FastQCFastQC Report
Sat 18 Jun 2016
SRR1930953_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930953_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793171
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35440.1268808819796568No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31660.11334787594458055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30950.050.0241851
GTATCAA59050.041.5808871
TCAACGC70300.033.8331264
CAACGCA70600.033.7427025
ATCAACG71500.033.2682763
AACGCAG74050.032.3623056
TATCAAC74300.032.079612
ACGCAGA86050.027.6286727
CGCAGAG88800.026.5058568
TATACCG1551.4235738E-624.492845
GTACATG82100.024.4692291
TACATGG85400.022.7394332
ACATGGG83600.022.6602783
GCAGAGT103700.021.9652219
GTGGTAT15900.021.5059411
TGGTATC17550.020.0202162
CATGGGG59700.019.4828054
AGAGTAC95600.018.66392710-11
CAGAGTA101500.018.04655810-11
GAGTACT62100.017.95764512-13
Produced by FastQC (version 0.11.5)