FastQCFastQC Report
Sat 18 Jun 2016
SRR1930952_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930952_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2874583
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26800.046.4240261
GTATCAA50500.039.682351
TCAACGC58350.033.1143724
CAACGCA58850.032.8210335
ATCAACG59500.032.5541343
TATCAAC64350.030.6918332
AACGCAG63300.030.6631186
ACGCAGA73200.026.1918987
CGCAGAG75900.025.0725968
GTACATG81050.022.557141
TACATGG84000.021.3643952
ACATGGG81100.021.0153453
GCAGAGT87400.021.0133979
CATGGGG57600.020.5229514
AGAGTAC79700.019.05409610-11
CAGAGTA86550.017.21707310-11
ATGGGGG33750.017.014375
GAGTACA64550.016.993861
GAGTACT45950.016.78280612-13
GTGGTAT16350.016.2647191
Produced by FastQC (version 0.11.5)