FastQCFastQC Report
Sat 18 Jun 2016
SRR1930953_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930953_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793171
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51180.18323260552254053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22250.049.1366271
GTATCAA42750.044.3439671
ATCAACG48050.037.249533
TCAACGC47600.037.170754
TATCAAC50600.036.4665262
CAACGCA49000.035.931825
AACGCAG53250.032.415416
ACGCAGA61900.027.6087237
CGCAGAG64250.026.2447228
GTACATG57000.025.6913471
TACATGG59100.023.1617982
GCAGAGT66950.023.0492129
ACATGGG62600.021.623973
GAGTACA44650.019.7683351
CATGGGG44800.019.1316174
TATACCG1558.6819264E-418.672485
CAGAGTA66950.018.38769510-11
AGAGTAC64600.018.06084810-11
GAGTACT42950.017.78479612-13
AGTACAT46050.017.312692
Produced by FastQC (version 0.11.5)