FastQCFastQC Report
Sat 18 Jun 2016
SRR1930951_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930951_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2392792
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42390.17715706170866502No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19400.055.4186821
GTATCAA34400.042.0608671
ATCAACG41800.033.2759633
TCAACGC41450.032.939334
CAACGCA41750.031.7902725
TATCAAC46350.030.6696262
AACGCAG45550.028.4136096
GTACATG48700.025.0681041
ACGCAGA52250.024.5170617
CGCAGAG54000.023.8409278
TACATGG50450.022.7010272
ACATGGG52750.021.6087933
CATGGGG35750.020.4996854
GCAGAGT58400.019.7519259
GTGGTAT9650.019.2627641
GTATACG2401.8095743E-618.8397641
GAGTACT32800.018.65544912-13
AGAGTAC54000.018.02562110-11
CAGAGTA57500.017.92872610-11
GTATTAG6050.017.438461
Produced by FastQC (version 0.11.5)