FastQCFastQC Report
Sat 18 Jun 2016
SRR1930952_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930952_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2874583
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50900.17706916098787198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19100.050.428351
GTATCAA35250.038.4247931
TCAACGC37550.033.1048244
ATCAACG38100.032.246253
CAACGCA38650.031.705775
TATCAAC43000.029.509352
AACGCAG42200.028.2516546
GTACATG57650.026.1923241
ACGCAGA50300.023.5316627
TACATGG61700.023.4107272
ACATGGG61650.022.6939053
CGCAGAG51950.022.4563628
GAGTACA44200.020.6564641
GTGGTAT11600.019.8932951
CATGGGG45000.019.8270024
GCGTACA2452.235578E-618.4282151
GCAGAGT59350.018.1985669
AGTACAT45750.017.9787852
CTAGTAC6050.017.406123
CAGAGTA55400.016.98355910-11
Produced by FastQC (version 0.11.5)