FastQCFastQC Report
Sat 18 Jun 2016
SRR1930954_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930954_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3703307
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN67500.1822695228885966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28800.057.6194421
GTATCAA55900.044.4836851
ATCAACG69650.035.5363733
TCAACGC71100.034.9394724
TATCAAC72850.033.8444062
CAACGCA73650.032.7669755
AACGCAG81600.030.1114736
ACGCAGA90800.026.6744827
CGCAGAG94550.025.5802388
GTACATG76450.025.2029571
TACATGG75600.024.3432542
ACATGGG79600.022.9882723
GCAGAGT101600.022.4431699
AGAGTAC90500.019.65313110-11
GTGGTAT16200.019.3037761
CAGAGTA97050.019.1690710-11
GAGTACT59500.018.80486512-13
GTGTAGC14700.017.842331
AGTACTT64250.017.5930512-13
CATGGGG56550.017.4647524
Produced by FastQC (version 0.11.5)