FastQCFastQC Report
Sat 18 Jun 2016
SRR1930954_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930954_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3703307
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57280.1546725669786491No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50590.13660763204346818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42050.055.223461
GTATCAA77050.041.2319871
ATCAACG96650.032.22473
TCAACGC96850.032.2054334
CAACGCA96950.032.061725
TATCAAC102650.030.434872
AACGCAG102700.030.4052646
ACGCAGA118800.026.0050627
CGCAGAG124200.024.721578
GTACATG103850.023.4123061
TACATGG104500.022.5356392
ACATGGG103950.022.3342463
GCAGAGT140900.021.3536249
AGAGTAC127600.019.52549210-11
CAGAGTA134500.018.7002310-11
TACGGGC5100.018.617584
CATGGGG70350.018.4230694
GTGGTAT21750.018.1217271
GAGTACT87350.018.03717612-13
TATACTG21850.017.8096665
Produced by FastQC (version 0.11.5)