FastQCFastQC Report
Sat 18 Jun 2016
SRR1930950_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930950_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2339097
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41910.1791717060044966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.050.359461
GTATCAA34050.044.8296971
ATCAACG40300.035.2387733
TCAACGC39950.035.027254
TATCAAC44000.033.1953932
CAACGCA41500.033.1380775
AACGCAG45100.030.2253996
GTACATG45200.025.8837261
ACGCAGA53200.025.3835567
TACATGG46100.024.6062282
CGCAGAG55850.023.8771558
ACATGGG47800.022.5709553
GTGGTAT9150.022.499461
TAACGCA2808.0035534E-1021.4952184
GCAGAGT60050.020.6199179
AGAGTAC53850.019.42642210-11
TGGTATC9850.018.853982
CATGGGG33300.018.827144
CGTATAC2401.829776E-618.8178793
TAGTACT6600.018.2383654
Produced by FastQC (version 0.11.5)