##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930954_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3703307 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.089105494089473 30.0 10.0 31.0 2.0 33.0 2 21.909695577493306 30.0 10.0 31.0 2.0 33.0 3 22.384522806237776 30.0 10.0 31.0 2.0 33.0 4 24.327774337909332 33.0 2.0 35.0 2.0 37.0 5 24.309758818267024 33.0 10.0 35.0 2.0 37.0 6 25.112813763482208 33.0 17.0 35.0 2.0 37.0 7 25.21366848603154 32.0 17.0 35.0 2.0 37.0 8 26.05257139092168 33.0 17.0 35.0 2.0 37.0 9 27.261478726986446 34.0 17.0 37.0 2.0 39.0 10-11 27.559690973500174 34.0 20.0 37.0 2.0 39.0 12-13 27.52962392801893 34.0 19.0 37.0 2.0 39.0 14-15 27.775197681423656 34.0 17.5 38.0 2.0 40.0 16-17 26.864290349139296 33.5 14.0 38.0 2.0 40.0 18-19 26.202075604317976 33.0 10.5 38.0 2.0 40.0 20-21 25.678353833479104 32.5 10.0 38.0 2.0 40.0 22-23 25.606951840611647 32.0 10.0 38.0 2.0 40.0 24-25 25.31971235439028 32.0 10.0 38.0 2.0 40.0 26-27 24.998873439334087 32.0 9.0 38.0 2.0 40.0 28-29 24.93928588691135 32.0 8.5 38.0 2.0 40.0 30-31 24.87256754570982 32.0 8.0 38.0 2.0 40.0 32-33 24.589625029736936 31.0 5.0 38.0 2.0 40.0 34-35 21.63785003511726 26.5 2.0 36.5 2.0 39.0 36-37 19.96206525680966 19.0 2.0 35.0 2.0 38.5 38-39 19.02939116308748 19.5 2.0 34.0 2.0 38.0 40-41 20.431748839618212 24.5 2.0 35.0 2.0 38.0 42-43 20.360168222618327 24.5 2.0 35.0 2.0 38.0 44-45 18.327403048140486 19.0 2.0 34.0 2.0 38.0 46-47 19.214638429922232 19.0 2.0 33.0 2.0 38.0 48-49 18.644088648335124 19.5 2.0 34.0 2.0 38.0 50-51 16.591869915186617 11.5 2.0 32.0 2.0 37.5 52-53 19.60707375867029 23.0 2.0 33.5 2.0 38.0 54-55 21.287419325483953 27.5 2.0 36.0 2.0 39.0 56-57 21.51678796815927 28.0 2.0 36.0 2.0 39.0 58-59 21.135033228409092 28.0 2.0 36.0 2.0 39.0 60-61 20.827529151647433 27.0 2.0 36.0 2.0 39.0 62-63 20.35397861964995 26.0 2.0 35.0 2.0 39.0 64-65 19.848408463030474 26.0 2.0 34.5 2.0 38.0 66-67 19.41912795779556 25.0 2.0 34.0 2.0 38.0 68-69 18.95042363487553 23.5 2.0 34.0 2.0 37.5 70-71 18.35837955103371 22.0 2.0 34.0 2.0 37.0 72-73 17.88596665088798 20.5 2.0 33.0 2.0 36.0 74-75 17.336020346139275 18.5 2.0 32.5 2.0 36.0 76-77 16.769460241886506 15.5 2.0 32.0 2.0 35.0 78-79 16.26528424459544 9.5 2.0 31.5 2.0 35.0 80-81 15.758737663391127 7.0 2.0 31.0 2.0 34.5 82-83 15.272419623865911 4.0 2.0 31.0 2.0 34.0 84-85 14.745710118010741 2.0 2.0 30.5 2.0 34.0 86-87 14.180158976828007 2.0 2.0 30.0 2.0 34.0 88-89 13.615951364550657 2.0 2.0 29.5 2.0 34.0 90-91 13.032635290565972 2.0 2.0 29.0 2.0 34.0 92-93 12.397684555992793 2.0 2.0 29.0 2.0 34.0 94-95 11.736724635575715 2.0 2.0 27.5 2.0 34.0 96-97 10.994099192964558 2.0 2.0 26.0 2.0 33.0 98-99 10.174728155132696 2.0 2.0 25.0 2.0 33.0 100-101 9.080922807641926 2.0 2.0 18.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.726746455065065 1101 2 7.811717217627443 1101 3 7.348686326881488 1101 4 9.370105044752375 1101 5 9.192569235837578 1101 6 8.466277700477075 1101 7 8.25263631948128 1101 8 7.2039216697688175 1101 9 7.5842261753911195 1101 10-11 6.779693837111953 1101 12-13 6.546722315560036 1101 14-15 7.062919083373874 1101 16-17 7.762220571964249 1101 18-19 8.236139183114002 1101 20-21 8.815336116913201 1101 22-23 8.727322263883028 1101 24-25 8.785498626288113 1101 26-27 8.70891029684439 1101 28-29 8.553600259477516 1101 30-31 8.359060191381825 1101 32-33 8.37559289816355 1101 34-35 10.816856758918323 1101 36-37 5.0814699014984015 1101 38-39 8.115127848658535 1101 40-41 9.713583542075668 1101 42-43 10.100030035084409 1101 44-45 4.330264873361674 1101 46-47 5.966658030063819 1101 48-49 9.042820529578304 1101 50-51 -3.7908706752651042 1101 52-53 5.759968265552853 1101 54-55 7.978974877778533 1101 56-57 8.505555619864513 1101 58-59 8.817186713025134 1101 60-61 8.566093250904707 1101 62-63 8.647181507772377 1101 64-65 8.670837290439295 1101 66-67 8.529208108173325 1101 68-69 8.499737164274364 1101 70-71 8.440731187601141 1101 72-73 8.334418673529594 1101 74-75 8.350740272495496 1101 76-77 8.132602171552435 1101 78-79 8.009797508413577 1101 80-81 7.889963617673169 1101 82-83 7.969621773467871 1101 84-85 8.005432220023794 1101 86-87 7.7396225477246645 1101 88-89 7.639261474242122 1101 90-91 7.512933711853206 1101 92-93 7.405448003337378 1101 94-95 7.273408869512648 1101 96-97 6.837999256922638 1101 98-99 6.777968549681644 1101 100-101 6.369127467485496 1102 1 6.507757950037522 1102 2 7.593800797105139 1102 3 7.464492567355315 1102 4 9.07199711665719 1102 5 9.107120217582615 1102 6 8.494983534574619 1102 7 8.047365653210509 1102 8 7.045750890147367 1102 9 7.072140382644353 1102 10-11 6.609956216543043 1102 12-13 6.1700308220877 1102 14-15 6.768927481393778 1102 16-17 7.490314708100527 1102 18-19 7.7038304378367535 1102 20-21 8.245462739823388 1102 22-23 8.196658447052524 1102 24-25 8.137261534231332 1102 26-27 8.161021997684824 1102 28-29 8.045361701259164 1102 30-31 7.9543897433077895 1102 32-33 7.918082201311574 1102 34-35 10.24541412385539 1102 36-37 0.8555242531264682 1102 38-39 3.306223589599451 1102 40-41 8.167255051308128 1102 42-43 9.188275760219426 1102 44-45 1.0054994924335574 1102 46-47 1.729149822585864 1102 48-49 7.5555863230396945 1102 50-51 -4.926912603056426 1102 52-53 4.741753201355504 1102 54-55 7.41041392200065 1102 56-57 7.989926972455404 1102 58-59 8.295599108099719 1102 60-61 8.191886480486563 1102 62-63 8.366069869387765 1102 64-65 8.098689849511356 1102 66-67 7.997433781001956 1102 68-69 8.018676487185303 1102 70-71 7.949408966778311 1102 72-73 7.974150330526928 1102 74-75 7.754890587572582 1102 76-77 7.698684421001591 1102 78-79 7.545318815963924 1102 80-81 7.588183419949546 1102 82-83 7.369415462535606 1102 84-85 7.242576950841405 1102 86-87 6.99615290959785 1102 88-89 7.012352123630624 1102 90-91 6.911109381632867 1102 92-93 6.777473433931904 1102 94-95 6.515316890538555 1102 96-97 6.250521014952236 1102 98-99 5.863727337016899 1102 100-101 5.399799589611883 1103 1 6.683126782427031 1103 2 7.823205488047254 1103 3 7.521162016043814 1103 4 9.143062151976569 1103 5 9.211333640055042 1103 6 8.427407502118125 1103 7 8.220324043501652 1103 8 7.318256613812309 1103 9 7.297924941758243 1103 10-11 6.80185680791752 1103 12-13 6.756019856588853 1103 14-15 7.035843171546464 1103 16-17 7.813176814410653 1103 18-19 8.326963324745318 1103 20-21 8.680218929678972 1103 22-23 8.523146548099906 1103 24-25 8.561321811800038 1103 26-27 8.480105459126289 1103 28-29 8.28494272205025 1103 30-31 8.25952284171007 1103 32-33 8.165812701401268 1103 34-35 10.42282153261333 1103 36-37 -3.25504956765484 1103 38-39 -1.4816675155882173 1103 40-41 6.945663830628305 1103 42-43 9.336088026440393 1103 44-45 0.9249923851220885 1103 46-47 0.7226098190638268 1103 48-49 7.805641323561279 1103 50-51 -5.1951587803146175 1103 52-53 4.604120911426929 1103 54-55 7.828653546385521 1103 56-57 8.30292641454956 1103 58-59 8.330802873860488 1103 60-61 8.42798933049669 1103 62-63 8.231399957243458 1103 64-65 8.199671021636998 1103 66-67 8.166709477935605 1103 68-69 8.203065762609107 1103 70-71 8.214934253755192 1103 72-73 7.828739461794672 1103 74-75 7.935038012565062 1103 76-77 7.915181560223729 1103 78-79 7.581522350711779 1103 80-81 7.528221161353928 1103 82-83 7.449878812526038 1103 84-85 7.308097390193508 1103 86-87 7.220185602894567 1103 88-89 7.055444254741573 1103 90-91 6.8873337125480365 1103 92-93 6.839340923465 1103 94-95 6.624389735109764 1103 96-97 6.332283964527429 1103 98-99 6.0304276439115885 1103 100-101 5.5782227831084 1104 1 6.264641903746142 1104 2 7.456932158018944 1104 3 7.005657890068036 1104 4 8.960003633314404 1104 5 8.763117795696115 1104 6 7.853996929316391 1104 7 7.759001181503837 1104 8 7.012597839375893 1104 9 7.042160821353324 1104 10-11 6.273125688289792 1104 12-13 6.176966965566624 1104 14-15 6.69367120326314 1104 16-17 7.306667305984284 1104 18-19 8.000442334180352 1104 20-21 8.088192469351753 1104 22-23 8.127716428235527 1104 24-25 8.125341074393415 1104 26-27 8.10469496681392 1104 28-29 8.01111452797662 1104 30-31 7.561139437514395 1104 32-33 7.49110953694262 1104 34-35 10.106372738527316 1104 36-37 -3.466800787433943 1104 38-39 -1.911484648989827 1104 40-41 6.333599102018734 1104 42-43 8.57638437201652 1104 44-45 0.39085807235447945 1104 46-47 -0.2097211253617104 1104 48-49 7.0515717523587185 1104 50-51 -5.477720346064295 1104 52-53 3.8174304066772997 1104 54-55 6.590018637734747 1104 56-57 7.2245005549706995 1104 58-59 7.319967337751013 1104 60-61 7.4883702395220695 1104 62-63 7.5185854233450975 1104 64-65 7.565424581947585 1104 66-67 7.210696329777427 1104 68-69 7.5045225511909734 1104 70-71 7.403242739442906 1104 72-73 7.177700204898944 1104 74-75 7.155426058130313 1104 76-77 7.305496918655688 1104 78-79 7.0564052586712265 1104 80-81 6.799903225057124 1104 82-83 6.730901949497021 1104 84-85 6.519267818566449 1104 86-87 6.4521824130900285 1104 88-89 6.540036881392069 1104 90-91 6.220659947932292 1104 92-93 6.02054298388247 1104 94-95 5.831338847776545 1104 96-97 5.634419089631189 1104 98-99 5.401029000074681 1104 100-101 4.787089823637139 1105 1 5.764977314783078 1105 2 6.953970808760204 1105 3 6.672517862577433 1105 4 8.475892277384876 1105 5 8.44716273783403 1105 6 7.515368940929715 1105 7 7.317980852841121 1105 8 6.415736372288826 1105 9 6.367631579900955 1105 10-11 5.8867071081492774 1105 12-13 5.48067363852174 1105 14-15 5.996468630236709 1105 16-17 6.540162649933663 1105 18-19 7.366873812071699 1105 20-21 7.4515547825195085 1105 22-23 7.305751443597064 1105 24-25 7.491427516060636 1105 26-27 7.469476594042728 1105 28-29 7.073672496334883 1105 30-31 7.098700995265386 1105 32-33 6.966845776868592 1105 34-35 9.149854588498986 1105 36-37 -3.945801168965671 1105 38-39 -2.641822463649895 1105 40-41 5.177560379890668 1105 42-43 7.301597376072198 1105 44-45 -3.654786570547067 1105 46-47 -4.610371146336597 1105 48-49 4.7032161754851565 1105 50-51 -10.051163599035338 1105 52-53 0.2953287042757857 1105 54-55 4.815427153405221 1105 56-57 5.898853000450028 1105 58-59 6.288267117949172 1105 60-61 6.128418250490505 1105 62-63 6.158174433199417 1105 64-65 6.174942033882477 1105 66-67 5.889307185330903 1105 68-69 5.854132677636528 1105 70-71 6.02717517918455 1105 72-73 5.979323158510624 1105 74-75 5.647574111155468 1105 76-77 5.60169026406863 1105 78-79 5.361388873126867 1105 80-81 5.297749789948133 1105 82-83 5.194985028765515 1105 84-85 5.094427696936167 1105 86-87 4.818002023179048 1105 88-89 4.788738751111417 1105 90-91 4.625687824880691 1105 92-93 4.606654988442669 1105 94-95 4.442411636383104 1105 96-97 4.213523222659834 1105 98-99 3.7890898777794604 1105 100-101 3.5702723222662183 1106 1 5.8019402975994865 1106 2 7.059711915521856 1106 3 6.632333240218731 1106 4 8.180468430574418 1106 5 7.941602490496972 1106 6 7.247849156674729 1106 7 6.899546274925761 1106 8 6.057690144215336 1106 9 6.192412277475288 1106 10-11 5.651437011299752 1106 12-13 5.484217078950881 1106 14-15 5.8854598371336415 1106 16-17 6.44682700463909 1106 18-19 6.7019295111469255 1106 20-21 7.249942653112441 1106 22-23 7.023243167929845 1106 24-25 7.179580640249004 1106 26-27 7.181621437960704 1106 28-29 7.169366556046757 1106 30-31 6.90941007624199 1106 32-33 6.801489167544528 1106 34-35 9.213107540009911 1106 36-37 -0.23145360818851302 1106 38-39 1.1395304473141792 1106 40-41 6.904107912478235 1106 42-43 7.818597163548112 1106 44-45 -3.5789537879739903 1106 46-47 -4.332362118406731 1106 48-49 4.853927910743682 1106 50-51 -9.949005643198078 1106 52-53 0.3892624058372469 1106 54-55 4.93198332440322 1106 56-57 5.931800490403113 1106 58-59 6.373261290069305 1106 60-61 6.356870752264673 1106 62-63 6.378947962526897 1106 64-65 6.306007544594454 1106 66-67 6.233984786031211 1106 68-69 5.882559070975688 1106 70-71 6.012464174988143 1106 72-73 5.95104004712168 1106 74-75 5.625290980057152 1106 76-77 5.469528192603228 1106 78-79 5.4234485323596004 1106 80-81 5.226163242528353 1106 82-83 5.2694877852898045 1106 84-85 5.095389987590538 1106 86-87 5.119006961284484 1106 88-89 4.908183836326572 1106 90-91 4.5913717329691455 1106 92-93 4.628373647007228 1106 94-95 4.279776379194596 1106 96-97 4.224835494713487 1106 98-99 3.9985785596075605 1106 100-101 3.720948820538341 1107 1 5.703137887096577 1107 2 7.052066942027757 1107 3 6.699328885800583 1107 4 7.79332472277185 1107 5 8.443748668642332 1107 6 6.926965163436151 1107 7 7.139913821581015 1107 8 6.405033941484028 1107 9 6.41112949467276 1107 10-11 5.946864246519429 1107 12-13 5.536889652731379 1107 14-15 5.8078712160599615 1107 16-17 6.886352227611624 1107 18-19 6.969757864654834 1107 20-21 7.414505058926238 1107 22-23 7.353651485137956 1107 24-25 7.671771892413343 1107 26-27 7.596502102334139 1107 28-29 7.485479735673568 1107 30-31 7.393751237924999 1107 32-33 7.0368951816959076 1107 34-35 9.412613995017555 1107 36-37 -0.06962498881068413 1107 38-39 1.021831319104475 1107 40-41 7.076883138611333 1107 42-43 8.269420134403173 1107 44-45 -3.269996110456331 1107 46-47 -4.029449406108256 1107 48-49 5.154127970838967 1107 50-51 -9.839411812504096 1107 52-53 0.8274340482429672 1107 54-55 5.52524958751458 1107 56-57 6.750160954089456 1107 58-59 7.147856556741818 1107 60-61 7.129137422353683 1107 62-63 7.033802125466341 1107 64-65 6.935320827606606 1107 66-67 6.937405383952498 1107 68-69 6.837460789131349 1107 70-71 6.823286741036451 1107 72-73 6.629055285988951 1107 74-75 6.599297629614664 1107 76-77 6.43062926442169 1107 78-79 6.472867136639543 1107 80-81 6.228421700275918 1107 82-83 6.044350965076234 1107 84-85 5.914278360148627 1107 86-87 5.76633446412356 1107 88-89 5.675925801263666 1107 90-91 5.548127740614246 1107 92-93 5.450671462953471 1107 94-95 5.312603940328399 1107 96-97 5.119709191813579 1107 98-99 5.13707144235139 1107 100-101 4.707568680306641 1108 1 5.974728665870291 1108 2 6.698988447232349 1108 3 6.761277248448771 1108 4 8.188027135538913 1108 5 8.268936711704352 1108 6 7.4825593293399955 1108 7 7.7424728045409275 1108 8 6.694639839472842 1108 9 5.018152260528684 1108 10-11 5.869466710277745 1108 12-13 5.311245209754556 1108 14-15 6.030862936124098 1108 16-17 6.243040299343427 1108 18-19 6.995811082428457 1108 20-21 7.953297835779068 1108 22-23 7.8417585806021926 1108 24-25 7.489608759809453 1108 26-27 7.88820556615595 1108 28-29 7.699539474287054 1108 30-31 7.524352404887576 1108 32-33 7.568231447026559 1108 34-35 10.046198803308858 1108 36-37 -3.4996421113269065 1108 38-39 -2.1126724581394996 1108 40-41 5.880607305088574 1108 42-43 8.272598256696185 1108 44-45 -3.161965358529825 1108 46-47 -4.103817736543892 1108 48-49 5.368817996723685 1108 50-51 -10.003046777557678 1108 52-53 0.8060776193511963 1108 54-55 5.588069690766758 1108 56-57 6.925426699944115 1108 58-59 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-14.021950577130413 2313 80-81 -13.391248526843139 2313 82-83 -12.879618043512266 2313 84-85 -12.346766780114294 2313 86-87 -11.772653294484417 2313 88-89 -11.21742622496403 2313 90-91 -10.657313309386844 2313 92-93 -10.050499861487038 2313 94-95 -9.620248866955366 2313 96-97 -8.847620178075672 2313 98-99 -7.969188535068612 2313 100-101 -6.865855588414037 2314 1 -20.96024096975724 2314 2 -19.764827342241336 2314 3 -20.210635707016817 2314 4 -22.138642904946916 2314 5 -22.12782442189889 2314 6 -22.92710666256284 2314 7 -23.016636507199966 2314 8 -23.856220484413797 2314 9 -25.065855836637308 2314 10-11 -25.36560413992468 2314 12-13 -25.339691025063257 2314 14-15 -25.57376763067752 2314 16-17 -24.679277514421756 2314 18-19 -24.011400456033076 2314 20-21 -23.490022002277613 2314 22-23 -23.39596915219133 2314 24-25 -23.123878082295807 2314 26-27 -22.809542409552552 2314 28-29 -22.746563764579346 2314 30-31 -22.674154680132663 2314 32-33 -22.383565111677893 2314 34-35 -19.550967188593976 2314 36-37 -17.85579089675201 2314 38-39 -16.948704846803466 2314 40-41 -18.332069820417498 2314 42-43 -18.26054951658317 2314 44-45 -16.238761917234278 2314 46-47 -17.077628465288832 2314 48-49 -16.573363177365383 2314 50-51 -14.496720866626504 2314 52-53 -17.472262461620463 2314 54-55 -19.107274438787897 2314 56-57 -19.324952854711757 2314 58-59 -18.94812219894862 2314 60-61 -18.64918832372624 2314 62-63 -18.162960646410237 2314 64-65 -17.66609545969739 2314 66-67 -17.24102152550628 2314 68-69 -16.786211441521154 2314 70-71 -16.200739561442052 2314 72-73 -15.744996307334265 2314 74-75 -15.192179355716643 2314 76-77 -14.629562945998138 2314 78-79 -14.133860228054438 2314 80-81 -13.620971319450943 2314 82-83 -13.131799768358263 2314 84-85 -12.602413597362753 2314 86-87 -12.02855678524417 2314 88-89 -11.470634643855199 2314 90-91 -10.910521728278015 2314 92-93 -10.280992674628722 2314 94-95 -9.620248866955366 2314 96-97 -8.893821410108528 2314 98-99 -8.087258350263683 2314 100-101 -7.006897682869889 2315 1 -20.96024096975724 2315 2 -19.764827342241336 2315 3 -20.210635707016817 2315 4 -16.269836029314966 2315 5 -22.12782442189889 2315 6 -22.92710666256284 2315 7 -23.016636507199966 2315 8 -23.856220484413797 2315 9 -19.60528960812366 2315 10-11 -17.63797522182559 2315 12-13 -13.698260286943945 2315 14-15 -13.545835375874688 2315 16-17 -19.95518752453298 2315 18-19 -24.011400456033076 2315 20-21 -23.490022002277613 2315 22-23 -23.39596915219133 2315 24-25 -23.123878082295807 2315 26-27 -22.809542409552552 2315 28-29 -22.746563764579346 2315 30-31 -22.674154680132663 2315 32-33 -22.383565111677893 2315 34-35 -19.550967188593976 2315 36-37 -17.85579089675201 2315 38-39 -16.948704846803466 2315 40-41 -18.332069820417498 2315 42-43 -18.26054951658317 2315 44-45 -16.238761917234278 2315 46-47 -17.077628465288832 2315 48-49 -16.573363177365383 2315 50-51 -14.496720866626504 2315 52-53 -17.472262461620463 2315 54-55 -19.107274438787897 2315 56-57 -19.324952854711757 2315 58-59 -18.94812219894862 2315 60-61 -18.64918832372624 2315 62-63 -18.162960646410237 2315 64-65 -17.66609545969739 2315 66-67 -17.24102152550628 2315 68-69 -16.786211441521154 2315 70-71 -16.200739561442052 2315 72-73 -15.744996307334265 2315 74-75 -15.192179355716643 2315 76-77 -14.629562945998138 2315 78-79 -14.133860228054438 2315 80-81 -13.620971319450943 2315 82-83 -13.131799768358263 2315 84-85 -12.602413597362753 2315 86-87 -12.02855678524417 2315 88-89 -11.470634643855199 2315 90-91 -10.910521728278015 2315 92-93 -10.280992674628722 2315 94-95 -9.620248866955366 2315 96-97 -8.893821410108528 2315 98-99 -8.087258350263683 2315 100-101 -7.006897682869889 2316 1 -20.96024096975724 2316 2 -19.764827342241336 2316 3 -20.210635707016817 2316 4 -22.138642904946916 2316 5 -22.12782442189889 2316 6 -22.92710666256284 2316 7 -23.016636507199966 2316 8 -23.856220484413797 2316 9 -25.065855836637308 2316 10-11 -25.36560413992468 2316 12-13 -25.339691025063257 2316 14-15 -25.57376763067752 2316 16-17 -24.679277514421756 2316 18-19 -24.011400456033076 2316 20-21 -23.490022002277613 2316 22-23 -23.39596915219133 2316 24-25 -23.123878082295807 2316 26-27 -22.809542409552552 2316 28-29 -22.746563764579346 2316 30-31 -22.674154680132663 2316 32-33 -22.383565111677893 2316 34-35 -19.550967188593976 2316 36-37 -17.85579089675201 2316 38-39 -16.948704846803466 2316 40-41 -18.332069820417498 2316 42-43 -18.26054951658317 2316 44-45 -16.238761917234278 2316 46-47 -17.077628465288832 2316 48-49 -16.573363177365383 2316 50-51 -14.496720866626504 2316 52-53 -17.472262461620463 2316 54-55 -19.107274438787897 2316 56-57 -19.324952854711757 2316 58-59 -18.94812219894862 2316 60-61 -18.64918832372624 2316 62-63 -18.162960646410237 2316 64-65 -17.66609545969739 2316 66-67 -17.24102152550628 2316 68-69 -16.786211441521154 2316 70-71 -16.200739561442052 2316 72-73 -15.744996307334265 2316 74-75 -15.192179355716643 2316 76-77 -14.629562945998138 2316 78-79 -14.133860228054438 2316 80-81 -13.620971319450943 2316 82-83 -13.131799768358263 2316 84-85 -12.602413597362753 2316 86-87 -12.02855678524417 2316 88-89 -11.470634643855199 2316 90-91 -10.910521728278015 2316 92-93 -10.280992674628722 2316 94-95 -9.620248866955366 2316 96-97 -8.893821410108528 2316 98-99 -8.087258350263683 2316 100-101 -7.006897682869889 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 591025.0 3 35533.0 4 81223.0 5 44710.0 6 38547.0 7 46504.0 8 59879.0 9 72634.0 10 76107.0 11 71940.0 12 65578.0 13 59937.0 14 58055.0 15 61874.0 16 67648.0 17 71738.0 18 74292.0 19 78068.0 20 81754.0 21 86964.0 22 92389.0 23 97676.0 24 103702.0 25 110234.0 26 118219.0 27 125359.0 28 133612.0 29 141707.0 30 148161.0 31 149692.0 32 144930.0 33 141985.0 34 133963.0 35 113716.0 36 82101.0 37 36729.0 38 5086.0 39 36.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.97010478506884 20.31669571213046 13.974146901518484 23.739052601282218 2 15.104702510819168 20.94343278600804 40.88373084062656 23.068133862546237 3 18.410944982100183 26.033474029243997 30.33109754582209 25.22448344283373 4 12.338942912230698 17.21442143795233 39.27973146673373 31.166904183083243 5 12.997256910475533 37.97806574695566 35.70913136660964 13.315545975959168 6 30.193581134751224 37.964563079836076 18.293146003417505 13.548709781995196 7 26.44996514931663 32.77769658157623 23.190112786300197 17.582225482806944 8 24.852844634574172 36.12220463255611 21.134260697719775 17.890690035149937 9 25.91452966687488 16.285729887981297 21.297540246883585 36.50220019826024 10-11 24.421512627686752 26.605539999372578 29.177396529617894 19.795550843322772 12-13 24.81667228826826 24.42289870695693 29.120125034878207 21.640303969896603 14-15 22.150678423456423 25.795596664282954 26.79675562126255 25.256969290998065 16-17 21.867480659942178 28.78538430938713 27.322736321897782 22.024398708772907 18-19 21.76175850443221 27.939964743854297 29.12149080233476 21.17678594937874 20-21 22.59570940858443 27.248318877767918 28.94090493527238 21.215066778375277 22-23 22.300380919408987 27.02503091454533 28.755872520504294 21.91871564554139 24-25 22.10052044681091 27.225252357861823 28.747861492780114 21.926365702547155 26-27 22.052016430758123 27.418831504506393 28.629139269502584 21.900012795232897 28-29 22.06375784126514 27.42451280497611 28.727172963992782 21.78455638976597 30-31 22.343454486862118 27.30656289874756 28.564485999517004 21.785496614873313 32-33 22.20382282787767 27.46457134796031 28.431205798108927 21.90040002605309 34-35 22.42983999449118 27.39897265810934 28.458918471261384 21.7122688761381 36-37 22.920150745439297 27.36616908992079 27.97869039053908 21.734989774100836 38-39 22.669682915626012 27.444376401552127 28.061600299631106 21.82434038319075 40-41 22.646122302547386 27.385378680855588 28.16551802618758 21.80298099040944 42-43 22.8491237510548 27.317892560221562 27.865727876123014 21.967255812600627 44-45 23.033895014465507 27.585588032369074 27.542497403422704 21.83801954974271 46-47 22.48580049601852 27.410291988576148 27.865976608820603 22.23793090658473 48-49 22.93229728831956 27.449032504100433 27.48652960295007 22.132140604629935 50-51 23.06209299987713 27.65402100440692 27.26453815823503 22.019347837480915 52-53 22.19295279452313 27.730667872474264 27.786145795188133 22.290233537814476 54-55 22.13186492370668 27.596985536413637 27.887266639957016 22.383882899922668 56-57 22.135489176386105 27.80873842257458 27.703556137241957 22.352216263797356 58-59 22.16828395512632 27.69514866598121 27.89204886700727 22.244518511885197 60-61 22.166969145315317 27.76659316432918 27.804014323410385 22.262423366945118 62-63 22.10399118565205 27.710339079972957 27.709392735237255 22.47627699913774 64-65 21.983936865882413 27.524037366500526 27.852651453866006 22.639374313751055 66-67 22.070682865304722 27.662454324440784 27.64968311533696 22.617179694917535 68-69 22.09818355481041 27.606377679214166 27.64652623041277 22.64891253556265 70-71 21.969086508188816 27.372656985500342 27.685575463140825 22.972681043170017 72-73 22.10342137306398 27.425819578616885 27.647261856636156 22.823497191682982 74-75 21.99128532732313 27.650136168666208 27.478126421599658 22.88045208241101 76-77 21.970477762990814 27.553147271805155 27.517516196809826 22.9588587683942 78-79 21.973015412276794 27.60430635252591 27.385595652942857 23.037082582254442 80-81 21.957015378878214 27.58081544809902 27.377427611932635 23.084741561090123 82-83 22.13881998673303 27.456648878965105 27.427322367497485 22.977208766804377 84-85 22.185792925355972 27.404960617834988 27.277905356395806 23.131341100413234 86-87 21.853257193700333 27.455222854910716 27.376811611675816 23.314708339713132 88-89 22.042863770230856 27.334877474213137 27.229642481303596 23.392616274252408 90-91 21.998068117250902 27.35174124739368 27.30616084226396 23.344029793091458 92-93 22.03931672070166 27.17803005784361 27.282297514989402 23.500355706465328 94-95 21.82624089415825 27.195697287279046 27.343147163697722 23.634914654864986 96-97 21.83613196814562 27.068941979522183 27.271717861205918 23.82320819112628 98-99 21.7670867994388 27.086114900638986 27.172424258578538 23.974374041343683 100-101 22.029498272808077 27.167769152179392 26.967893426299348 23.83483914871318 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 44671.0 1 48496.0 2 36263.5 3 21553.5 4 28059.0 5 35342.0 6 37242.5 7 35603.5 8 32491.5 9 29227.5 10 25984.5 11 22580.5 12 19196.5 13 16218.0 14 13566.5 15 11558.0 16 10314.0 17 9601.5 18 9408.5 19 9547.5 20 10126.5 21 11294.0 22 12902.5 23 15164.5 24 18192.5 25 22017.0 26 26838.5 27 32557.5 28 39233.0 29 47535.5 30 56235.0 31 64804.5 32 73637.0 33 81727.0 34 88992.5 35 95153.0 36 100907.5 37 106016.5 38 108686.5 39 109494.5 40 110528.0 41 110840.5 42 110911.0 43 112067.5 44 112730.0 45 113139.5 46 113575.0 47 113081.0 48 111888.5 49 109659.0 50 105905.0 51 101672.0 52 97010.0 53 92115.5 54 86649.5 55 79605.5 56 71801.5 57 63853.5 58 56097.5 59 48545.0 60 40979.0 61 33699.5 62 27020.0 63 21104.0 64 16192.5 65 12340.0 66 9216.5 67 6854.0 68 5113.0 69 3722.5 70 2714.0 71 1959.5 72 1410.5 73 1014.5 74 706.0 75 507.0 76 353.0 77 226.0 78 133.5 79 84.5 80 59.0 81 39.0 82 27.5 83 22.0 84 13.0 85 6.0 86 3.5 87 1.5 88 1.0 89 0.5 90 1.0 91 1.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.624685450058555 2 21.680595208552788 3 17.42159102661486 4 22.69179951864644 5 22.577172240918724 6 16.372150621053024 7 15.196309676729475 8 12.223156222262967 9 3.0806249657400806 10-11 6.6075132307421445 12-13 3.611447822176234 14-15 7.52505800896334 16-17 11.65270662140622 18-19 14.777643333377439 20-21 15.202466336169268 22-23 13.111686392729524 24-25 15.675435495895965 26-27 13.157577808159033 28-29 13.18886065886517 30-31 14.070599601923362 32-33 12.523077346814619 34-35 19.41517405929349 36-37 24.16749408029094 38-39 23.2534191737277 40-41 18.37237636523248 42-43 18.30401854342619 44-45 23.642274324002845 46-47 13.00058299244432 48-49 21.757566952996335 50-51 21.431642043179245 52-53 11.439073239134643 54-55 13.055817408602636 56-57 12.572398129563659 58-59 13.608661123692958 60-61 13.011816195632713 62-63 12.971649393366524 64-65 14.048038685423595 66-67 12.359912370213975 68-69 13.4060314200254 70-71 12.002002534491469 72-73 11.361088886230604 74-75 11.43710202799822 76-77 11.483506498381042 78-79 11.902280853302196 80-81 11.876884633113052 82-83 10.363305013600007 84-85 10.281000197931201 86-87 10.410776098227881 88-89 11.846398367729167 90-91 9.299998622852494 92-93 11.352110424547574 94-95 11.446188501250369 96-97 10.991716322735329 98-99 10.85005914983554 100-101 10.956639565663878 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3703307.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.80250708476595 #Duplication Level Percentage of deduplicated Percentage of total 1 98.77276716512795 95.61451493284233 2 0.861287895886906 1.6674965528723071 3 0.16981671326201436 0.49316050765973435 4 0.06561879514695512 0.2540825552842771 5 0.03330988346807985 0.1612240115205764 6 0.023932284725350358 0.13900230970082184 7 0.013965707328633569 0.0946340837836673 8 0.009006477394038221 0.06974796733961353 9 0.006845030656572202 0.059635451576544346 >10 0.039044181427457886 0.7021624198768714 >50 0.002604910521537562 0.1771030014703978 >100 0.0016615477685568247 0.2502122640849842 >500 5.576291634246767E-5 0.0395402770229401 >1k 5.5762913057383375E-5 0.09530150967720669 >5k 2.7881456528691688E-5 0.18218215528777826 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 6750 0.1822695228885966 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 2.700289227979209E-5 5 0.0 0.0 0.0 0.0 2.700289227979209E-5 6 0.0 0.0 0.0 0.0 8.100867683937626E-5 7 0.0 0.0 0.0 0.0 8.100867683937626E-5 8 0.0 0.0 0.0 0.0 8.100867683937626E-5 9 0.0 0.0 0.0 5.400578455958418E-5 8.100867683937626E-5 10-11 0.0 0.0 0.0 5.400578455958418E-5 8.100867683937626E-5 12-13 2.700289227979209E-5 0.0 0.0 8.100867683937627E-5 1.0801156911916836E-4 14-15 4.050433841968814E-5 0.0 0.0 1.215130152590644E-4 1.6201735367875255E-4 16-17 5.400578455958418E-5 0.0 0.0 1.3501446139896044E-4 1.8902024595854463E-4 18-19 5.400578455958418E-5 0.0 0.0 1.3501446139896044E-4 2.0252169209844067E-4 20-21 5.400578455958418E-5 0.0 0.0 2.1602313823833671E-4 2.1602313823833671E-4 22-23 5.400578455958418E-5 0.0 0.0 2.9703181507771296E-4 2.1602313823833671E-4 24-25 5.400578455958418E-5 0.0 0.0 4.3204627647667343E-4 2.1602313823833671E-4 26-27 5.400578455958418E-5 0.0 0.0 5.670607378756338E-4 2.1602313823833671E-4 28-29 5.400578455958418E-5 0.0 0.0 0.0011611243680310598 2.1602313823833671E-4 30-31 5.400578455958418E-5 0.0 0.0 0.0034563702118133874 2.1602313823833671E-4 32-33 5.400578455958418E-5 0.0 0.0 0.008708432760232948 2.1602313823833671E-4 34-35 5.400578455958418E-5 0.0 0.0 0.016579775859792343 2.1602313823833671E-4 36-37 5.400578455958418E-5 0.0 0.0 0.02921712944673504 2.1602313823833671E-4 38-39 5.400578455958418E-5 0.0 0.0 0.04891573936484337 2.1602313823833671E-4 40-41 5.400578455958418E-5 0.0 0.0 0.06974847075870297 2.1602313823833671E-4 42-43 5.400578455958418E-5 0.0 0.0 0.09091873830605997 2.1602313823833671E-4 44-45 5.400578455958418E-5 0.0 0.0 0.11858320144670696 2.1602313823833671E-4 46-47 5.400578455958418E-5 0.0 0.0 0.15185076473541081 2.2952458437823275E-4 48-49 5.400578455958418E-5 0.0 0.0 0.189101254635384 2.700289227979209E-4 50-51 5.400578455958418E-5 0.0 0.0 0.2310097434536213 2.9703181507771296E-4 52-53 5.400578455958418E-5 0.0 0.0 0.2881613649638013 3.10533261217609E-4 54-55 5.400578455958418E-5 0.0 0.0 0.3509025851758982 3.375361534974011E-4 56-57 5.400578455958418E-5 0.0 0.0 0.4270102370664922 3.510375996372971E-4 58-59 5.400578455958418E-5 0.0 0.0 0.5081539283672674 3.7804049191708926E-4 60-61 8.100867683937626E-5 0.0 0.0 0.597317478675141 4.0504338419688134E-4 62-63 8.100867683937626E-5 0.0 0.0 0.6876691562433253 4.3204627647667343E-4 64-65 8.100867683937626E-5 0.0 0.0 0.7822062821148774 4.3204627647667343E-4 66-67 9.451012297927232E-5 0.0 0.0 0.8905688888336829 4.4554772261656947E-4 68-69 1.0801156911916836E-4 0.0 0.0 1.0126894691690427 4.590491687564655E-4 70-71 1.0801156911916836E-4 0.0 0.0 1.1455977049701793 4.860520610362576E-4 72-73 1.0801156911916836E-4 0.0 0.0 1.2826913890746838 5.940636301554259E-4 74-75 1.0801156911916836E-4 0.0 0.0 1.4249561270507685 5.940636301554259E-4 76-77 1.0801156911916836E-4 0.0 0.0 1.5811003516586661 5.940636301554259E-4 78-79 1.0801156911916836E-4 0.0 0.0 1.758401342367781 5.940636301554259E-4 80-81 1.0801156911916836E-4 1.3501446139896045E-5 0.0 1.9522146017059887 6.21066522435218E-4 82-83 1.215130152590644E-4 2.700289227979209E-5 0.0 2.1518469843304917 6.750723069948022E-4 84-85 1.3501446139896044E-4 2.700289227979209E-5 0.0 2.351195836586057 6.750723069948022E-4 86-87 1.3501446139896044E-4 2.700289227979209E-5 0.0 2.57029730454429 6.750723069948022E-4 88-89 1.3501446139896044E-4 2.700289227979209E-5 0.0 2.8063430874080924 6.750723069948022E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2880 0.0 57.619442 1 GTATCAA 5590 0.0 44.483685 1 ATCAACG 6965 0.0 35.536373 3 TCAACGC 7110 0.0 34.939472 4 TATCAAC 7285 0.0 33.844406 2 CAACGCA 7365 0.0 32.766975 5 AACGCAG 8160 0.0 30.111473 6 ACGCAGA 9080 0.0 26.674482 7 CGCAGAG 9455 0.0 25.580238 8 GTACATG 7645 0.0 25.202957 1 TACATGG 7560 0.0 24.343254 2 ACATGGG 7960 0.0 22.988272 3 GCAGAGT 10160 0.0 22.443169 9 AGAGTAC 9050 0.0 19.653131 10-11 GTGGTAT 1620 0.0 19.303776 1 CAGAGTA 9705 0.0 19.16907 10-11 GAGTACT 5950 0.0 18.804865 12-13 GTGTAGC 1470 0.0 17.84233 1 AGTACTT 6425 0.0 17.59305 12-13 CATGGGG 5655 0.0 17.464752 4 >>END_MODULE