103 24-25 0.5237263731497421 1103 26-27 0.5967396446181183 1103 28-29 0.6011966018567421 1103 30-31 0.7028139684087265 1103 32-33 0.7090585469893327 1103 34-35 0.6217668013579072 1103 36-37 0.6194450284264335 1103 38-39 0.6381361303086095 1103 40-41 0.7284897963567474 1103 42-43 0.7768659923308547 1103 44-45 0.8033017426161493 1103 46-47 0.7846642309160785 1103 48-49 0.7648446124526913 1103 50-51 0.7980645870640188 1103 52-53 0.680640035807393 1103 54-55 0.7816445074221008 1103 56-57 0.7234743840717854 1103 58-59 0.649050071242165 1103 60-61 0.614141747912079 1103 62-63 0.523817368561815 1103 64-65 0.49916865647293207 1103 66-67 0.3967088336132534 1103 68-69 0.5159268222233493 1103 70-71 0.5296024669970905 1103 72-73 0.5241002710379554 1103 74-75 0.7507864498253163 1103 76-77 0.8350798277540363 1103 78-79 0.8832366997790047 1103 80-81 0.841688164575249 1103 82-83 0.6919396500391883 1103 84-85 0.7794223687272428 1103 86-87 0.9359934899389302 1103 88-89 0.9427260272272342 1103 90-91 0.9150732913803878 1103 92-93 1.0360595087467424 1103 94-95 1.0791365548360226 1103 96-97 1.0215676387794161 1103 98-99 1.0768829641367397 1103 100-101 1.2430171492920046 1104 1 0.38073389379924194 1104 2 0.5012824908483218 1104 3 0.4743977602617768 1104 4 0.2220185425398853 1104 5 0.3859345994421446 1104 6 0.368853035081564 1104 7 0.4739541489992547 1104 8 0.4582994718652955 1104 9 0.6256601400261914 1104 10-11 0.5905022359464525 1104 12-13 0.5505206913334675 1104 14-15 0.6980451366622944 1104 16-17 0.6955478178855117 1104 18-19 0.7191998742439978 1104 20-21 0.7121299520293647 1104 22-23 0.6500240623033093 1104 24-25 0.6331212464002149 1104 26-27 0.7498443489818669 1104 28-29 0.6787976534283544 1104 30-31 0.8307586730428014 1104 32-33 0.896899243351406 1104 34-35 0.9234877699391646 1104 36-37 0.7813681339417826 1104 38-39 0.7119674733601897 1104 40-41 0.8746393276775066 1104 42-43 0.8164148167467715 1104 44-45 0.9019112486613423 1104 46-47 0.9495649871555685 1104 48-49 0.9456206533801819 1104 50-51 0.8388569151868026 1104 52-53 0.9734209561353069 1104 54-55 1.0122117208249009 1104 56-57 1.121312444746657 1104 58-59 1.1146604355752388 1104 60-61 0.9910669822517022 1104 62-63 0.8688420020999175 1104 64-65 1.0050151513468606 1104 66-67 0.9468923179917326 1104 68-69 0.9258900577659261 1104 70-71 0.9738613996113514 1104 72-73 0.9290158276748137 1104 74-75 1.0689149091647714 1104 76-77 1.1963389428389561 1104 78-79 1.2891713888017051 1104 80-81 1.189917523427443 1104 82-83 1.3240416443758392 1104 84-85 1.378339709521601 1104 86-87 1.1919842717918847 1104 88-89 1.1411588070662582 1104 90-91 1.16779413155637 1104 92-93 1.2701794933381088 1104 94-95 1.2316968884194104 1104 96-97 1.3064888046993026 1104 98-99 1.4297464017486732 1104 100-101 1.6629902517661712 1105 1 0.23426372061742384 1105 2 0.25071254813995125 1105 3 0.3671335513422527 1105 4 0.1698578264054973 1105 5 0.24004563933336698 1105 6 0.23270967310608626 1105 7 0.2207748415356292 1105 8 0.3118754080581425 1105 9 0.3855862475506058 1105 10-11 0.32959413824450223 1105 12-13 0.29059796124732173 1105 14-15 0.4000149174004761 1105 16-17 0.32965943047278046 1105 18-19 0.4070866918821565 1105 20-21 0.41560449165148583 1105 22-23 0.41559346535578356 1105 24-25 0.4850176045672683 1105 26-27 0.4564281142234776 1105 28-29 0.44099218799900797 1105 30-31 0.5138625608413676 1105 32-33 0.5774750084696691 1105 34-35 0.6224281721039588 1105 36-37 0.5738024519452125 1105 38-39 0.3500024577468821 1105 40-41 0.45806197956861183 1105 42-43 0.43646402455394906 1105 44-45 0.35021687598746354 1105 46-47 0.4450633024746722 1105 48-49 0.4898036033375561 1105 50-51 0.49706530165971685 1105 52-53 0.4093516863447988 1105 54-55 0.5351457748742021 1105 56-57 0.46844577059088266 1105 58-59 0.4252871779846288 1105 60-61 0.39392653441807823 1105 62-63 0.428005579511904 1105 64-65 0.38879852832991446 1105 66-67 0.29704705606038573 1105 68-69 0.4578080609069417 1105 70-71 0.5650720203331119 1105 72-73 0.4997596130048443 1105 74-75 0.5933006375790875 1105 76-77 0.6835343034135306 1105 78-79 0.6677600087437128 1105 80-81 0.6618721938004803 1105 82-83 0.5562640207854628 1105 84-85 0.4949496572520715 1105 86-87 0.6315599186998035 1105 88-89 0.7608765853873614 1105 90-91 0.6093238100609604 1105 92-93 0.663148841617339 1105 94-95 0.5920114013450863 1105 96-97 0.562742021525704 1105 98-99 0.7206474729105636 1105 100-101 1.0014367329527367 1106 1 0.08915385184901936 1106 2 0.12988413823141798 1106 3 0.158786081148925 1106 4 0.108116210349678 1106 5 0.09953316643964172 1106 6 0.16409820172508205 1106 7 0.1717847028632704 1106 8 0.172388763875162 1106 9 0.2516877958349397 1106 10-11 0.2406058651036318 1106 12-13 0.2461575833134262 1106 14-15 0.21423514481730166 1106 16-17 0.2088686160080826 1106 18-19 0.2927552090089094 1106 20-21 0.1875098365772203 1106 22-23 0.3037472335691902 1106 24-25 0.3641823947252405 1106 26-27 0.27378072566495604 1106 28-29 0.27062501651144544 1106 30-31 0.33251734174644554 1106 32-33 0.2965292409654481 1106 34-35 0.29818890131315356 1106 36-37 0.49217832877171475 1106 38-39 0.45310272899658344 1106 40-41 0.38869870611622304 1106 42-43 0.4003010169754049 1106 44-45 0.31650996166555245 1106 46-47 0.3318757393038254 1106 48-49 0.5033383547359094 1106 50-51 0.507956196192751 1106 52-53 0.3581351212795809 1106 54-55 0.40543202359297936 1106 56-57 0.49514875341197495 1106 58-59 0.6605685083221928 1106 60-61 0.4179473528950979 1106 62-63 0.4015273861153901 1106 64-65 0.4564241161592406 1106 66-67 0.3512105556550509 1106 68-69 0.4344512149937998 1106 70-71 0.337781537498671 1106 72-73 0.20608832179003045 1106 74-75 0.48688815788543494 1106 76-77 0.491707859077394 1106 78-79 0.47658756848255734 1106 80-81 0.443856855856243 1106 82-83 0.5782521575716437 1106 84-85 0.5204793845320133 1106 86-87 0.6028849195631452 1106 88-89 0.6503590107634345 1106 90-91 0.5795930767468711 1106 92-93 0.5946826253602318 1106 94-95 0.5850213901269754 1106 96-97 0.6448650675683716 1106 98-99 0.7114344886689636 1106 100-101 0.9594910761699467 1107 1 0.10422423619027654 1107 2 0.1490588616462034 1107 3 0.10511941368232769 1107 4 0.06232533485904668 1107 5 0.0530126839981051 1107 6 0.11520411403717645 1107 7 0.13836066861884433 1107 8 0.046213797395189715 1107 9 0.1881121595372548 1107 10-11 0.17237393634881926 1107 12-13 0.06132269108318411 1107 14-15 0.0567135115015347 1107 16-17 0.17137682245783736 1107 18-19 0.22444508114428174 1107 20-21 0.23613391278381357 1107 22-23 0.2725086261784284 1107 24-25 0.17420459970701074 1107 26-27 0.17251507255840437 1107 28-29 0.2995082913982898 1107 30-31 0.25917305619524456 1107 32-33 0.3539136744401503 1107 34-35 0.11180775110401697 1107 36-37 0.07568973277999191 1107 38-39 0.07008362626709186 1107 40-41 0.011101671168667337 1107 42-43 0.16601891805781577 1107 44-45 0.11428747508470138 1107 46-47 0.046082676569355385 1107 48-49 0.13615949132790917 1107 50-51 0.023945120099639894 1107 52-53 -0.0483879392299329 1107 54-55 0.1500650654423552 1107 56-57 -0.059490551908410794 1107 58-59 -0.11092453929355628 1107 60-61 -0.21764452111691668 1107 62-63 -0.2895703943120722 1107 64-65 -0.3631414999561571 1107 66-67 -0.2021494862796871 1107 68-69 -0.05200271763978037 1107 70-71 -0.16189352994420148 1107 72-73 -0.21056424892427827 1107 74-75 -0.1380758365840613 1107 76-77 -0.059062870114736654 1107 78-79 0.04119206645695428 1107 80-81 0.14916887578964477 1107 82-83 0.09497541945843935 1107 84-85 0.06702907593522056 1107 86-87 0.08051568880446425 1107 88-89 0.22949685272169518 1107 90-91 0.18190949825138958 1107 92-93 0.2955722987923828 1107 94-95 0.287445391117064 1107 96-97 0.2162929092626591 1107 98-99 0.08833421108097994 1107 100-101 0.4079557029860368 1108 1 0.2700073269191918 1108 2 0.3323555853207978 1108 3 0.2732500588420841 1108 4 0.10900914147141094 1108 5 0.19534823656965727 1108 6 0.17636682882294252 1108 7 0.30387756496087803 1108 8 0.3319887663403094 1108 9 0.33522629561478823 1108 10-11 0.33600908444901023 1108 12-13 0.23630236962966222 1108 14-15 0.3433364822281746 1108 16-17 0.4903321807890606 1108 18-19 0.44472256772002083 1108 20-21 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-0.8435909915009532 2316 62-63 -1.113075075533807 2316 64-65 -0.992626406038525 2316 66-67 -0.8725916115227719 2316 68-69 -0.9761052709705247 2316 70-71 -0.7321778945418842 2316 72-73 -0.803034519818592 2316 74-75 -0.5491620625473068 2316 76-77 -1.07747904648458 2316 78-79 -0.8849106608019568 2316 80-81 -1.0004474996189465 2316 82-83 -1.1386229593973702 2316 84-85 -0.9953740193814689 2316 86-87 -0.92426944426769 2316 88-89 -1.1914088754266707 2316 90-91 -0.9859002329261592 2316 92-93 -0.9611087985018081 2316 94-95 -0.8253255033964599 2316 96-97 -0.8522139030963025 2316 98-99 -0.7673307112914429 2316 100-101 -0.7981718118215291 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 15.0 4 56.0 5 103.0 6 229.0 7 581.0 8 1500.0 9 2685.0 10 4473.0 11 6766.0 12 9281.0 13 12265.0 14 14455.0 15 17547.0 16 20675.0 17 23767.0 18 21389.0 19 19113.0 20 19651.0 21 21063.0 22 23257.0 23 27421.0 24 33474.0 25 42279.0 26 54074.0 27 69318.0 28 87557.0 29 109319.0 30 133203.0 31 160843.0 32 192124.0 33 231806.0 34 283100.0 35 351044.0 36 451262.0 37 570931.0 38 491314.0 39 88310.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.91649200797423 21.880173012228333 14.891489115537283 23.311845864260157 2 15.229861804563171 22.139605838372766 40.11060195392892 22.51993040313514 3 18.194460549707145 26.99093720949698 31.021043993645396 23.793558247150482 4 12.814104388205582 18.39262224469774 39.20094530688677 29.592328060209905 5 12.60769024140522 38.55064548797979 35.947337657534604 12.894326613080384 6 29.59659535657133 39.27321818609029 18.08829438320035 13.041892074138028 7 25.615415758781047 33.17658750682652 23.744504024727856 17.463492709664575 8 24.326412825740462 37.06000220228303 21.05541077583762 17.558174196138886 9 25.204295119273645 16.316720555865135 21.472621061611648 37.00636326324957 10-11 23.773311666427528 27.161699294935747 29.52897281292718 19.53601622570954 12-13 24.25699363896128 24.665361424676917 29.619344275788123 21.458300660573684 14-15 21.623063807979747 26.34544742443309 26.97562875276346 25.055860014823704 16-17 21.369375141118514 29.234959685985583 27.679263614391363 21.71640155850454 18-19 21.28979264059297 28.337626672984328 29.500387624059165 20.87219306236354 20-21 21.960585251154807 27.535239309194974 29.4366131778833 21.06756226176692 22-23 21.743892621928584 27.419183514006896 29.2519461163179 21.58497774774662 24-25 21.676293827262796 27.610754983873477 29.204512813211764 21.508438375651963 26-27 21.656991617800163 27.7087958596204 29.08185936679688 21.552353155782562 28-29 21.66234667601711 27.818844298342267 29.074035273535838 21.444773752104787 30-31 21.934104749813372 27.605352075506694 28.983123173069824 21.477420001610113 32-33 21.798554468052085 27.728075120713626 28.89033576202206 21.58303464921222 34-35 21.92897878885889 27.751676331185365 28.94018825857619 21.37915662137956 36-37 21.96270303645928 27.571053636647896 28.937187112833957 21.529056214058862 38-39 21.802690666504645 27.690009569699964 28.94068857626973 21.566611187525663 40-41 21.917963444362428 27.544015686603835 29.011207557332558 21.526813311701176 42-43 21.82497781542489 27.65627437626652 28.749109766580172 21.769638041728413 44-45 21.86126025538258 27.772962984334583 28.683925302647197 21.681851457635652 46-47 22.006452467798493 27.738409063321534 28.456598234393688 21.79854023448629 48-49 21.9548065711121 27.79820524421977 28.372105505098695 21.874882679569435 50-51 21.960663360008382 27.855350138557565 28.219603568783675 21.96438293265038 52-53 22.059374412730357 27.86960725631935 28.20596887823865 21.86504945271164 54-55 21.950332602498264 27.7736748359167 28.27900647405753 21.9969860875275 56-57 21.99037931420459 27.943788720340336 28.109241156067434 21.95659080938764 58-59 21.99188502308672 27.815517077384584 28.290851474618517 21.901746424910183 60-61 21.97218859274079 27.965636860304315 28.24047353648461 21.821701010470278 62-63 21.97083963189231 27.895975593089755 28.220935310609995 21.912249464407935 64-65 21.819581669433706 27.79426928681944 28.2383222463908 22.147826797356057 66-67 21.861617338466505 27.872415257380652 28.13741535550306 22.128552048649787 68-69 21.84867271367882 27.87551795147582 28.177256175195225 22.098553159650137 70-71 21.84861553533979 27.63959804113941 28.274836100592697 22.236950322928102 72-73 21.89241595853276 27.779203854218537 28.115243115424683 22.213137071824026 74-75 21.85181437309201 27.969202866734854 27.9553341768842 22.22364858328894 76-77 21.80458247468471 27.8660409136277 28.00661361070037 22.322763000987216 78-79 21.728075296860556 27.881150937852865 28.03473797947807 22.356035785808505 80-81 21.78794593295189 28.009377019905664 27.900652306569768 22.302024740572683 82-83 21.906445793250114 27.80799428741128 28.02811136433658 22.257448555002018 84-85 21.9576655577393 27.87645414635394 27.785721197641557 22.3801590982652 86-87 21.81287657093409 27.955689064330024 27.880597330343566 22.35083703439232 88-89 21.82945439398397 27.813758078536075 27.84951747065978 22.507270056820172 90-91 21.840036328709193 27.814543385611007 27.91082948142603 22.434590804253773 92-93 21.87783893029767 27.74774383912581 27.88496503209638 22.489452198480137 94-95 21.753896608757454 27.68160538167468 27.91592189986997 22.648576109697892 96-97 21.691375614943045 27.59318827184181 27.96586628137836 22.749569831836784 98-99 21.5843062864058 27.65412644441404 27.881709594501856 22.879857674678306 100-101 21.617827147476216 27.631227706055277 27.833701492769723 22.91724365369878 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 341.0 1 242.5 2 191.5 3 267.0 4 246.0 5 229.5 6 367.5 7 697.0 8 1158.5 9 1475.0 10 1372.0 11 1207.0 12 1305.0 13 1511.0 14 1751.5 15 2012.5 16 2298.0 17 2633.0 18 2967.0 19 3392.5 20 3995.0 21 4775.5 22 5823.0 23 7449.5 24 10029.5 25 13898.0 26 19640.0 27 27182.5 28 36271.5 29 48187.5 30 61079.0 31 72810.0 32 85163.0 33 94752.5 34 101382.5 35 106796.5 36 113714.5 37 120172.0 38 120850.5 39 119025.5 40 117404.0 41 116701.0 42 116921.0 43 118875.0 44 121115.0 45 123872.0 46 125724.0 47 126410.5 48 127294.5 49 125822.5 50 121364.0 51 116867.0 52 113677.5 53 110702.5 54 106500.0 55 98136.0 56 87931.0 57 77952.5 58 68411.5 59 59655.0 60 50399.0 61 41011.5 62 32561.5 63 24914.0 64 18710.5 65 13895.0 66 9949.5 67 7580.5 68 5540.5 69 3735.0 70 2660.5 71 1842.5 72 1233.0 73 841.0 74 577.0 75 378.0 76 252.5 77 144.5 78 81.0 79 50.5 80 27.5 81 16.5 82 12.5 83 7.0 84 6.0 85 4.0 86 2.5 87 2.0 88 1.0 89 1.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01901971710673398 2 0.0035870519104805316 3 0.003753891534223812 4 0.03461922192673071 5 0.002057688692833793 6 8.341981187164026E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 4.170990593582013E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.341981187164026E-5 24-25 5.28325475187055E-4 26-27 0.00211330190074822 28-29 6.951650989303355E-5 30-31 0.006743101459624255 32-33 0.021550118066840405 34-35 0.013041297255933094 36-37 6.534551929945155E-4 38-39 5.978419850800886E-4 40-41 0.0021828184106412536 42-43 0.00836978779112124 44-45 0.0080778184495705 46-47 0.007771945806041152 48-49 0.009648891573153058 50-51 0.19870599187824714 52-53 0.04931501211811801 54-55 0.01694812511192158 56-57 0.010302346766147573 58-59 0.019297783146306115 60-61 0.054264587622502 62-63 0.09774021290960518 64-65 0.10228659265660957 66-67 0.10612390400270504 68-69 0.10427476483955034 70-71 0.09351360910810874 72-73 0.06928015375939724 74-75 0.25167757241673866 76-77 0.30405131097015015 78-79 0.00727142693481131 80-81 8.202948167377959E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 2.780660395721342E-4 90-91 0.005950613246843672 92-93 0.017059351527750434 94-95 0.001779622653261659 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3596268.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.45075140923376 #Duplication Level Percentage of deduplicated Percentage of total 1 78.67150088101697 47.55751342749671 2 12.345918774084968 14.926401334616045 3 3.8205220339625 6.928602831857017 4 1.6439117181784497 3.975027944573273 5 0.905852452397102 2.7379730706650984 6 0.5811966295250769 2.1080263782782973 7 0.37365643235737245 1.5811468473427686 8 0.2823519203537997 1.365470859778185 9 0.19511416451482824 1.06153180699556 >10 1.0935755219749639 11.800739879881023 >50 0.059945266320933596 2.464022930509244 >100 0.02539438702341606 2.702401134655683 >500 7.833439586668144E-4 0.3096655434584395 >1k 1.8431622061040558E-4 0.1744567110187083 >5k 9.215811030520213E-5 0.30701929887405305 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5984 0.16639471807996511 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5038 0.14008967073644124 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.780660395721342E-5 2 0.0 0.0 0.0 0.0 2.780660395721342E-5 3 0.0 0.0 0.0 0.0 2.780660395721342E-5 4 0.0 0.0 0.0 0.0 5.561320791442684E-5 5 0.0 0.0 0.0 0.0 5.561320791442684E-5 6 0.0 0.0 0.0 0.0 5.561320791442684E-5 7 0.0 0.0 0.0 0.0 5.561320791442684E-5 8 0.0 0.0 0.0 0.0 5.561320791442684E-5 9 0.0 0.0 0.0 0.0 5.561320791442684E-5 10-11 0.0 0.0 0.0 1.390330197860671E-5 5.561320791442684E-5 12-13 0.0 0.0 0.0 5.561320791442684E-5 9.732311385024698E-5 14-15 0.0 0.0 0.0 8.341981187164026E-5 1.2512971780746042E-4 16-17 2.780660395721342E-5 0.0 0.0 8.341981187164026E-5 1.3903301978606712E-4 18-19 2.780660395721342E-5 0.0 0.0 8.341981187164026E-5 1.3903301978606712E-4 20-21 2.780660395721342E-5 0.0 0.0 8.341981187164026E-5 2.2245283165770737E-4 22-23 2.780660395721342E-5 0.0 0.0 1.3903301978606712E-4 2.2245283165770737E-4 24-25 4.170990593582013E-5 0.0 0.0 3.8929245540098793E-4 2.2245283165770737E-4 26-27 5.561320791442684E-5 0.0 0.0 6.395518910159088E-4 2.502594356149208E-4 28-29 5.561320791442684E-5 0.0 0.0 0.0012930070840104241 2.502594356149208E-4 30-31 6.951650989303355E-5 1.390330197860671E-5 0.0 0.004087570781710373 2.641627375935275E-4 32-33 1.1122641582885369E-4 2.780660395721342E-5 0.0 0.012999587349997274 3.0587264352934765E-4 34-35 1.2512971780746042E-4 2.780660395721342E-5 0.0 0.02765366763544875 3.8929245540098793E-4 36-37 1.3903301978606712E-4 2.780660395721342E-5 0.0 0.04969040127154038 3.8929245540098793E-4 38-39 1.3903301978606712E-4 2.780660395721342E-5 0.0 0.08323906894591838 4.866155692512349E-4 40-41 1.3903301978606712E-4 2.780660395721342E-5 0.0 0.11920691116457394 5.005188712298416E-4 42-43 1.3903301978606712E-4 2.780660395721342E-5 0.0 0.1576495411354215 5.005188712298416E-4 44-45 1.3903301978606712E-4 2.780660395721342E-5 0.0 0.2049068645607057 5.005188712298416E-4 46-47 1.3903301978606712E-4 2.780660395721342E-5 0.0 0.2620355323908007 5.144221732084483E-4 48-49 1.3903301978606712E-4 2.780660395721342E-5 0.0 0.3233629974184349 5.839386831014818E-4 50-51 1.3903301978606712E-4 2.780660395721342E-5 0.0 0.3872764766140899 6.395518910159088E-4 52-53 1.3903301978606712E-4 2.780660395721342E-5 0.0 0.45359522705204397 6.534551929945154E-4 54-55 1.3903301978606712E-4 5.561320791442684E-5 0.0 0.528923317172135 6.673584949731221E-4 56-57 1.3903301978606712E-4 5.561320791442684E-5 0.0 0.6170424451125445 7.090684009089423E-4 58-59 1.3903301978606712E-4 5.561320791442684E-5 0.0 0.7180499339871222 7.507783068447624E-4 60-61 1.6683962374328053E-4 5.561320791442684E-5 0.0 0.826620819138062 8.063915147591892E-4 62-63 1.6683962374328053E-4 5.561320791442684E-5 0.0 0.9395851477142416 8.620047226736161E-4 64-65 1.6683962374328053E-4 5.561320791442684E-5 0.0 1.0582220234976925 8.620047226736161E-4 66-67 1.6683962374328053E-4 5.561320791442684E-5 0.0 1.1925001140070761 9.17617930588043E-4 68-69 1.6683962374328053E-4 5.561320791442684E-5 0.0 1.3477582872021774 9.732311385024698E-4 70-71 2.0854952967910067E-4 5.561320791442684E-5 0.0 1.5161689840690404 0.00101494104443829 72-73 2.2245283165770737E-4 5.561320791442684E-5 0.0 1.7004989617014084 0.0010705542523527169 74-75 2.2245283165770737E-4 9.732311385024698E-5 0.0 1.8873454369919038 0.0011261674602671436 76-77 2.2245283165770737E-4 1.1122641582885369E-4 0.0 2.095714223745283 0.001153974064224357 78-79 2.2245283165770737E-4 1.1122641582885369E-4 0.0 2.334072432866516 0.0011817806681815703 80-81 2.2245283165770737E-4 1.1122641582885369E-4 0.0 2.5962609015790816 0.001209587272138784 82-83 2.502594356149208E-4 1.1122641582885369E-4 0.0 2.878845514294263 0.0013625235939034577 84-85 2.502594356149208E-4 1.1122641582885369E-4 0.0 3.1678117426176247 0.0013903301978606712 86-87 2.502594356149208E-4 1.1122641582885369E-4 0.0 3.477758053626704 0.0014737500097323115 88-89 2.502594356149208E-4 1.1122641582885369E-4 0.0 3.8214754851418196 0.0015571698216039517 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4290 0.0 50.36511 1 GTATCAA 7725 0.0 41.555054 1 TCAACGC 9510 0.0 33.343292 4 ATCAACG 9570 0.0 33.135166 3 CAACGCA 9560 0.0 33.0581 5 TATCAAC 9855 0.0 32.659973 2 AACGCAG 10135 0.0 31.416685 6 ACGCAGA 11880 0.0 26.60231 7 CGCAGAG 12185 0.0 25.897491 8 GTACATG 9855 0.0 23.659225 1 GCAGAGT 13875 0.0 22.059433 9 TACATGG 10260 0.0 22.024311 2 ACATGGG 10035 0.0 21.523785 3 AGAGTAC 12740 0.0 19.648104 10-11 GTGGTAT 2135 0.0 19.57314 1 CATGGGG 6515 0.0 19.454021 4 GAGTACT 8680 0.0 19.407797 12-13 CAGAGTA 13410 0.0 18.82567 10-11 ATAACGC 320 8.35098E-9 17.801472 3 GTACTTT 9425 0.0 17.72266 14-15 >>END_MODULE