##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930954_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3703307 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.227081362684757 31.0 31.0 33.0 30.0 34.0 2 31.351250652457384 31.0 31.0 34.0 30.0 34.0 3 31.43675558089027 31.0 31.0 34.0 28.0 34.0 4 35.305759419891466 37.0 35.0 37.0 33.0 37.0 5 35.09133188255794 35.0 35.0 37.0 32.0 37.0 6 35.126098646426016 37.0 35.0 37.0 32.0 37.0 7 35.00621417560035 36.0 35.0 37.0 32.0 37.0 8 34.98312238223836 36.0 35.0 37.0 32.0 37.0 9 36.48108352885678 38.0 35.0 39.0 32.0 39.0 10-11 36.41279645462825 38.0 35.0 39.0 32.0 39.0 12-13 36.3106983839039 38.0 35.0 39.0 32.0 39.0 14-15 37.319836162651384 39.0 36.0 41.0 32.0 41.0 16-17 37.28456728000136 39.0 36.0 40.5 32.0 41.0 18-19 37.27584359060699 39.0 36.0 40.0 31.5 41.0 20-21 37.24389606370738 39.0 36.0 40.0 31.0 41.0 22-23 37.117883151464355 39.0 36.0 40.0 31.0 41.0 24-25 36.999704048300615 39.0 36.0 40.0 31.0 41.0 26-27 36.72673991111188 39.0 35.5 40.0 30.0 41.0 28-29 36.537806884495396 38.0 35.0 40.0 30.0 41.0 30-31 36.30383154839715 38.0 35.0 40.0 30.0 41.0 32-33 36.07092768706456 38.0 34.5 40.0 29.5 41.0 34-35 36.27235360179429 38.0 35.0 40.0 30.0 41.0 36-37 36.325581973085136 38.0 35.0 40.0 30.0 41.0 38-39 36.292822739243604 38.0 35.0 40.0 30.0 41.0 40-41 36.197469450952894 38.0 35.0 40.0 30.0 41.0 42-43 36.06025074345713 38.0 35.0 40.0 29.5 41.0 44-45 35.873425022554166 38.0 35.0 40.0 29.0 41.0 46-47 35.664111157946124 38.0 34.0 40.0 28.0 41.0 48-49 35.46345847103683 38.0 34.0 40.0 27.0 41.0 50-51 35.2005407599208 38.0 34.0 40.0 27.0 41.0 52-53 34.99162248768465 38.0 33.5 40.0 26.0 41.0 54-55 34.82218325404834 38.0 33.0 40.0 26.0 41.0 56-57 34.57112129780221 37.5 33.0 40.0 26.0 41.0 58-59 34.327053225671 37.0 33.0 40.0 26.0 41.0 60-61 34.02352451471077 37.0 32.5 40.0 25.0 41.0 62-63 33.72506195138561 36.5 32.0 39.0 24.5 41.0 64-65 33.419792363960106 36.0 32.0 39.0 23.5 40.5 66-67 33.086417896220865 36.0 31.0 39.0 23.0 40.0 68-69 32.70909257590581 35.0 31.0 38.5 22.0 40.0 70-71 32.313215188478836 35.0 31.0 38.0 22.0 40.0 72-73 31.896727573490395 35.0 30.5 37.5 21.5 39.5 74-75 31.368447714434694 34.0 30.0 37.0 20.0 39.0 76-77 29.991427256773473 33.0 28.5 35.5 18.5 38.0 78-79 30.760358376985756 34.0 30.0 36.0 20.0 38.5 80-81 30.761185880619674 34.0 30.0 36.0 20.0 37.5 82-83 30.477663207506154 34.0 30.0 35.0 18.5 37.0 84-85 30.147491012762377 34.0 30.0 35.0 18.0 37.0 86-87 29.804389968209495 34.0 29.0 35.0 16.0 36.0 88-89 29.451199292956268 34.0 29.0 35.0 10.0 36.0 90-91 29.106681271631004 34.0 29.0 35.0 7.0 35.5 92-93 28.742670537441263 33.5 29.0 35.0 2.0 35.0 94-95 28.317201625466105 33.0 29.0 35.0 2.0 35.0 96-97 27.84943011745988 33.0 27.0 35.0 2.0 35.0 98-99 27.35032958380172 33.0 27.0 35.0 2.0 35.0 100-101 26.13144251880819 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4958915002382298 1101 2 0.5347528755005726 1101 3 0.49325133178403746 1101 4 0.32718856565406895 1101 5 0.45308890740947305 1101 6 0.42859524488708445 1101 7 0.4911657845924111 1101 8 0.45859219099860127 1101 9 0.6422347694796713 1101 10-11 0.5879613289718364 1101 12-13 0.5646062128170612 1101 14-15 0.7634699413320334 1101 16-17 0.6179439255578885 1101 18-19 0.6716376215814606 1101 20-21 0.6016194593271749 1101 22-23 0.697967452955055 1101 24-25 0.6249072244028184 1101 26-27 0.6608611227294432 1101 28-29 0.47066678902358206 1101 30-31 0.7529174245571113 1101 32-33 0.6840791782255096 1101 34-35 0.6587710715784851 1101 36-37 0.755027382133882 1101 38-39 0.8708377243368801 1101 40-41 0.842167523778258 1101 42-43 0.9147240091064432 1101 44-45 0.8580754278007561 1101 46-47 0.8555725532628244 1101 48-49 0.9228188805550914 1101 50-51 0.9334160416140023 1101 52-53 0.8233396556071924 1101 54-55 0.7535703741959523 1101 56-57 0.8189907127235401 1101 58-59 0.8023565748313288 1101 60-61 0.7823526673534431 1101 62-63 0.70031977415357 1101 64-65 0.8081585672758038 1101 66-67 0.9130937985913619 1101 68-69 0.7962039610725 1101 70-71 0.6724284088535626 1101 72-73 0.7407545862857674 1101 74-75 0.8018897620599539 1101 76-77 0.8221165899908378 1101 78-79 0.881102105438746 1101 80-81 0.7589767236668727 1101 82-83 0.8833076932884971 1101 84-85 0.9298186443788161 1101 86-87 0.9184754972189033 1101 88-89 0.7918850531453963 1101 90-91 0.8084074020710368 1101 92-93 0.8107812668413423 1101 94-95 0.8031858579003099 1101 96-97 0.6660860031443896 1101 98-99 0.9325844891269561 1101 100-101 0.9378766928452471 1102 1 0.47925639861758285 1102 2 0.5016048523898533 1102 3 0.5208601285762597 1102 4 0.2824377119803856 1102 5 0.3557173969810492 1102 6 0.40806543935798345 1102 7 0.4462354627027665 1102 8 0.46727296008229047 1102 9 0.5821453262416085 1102 10-11 0.5891815430432459 1102 12-13 0.5642656218609332 1102 14-15 0.6593701815138076 1102 16-17 0.6492622366165435 1102 18-19 0.6789282686961613 1102 20-21 0.7130874561798706 1102 22-23 0.7492607388652672 1102 24-25 0.7021521606454613 1102 26-27 0.7449839513375167 1102 28-29 0.752877531809375 1102 30-31 0.796509855560771 1102 32-33 0.8498580518265797 1102 34-35 0.7651076505968462 1102 36-37 0.8114743071589814 1102 38-39 0.881066526748647 1102 40-41 0.8566632180913984 1102 42-43 0.900813266010573 1102 44-45 0.9553637745330548 1102 46-47 0.85767150455127 1102 48-49 0.8577943566774096 1102 50-51 0.7884972659312623 1102 52-53 0.8642841368082159 1102 54-55 0.8619672941049927 1102 56-57 0.8403747825311356 1102 58-59 0.7594187118806701 1102 60-61 0.8073752688319829 1102 62-63 0.7238573887278932 1102 64-65 0.6739676130940211 1102 66-67 0.7718785573620153 1102 68-69 0.7232547453360567 1102 70-71 0.7129916216048358 1102 72-73 0.6164449040234956 1102 74-75 0.7650200066697401 1102 76-77 0.9153990525871052 1102 78-79 0.9773142334740719 1102 80-81 1.0445294067794535 1102 82-83 1.1034514096049826 1102 84-85 1.0979434133993102 1102 86-87 1.0679850713425267 1102 88-89 1.115986601001545 1102 90-91 1.068187939979584 1102 92-93 1.2475458513469526 1102 94-95 1.2220480633705257 1102 96-97 1.222709923252907 1102 98-99 1.204648965685866 1102 100-101 1.4104717264702522 1103 1 0.48709925492390127 1103 2 0.5781447007592959 1103 3 0.47804237445793873 1103 4 0.2798154186692017 1103 5 0.29000569809662124 1103 6 0.3838060087794375 1103 7 0.47470439046465174 1103 8 0.4327765328461055 1103 9 0.5486223564007702 1103 10-11 0.6131264857515433 1103 12-13 0.5997335402729718 1103 14-15 0.6682339347293791 1103 16-17 0.6642307081082564 1103 18-19 0.6901983528239057 1103 20-21 0.7938069866952802 1103 22-23 0.6310668880701584 1103 24-25 0.7118958507074922 1103 26-27 0.7456480687827352 1103 28-29 0.7557468895369865 1103 30-31 0.8022117490873413 1103 32-33 0.7959646905629256 1103 34-35 0.8348247173319834 1103 36-37 0.9529695033987693 1103 38-39 0.8540689389067211 1103 40-41 0.9058431579908586 1103 42-43 0.9713658687811133 1103 44-45 0.8734097084233241 1103 46-47 0.7949154625553945 1103 48-49 0.8109869644643126 1103 50-51 0.8605011889323961 1103 52-53 0.7650363122870871 1103 54-55 0.8326770228374869 1103 56-57 0.8067354839953182 1103 58-59 0.9446542839229153 1103 60-61 0.8083573418436458 1103 62-63 0.7914445035124302 1103 64-65 0.7734209254266986 1103 66-67 0.6241638156278029 1103 68-69 0.7538359290731123 1103 70-71 0.6523564154510026 1103 72-73 0.7835432094926418 1103 74-75 0.942766320169067 1103 76-77 1.0602954510521414 1103 78-79 1.0269471188454027 1103 80-81 1.021947268675202 1103 82-83 0.9608022927700048 1103 84-85 1.0822521238732001 1103 86-87 1.254564929776155 1103 88-89 1.1623178821022222 1103 90-91 1.2448626281067838 1103 92-93 1.265933042292101 1103 94-95 1.2921251270197658 1103 96-97 1.1415881757285042 1103 98-99 1.3147579721794678 1103 100-101 1.549410301129857 1104 1 0.4239766424706808 1104 2 0.476574427686721 1104 3 0.5094483872064082 1104 4 0.19694341756991207 1104 5 0.4139294801815936 1104 6 0.3668275078469918 1104 7 0.46943309513580544 1104 8 0.44136539579187684 1104 9 0.5681757589577145 1104 10-11 0.5495895046788064 1104 12-13 0.50095844643878 1104 14-15 0.6426383168791006 1104 16-17 0.706643641425984 1104 18-19 0.6822339371167274 1104 20-21 0.6522871821080187 1104 22-23 0.6218368666949985 1104 24-25 0.6984799689154997 1104 26-27 0.720067887248895 1104 28-29 0.6380440463466712 1104 30-31 0.6402058163045012 1104 32-33 0.7318480895901942 1104 34-35 0.7428137622333324 1104 36-37 0.6900422774991668 1104 38-39 0.7415758525158509 1104 40-41 0.7695732170609588 1104 42-43 0.7350223917205128 1104 44-45 0.7597216168385472 1104 46-47 0.7910409126721802 1104 48-49 0.7364223891842201 1104 50-51 0.716852344182179 1104 52-53 0.8551552849395776 1104 54-55 0.8535961603829207 1104 56-57 0.9080593617578856 1104 58-59 0.8265667732398114 1104 60-61 0.7360093251277675 1104 62-63 0.778394855219247 1104 64-65 0.7706736056605124 1104 66-67 0.7424344947444084 1104 68-69 0.7597669546495709 1104 70-71 0.7592137991855665 1104 72-73 0.744843020383982 1104 74-75 0.9903894926711452 1104 76-77 1.0364562999503093 1104 78-79 1.0065797262446132 1104 80-81 0.9410687575851036 1104 82-83 0.974500714282609 1104 84-85 1.0491583842229453 1104 86-87 1.1068302161053545 1104 88-89 1.119753858946094 1104 90-91 1.0440195778189896 1104 92-93 1.1277280143806259 1104 94-95 1.1869159835382384 1104 96-97 1.1926943738643736 1104 98-99 1.3901047345568074 1104 100-101 1.6249931909102848 1105 1 0.13380425269444984 1105 2 0.2613960154843369 1105 3 0.22642229786916346 1105 4 0.08744006920792202 1105 5 0.14265266969056256 1105 6 0.15067387702585933 1105 7 0.25763860464642363 1105 8 0.2048353773730085 1105 9 0.32998432628335905 1105 10-11 0.24271139938620934 1105 12-13 0.2147337870835102 1105 14-15 0.35341394119949143 1105 16-17 0.2958976011878889 1105 18-19 0.28285918660292 1105 20-21 0.2275783373363538 1105 22-23 0.4336526261743998 1105 24-25 0.3766675835989375 1105 26-27 0.3612355614855005 1105 28-29 0.4105784811222577 1105 30-31 0.35871708325453255 1105 32-33 0.40831580363764175 1105 34-35 0.4731629399616111 1105 36-37 0.4826563232674701 1105 38-39 0.3617584129065534 1105 40-41 0.34988825211134156 1105 42-43 0.2903805195360505 1105 44-45 0.41683000473466336 1105 46-47 0.4243466805388678 1105 48-49 0.4687787526494631 1105 50-51 0.4307071594628056 1105 52-53 0.3131171462048101 1105 54-55 0.38934584348699985 1105 56-57 0.4049418037320436 1105 58-59 0.4237385189306053 1105 60-61 0.4359305456442826 1105 62-63 0.2620027603854851 1105 64-65 0.36248118105714155 1105 66-67 0.39008901041434996 1105 68-69 0.26947963746463444 1105 70-71 0.2605954814383509 1105 72-73 0.23317468668837193 1105 74-75 0.3653248234447126 1105 76-77 0.32347931510971506 1105 78-79 0.41614775051719377 1105 80-81 0.34436035771097906 1105 82-83 0.4323823656545862 1105 84-85 0.4828779415605844 1105 86-87 0.3711611840489333 1105 88-89 0.40416283368452 1105 90-91 0.24454157043593128 1105 92-93 0.2718214113150559 1105 94-95 0.15068076321369617 1105 96-97 -0.05658079606697086 1105 98-99 0.25409235068402225 1105 100-101 0.356626796395787 1106 1 0.054966179840569396 1106 2 0.09935002962866335 1106 3 0.0675469276214713 1106 4 0.017839856969096957 1106 5 0.007894309837105595 1106 6 0.06013258634464336 1106 7 0.10498257870681726 1106 8 0.09285346642747783 1106 9 0.11279553630007655 1106 10-11 0.06931054589258423 1106 12-13 0.07562393495177844 1106 14-15 0.09749127481186548 1106 16-17 0.14662594677928809 1106 18-19 0.08802614119499452 1106 20-21 0.08989456202683499 1106 22-23 0.18492096318443885 1106 24-25 0.1011057919288092 1106 26-27 0.17997566060549275 1106 28-29 0.16248980398809465 1106 30-31 0.24841014479042656 1106 32-33 0.18814879134379225 1106 34-35 0.23964626408211132 1106 36-37 0.23090585221995497 1106 38-39 0.2553467336511801 1106 40-41 0.2253019706384265 1106 42-43 0.2310639964819785 1106 44-45 0.06649305875117761 1106 46-47 -0.047995332309312744 1106 48-49 0.11531459706056779 1106 50-51 0.09450235211349423 1106 52-53 -0.011292023217606584 1106 54-55 0.06563421727836527 1106 56-57 0.23186972072238632 1106 58-59 0.13559919778622032 1106 60-61 0.08711624592411482 1106 62-63 -0.016634892788431443 1106 64-65 -0.058345168091541666 1106 66-67 -0.024790975716477703 1106 68-69 0.06065358603121496 1106 70-71 -0.12304809945896267 1106 72-73 -0.11765985611319252 1106 74-75 0.11249484284418187 1106 76-77 0.17596529584613307 1106 78-79 0.20019870598736844 1106 80-81 -0.00160460862035805 1106 82-83 0.08731190659348087 1106 84-85 0.14220050060992762 1106 86-87 0.13901794022078562 1106 88-89 0.2384381323820044 1106 90-91 0.14890560150568533 1106 92-93 0.22893770711486994 1106 94-95 0.26670889808337606 1106 96-97 0.26649021070722156 1106 98-99 0.317528197390228 1106 100-101 0.474525020049704 1107 1 0.11616612922057001 1107 2 0.17930325848610096 1107 3 0.12129421864062451 1107 4 0.09429431678385924 1107 5 0.12618088671329275 1107 6 0.13406102647098095 1107 7 0.1522202067844205 1107 8 0.16683637030170217 1107 9 0.16366838011349927 1107 10-11 0.18902895107656548 1107 12-13 0.199342807770563 1107 14-15 0.20633793695031244 1107 16-17 0.2379272051153336 1107 18-19 0.23623713158344373 1107 20-21 0.3313880971863412 1107 22-23 0.262714573546063 1107 24-25 0.34217383304849847 1107 26-27 0.37189649036484695 1107 28-29 0.43320716242098456 1107 30-31 0.3969608152947188 1107 32-33 0.38864408581644483 1107 34-35 0.3437131736583723 1107 36-37 0.4116833544652252 1107 38-39 0.48000519231817407 1107 40-41 0.34831351708068325 1107 42-43 0.45007836993115546 1107 44-45 0.48089670242060834 1107 46-47 0.4553456961833362 1107 48-49 0.40842501595638936 1107 50-51 0.38332482034414994 1107 52-53 0.2989803823573851 1107 54-55 0.3589239579045014 1107 56-57 0.4525534750866669 1107 58-59 0.5337246615740838 1107 60-61 0.4440228365739429 1107 62-63 0.40671159612593755 1107 64-65 0.39966593204169243 1107 66-67 0.38161326507484006 1107 68-69 0.49383359082504086 1107 70-71 0.35551642514148085 1107 72-73 0.3983735880758985 1107 74-75 0.6917425331151641 1107 76-77 0.5777327753571058 1107 78-79 0.5693500624667074 1107 80-81 0.682561107005835 1107 82-83 0.6927461563352395 1107 84-85 0.7097126579017434 1107 86-87 0.7653655668081392 1107 88-89 0.5517249755112168 1107 90-91 0.6329132764885124 1107 92-93 0.6722066582963251 1107 94-95 0.7396318595269982 1107 96-97 0.7177231601381742 1107 98-99 0.6753912557346275 1107 100-101 1.1334943245878684 1108 1 0.302828433984466 1108 2 0.3174752321344734 1108 3 0.32071534499450394 1108 4 0.1916653488346256 1108 5 0.2672330216906289 1108 6 0.16810025083046298 1108 7 0.30361611806372224 1108 8 0.37288367237268716 1108 9 0.4069092226774771 1108 10-11 0.37733900045002855 1108 12-13 0.4283444311970541 1108 14-15 0.4939240870898729 1108 16-17 0.42923318286758416 1108 18-19 0.39717291331628957 1108 20-21 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-0.9090469714337424 2316 84-85 -0.8816788934427997 2316 86-87 -0.7150945614758335 2316 88-89 -0.8547498017436261 2316 90-91 -0.5520546295900495 2316 92-93 -0.8797959501229791 2316 94-95 -0.6629557401173223 2316 96-97 -0.5238152982254825 2316 98-99 -0.5302321395119769 2316 100-101 -0.7258100464863766 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 19.0 4 56.0 5 116.0 6 228.0 7 644.0 8 1494.0 9 2716.0 10 4608.0 11 7090.0 12 9842.0 13 12516.0 14 14746.0 15 18003.0 16 21553.0 17 24146.0 18 22221.0 19 19828.0 20 20140.0 21 21918.0 22 24686.0 23 28390.0 24 35156.0 25 44202.0 26 56723.0 27 72726.0 28 91294.0 29 113558.0 30 139363.0 31 167225.0 32 198944.0 33 240547.0 34 292241.0 35 362458.0 36 466946.0 37 589479.0 38 492654.0 39 84801.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.97620603788668 21.619150763513403 14.732594757225964 23.672048441373946 2 15.308485553528472 21.957405349120812 39.797898459381486 22.936210637969232 3 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21.25959311501855 22-23 21.968006424529865 27.225524976872016 28.912423115994194 21.89404548260392 24-25 21.837436222164914 27.411068821330254 28.95807371023294 21.79342124627189 26-27 21.878853046982126 27.571438832718826 28.773116931722996 21.776591188576052 28-29 21.846556231063367 27.572685421898203 28.872619035242714 21.708139311795723 30-31 22.14332654210577 27.440186991223342 28.754423873327518 21.66206259334337 32-33 22.000240919014207 27.543822547480897 28.63319782989227 21.822738703612625 34-35 22.032764261784614 27.503953073539993 28.75369144826301 21.709591216412377 36-37 22.109857943594633 27.37907518532545 28.735493443991917 21.775573427087995 38-39 21.983024519463477 27.491218938873825 28.722045914671646 21.803710626991055 40-41 22.02436172888696 27.474149906844747 28.75908346410893 21.74240490015936 42-43 22.13597825436778 27.426666484831948 28.507237314429002 21.93011794637127 44-45 22.03698215164586 27.623489317114963 28.417561154765625 21.92196737647355 46-47 22.209052748468274 27.492068610167735 28.23605836120257 22.062820280161425 48-49 22.16425647314454 27.54655541131632 28.199304717073105 22.089883398466036 50-51 22.080786502901177 27.675304855366008 28.057785962625477 22.18612267910735 52-53 22.202865026992008 27.725555225956416 27.998636216029 22.072943531022574 54-55 22.175923418955584 27.591627540299292 28.055688381221017 22.176760659524106 56-57 22.15031769659705 27.7529609193217 27.915536022537985 22.181185361543264 58-59 22.091993638437515 27.735091725309818 28.101514915600724 22.071399720651947 60-61 22.176592818177962 27.810425316178 28.018256744828935 21.994725120815104 62-63 22.072969447876755 27.73649619775948 27.99806958703569 22.192464767328072 64-65 21.95566105796196 27.640816564838882 28.0505288890775 22.352993488121655 66-67 22.030526022996696 27.721636556837176 27.876472434321126 22.371364985845005 68-69 22.059087280182414 27.68293584508764 27.931755976785254 22.326220897944687 70-71 22.007414527722855 27.414170956311366 28.04055300435944 22.537861511606334 72-73 22.088864923786424 27.506683439836475 27.95433576126363 22.450115875113475 74-75 21.970626322297313 27.697973758691703 27.75998774337308 22.5714121756379 76-77 21.890033317287415 27.641364891662963 27.8322368621493 22.636364928900328 78-79 21.908532805515247 27.742747283417007 27.70607474635391 22.642645164713837 80-81 21.96161209178199 27.69216062915114 27.683155087975496 22.663072191091373 82-83 22.05395069865933 27.538494648161766 27.836350051454012 22.57120460172489 84-85 22.146881152440237 27.511478254435833 27.536415425456223 22.805225167667707 86-87 21.788971316717735 27.573976448617408 27.773325300872976 22.863726933791877 88-89 21.930695642605485 27.42172695857279 27.702665391228503 22.944912007593224 90-91 21.869861100252994 27.405421965822107 27.847650048952673 22.877066884972226 92-93 21.974129304861794 27.352946339711202 27.74427470092326 22.92864965450374 94-95 21.76386258310961 27.345417879792894 27.76201231245487 23.128707224642625 96-97 21.714713362948306 27.260297350449207 27.81557132584471 23.20941796075778 98-99 21.62045976744569 27.26203903700125 27.69396380046267 23.42353739509039 100-101 21.644276318436468 27.216633673632785 27.682636087151298 23.456453920779456 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 253.0 1 196.0 2 168.5 3 222.0 4 220.5 5 223.0 6 364.0 7 661.5 8 1065.0 9 1368.5 10 1325.0 11 1204.5 12 1312.5 13 1503.5 14 1696.5 15 1931.5 16 2183.5 17 2481.0 18 2845.5 19 3349.5 20 3907.0 21 4674.5 22 5837.5 23 7583.5 24 10174.0 25 13357.0 26 18274.0 27 25003.5 28 32320.5 29 42338.5 30 53458.5 31 63875.0 32 75647.0 33 86488.5 34 95514.5 35 102090.5 36 111359.5 37 120723.5 38 122765.5 39 122473.0 40 123593.0 41 124395.5 42 124756.5 43 127248.5 44 130064.5 45 132701.0 46 134564.5 47 135061.0 48 134837.5 49 134116.0 50 131072.0 51 126334.5 52 122636.0 53 118198.5 54 113076.5 55 105782.5 56 95095.5 57 83528.0 58 73413.0 59 64673.5 60 54426.5 61 43380.0 62 33952.0 63 25932.0 64 19442.5 65 14105.5 66 9880.0 67 7356.5 68 5341.0 69 3659.0 70 2569.0 71 1792.5 72 1273.0 73 965.5 74 679.5 75 426.0 76 261.5 77 159.0 78 96.0 79 52.5 80 37.5 81 24.0 82 13.5 83 8.0 84 3.0 85 3.0 86 3.0 87 1.5 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018550986996217166 2 0.0032133441812952587 3 0.0035103759963729716 4 0.03388862981113907 5 0.0017551879981864858 6 2.7002892279792086E-5 7 0.0 8 0.0 9 2.7002892279792086E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.7002892279792086E-5 24-25 5.940636301554259E-4 26-27 0.0018631995673056541 28-29 6.750723069948022E-5 30-31 0.006629210054688957 32-33 0.02208836588486993 34-35 0.013285423001657708 36-37 6.615708608549062E-4 38-39 6.615708608549062E-4 40-41 0.0021737328285232633 42-43 0.008384398052875445 44-45 0.00822238069919669 46-47 0.008073864791657833 48-49 0.0095590238670464 50-51 0.19026237900341506 52-53 0.049712324687097235 54-55 0.01802443059676122 56-57 0.010882165588756211 58-59 0.020454690901942508 60-61 0.05373575563678626 62-63 0.09668385580779558 64-65 0.10153087497201825 66-67 0.1054732972448679 68-69 0.10389362804650007 70-71 0.09203935833567134 72-73 0.06900589122100868 74-75 0.24245896978025314 76-77 0.2910776773300188 78-79 0.007115262115725215 80-81 8.505911068134508E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.215130152590644E-4 90-91 0.006494195593289997 92-93 0.017875914689222363 94-95 0.0017551879981864858 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3703307.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.2568194149851 #Duplication Level Percentage of deduplicated Percentage of total 1 77.20505385574285 44.97720880398211 2 13.034841038955685 15.187367610189565 3 4.189360906519587 7.321765253859297 4 1.8232665294427246 4.248708358045258 5 1.0180951279056918 2.9655492006839026 6 0.6021204618274792 2.104657380645054 7 0.4018224917578475 1.6386230237451382 8 0.2852484296954569 1.3294133005741045 9 0.21135696922302377 1.1081686309311833 >10 1.1196241591705067 11.76744800199657 >50 0.07467702039806415 2.974875016306157 >100 0.033234252599968574 3.4686024356328358 >500 9.740675773441971E-4 0.3929530833512852 >1k 2.319208453998379E-4 0.22317421582354102 >5k 9.276833815993448E-5 0.29148568423399024 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5728 0.1546725669786491 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5059 0.13660763204346818 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.400578455958418E-5 2 0.0 0.0 0.0 0.0 5.400578455958418E-5 3 0.0 0.0 0.0 0.0 5.400578455958418E-5 4 0.0 0.0 0.0 0.0 5.400578455958418E-5 5 0.0 0.0 0.0 2.700289227979209E-5 5.400578455958418E-5 6 0.0 0.0 0.0 2.700289227979209E-5 5.400578455958418E-5 7 0.0 0.0 0.0 2.700289227979209E-5 5.400578455958418E-5 8 0.0 2.700289227979209E-5 0.0 2.700289227979209E-5 8.100867683937626E-5 9 0.0 2.700289227979209E-5 0.0 5.400578455958418E-5 8.100867683937626E-5 10-11 0.0 5.400578455958418E-5 0.0 5.400578455958418E-5 8.100867683937626E-5 12-13 0.0 8.100867683937627E-5 0.0 6.750723069948022E-5 2.2952458437823275E-4 14-15 0.0 1.0801156911916836E-4 0.0 8.100867683937626E-5 3.645390457771932E-4 16-17 0.0 1.0801156911916836E-4 0.0 8.100867683937626E-5 4.185448303367774E-4 18-19 0.0 1.0801156911916836E-4 0.0 1.0801156911916836E-4 5.130549533160497E-4 20-21 0.0 1.0801156911916836E-4 0.0 1.6201735367875255E-4 5.670607378756338E-4 22-23 0.0 1.0801156911916836E-4 0.0 2.430260305181288E-4 5.670607378756338E-4 24-25 0.0 1.0801156911916836E-4 0.0 4.590491687564655E-4 5.670607378756338E-4 26-27 0.0 1.0801156911916836E-4 0.0 7.560809838341785E-4 5.670607378756338E-4 28-29 0.0 1.0801156911916836E-4 0.0 0.002011715474844511 5.670607378756338E-4 30-31 0.0 1.0801156911916836E-4 0.0 0.00652119848556979 5.670607378756338E-4 32-33 0.0 1.0801156911916836E-4 0.0 0.01794342191992184 5.940636301554259E-4 34-35 0.0 1.0801156911916836E-4 0.0 0.037020965315594956 5.940636301554259E-4 36-37 0.0 1.0801156911916836E-4 0.0 0.06640011211600874 6.21066522435218E-4 38-39 0.0 1.0801156911916836E-4 0.0 0.11119791040818382 6.21066522435218E-4 40-41 0.0 1.0801156911916836E-4 0.0 0.157669888021706 6.21066522435218E-4 42-43 0.0 1.3501446139896044E-4 0.0 0.2058160449565753 6.34567968575114E-4 44-45 0.0 1.3501446139896044E-4 0.0 0.26634302800172927 6.615708608549061E-4 46-47 0.0 1.3501446139896044E-4 0.0 0.337293127466883 6.885737531346982E-4 48-49 0.0 1.3501446139896044E-4 0.0 0.41564201941669976 7.020751992745943E-4 50-51 0.0 1.3501446139896044E-4 0.0 0.4953815603189258 7.020751992745943E-4 52-53 0.0 1.3501446139896044E-4 0.0 0.5779429034643901 7.695824299740746E-4 54-55 0.0 1.3501446139896044E-4 0.0 0.6681325636788957 8.370896606735548E-4 56-57 0.0 1.3501446139896044E-4 0.0 0.7776292918734526 8.775939990932429E-4 58-59 0.0 1.3501446139896044E-4 0.0 0.8964015135661181 8.910954452331389E-4 60-61 0.0 1.6201735367875252E-4 0.0 1.0250162894947676 8.910954452331389E-4 62-63 0.0 1.6201735367875252E-4 0.0 1.1564528676666557 9.451012297927231E-4 64-65 0.0 1.6201735367875252E-4 0.0 1.2940596067244763 9.721041220725152E-4 66-67 0.0 1.6201735367875252E-4 0.0 1.4530526364678922 9.856055682124112E-4 68-69 0.0 1.6201735367875252E-4 0.0 1.633728988711981 9.991070143523073E-4 70-71 0.0 1.6201735367875252E-4 0.0 1.8351300607808103 0.0010396113527719954 72-73 0.0 1.6201735367875252E-4 0.0 2.042174737336116 0.0011746258141709558 74-75 0.0 1.6201735367875252E-4 0.0 2.2609926749254114 0.001255634491010332 76-77 0.0 1.6201735367875252E-4 0.0 2.500319309201209 0.001269135937150228 78-79 0.0 1.6201735367875252E-4 0.0 2.774317657164259 0.0012826373832901241 80-81 0.0 1.6201735367875252E-4 0.0 3.075197384391842 0.0013231417217098123 82-83 0.0 1.755187998186486E-4 0.0 3.3899971025896587 0.0014041503985491885 84-85 0.0 1.8902024595854463E-4 0.0 3.7097788544130963 0.0014041503985491885 86-87 0.0 1.8902024595854463E-4 0.0 4.066716586013528 0.0014311532908289808 88-89 0.0 1.8902024595854463E-4 0.0 4.458906593485228 0.001498660521528461 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4205 0.0 55.22346 1 GTATCAA 7705 0.0 41.231987 1 ATCAACG 9665 0.0 32.2247 3 TCAACGC 9685 0.0 32.205433 4 CAACGCA 9695 0.0 32.06172 5 TATCAAC 10265 0.0 30.43487 2 AACGCAG 10270 0.0 30.405264 6 ACGCAGA 11880 0.0 26.005062 7 CGCAGAG 12420 0.0 24.72157 8 GTACATG 10385 0.0 23.412306 1 TACATGG 10450 0.0 22.535639 2 ACATGGG 10395 0.0 22.334246 3 GCAGAGT 14090 0.0 21.353624 9 AGAGTAC 12760 0.0 19.525492 10-11 CAGAGTA 13450 0.0 18.70023 10-11 TACGGGC 510 0.0 18.61758 4 CATGGGG 7035 0.0 18.423069 4 GTGGTAT 2175 0.0 18.121727 1 GAGTACT 8735 0.0 18.037176 12-13 TATACTG 2185 0.0 17.809666 5 >>END_MODULE