FastQCFastQC Report
Sat 18 Jun 2016
SRR1930951_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930951_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2392792
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30960.12938859708658337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27660.11559717685448631No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25750.052.93651
GTATCAA45450.039.1875341
CAACGCA56600.031.1892435
ATCAACG56750.030.8714263
TCAACGC57200.030.791994
AACGCAG61050.029.070686
TATCAAC60450.029.0622252
ACGCAGA69300.025.1990327
GTACATG62700.025.1490251
CGCAGAG72100.024.1546128
TACATGG65150.023.7589152
GTGGTAT11550.023.4392361
ACATGGG64200.023.2215293
CATGGGG44150.020.8607964
GCAGAGT79550.020.759099
AGAGTAC73150.018.7154710-11
CAGAGTA77950.017.92827210-11
GAGTACT47800.017.86955812-13
GAGTACA48800.017.713411
AGTACTT55350.016.67520712-13
Produced by FastQC (version 0.11.5)