##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930951_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2392792 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.254435822252834 31.0 31.0 33.0 30.0 34.0 2 31.384332194357054 31.0 31.0 34.0 30.0 34.0 3 31.463919972985533 31.0 31.0 34.0 30.0 34.0 4 35.33608479132327 37.0 35.0 37.0 33.0 37.0 5 35.119365160030625 37.0 35.0 37.0 32.0 37.0 6 35.14983124316698 37.0 35.0 37.0 32.0 37.0 7 35.02235004129068 36.0 35.0 37.0 32.0 37.0 8 35.000704198275486 36.0 35.0 37.0 32.0 37.0 9 36.505250351890176 38.0 35.0 39.0 32.0 39.0 10-11 36.431240575862844 38.0 35.0 39.0 32.0 39.0 12-13 36.337641132200375 38.0 35.0 39.0 32.0 39.0 14-15 37.363519060578604 39.0 36.0 41.0 32.0 41.0 16-17 37.3290327784446 39.0 36.0 41.0 32.0 41.0 18-19 37.31715982835115 39.0 36.0 40.0 32.0 41.0 20-21 37.28262799273819 39.0 36.0 40.0 31.0 41.0 22-23 37.152828996419245 39.0 36.0 40.0 31.0 41.0 24-25 37.04016625766051 39.0 36.0 40.0 31.0 41.0 26-27 36.78139867568932 39.0 35.5 40.0 30.5 41.0 28-29 36.596917951915586 38.0 35.0 40.0 30.0 41.0 30-31 36.37274238630019 38.0 35.0 40.0 30.0 41.0 32-33 36.152860967438876 38.0 35.0 40.0 30.0 41.0 34-35 36.35378064620744 38.0 35.0 40.0 30.0 41.0 36-37 36.414883324584835 38.0 35.0 40.0 30.0 41.0 38-39 36.38387310723205 38.0 35.0 40.0 30.0 41.0 40-41 36.3010687932758 38.0 35.0 40.0 30.0 41.0 42-43 36.17008728715241 38.0 35.0 40.0 30.0 41.0 44-45 35.9995707942855 38.0 35.0 40.0 29.0 41.0 46-47 35.80490782316223 38.0 34.5 40.0 28.5 41.0 48-49 35.63014294598109 38.0 34.0 40.0 28.0 41.0 50-51 35.36646436464181 38.0 34.0 40.0 27.5 41.0 52-53 35.16110489336307 38.0 34.0 40.0 27.0 41.0 54-55 35.00168673248657 38.0 33.5 40.0 26.5 41.0 56-57 34.74647608316978 38.0 33.0 40.0 26.0 41.0 58-59 34.5089086305872 37.0 33.0 40.0 26.0 41.0 60-61 34.20436836967025 37.0 33.0 40.0 26.0 41.0 62-63 33.90310189937111 36.5 32.0 39.5 25.0 41.0 64-65 33.597591641897836 36.0 32.0 39.0 24.5 41.0 66-67 33.26419283414522 36.0 32.0 39.0 23.5 40.0 68-69 32.89768082641534 35.0 31.0 39.0 23.0 40.0 70-71 32.5099578233294 35.0 31.0 38.0 22.5 40.0 72-73 32.10007660507056 35.0 31.0 37.5 22.0 40.0 74-75 31.57616729745001 34.5 30.0 37.0 20.5 39.0 76-77 30.175959506718513 33.5 28.5 35.5 19.5 38.0 78-79 30.951450439486592 34.0 30.0 36.0 20.0 39.0 80-81 30.952622919167233 34.0 30.0 36.0 20.0 37.5 82-83 30.68361646143919 34.0 30.0 35.0 20.0 37.0 84-85 30.369931862025616 34.0 30.0 35.0 18.5 37.0 86-87 30.041342498637576 34.0 30.0 35.0 18.0 36.0 88-89 29.704763723716894 34.0 29.0 35.0 16.0 36.0 90-91 29.378513050862757 34.0 29.0 35.0 9.5 35.5 92-93 29.038838937943623 34.0 29.0 35.0 4.5 35.0 94-95 28.642422951932303 33.5 29.0 35.0 2.0 35.0 96-97 28.197248026573142 33.0 29.0 35.0 2.0 35.0 98-99 27.72770867672577 33.0 27.5 35.0 2.0 35.0 100-101 26.54602364100181 32.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4721392004945173 1101 2 0.5354988766831994 1101 3 0.550834533601769 1101 4 0.3584608381091172 1101 5 0.49686829659405873 1101 6 0.42251617604953395 1101 7 0.4622498962720698 1101 8 0.47029582317977514 1101 9 0.58542820144568 1101 10-11 0.5716488305248859 1101 12-13 0.5760802508046297 1101 14-15 0.8047602549948678 1101 16-17 0.8500861240268804 1101 18-19 0.7726123751873146 1101 20-21 0.7472591955134149 1101 22-23 0.7710999117553357 1101 24-25 0.7593874057415491 1101 26-27 0.8576742817143526 1101 28-29 0.8173379230859155 1101 30-31 0.8516450444693291 1101 32-33 0.8977034906502226 1101 34-35 0.8639674243600481 1101 36-37 0.7406261232953071 1101 38-39 0.9312902400399992 1101 40-41 0.7998471154696531 1101 42-43 0.8684345667742974 1101 44-45 0.9218573795854041 1101 46-47 1.0619438744137852 1101 48-49 1.008830856924611 1101 50-51 1.0192479536359897 1101 52-53 1.0170389271682652 1101 54-55 0.9314140320889379 1101 56-57 0.9627964430039668 1101 58-59 0.9349976147343568 1101 60-61 0.8697414434965864 1101 62-63 0.9936120053745796 1101 64-65 0.9111209602750705 1101 66-67 0.9391672099504547 1101 68-69 0.8666258096303352 1101 70-71 0.8701089831979942 1101 72-73 0.8412233696279614 1101 74-75 0.9140132707098836 1101 76-77 0.9213532725735583 1101 78-79 0.9909491113158175 1101 80-81 0.8513737685297116 1101 82-83 1.1823296528436913 1101 84-85 1.2057954427043605 1101 86-87 1.0528645804672543 1101 88-89 0.8665416007590032 1101 90-91 0.9616330156855462 1101 92-93 1.0592670051064381 1101 94-95 0.9409201335970678 1101 96-97 1.0736764262283742 1101 98-99 1.1931521568734347 1101 100-101 1.287570462232754 1102 1 0.4347772631385496 1102 2 0.44601269251800346 1102 3 0.4280271130935418 1102 4 0.22731951004333695 1102 5 0.3911932768790223 1102 6 0.32567716555271176 1102 7 0.3634969581581302 1102 8 0.4926182921908051 1102 9 0.6214395634289716 1102 10-11 0.46550049692357476 1102 12-13 0.5011274520357603 1102 14-15 0.625547554686321 1102 16-17 0.5958427601448193 1102 18-19 0.639345728427088 1102 20-21 0.5982968569661224 1102 22-23 0.5828314657592202 1102 24-25 0.5776379359419082 1102 26-27 0.6236866390196383 1102 28-29 0.7512297086169966 1102 30-31 0.7131234804356694 1102 32-33 0.6993521266762883 1102 34-35 0.7710512840655852 1102 36-37 0.7011440735445191 1102 38-39 0.6821310574290322 1102 40-41 0.6723711754646047 1102 42-43 0.6359150240099041 1102 44-45 0.7102598112335841 1102 46-47 0.7981118082019094 1102 48-49 0.6475436759061708 1102 50-51 0.6876274928784696 1102 52-53 0.7576512033165272 1102 54-55 0.5361170816023844 1102 56-57 0.5450885164127897 1102 58-59 0.5512330772911085 1102 60-61 0.5383294644136569 1102 62-63 0.40002065395701436 1102 64-65 0.3680222932408981 1102 66-67 0.37748886739125 1102 68-69 0.41073832695276025 1102 70-71 0.3343270812142478 1102 72-73 0.37272684177042237 1102 74-75 0.5612141190304705 1102 76-77 0.7015747699972081 1102 78-79 0.7096645334471852 1102 80-81 0.6585099974843942 1102 82-83 0.6552171584902311 1102 84-85 0.7445071132374679 1102 86-87 0.6986973969987851 1102 88-89 0.6385739716925407 1102 90-91 0.578707917493201 1102 92-93 0.5935943588515258 1102 94-95 0.7657312903909066 1102 96-97 0.7200610339566644 1102 98-99 0.6033713574001922 1102 100-101 0.9441917269041831 1103 1 0.37604568355019197 1103 2 0.3528988817763157 1103 3 0.41504909579966665 1103 4 0.2267257942961649 1103 5 0.2725271582576667 1103 6 0.34982038805910776 1103 7 0.45252021963663225 1103 8 0.3574416998147285 1103 9 0.48871608273234557 1103 10-11 0.4902214505224052 1103 12-13 0.49651519408910616 1103 14-15 0.6098628025245816 1103 16-17 0.655291822378409 1103 18-19 0.6016443217774707 1103 20-21 0.6844877898610235 1103 22-23 0.5876678133417315 1103 24-25 0.6824681941321415 1103 26-27 0.7253700515150712 1103 28-29 0.741626432523951 1103 30-31 0.7821204052925665 1103 32-33 0.8059805824064128 1103 34-35 0.821143538358605 1103 36-37 0.7341039748963638 1103 38-39 0.795697310017033 1103 40-41 0.737627843349145 1103 42-43 0.758534528333378 1103 44-45 0.7044522055826263 1103 46-47 0.7695976199179029 1103 48-49 0.7281008552996653 1103 50-51 0.5973635177231955 1103 52-53 0.5701720594850812 1103 54-55 0.6444860378805402 1103 56-57 0.7554922431208695 1103 58-59 0.7187706792884327 1103 60-61 0.7645222241262388 1103 62-63 0.6443343794472085 1103 64-65 0.6671565338936816 1103 66-67 0.531812488143899 1103 68-69 0.7032363479002726 1103 70-71 0.5517386327913911 1103 72-73 0.5481683227106302 1103 74-75 0.534886631513718 1103 76-77 0.7816909251549227 1103 78-79 0.7200793732961692 1103 80-81 0.6903961867427029 1103 82-83 0.5318692106154259 1103 84-85 0.6689969743993238 1103 86-87 0.7215332269829524 1103 88-89 0.42700397412829716 1103 90-91 0.48758533802663706 1103 92-93 0.5106531823809384 1103 94-95 0.5288991444890634 1103 96-97 0.48240697233201857 1103 98-99 0.5727969754735085 1103 100-101 0.7635281353806143 1104 1 0.501460986608862 1104 2 0.5707868020855251 1104 3 0.5256025034175451 1104 4 0.29573749150527817 1104 5 0.483281463546291 1104 6 0.5266529942664917 1104 7 0.5056476116282127 1104 8 0.4737872817248103 1104 9 0.5898075714486666 1104 10-11 0.6731262347425471 1104 12-13 0.6974696653804671 1104 14-15 0.8006689347911973 1104 16-17 0.7079035661535968 1104 18-19 0.7944914967687637 1104 20-21 0.8093360502092395 1104 22-23 0.7855660155119679 1104 24-25 0.7654631186666592 1104 26-27 0.824762115375762 1104 28-29 0.6961508957106872 1104 30-31 0.8993818311624509 1104 32-33 0.9117771852155343 1104 34-35 0.9363606630632759 1104 36-37 1.0192586234468948 1104 38-39 0.9258917085799965 1104 40-41 1.028154105195057 1104 42-43 1.0195562775353508 1104 44-45 1.0936838149441854 1104 46-47 1.1863865274753493 1104 48-49 1.033385631154509 1104 50-51 1.0731978858582565 1104 52-53 1.0219926835179152 1104 54-55 1.0422472818702033 1104 56-57 1.105657001723742 1104 58-59 1.1385449848851223 1104 60-61 1.0736030091615802 1104 62-63 1.0363603237432741 1104 64-65 1.2187098769351934 1104 66-67 1.0923976359349155 1104 68-69 0.9575968078498889 1104 70-71 0.9895335957162672 1104 72-73 1.0054205449670803 1104 74-75 1.1114490241332966 1104 76-77 1.1836268979830855 1104 78-79 1.2717420222069045 1104 80-81 1.1366797575669807 1104 82-83 1.219397796025671 1104 84-85 1.3718879961514894 1104 86-87 1.3012284620764767 1104 88-89 1.3112208434897141 1104 90-91 1.2200888452935494 1104 92-93 1.2880282300726584 1104 94-95 1.341544219148826 1104 96-97 1.3501203587167119 1104 98-99 1.469520035871959 1104 100-101 1.7209042524332752 1105 1 0.21446449999648465 1105 2 0.32042355502251496 1105 3 0.3298525672015664 1105 4 0.1516248149874002 1105 5 0.26339615189406373 1105 6 0.29065461051843045 1105 7 0.28045052486088196 1105 8 0.30847387982463204 1105 9 0.357076216863355 1105 10-11 0.3154224565049546 1105 12-13 0.2207607071783073 1105 14-15 0.4928577916645409 1105 16-17 0.37666752256512126 1105 18-19 0.34225848555447413 1105 20-21 0.386015340064894 1105 22-23 0.4741804071762701 1105 24-25 0.4430591136154334 1105 26-27 0.5219038891098577 1105 28-29 0.4529196821524266 1105 30-31 0.502067152996716 1105 32-33 0.43724653904246225 1105 34-35 0.48999570846111595 1105 36-37 0.6067521572688079 1105 38-39 0.4527880103942721 1105 40-41 0.5067103129600881 1105 42-43 0.45880221876510774 1105 44-45 0.45160739934858896 1105 46-47 0.40322849291260354 1105 48-49 0.5764294572125621 1105 50-51 0.5667839345962662 1105 52-53 0.5173567849356786 1105 54-55 0.6071439371298695 1105 56-57 0.6424811544495981 1105 58-59 0.5736855613522138 1105 60-61 0.6018324116048106 1105 62-63 0.46642026901776035 1105 64-65 0.4142712406726332 1105 66-67 0.36905599117328336 1105 68-69 0.44173089882814054 1105 70-71 0.3244613588516714 1105 72-73 0.28504339415825797 1105 74-75 0.4576195984001252 1105 76-77 0.45033589714745403 1105 78-79 0.5007077789662517 1105 80-81 0.6563871176576157 1105 82-83 0.6095268240742513 1105 84-85 0.6057503467271808 1105 86-87 0.5812017576387483 1105 88-89 0.6051229010802466 1105 90-91 0.49211868977758044 1105 92-93 0.5315504216447415 1105 94-95 0.550142904729892 1105 96-97 0.3594337032697901 1105 98-99 0.6452829717391779 1105 100-101 0.8231059275537653 1106 1 0.23935863682359937 1106 2 0.23384901360495647 1106 3 0.23774242447881377 1106 4 0.09646044567598722 1106 5 0.08218366608221572 1106 6 0.1693578962459199 1106 7 0.1500886469773377 1106 8 0.28623654134446497 1106 9 0.21767612989797414 1106 10-11 0.3028012944152252 1106 12-13 0.2627837808477054 1106 14-15 0.2721849118307986 1106 16-17 0.31965935978583815 1106 18-19 0.2852792026278905 1106 20-21 0.2899893114682115 1106 22-23 0.3876548134065061 1106 24-25 0.32476582997321657 1106 26-27 0.2771840147949689 1106 28-29 0.2815089442973644 1106 30-31 0.3223757468115096 1106 32-33 0.42956161023796113 1106 34-35 0.37835389920201834 1106 36-37 0.456592918204052 1106 38-39 0.3853253409361841 1106 40-41 0.4041795048218333 1106 42-43 0.5054792436722764 1106 44-45 0.4101668841438624 1106 46-47 0.326528639592631 1106 48-49 0.49102492008402265 1106 50-51 0.185008721151938 1106 52-53 0.31098476385545837 1106 54-55 0.3630725265845456 1106 56-57 0.5488544492053791 1106 58-59 0.5164185906621626 1106 60-61 0.3270543865957407 1106 62-63 0.19589868999182158 1106 64-65 0.2133698042035661 1106 66-67 0.234888044906981 1106 68-69 0.22030277382073393 1106 70-71 0.15880689399683234 1106 72-73 0.1533007631118437 1106 74-75 0.4341459800715235 1106 76-77 0.3922154897845438 1106 78-79 0.34137289234115187 1106 80-81 0.32419490876060664 1106 82-83 0.3926190624731447 1106 84-85 0.4570327306686828 1106 86-87 0.38930676241355044 1106 88-89 0.5104331951038041 1106 90-91 0.3868007537006868 1106 92-93 0.5405875199630152 1106 94-95 0.3790494009491461 1106 96-97 0.3518511868660319 1106 98-99 0.2648663702373071 1106 100-101 0.661328027542627 1107 1 0.11241654759804476 1107 2 0.21628651458972215 1107 3 0.12151533567956463 1107 4 -0.02955968082478222 1107 5 0.06728456585363318 1107 6 0.1632103000417331 1107 7 0.2624037629692637 1107 8 0.058651198681651806 1107 9 0.1097620201280094 1107 10-11 0.2024169150348527 1107 12-13 0.08289193365761349 1107 14-15 0.12386148948631615 1107 16-17 0.14880292400449235 1107 18-19 0.12344094943581041 1107 20-21 0.24580165987852354 1107 22-23 0.2417872341130689 1107 24-25 0.13753148695743533 1107 26-27 0.24768385818941852 1107 28-29 0.26131978658489174 1107 30-31 0.13117435766799446 1107 32-33 0.23618997885871806 1107 34-35 0.2792809716707083 1107 36-37 0.16687684473153297 1107 38-39 0.28461803163160937 1107 40-41 0.1729780243803063 1107 42-43 0.22035082196614297 1107 44-45 0.294732836432992 1107 46-47 0.28096268244409117 1107 48-49 0.22905070647287573 1107 50-51 0.16019325727039302 1107 52-53 0.15321884402130337 1107 54-55 0.19263289570537268 1107 56-57 0.19749034637847274 1107 58-59 0.2717103783907291 1107 60-61 0.19389261990841788 1107 62-63 -0.09508613141820632 1107 64-65 -0.08668172027454091 1107 66-67 0.08777726639553407 1107 68-69 0.12510191302768447 1107 70-71 0.11785163400142551 1107 72-73 0.1231802217499407 1107 74-75 0.2891924853674688 1107 76-77 0.30919790770063216 1107 78-79 0.21557364814148272 1107 80-81 0.31965305325798 1107 82-83 0.2944318729692341 1107 84-85 0.3826118877435327 1107 86-87 0.42815767672638216 1107 88-89 0.44621034076552135 1107 90-91 0.4457579948630759 1107 92-93 0.5802300028155543 1107 94-95 0.6517424436131556 1107 96-97 0.45649947818681724 1107 98-99 0.49914614005438906 1107 100-101 0.903370484949523 1108 1 0.26382898991350245 1108 2 0.31838674042109716 1108 3 0.3285425454755426 1108 4 0.13066446737104087 1108 5 0.26567463260384017 1108 6 0.04199633596658714 1108 7 0.1840078021213003 1108 8 0.21080189012295847 1108 9 0.25148667119563584 1108 10-11 0.24346966274902826 1108 12-13 0.2119258369123358 1108 14-15 0.35003671072314546 1108 16-17 0.35341762784540975 1108 18-19 0.3267532717876449 1108 20-21 0.4190455339720316 1108 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-0.6453082873643048 2316 80-81 -0.9512686862663351 2316 82-83 -0.9461825697178874 2316 84-85 -0.9478865967217622 2316 86-87 -0.619472861203505 2316 88-89 -0.7918851624561682 2316 90-91 -0.51952446456853 2316 92-93 -0.8202874116784677 2316 94-95 -0.6292764750915261 2316 96-97 -0.48836742653644194 2316 98-99 -0.6389631407555072 2316 100-101 -0.9298298223095642 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 9.0 4 29.0 5 65.0 6 137.0 7 385.0 8 964.0 9 1770.0 10 2848.0 11 4288.0 12 5629.0 13 7307.0 14 8811.0 15 10557.0 16 12230.0 17 14158.0 18 13037.0 19 11999.0 20 12351.0 21 13716.0 22 15277.0 23 17750.0 24 21745.0 25 27196.0 26 34825.0 27 45242.0 28 57269.0 29 71806.0 30 88207.0 31 106372.0 32 127942.0 33 156200.0 34 190650.0 35 236139.0 36 303384.0 37 385563.0 38 329103.0 39 57824.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.41726875819015 22.10499695906335 14.95196156088875 23.525772721857752 2 15.399706025065187 22.201836319403725 39.654560644554884 22.74389701097621 3 17.917752959634623 26.449809294460554 31.516399506163346 24.11603823974148 4 13.042596671131692 18.298491089181763 38.58596677820867 30.07294546147787 5 12.880420336784319 38.39025254644451 35.77451532714686 12.954811789624316 6 30.150243021744487 38.68325260469995 17.859820544218255 13.3066838293373 7 25.946718310659683 33.33682994593764 23.295129706217672 17.421322037185014 8 24.578066125262872 36.58972447249907 21.15666551877472 17.675543883463334 9 25.352485862743553 16.13977150532579 21.31326973396339 37.194472897967266 10-11 23.972998906716505 26.90319509593813 29.388074684301852 19.73573131304351 12-13 24.420697661978142 24.475508109355097 29.27368948074049 21.83010474792627 14-15 21.879900133400646 26.35034720945239 26.45750236543753 25.312250291709432 16-17 21.582298001665002 29.26723258854092 27.397554822985033 21.752914586809048 18-19 21.575005265814998 28.321538186353013 29.029351485628503 21.074105062203486 20-21 22.329312368145665 27.599431960655167 28.76146359566565 21.30979207553352 22-23 22.198198672596146 27.399739467425277 28.581288545468453 21.820773314510124 24-25 22.07388015741519 27.53907013200751 28.59798464589627 21.789065064681026 26-27 22.046646649114916 27.700159670937026 28.524112693703668 21.72908098624439 28-29 21.988044077434324 27.757399419673025 28.55103396078802 21.70352254210463 30-31 22.24971657550063 27.571660684711794 28.401381335255916 21.777241404531658 32-33 22.13982813939883 27.58949339935821 28.33691332708672 21.933765134156232 34-35 22.217173935850724 27.56123322901911 28.394771721552498 21.826821113577665 36-37 22.28900431047813 27.453741974795783 28.406778074318762 21.85047564040733 38-39 22.147098028505354 27.49761417531961 28.446114005119988 21.909173791055043 40-41 22.249162518527406 27.42816340851146 28.482582412335315 21.84009166062582 42-43 22.29202619940959 27.414001579053178 28.22799973083371 22.065972490703523 44-45 22.217887066428403 27.4872329429673 28.179100123360374 22.115779867243923 46-47 22.289090911370682 27.47649715809232 27.984316531547705 22.25009539898929 48-49 22.281396600784433 27.41758590826866 28.022922803767692 22.278094687179212 50-51 22.213754267011936 27.48282307854652 27.91337672648579 22.390045927955754 52-53 22.27962690210205 27.515771362028936 27.935093593299037 22.269508142569975 54-55 22.204631495523873 27.452715081641177 27.871214144699856 22.471439278135097 56-57 22.279783433865177 27.55078263533659 27.75247327562444 22.41696065517379 58-59 22.136683915753892 27.55080308681957 27.955810735315232 22.356702262111302 60-61 22.197338012085197 27.759279554726884 27.829444106909367 22.213938326278548 62-63 22.143537963766104 27.63543054758708 27.768269060423222 22.452762428223593 64-65 21.928362750772312 27.597980329992104 27.79715280829638 22.676504110939195 66-67 22.076630320870226 27.535256315052496 27.69739206394562 22.69072130013166 68-69 22.02249644714774 27.45322513641405 27.815852435622983 22.708425980815225 70-71 21.98917126299918 27.254559399410226 27.823249010126606 22.933020327463986 72-73 22.0730678624108 27.321135003410497 27.68560444004488 22.92019269413382 74-75 21.997570764371265 27.51501094161581 27.523935177368138 22.963483116644788 76-77 21.890833818552693 27.3577364087228 27.62256347570487 23.12886629701964 78-79 21.893322706159406 27.373993697673484 27.568342250727607 23.164341345439507 80-81 21.922100262123198 27.43454442720732 27.50147527884024 23.141880031829245 82-83 21.92388640550453 27.209782546915907 27.687070167402766 23.1792608801768 84-85 21.944239198392506 27.151670517119747 27.478924202354406 23.42516608213334 86-87 21.71741212775703 27.268855796910053 27.610193447654456 23.40353862767846 88-89 21.806519505062923 27.040599902456925 27.52683165962398 23.62604893285618 90-91 21.778505011749637 27.013189474751087 27.573619378695597 23.634686134803683 92-93 21.805409662219017 26.847357973272295 27.61572412720719 23.731508237301497 94-95 21.571505022788685 26.77467234800874 27.694808200374087 23.959014428828485 96-97 21.56284374070124 26.653445013189614 27.656332017158196 24.12737922895095 98-99 21.449210796425263 26.65616150505351 27.625761035643713 24.268866662877507 100-101 21.428356497347032 26.576505605167522 27.587270435541413 24.40786746194404 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 247.0 1 178.0 2 134.5 3 181.0 4 186.5 5 175.5 6 260.5 7 508.0 8 795.0 9 917.5 10 786.0 11 653.5 12 691.5 13 740.5 14 794.5 15 892.5 16 1035.0 17 1197.0 18 1337.5 19 1514.5 20 1765.0 21 2036.0 22 2438.0 23 3025.5 24 3755.5 25 4847.5 26 6676.5 27 9628.5 28 13936.5 29 20712.0 30 29606.0 31 39287.5 32 50125.0 33 59778.5 34 67770.0 35 73829.5 36 79756.5 37 84893.5 38 84833.0 39 82389.0 40 80245.5 41 78847.0 42 78482.5 43 79961.5 44 81726.5 45 83843.0 46 86101.0 47 86898.0 48 87492.0 49 86824.5 50 84442.5 51 81814.5 52 79897.0 53 78298.5 54 75240.5 55 69472.5 56 62350.5 57 54774.5 58 47805.5 59 41952.0 60 35406.5 61 28764.5 62 23092.5 63 17729.5 64 13007.0 65 9515.5 66 6858.0 67 5152.0 68 3753.0 69 2564.5 70 1904.0 71 1356.0 72 942.5 73 680.0 74 492.0 75 356.5 76 229.0 77 137.5 78 74.5 79 33.5 80 23.0 81 21.5 82 15.0 83 9.0 84 5.0 85 2.0 86 2.5 87 1.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018263183761898234 2 0.002967244959026944 3 0.004095633887107613 4 0.03627561442866743 5 0.0018388560309462755 6 8.358436504301252E-5 7 0.0 8 0.0 9 4.179218252150626E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.179218252150626E-5 24-25 4.806100989973219E-4 26-27 0.002444842677508116 28-29 1.2537654756451878E-4 30-31 0.007501696762610373 32-33 0.02350810266834727 34-35 0.014251134239833632 36-37 7.522592853871126E-4 38-39 5.641944640403344E-4 40-41 0.0021940895823790783 42-43 0.00915248797220987 44-45 0.008713670055734054 46-47 0.008734566146994808 48-49 0.010030123805161502 50-51 0.19659042658116543 52-53 0.04992076202193922 54-55 0.01809601503181221 56-57 0.011137616641981419 58-59 0.020540857709320324 60-61 0.05311786398483445 62-63 0.09593395497811762 64-65 0.10036392632539728 66-67 0.10494017031150221 68-69 0.10276697682038388 70-71 0.09229803509874657 72-73 0.06874814024787779 74-75 0.25213223715224725 76-77 0.30395454347891504 78-79 0.007668865492696399 80-81 0.0010030123805161502 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.2537654756451878E-4 90-91 0.006874814024787779 92-93 0.01851393685702727 94-95 0.0019851286697715473 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2392792.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.529502521670885 #Duplication Level Percentage of deduplicated Percentage of total 1 79.5759573491365 49.75845025727207 2 12.035085992384314 15.050958798186418 3 3.5831729972627935 6.7216207490378075 4 1.5408116167006485 3.85384735487612 5 0.8645541482483532 2.7030070396508217 6 0.5343468672720261 2.004746627071986 7 0.3534673774326142 1.5471497487950732 8 0.26080967638109126 1.304663945355809 9 0.18892831038885882 1.0632233934787652 >10 0.9885346706361342 10.990813392411319 >50 0.053267117601370324 2.2644032266258685 >100 0.020528333148206714 2.194035782343409 >500 2.0082877767553712E-4 0.083801875224289 >1k 3.347146294587171E-4 0.4592778096701981 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3096 0.12938859708658337 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2766 0.11559717685448631 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.179218252150626E-5 2 0.0 0.0 0.0 0.0 4.179218252150626E-5 3 0.0 0.0 0.0 0.0 4.179218252150626E-5 4 0.0 0.0 0.0 0.0 4.179218252150626E-5 5 0.0 0.0 0.0 0.0 4.179218252150626E-5 6 0.0 0.0 0.0 0.0 4.179218252150626E-5 7 0.0 0.0 0.0 0.0 8.358436504301252E-5 8 0.0 0.0 0.0 0.0 8.358436504301252E-5 9 0.0 0.0 0.0 0.0 8.358436504301252E-5 10-11 0.0 0.0 4.179218252150626E-5 0.0 8.358436504301252E-5 12-13 0.0 2.089609126075313E-5 4.179218252150626E-5 0.0 1.2537654756451878E-4 14-15 0.0 4.179218252150626E-5 4.179218252150626E-5 0.0 2.2985700386828441E-4 16-17 0.0 4.179218252150626E-5 4.179218252150626E-5 2.089609126075313E-5 2.5075309512903756E-4 18-19 0.0 4.179218252150626E-5 4.179218252150626E-5 4.179218252150626E-5 3.343374601720501E-4 20-21 0.0 8.358436504301252E-5 4.179218252150626E-5 8.358436504301252E-5 4.179218252150626E-4 22-23 0.0 1.2537654756451878E-4 4.179218252150626E-5 1.6716873008602504E-4 5.224022815188282E-4 24-25 0.0 1.2537654756451878E-4 4.179218252150626E-5 4.388179164758157E-4 5.641944640403344E-4 26-27 0.0 1.2537654756451878E-4 4.179218252150626E-5 0.0010239084717769034 5.850905553010876E-4 28-29 0.0 1.2537654756451878E-4 4.179218252150626E-5 0.0028418684114624258 5.850905553010876E-4 30-31 0.0 1.2537654756451878E-4 4.179218252150626E-5 0.011054032276938405 5.850905553010876E-4 32-33 0.0 1.2537654756451878E-4 4.179218252150626E-5 0.03556514732580182 6.47778829083347E-4 34-35 0.0 1.2537654756451878E-4 4.179218252150626E-5 0.07278108586120315 6.686749203441002E-4 36-37 0.0 1.2537654756451878E-4 4.179218252150626E-5 0.13333795833486572 6.686749203441002E-4 38-39 0.0 1.2537654756451878E-4 4.179218252150626E-5 0.21681784292157447 6.686749203441002E-4 40-41 0.0 1.2537654756451878E-4 4.179218252150626E-5 0.3050202441332134 6.686749203441002E-4 42-43 0.0 1.2537654756451878E-4 4.179218252150626E-5 0.40045269292107294 6.686749203441002E-4 44-45 0.0 1.2537654756451878E-4 4.179218252150626E-5 0.5204171528490567 6.895710116048533E-4 46-47 0.0 1.2537654756451878E-4 4.179218252150626E-5 0.659292575368022 7.104671028656063E-4 48-49 0.0 1.2537654756451878E-4 4.179218252150626E-5 0.8004456718344093 8.776358329516314E-4 50-51 0.0 1.2537654756451878E-4 4.179218252150626E-5 0.9438555461569581 8.776358329516314E-4 52-53 0.0 1.2537654756451878E-4 4.179218252150626E-5 1.0914028465491359 0.0010865967455591626 54-55 0.0 1.2537654756451878E-4 4.179218252150626E-5 1.2639209759979138 0.0011701811106021752 56-57 0.0 1.2537654756451878E-4 4.179218252150626E-5 1.4626219077964153 0.0011701811106021752 58-59 0.0 1.2537654756451878E-4 4.179218252150626E-5 1.673818702168847 0.0011701811106021752 60-61 0.0 1.2537654756451878E-4 4.179218252150626E-5 1.8919321027485883 0.0011910772018629283 62-63 0.0 1.2537654756451878E-4 4.179218252150626E-5 2.113200813108703 0.0012537654756451877 64-65 0.0 1.2537654756451878E-4 4.179218252150626E-5 2.3596702095292863 0.0012537654756451877 66-67 0.0 1.6716873008602504E-4 4.179218252150626E-5 2.6447346865084804 0.0012746615669059409 68-69 0.0 1.6716873008602504E-4 4.179218252150626E-5 2.9579879905984305 0.001295557658166694 70-71 0.0 1.6716873008602504E-4 4.179218252150626E-5 3.280351990478069 0.001295557658166694 72-73 0.0 1.6716873008602504E-4 4.179218252150626E-5 3.6238001464398075 0.0015254146620349784 74-75 0.0 1.6716873008602504E-4 4.179218252150626E-5 3.9907564050698934 0.0015881029358172378 76-77 0.0 1.6716873008602504E-4 4.179218252150626E-5 4.406108011059883 0.0015881029358172378 78-79 0.0 1.6716873008602504E-4 4.179218252150626E-5 4.863189111297597 0.001608999027077991 80-81 0.0 1.6716873008602504E-4 4.179218252150626E-5 5.340748380970849 0.001629895118338744 82-83 0.0 1.6716873008602504E-4 4.179218252150626E-5 5.841711272856145 0.0016716873008602504 84-85 0.0 2.089609126075313E-4 4.179218252150626E-5 6.366641981417525 0.0016716873008602504 86-87 0.0 2.089609126075313E-4 4.179218252150626E-5 6.942371087833794 0.0017552716659032627 88-89 0.0 2.089609126075313E-4 4.179218252150626E-5 7.566286580697361 0.0018806482134677816 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2575 0.0 52.9365 1 GTATCAA 4545 0.0 39.187534 1 CAACGCA 5660 0.0 31.189243 5 ATCAACG 5675 0.0 30.871426 3 TCAACGC 5720 0.0 30.79199 4 AACGCAG 6105 0.0 29.07068 6 TATCAAC 6045 0.0 29.062225 2 ACGCAGA 6930 0.0 25.199032 7 GTACATG 6270 0.0 25.149025 1 CGCAGAG 7210 0.0 24.154612 8 TACATGG 6515 0.0 23.758915 2 GTGGTAT 1155 0.0 23.439236 1 ACATGGG 6420 0.0 23.221529 3 CATGGGG 4415 0.0 20.860796 4 GCAGAGT 7955 0.0 20.75909 9 AGAGTAC 7315 0.0 18.71547 10-11 CAGAGTA 7795 0.0 17.928272 10-11 GAGTACT 4780 0.0 17.869558 12-13 GAGTACA 4880 0.0 17.71341 1 AGTACTT 5535 0.0 16.675207 12-13 >>END_MODULE