##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930953_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2793171 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.26272505335334 31.0 31.0 33.0 30.0 34.0 2 31.388037467093852 31.0 31.0 34.0 30.0 34.0 3 31.469892462724264 31.0 31.0 34.0 30.0 34.0 4 35.33854103454461 37.0 35.0 37.0 33.0 37.0 5 35.129917215952766 37.0 35.0 37.0 32.0 37.0 6 35.16656087292901 37.0 35.0 37.0 32.0 37.0 7 35.04647513524951 37.0 35.0 37.0 32.0 37.0 8 35.0243973605626 37.0 35.0 37.0 32.0 37.0 9 36.53173042395184 38.0 35.0 39.0 32.0 39.0 10-11 36.455886517510024 38.0 35.0 39.0 32.0 39.0 12-13 36.35966415948039 38.0 35.0 39.0 32.0 39.0 14-15 37.38580917530649 39.0 36.0 41.0 32.0 41.0 16-17 37.35168058095978 39.0 36.0 41.0 32.0 41.0 18-19 37.335482861593505 39.0 36.0 41.0 32.0 41.0 20-21 37.301099896855575 39.0 36.0 40.0 31.5 41.0 22-23 37.17949187500514 39.0 36.0 40.0 31.0 41.0 24-25 37.069175857833265 39.0 36.0 40.0 31.0 41.0 26-27 36.80360672511637 39.0 36.0 40.0 30.5 41.0 28-29 36.620723543241716 38.5 35.0 40.0 30.0 41.0 30-31 36.38695017240262 38.0 35.0 40.0 30.0 41.0 32-33 36.16402039116116 38.0 35.0 40.0 30.0 41.0 34-35 36.36692830478334 38.0 35.0 40.0 30.0 41.0 36-37 36.42906556741424 38.0 35.0 40.0 30.0 41.0 38-39 36.39539863474166 38.0 35.0 40.0 30.0 41.0 40-41 36.3065184695101 38.0 35.0 40.0 30.0 41.0 42-43 36.174512588022715 38.0 35.0 40.0 30.0 41.0 44-45 36.007582421556 38.0 35.0 40.0 29.0 41.0 46-47 35.8054424523239 38.0 34.5 40.0 28.5 41.0 48-49 35.62585158588572 38.0 34.0 40.0 28.0 41.0 50-51 35.36077902140613 38.0 34.0 40.0 27.5 41.0 52-53 35.15470194986272 38.0 34.0 40.0 27.0 41.0 54-55 34.99342199242366 38.0 33.5 40.0 26.5 41.0 56-57 34.753538361954924 38.0 33.0 40.0 26.0 41.0 58-59 34.51623853319399 37.0 33.0 40.0 26.0 41.0 60-61 34.212876691043974 37.0 33.0 40.0 26.0 41.0 62-63 33.91175960941883 36.5 32.0 39.5 25.0 41.0 64-65 33.613905843931505 36.0 32.0 39.0 24.5 41.0 66-67 33.288978727045354 36.0 32.0 39.0 23.5 40.0 68-69 32.93141665870081 35.0 31.0 39.0 23.0 40.0 70-71 32.5340353311702 35.0 31.0 38.0 22.5 40.0 72-73 32.12003454138683 35.0 31.0 37.5 22.0 40.0 74-75 31.60126698293803 34.5 30.0 37.0 20.5 39.0 76-77 30.22844734532902 33.5 28.5 35.5 19.5 38.5 78-79 30.99944346407721 34.0 30.0 36.0 20.0 39.0 80-81 31.002536364583477 34.0 30.0 36.0 20.0 38.0 82-83 30.715792910638122 34.0 30.0 35.0 20.0 37.0 84-85 30.388417143096504 34.0 30.0 35.0 18.5 37.0 86-87 30.038907392350843 34.0 29.5 35.0 18.0 36.0 88-89 29.688473960240888 34.0 29.0 35.0 15.0 36.0 90-91 29.33889726049712 34.0 29.0 35.0 9.0 36.0 92-93 28.97472979634974 34.0 29.0 35.0 4.5 35.0 94-95 28.550756111960204 33.0 29.0 35.0 2.0 35.0 96-97 28.089367246044013 33.0 28.5 35.0 2.0 35.0 98-99 27.598810455929836 33.0 27.0 35.0 2.0 35.0 100-101 26.389138366394324 32.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5630983529351532 1101 2 0.6089152835132587 1101 3 0.57034114210078 1101 4 0.31102963830229413 1101 5 0.6353170577068639 1101 6 0.5309064295717079 1101 7 0.556726320878262 1101 8 0.45519248086648645 1101 9 0.6113369575676515 1101 10-11 0.6303721679000702 1101 12-13 0.6872582272043744 1101 14-15 0.7873118749403929 1101 16-17 0.7242150045451439 1101 18-19 0.7571529427037405 1101 20-21 0.7619778241563893 1101 22-23 0.8503065133147203 1101 24-25 0.8230413186977543 1101 26-27 0.827538746125164 1101 28-29 0.7997921448137291 1101 30-31 0.792396546120564 1101 32-33 0.8210055465808068 1101 34-35 0.8069587760834196 1101 36-37 0.8282796444092497 1101 38-39 0.932988842855643 1101 40-41 0.8941187174918994 1101 42-43 0.8747935224639534 1101 44-45 0.8984833620637644 1101 46-47 0.8582947849209219 1101 48-49 0.9509248264878281 1101 50-51 0.9549661689014002 1101 52-53 0.811334126666587 1101 54-55 0.8136669169117141 1101 56-57 0.903320314277444 1101 58-59 0.7448991234607618 1101 60-61 0.6966426593780071 1101 62-63 0.7458273727696749 1101 64-65 0.7767805609554586 1101 66-67 0.8353056849506473 1101 68-69 0.7753581641104503 1101 70-71 0.6327395435971326 1101 72-73 0.7502097057040444 1101 74-75 0.7125267233731982 1101 76-77 0.8240418563210135 1101 78-79 0.8122289709434405 1101 80-81 0.6696837742393846 1101 82-83 0.6633664471025149 1101 84-85 0.8461571063905389 1101 86-87 0.8220280348892075 1101 88-89 0.8440542362384242 1101 90-91 0.6595958716170642 1101 92-93 0.8754957263854024 1101 94-95 1.0042932391691366 1101 96-97 1.1232686551727085 1101 98-99 1.1432020208358473 1101 100-101 1.424055287472516 1102 1 0.3921721672708536 1102 2 0.4630973749901841 1102 3 0.4424630791635309 1102 4 0.1548411187847023 1102 5 0.3455614850031168 1102 6 0.2951359427054925 1102 7 0.5285523696797227 1102 8 0.5320416596224078 1102 9 0.6596600861369595 1102 10-11 0.6397386488806944 1102 12-13 0.6154638807166322 1102 14-15 0.6778185847049301 1102 16-17 0.6667549519179659 1102 18-19 0.7723868092644821 1102 20-21 0.6434600983405119 1102 22-23 0.5804397300562201 1102 24-25 0.717387158545435 1102 26-27 0.6747820746759743 1102 28-29 0.7942653524489103 1102 30-31 0.744023819851158 1102 32-33 0.8976061783035831 1102 34-35 0.8221640457720341 1102 36-37 0.7412968959191844 1102 38-39 0.8468120569921425 1102 40-41 0.8047560613429212 1102 42-43 0.8330674465401486 1102 44-45 0.8786041793802397 1102 46-47 0.8684296951334431 1102 48-49 0.7112350042486639 1102 50-51 0.7085408896238974 1102 52-53 0.744163090114867 1102 54-55 0.7799716469684128 1102 56-57 0.8497733414574284 1102 58-59 0.810333681976438 1102 60-61 0.8573917097607833 1102 62-63 0.6771242811350149 1102 64-65 0.7870492955930217 1102 66-67 0.7370519708201684 1102 68-69 0.7907657343280547 1102 70-71 0.6470365274760823 1102 72-73 0.7229921858303427 1102 74-75 0.9948663228471979 1102 76-77 1.055940706503339 1102 78-79 1.0901773866543891 1102 80-81 1.0728126518523702 1102 82-83 1.1729854965514228 1102 84-85 1.1274998367752076 1102 86-87 1.124434707433128 1102 88-89 1.1412697945454369 1102 90-91 1.046155654495312 1102 92-93 1.248903740748176 1102 94-95 1.242252392111915 1102 96-97 1.208102281698455 1102 98-99 1.2544342466510479 1102 100-101 1.4995147252669483 1103 1 0.29783660506791065 1103 2 0.33124767204830974 1103 3 0.29035664984459686 1103 4 0.22545928677502047 1103 5 0.2827558603446434 1103 6 0.2745363332217252 1103 7 0.4139548674128761 1103 8 0.38742984966772553 1103 9 0.3601734900820972 1103 10-11 0.4187724506545507 1103 12-13 0.448366659656088 1103 14-15 0.5568654317888289 1103 16-17 0.5252277587695033 1103 18-19 0.6334846519739799 1103 20-21 0.6183257460678959 1103 22-23 0.5518844781763548 1103 24-25 0.5966321811939181 1103 26-27 0.6493274134513527 1103 28-29 0.6371986547515647 1103 30-31 0.7779121741830792 1103 32-33 0.7634859702087056 1103 34-35 0.6302594926260383 1103 36-37 0.7564109303432787 1103 38-39 0.582688012954911 1103 40-41 0.6549963457484296 1103 42-43 0.6068785207215939 1103 44-45 0.6160234975764354 1103 46-47 0.6184107261877188 1103 48-49 0.6466847357659802 1103 50-51 0.6268592781642539 1103 52-53 0.4843640211684104 1103 54-55 0.6177244303561409 1103 56-57 0.6423265105248745 1103 58-59 0.6297357297795187 1103 60-61 0.3995263417976318 1103 62-63 0.5109840489329329 1103 64-65 0.46114317346007283 1103 66-67 0.28244870687472456 1103 68-69 0.35596854563674185 1103 70-71 0.278124144703618 1103 72-73 0.1885916699637633 1103 74-75 0.42456197443270227 1103 76-77 0.5829314086139057 1103 78-79 0.7649717843592967 1103 80-81 0.6013913098582542 1103 82-83 0.42271909933992546 1103 84-85 0.6524981340072706 1103 86-87 0.5934420569566967 1103 88-89 0.5729048821697624 1103 90-91 0.6913222229154137 1103 92-93 0.7431278631682083 1103 94-95 0.7467309491053875 1103 96-97 0.5329496371500966 1103 98-99 0.8451748608499834 1103 100-101 0.9215914609753995 1104 1 0.4158197672992898 1104 2 0.50022603649602 1104 3 0.4604306898238697 1104 4 0.30085717878962015 1104 5 0.36459537684997656 1104 6 0.3855643139974063 1104 7 0.40622607527396326 1104 8 0.36672348175787306 1104 9 0.5368878521873555 1104 10-11 0.5012011274833199 1104 12-13 0.6072100254368138 1104 14-15 0.6054861972638221 1104 16-17 0.5481198006671946 1104 18-19 0.7438157717574327 1104 20-21 0.6947997713741074 1104 22-23 0.5473950579520377 1104 24-25 0.6305396597143726 1104 26-27 0.7978400852292893 1104 28-29 0.7203620215454336 1104 30-31 0.723452209245572 1104 32-33 0.8301721718370203 1104 34-35 0.6597244959678719 1104 36-37 0.6818364871824798 1104 38-39 0.6864402758698702 1104 40-41 0.6472534155085441 1104 42-43 0.560285344522427 1104 44-45 0.7496955303165649 1104 46-47 0.6988391073718816 1104 48-49 0.7350848224068827 1104 50-51 0.7588480580799839 1104 52-53 0.8227771541650313 1104 54-55 0.7600552584252611 1104 56-57 0.8638258457611911 1104 58-59 0.6650827835848716 1104 60-61 0.6206736437785096 1104 62-63 0.654674536534749 1104 64-65 0.8842336509741671 1104 66-67 0.7491675080161642 1104 68-69 0.672681495023653 1104 70-71 0.7225311119597748 1104 72-73 0.5153100824438965 1104 74-75 0.8080448077212345 1104 76-77 0.9114347699883538 1104 78-79 0.9492224093288648 1104 80-81 0.8370441942601197 1104 82-83 0.9185848238449985 1104 84-85 0.8579342392890688 1104 86-87 0.8528589601863636 1104 88-89 0.9261382931552973 1104 90-91 0.9867868620510301 1104 92-93 0.924444641131025 1104 94-95 0.9597520090895806 1104 96-97 0.9340993649889242 1104 98-99 1.0771445788936695 1104 100-101 1.4397225110181608 1105 1 0.19596529327617773 1105 2 0.3686907909819013 1105 3 0.3830476949073294 1105 4 0.144206595690747 1105 5 0.20273008039720963 1105 6 0.24296548286142894 1105 7 0.354284113983347 1105 8 0.4007344940775326 1105 9 0.4726875806619901 1105 10-11 0.42758423013426494 1105 12-13 0.38556882953730565 1105 14-15 0.5350708215493185 1105 16-17 0.45439575203020866 1105 18-19 0.4857784497007316 1105 20-21 0.5648781573610933 1105 22-23 0.5557174317851903 1105 24-25 0.45181701899471705 1105 26-27 0.4795899107841066 1105 28-29 0.5378341563794677 1105 30-31 0.44330527426750876 1105 32-33 0.5864646085137935 1105 34-35 0.6112905140676901 1105 36-37 0.6197520476929057 1105 38-39 0.5413804616022375 1105 40-41 0.5273119194050864 1105 42-43 0.5018362906450378 1105 44-45 0.5757810519190443 1105 46-47 0.49459035103218696 1105 48-49 0.5053393227024472 1105 50-51 0.5107458742799054 1105 52-53 0.38724464818273674 1105 54-55 0.5457151814677843 1105 56-57 0.6042499486122068 1105 58-59 0.5794747443010522 1105 60-61 0.483284757069363 1105 62-63 0.3932936546864383 1105 64-65 0.4298145138768348 1105 66-67 0.4740095210496449 1105 68-69 0.5304058128882474 1105 70-71 0.41430251257414596 1105 72-73 0.29810171263007845 1105 74-75 0.46869684577640314 1105 76-77 0.488520411464318 1105 78-79 0.6099648944210898 1105 80-81 0.5366836200206038 1105 82-83 0.6596377747595135 1105 84-85 0.5646750309786519 1105 86-87 0.6077917107279092 1105 88-89 0.6613324546012542 1105 90-91 0.5443787554819828 1105 92-93 0.590547183467713 1105 94-95 0.5389315346983956 1105 96-97 0.4749630545300363 1105 98-99 0.6041798973506971 1105 100-101 1.0754489931085658 1106 1 0.19821368564770836 1106 2 0.21218073067180399 1106 3 0.14648988310773703 1106 4 0.16875473175671374 1106 5 0.2107973800990237 1106 6 0.1446850906342334 1106 7 0.19792365295551662 1106 8 0.1762303249506587 1106 9 0.1848525475385827 1106 10-11 0.19405679719135804 1106 12-13 0.1390377501521769 1106 14-15 0.29228154991204036 1106 16-17 0.25041917113738066 1106 18-19 0.29255320301226817 1106 20-21 0.22766505130411474 1106 22-23 0.28620412238917936 1106 24-25 0.27767153374640685 1106 26-27 0.3462740754477025 1106 28-29 0.3396406133551295 1106 30-31 0.4577084740812438 1106 32-33 0.3621908523518158 1106 34-35 0.37342437975824794 1106 36-37 0.3426153500331637 1106 38-39 0.3116827380672973 1106 40-41 0.2829593728558635 1106 42-43 0.3599344982244119 1106 44-45 0.38718876505613764 1106 46-47 0.17255502111583354 1106 48-49 0.3323662533654925 1106 50-51 0.04708614960701851 1106 52-53 0.048179700615513354 1106 54-55 0.25606025642958485 1106 56-57 0.4193512497715588 1106 58-59 0.5286241977428148 1106 60-61 0.1601254994512331 1106 62-63 0.15959385501844991 1106 64-65 0.23872274840984886 1106 66-67 0.12440534163446415 1106 68-69 0.2859599413022096 1106 70-71 0.32155374980209217 1106 72-73 0.2332648145094396 1106 74-75 0.43710143440415195 1106 76-77 0.47321456845786614 1106 78-79 0.5822307661746606 1106 80-81 0.5556640423824426 1106 82-83 0.5760531762043009 1106 84-85 0.6225635632969286 1106 86-87 0.7009980776218114 1106 88-89 0.5856424668419749 1106 90-91 0.5766130513698187 1106 92-93 0.6889682613085313 1106 94-95 0.6697911173539879 1106 96-97 0.6032913700764837 1106 98-99 0.6401287767092398 1106 100-101 0.9124452177940157 1107 1 0.08460671574807677 1107 2 0.17049595659588945 1107 3 0.11277735677067469 1107 4 0.07235392089175008 1107 5 0.15050469497190733 1107 6 0.17621822220548466 1107 7 0.23268315620097013 1107 8 0.1227278098640383 1107 9 0.030490895518319405 1107 10-11 0.1484510642337895 1107 12-13 0.03897056841103819 1107 14-15 0.026750163591437115 1107 16-17 0.17474787417847182 1107 18-19 0.1432519755250894 1107 20-21 0.14075479986485107 1107 22-23 0.2181272029821102 1107 24-25 0.30256119782035285 1107 26-27 0.19987827393786262 1107 28-29 0.21737298888240986 1107 30-31 0.17459479338057093 1107 32-33 0.21971037148841788 1107 34-35 0.06853355767752589 1107 36-37 0.1685360304727368 1107 38-39 0.24517162163601114 1107 40-41 0.1683749281396345 1107 42-43 0.2637741797768811 1107 44-45 0.19618227067659433 1107 46-47 0.2059132060551505 1107 48-49 0.2643929907442981 1107 50-51 0.2930461372202515 1107 52-53 0.11437247361235592 1107 54-55 0.21593553866663484 1107 56-57 0.22875406958311117 1107 58-59 0.25260645291545814 1107 60-61 0.20040667824141423 1107 62-63 0.01740948993144542 1107 64-65 0.15119196581412808 1107 66-67 0.3589720822921194 1107 68-69 0.24323393698071527 1107 70-71 0.15402465000272514 1107 72-73 0.08309486508874642 1107 74-75 0.4120363344729405 1107 76-77 0.36373587101163807 1107 78-79 0.40836233829679713 1107 80-81 0.5774029088195896 1107 82-83 0.576931222911444 1107 84-85 0.6149361153269943 1107 86-87 0.39571084423443637 1107 88-89 0.45862929158740684 1107 90-91 0.3733590089720238 1107 92-93 0.36169126001693996 1107 94-95 0.5612143206869646 1107 96-97 0.5282768190925253 1107 98-99 0.3546907335411724 1107 100-101 0.7139649173541471 1108 1 0.25384133025542255 1108 2 0.30730991661684826 1108 3 0.24639657661168357 1108 4 0.16994026389819084 1108 5 0.2514746012000373 1108 6 0.10990127083601209 1108 7 0.14684971018842674 1108 8 0.2755331124794296 1108 9 0.2996217768609668 1108 10-11 0.29686551977012243 1108 12-13 0.32895313614034905 1108 14-15 0.41569137053830474 1108 16-17 0.4416863481422837 1108 18-19 0.40380916398073197 1108 20-21 0.3302726398294098 1108 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66-67 -0.87851319925376 2316 68-69 -1.0058710824377854 2316 70-71 -0.6104428959179558 2316 72-73 -0.8252297760677223 2316 74-75 -0.5719917905438585 2316 76-77 -0.8566739875299021 2316 78-79 -0.8920997808379951 2316 80-81 -0.6540832025155474 2316 82-83 -1.0631696421841816 2316 84-85 -1.0528802485472397 2316 86-87 -1.008534970527208 2316 88-89 -0.9281985656744993 2316 90-91 -0.6464349595533356 2316 92-93 -0.8061035974403197 2316 94-95 -0.6059304157070926 2316 96-97 -0.5955333831932457 2316 98-99 -0.6826318609212798 2316 100-101 -0.9543763375094372 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 14.0 4 50.0 5 86.0 6 154.0 7 485.0 8 1071.0 9 1919.0 10 3217.0 11 4873.0 12 6566.0 13 8546.0 14 10321.0 15 12565.0 16 14675.0 17 16627.0 18 15426.0 19 13950.0 20 14396.0 21 15688.0 22 17583.0 23 20826.0 24 25538.0 25 32284.0 26 41207.0 27 53080.0 28 67436.0 29 83666.0 30 102858.0 31 124538.0 32 148851.0 33 181263.0 34 221294.0 35 273739.0 36 351834.0 37 446389.0 38 386463.0 39 73682.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.484325069146735 22.143928336625354 15.187358644881567 23.18438794934635 2 15.184338520530094 22.212241259248252 40.301708319981955 22.3017119002397 3 18.04119355917421 27.157330782246557 31.22298212210416 23.578493536475076 4 12.86279888905403 18.377990233560094 39.20246112853057 29.5567497488553 5 12.592898105841725 38.68356250407252 35.98091592394725 12.742623466138511 6 29.666221533240012 39.23255655760301 18.080138337444552 13.021083571712428 7 25.58447012374108 33.263377000548836 23.873547305195423 17.27860557051466 8 24.30638868869826 37.29997196734464 21.077692701234547 17.315946642722555 9 25.329741716493547 16.327249566890103 21.362279645607092 36.98072907100926 10-11 23.909098297239947 27.151345191540365 29.57656369767551 19.362992813544174 12-13 24.364584194809414 24.630446900673107 29.562994890037164 21.441974014480316 14-15 21.67872375808199 26.34698681909565 26.816901015137105 25.15738840768525 16-17 21.449223839141965 29.29559271523297 27.58447298786934 21.670710457755717 18-19 21.418917781976113 28.462220895176127 29.312526873578452 20.806334449269308 20-21 22.072887768060028 27.608406359653596 29.18532377716939 21.133382095116986 22-23 21.87737689294434 27.437209749429726 29.031331264118148 21.654082093507785 24-25 21.76566737202234 27.703114060863467 28.94672634353966 21.584492223574536 26-27 21.71559803171821 27.793495103641263 28.838213938940875 21.652692925699654 28-29 21.747740735781544 27.978179599780322 28.79232741627243 21.4817522481657 30-31 22.139191794201977 27.659515809795515 28.662791197270842 21.538501198731662 32-33 21.905496691872134 27.797530972069634 28.533641347086284 21.76333098897195 34-35 22.023178109002746 27.78610584601745 28.724584546960546 21.466131498019255 36-37 22.075740719027547 27.680815280968872 28.68112049220458 21.562323507798997 38-39 21.954664357680027 27.65295529823108 28.728159248474256 21.66422109561464 40-41 21.940736794506748 27.604589024592396 28.818885402916262 21.635788777984597 42-43 21.971943442907627 27.67276659278965 28.45261069915823 21.90267926514449 44-45 21.9146843885224 27.797974568335 28.511471174325365 21.775869868817235 46-47 22.044052551131657 27.685246749210595 28.345447820699142 21.925252878958602 48-49 22.004903150777437 27.822856402203016 28.22763308935736 21.944607357662186 50-51 22.034930688182193 27.83033419760863 28.152661771816973 21.9820733423922 52-53 22.146524263762732 27.779061299944086 28.117946971072755 21.95646746522043 54-55 21.9532382988323 27.742196535931562 28.232014523735348 22.072550641500786 56-57 22.043030484118457 27.82961891853232 28.07386495770227 22.05348563964695 58-59 22.028029873321987 27.88208947100042 28.120810343938533 21.96907031173906 60-61 22.126037813708272 27.963442163624418 28.152306527310024 21.758213495357285 62-63 22.028446667873148 27.829642517340176 28.177270259471022 21.964640555315654 64-65 21.845500991274207 27.71213483049426 28.131544882573788 22.310819295657748 66-67 21.877955069239004 27.800735890079174 28.088830781300373 22.232478259381452 68-69 21.878178428509685 27.817893024892715 28.109743991134984 22.194184555462616 70-71 21.849794065776038 27.674614071541875 28.149946131183334 22.325645731498753 72-73 21.892791234822536 27.812929582581425 28.012537138684884 22.281742043911155 74-75 21.829048150499045 27.960995208297557 27.915448821240478 22.29450781996292 76-77 21.82512407259773 27.774827088405768 27.92714370874721 22.472905130249295 78-79 21.744047284448914 27.86103247118031 27.969429664274827 22.425490580095946 80-81 21.88247702674303 27.964093444687144 27.801104778530533 22.352324750039294 82-83 21.948584601515623 27.776709696613633 27.971846335222583 22.302859366648157 84-85 21.95811568934222 27.87907646151147 27.685013801523002 22.47779404762331 86-87 21.743620422809773 28.042769311295295 27.73385517750256 22.47975508839237 88-89 21.790810141609914 27.843726438027673 27.708360333757465 22.65710308660495 90-91 21.7999988900742 27.791933309929412 27.81860733313653 22.589460466859855 92-93 21.87816793554179 27.730265831755073 27.764015367587053 22.627550865116085 94-95 21.66454269891231 27.7642415483895 27.756239748252987 22.814976004445207 96-97 21.63557118414877 27.572586855584568 27.876184451292097 22.915657508974565 98-99 21.412598798999415 27.69876960630051 27.749554180535313 23.13907741416476 100-101 21.493832636813142 27.641451239469404 27.736468694541077 23.128247429176373 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 200.0 1 141.0 2 112.5 3 147.5 4 129.5 5 144.0 6 220.0 7 416.0 8 713.0 9 877.0 10 807.0 11 725.5 12 809.0 13 896.0 14 1037.5 15 1241.0 16 1402.5 17 1594.5 18 1845.0 19 2112.5 20 2457.5 21 2942.0 22 3624.5 23 4765.5 24 6473.5 25 9218.0 26 13535.5 27 19620.5 28 27712.0 29 38728.0 30 50550.5 31 61129.0 32 71855.5 33 78976.5 34 82711.5 35 85743.0 36 89161.0 37 92268.0 38 90977.5 39 88086.0 40 86575.5 41 85897.0 42 86189.5 43 88164.0 44 90588.5 45 93563.5 46 95510.0 47 96638.0 48 98300.0 49 98116.5 50 95641.5 51 92722.0 52 91169.5 53 89622.0 54 86353.5 55 79816.0 56 71949.0 57 63588.0 58 54898.5 59 47230.0 60 39555.0 61 32033.5 62 25279.0 63 19015.0 64 13714.0 65 9814.0 66 7054.5 67 5340.5 68 3942.0 69 2841.0 70 2044.5 71 1411.5 72 947.5 73 603.5 74 393.5 75 260.0 76 166.0 77 94.5 78 50.0 79 25.5 80 21.0 81 14.0 82 5.0 83 2.0 84 2.5 85 2.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020013096226475215 2 0.0027209218483222114 3 0.004045581169215919 4 0.03422633272363203 5 0.0020406913862416583 6 3.580160326739752E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 5.370240490109628E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.95040081684938E-5 24-25 6.265280571794566E-4 26-27 0.002685120245054814 28-29 1.790080163369876E-4 30-31 0.007840551115560057 32-33 0.023450050140145375 34-35 0.01455335172819709 36-37 8.413376767838417E-4 38-39 9.487424865860342E-4 40-41 0.0025777154352526212 42-43 0.008628186387442803 44-45 0.008431277569472116 46-47 0.00837757516457102 48-49 0.009773837691999524 50-51 0.19615698430207104 52-53 0.0491914028894042 54-55 0.017936603236966157 56-57 0.01040036574917898 58-59 0.020729128291823162 60-61 0.053165380852085324 62-63 0.09616310637622974 64-65 0.1010679260238632 66-67 0.10541782082085199 68-69 0.10300121260030266 70-71 0.09292306128053027 72-73 0.06959831675182078 74-75 0.25062912367341633 76-77 0.30470744540882033 78-79 0.007231923860014299 80-81 0.0011456513045567207 82-83 0.0 84-85 3.580160326739752E-5 86-87 0.0 88-89 1.790080163369876E-4 90-91 0.006856007025706626 92-93 0.01933286576439466 94-95 0.0017721793617361775 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2793171.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.3855410189428 #Duplication Level Percentage of deduplicated Percentage of total 1 78.7090708389846 47.528898257103805 2 12.164430602647823 14.691114462565466 3 3.792536146516777 6.870430411239363 4 1.6589059526741414 4.00695733807091 5 0.9533920835687673 2.8785548384738573 6 0.5916643137979227 2.1436781814173465 7 0.40922625279453195 1.7297944071906726 8 0.292833445882542 1.4146324846446885 9 0.20864081249836924 1.1338999507211245 >10 1.1415089133364913 12.187347385137281 >50 0.054387013568837295 2.2333810702170163 >100 0.021862174687992616 2.305201995554855 >500 0.0012450165333356736 0.49880801446991474 >1k 2.9643250790874757E-4 0.3773012031936294 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3544 0.1268808819796568 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3166 0.11334787594458055 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.1480961960438513E-4 3.580160326739752E-5 10-11 0.0 0.0 0.0 2.1480961960438513E-4 3.580160326739752E-5 12-13 0.0 0.0 0.0 2.1480961960438513E-4 1.9690881797068636E-4 14-15 0.0 0.0 0.0 2.1480961960438513E-4 2.3271042123808387E-4 16-17 0.0 0.0 0.0 2.1480961960438513E-4 2.685120245054814E-4 18-19 0.0 0.0 0.0 2.1480961960438513E-4 2.8641282613918017E-4 20-21 0.0 0.0 0.0 2.685120245054814E-4 3.5801603267397524E-4 22-23 0.0 0.0 0.0 2.8641282613918017E-4 4.117184375750715E-4 24-25 0.0 0.0 0.0 3.222144294065777E-4 4.2961923920877026E-4 26-27 0.0 0.0 0.0 4.47520040842469E-4 4.2961923920877026E-4 28-29 0.0 0.0 0.0 0.001432064130695901 4.2961923920877026E-4 30-31 0.0 0.0 0.0 0.00490481964763346 4.2961923920877026E-4 32-33 0.0 0.0 0.0 0.015663201429486413 4.654208424761678E-4 34-35 0.0 0.0 0.0 0.032865871799470925 5.549248506446615E-4 36-37 0.0 0.0 0.0 0.06082692395130839 5.728256522783603E-4 38-39 0.0 0.0 0.0 0.10210617251861773 6.086272555457579E-4 40-41 0.0 0.0 0.0 0.1484513479482638 6.086272555457579E-4 42-43 0.0 0.0 0.0 0.196515000334745 6.086272555457579E-4 44-45 0.0 0.0 0.0 0.25551604251941606 6.265280571794566E-4 46-47 0.0 0.0 0.0 0.32719085226074596 6.623296604468541E-4 48-49 0.0 0.0 0.0 0.4023205167173797 6.802304620805528E-4 50-51 0.0 0.0 0.0 0.48128095272362487 6.802304620805528E-4 52-53 0.0 0.0 0.0 0.5626579969504194 7.697344702490467E-4 54-55 0.0 0.0 0.0 0.6533971604316384 7.876352718827454E-4 56-57 0.0 0.0 0.0 0.7569532978825858 8.23436875150143E-4 58-59 0.0 0.0 0.0 0.8711067099006827 8.592384784175405E-4 60-61 0.0 0.0 0.0 0.9920982281428528 8.592384784175405E-4 62-63 0.0 0.0 0.0 1.1200352574188976 9.308416849523356E-4 64-65 0.0 0.0 0.0 1.261021971085909 9.308416849523356E-4 66-67 0.0 0.0 0.0 1.4249933140505897 9.487424865860343E-4 68-69 0.0 0.0 0.0 1.6032315959173284 9.66643288219733E-4 70-71 0.0 0.0 0.0 1.7939467365227548 9.66643288219733E-4 72-73 0.0 0.0 0.0 1.9928604442764155 9.66643288219733E-4 74-75 0.0 0.0 0.0 2.2070793374268884 9.845440898534317E-4 76-77 0.0 1.790080163369876E-5 0.0 2.4492413819275654 0.0010024448914871305 78-79 0.0 3.580160326739752E-5 0.0 2.7220316980235006 0.0010024448914871305 80-81 0.0 3.580160326739752E-5 0.0 3.018200461053047 0.0010382464947545282 82-83 0.0 3.580160326739752E-5 0.0 3.3262732571690026 0.0011456513045567207 84-85 0.0 3.580160326739752E-5 0.0 3.6437618749442837 0.001199353709457817 86-87 0.0 3.580160326739752E-5 0.0 3.995584230252999 0.0013067585192600094 88-89 0.0 3.580160326739752E-5 0.0 4.380057647741581 0.0013962625274285033 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3095 0.0 50.024185 1 GTATCAA 5905 0.0 41.580887 1 TCAACGC 7030 0.0 33.833126 4 CAACGCA 7060 0.0 33.742702 5 ATCAACG 7150 0.0 33.268276 3 AACGCAG 7405 0.0 32.362305 6 TATCAAC 7430 0.0 32.07961 2 ACGCAGA 8605 0.0 27.628672 7 CGCAGAG 8880 0.0 26.505856 8 TATACCG 155 1.4235738E-6 24.49284 5 GTACATG 8210 0.0 24.469229 1 TACATGG 8540 0.0 22.739433 2 ACATGGG 8360 0.0 22.660278 3 GCAGAGT 10370 0.0 21.965221 9 GTGGTAT 1590 0.0 21.505941 1 TGGTATC 1755 0.0 20.020216 2 CATGGGG 5970 0.0 19.482805 4 AGAGTAC 9560 0.0 18.663927 10-11 CAGAGTA 10150 0.0 18.046558 10-11 GAGTACT 6210 0.0 17.957645 12-13 >>END_MODULE