##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930950_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2339097 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.94477441508411 30.0 16.0 31.0 2.0 31.0 2 22.513694387193006 30.0 10.0 31.0 2.0 33.0 3 23.148611194832878 30.0 16.0 31.0 2.0 33.0 4 24.90051331774612 33.0 10.0 35.0 2.0 37.0 5 24.795862676921907 33.0 10.0 35.0 2.0 37.0 6 25.169779192568758 32.0 17.0 35.0 2.0 37.0 7 24.664166984096855 32.0 11.0 35.0 2.0 37.0 8 24.469884746122116 30.0 11.0 35.0 2.0 37.0 9 22.937925618304842 27.0 10.0 34.0 2.0 37.0 10-11 22.87027151930852 27.5 10.0 34.0 2.0 37.0 12-13 22.54857793413441 27.0 10.0 33.5 2.0 37.0 14-15 24.607162935098458 29.5 14.0 35.0 2.0 37.5 16-17 26.56803266388696 32.5 17.0 37.0 2.0 38.5 18-19 27.531102814462162 34.0 18.0 38.0 2.0 39.5 20-21 27.725526132520372 34.5 18.0 38.5 2.0 40.0 22-23 28.12024533399 35.0 18.5 39.0 2.0 40.0 24-25 28.088774856280008 35.0 18.0 39.0 2.0 40.0 26-27 27.93201436280753 35.0 17.5 39.0 2.0 40.0 28-29 28.0692282107155 35.0 18.5 39.0 2.0 40.0 30-31 28.162094175658382 35.0 19.0 39.0 2.0 40.0 32-33 28.034816427022907 35.0 19.0 39.0 2.0 40.0 34-35 24.679685365762943 32.0 8.5 38.0 2.0 40.0 36-37 22.954166501004448 26.0 8.5 37.5 2.0 40.0 38-39 21.931532339189012 26.5 2.0 37.0 2.0 40.0 40-41 23.77758233198538 30.0 5.5 37.5 2.0 40.0 42-43 23.792027222470892 30.5 5.5 38.0 2.0 40.0 44-45 21.476300683554378 23.5 2.0 37.0 2.0 39.5 46-47 22.79404082002585 27.0 7.5 36.5 2.0 39.0 48-49 22.229940442829005 29.0 2.0 37.0 2.0 39.0 50-51 19.600822026619674 16.0 4.5 35.5 2.0 38.5 52-53 23.317723463370694 28.5 7.0 36.5 2.0 39.0 54-55 25.4944649580586 33.0 7.0 38.0 2.0 39.5 56-57 25.84146724141838 33.0 4.0 38.0 2.0 40.0 58-59 25.473260407755642 33.0 2.0 38.0 2.0 40.0 60-61 25.242477332064468 33.0 2.0 38.0 2.0 40.0 62-63 24.82130646997538 32.0 2.0 37.5 2.0 40.0 64-65 24.348322664686414 31.5 2.0 37.0 2.0 39.0 66-67 23.964454231697104 31.0 2.0 36.0 2.0 39.0 68-69 23.534728786364994 31.0 2.0 36.0 2.0 39.0 70-71 22.947683016138278 30.0 2.0 35.0 2.0 38.0 72-73 22.543235060367312 30.0 2.0 35.0 2.0 37.5 74-75 22.022869936560987 29.0 2.0 34.5 2.0 37.0 76-77 21.49625325499541 29.0 2.0 34.0 2.0 36.5 78-79 21.04935814974753 28.5 2.0 34.0 2.0 36.0 80-81 20.595431912400382 27.5 2.0 34.0 2.0 35.5 82-83 20.17239537308628 26.5 2.0 34.0 2.0 35.0 84-85 19.695598985420443 26.0 2.0 34.0 2.0 35.0 86-87 19.156220969032066 25.5 2.0 33.0 2.0 35.0 88-89 18.62457499624855 25.0 2.0 33.0 2.0 35.0 90-91 18.066755675373873 23.5 2.0 33.0 2.0 35.0 92-93 17.440092480132293 20.5 2.0 32.0 2.0 34.0 94-95 16.775858162359235 18.5 2.0 32.0 2.0 34.0 96-97 15.99606087306341 4.5 2.0 32.0 2.0 34.0 98-99 15.08126789953559 2.0 2.0 31.0 2.0 34.0 100-101 13.627280527485606 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.916480580023247 1101 2 8.615029232871247 1101 3 8.058119641877163 1101 4 10.399036690480283 1101 5 10.155008721561753 1101 6 9.69597391685679 1101 7 9.986395822910723 1101 8 9.991904626354778 1101 9 11.685925011031312 1101 10-11 10.643334618617942 1101 12-13 10.125072680968994 1101 14-15 9.044759238300554 1101 16-17 8.07319036402906 1101 18-19 7.860510637746234 1101 20-21 8.443303713730714 1101 22-23 8.25734603144554 1101 24-25 8.457946085799549 1101 26-27 8.485855732872668 1101 28-29 8.40446184093516 1101 30-31 8.26652718474234 1101 32-33 8.23600160322825 1101 34-35 11.236429992261002 1101 36-37 4.46800642790409 1101 38-39 8.280823891589808 1101 40-41 10.192517948505273 1101 42-43 10.657146368339124 1101 44-45 3.731556126066053 1101 46-47 5.387694621201845 1101 48-49 9.522351697609231 1101 50-51 -6.112717137253943 1101 52-53 5.230460834475956 1101 54-55 7.976721347602062 1101 56-57 8.566384588241942 1101 58-59 8.771869975871738 1101 60-61 8.587086358061395 1101 62-63 8.65538980776153 1101 64-65 8.681363462464422 1101 66-67 8.68614016947695 1101 68-69 8.779578729758754 1101 70-71 8.870024007893054 1101 72-73 8.766455893037584 1101 74-75 9.03510465767475 1101 76-77 8.745848577024745 1101 78-79 8.814991962945825 1101 80-81 8.657154596124176 1101 82-83 8.565824038346847 1101 84-85 8.592850084308811 1101 86-87 8.558711920073243 1101 88-89 8.49540148459009 1101 90-91 8.499468142267986 1101 92-93 8.83974027423994 1101 94-95 8.645767078113526 1101 96-97 8.539914399103973 1101 98-99 8.45577668514845 1101 100-101 8.108636923026042 1102 1 7.660577775385239 1102 2 8.190879663637748 1102 3 7.887130984774171 1102 4 10.269014543323461 1102 5 10.13051505484638 1102 6 9.23839016307129 1102 7 9.634763150553987 1102 8 9.167726692716027 1102 9 9.811405988256706 1102 10-11 9.57006993287549 1102 12-13 8.632890799234236 1102 14-15 7.759643961346487 1102 16-17 7.226129661336728 1102 18-19 7.357138425122063 1102 20-21 7.943611711060456 1102 22-23 7.808935584176115 1102 24-25 8.052547823764016 1102 26-27 8.218928772648727 1102 28-29 8.089690718764796 1102 30-31 7.938756426438584 1102 32-33 7.824580918987113 1102 34-35 10.804031108500624 1102 36-37 0.03312924004864115 1102 38-39 2.5956071903987876 1102 40-41 8.629725171973814 1102 42-43 9.887791959393166 1102 44-45 0.3489238806079378 1102 46-47 0.4680554221668096 1102 48-49 8.135736258777502 1102 50-51 -7.186226788314457 1102 52-53 4.191312941320945 1102 54-55 7.378032556699299 1102 56-57 7.938088658677348 1102 58-59 8.333397814532702 1102 60-61 8.239898875072878 1102 62-63 8.278937564538182 1102 64-65 8.289168258054477 1102 66-67 8.178784047206307 1102 68-69 8.046214166928735 1102 70-71 8.06690234751526 1102 72-73 8.191116164820162 1102 74-75 8.091081381671895 1102 76-77 7.967182620900758 1102 78-79 8.016729497649244 1102 80-81 7.819416900975636 1102 82-83 7.692226858742863 1102 84-85 7.757740394283054 1102 86-87 7.561605662427031 1102 88-89 7.497369802408951 1102 90-91 7.715065556809474 1102 92-93 7.690264156209629 1102 94-95 7.577825732265477 1102 96-97 7.6925170481675185 1102 98-99 7.833905006863837 1102 100-101 7.271003803715038 1103 1 7.657375863796229 1103 2 8.074067093491845 1103 3 7.712688350546589 1103 4 9.793564704432757 1103 5 9.644508031204946 1103 6 8.7339449945401 1103 7 9.03603175053226 1103 8 8.60545306033487 1103 9 9.032087353884304 1103 10-11 7.899052953041309 1103 12-13 6.975014545659061 1103 14-15 6.807495958015689 1103 16-17 7.217799767415443 1103 18-19 7.414612810553447 1103 20-21 7.958325877817629 1103 22-23 8.073659526104354 1103 24-25 8.373649159704058 1103 26-27 8.382324739485558 1103 28-29 8.3744298665147 1103 30-31 8.18798056749068 1103 32-33 8.188822619142787 1103 34-35 10.957343443318337 1103 36-37 -4.077476458514045 1103 38-39 -2.4277670538356695 1103 40-41 7.532727235621035 1103 42-43 9.921407686557277 1103 44-45 0.09352327961594042 1103 46-47 -0.580732818664444 1103 48-49 8.337452344364554 1103 50-51 -7.399283520510975 1103 52-53 4.050622886652398 1103 54-55 7.334265857448724 1103 56-57 7.931820239720761 1103 58-59 8.23692484107049 1103 60-61 8.163394765880593 1103 62-63 8.026628089863124 1103 64-65 8.17324486643216 1103 66-67 8.057822460008161 1103 68-69 8.093491962808677 1103 70-71 8.155646419055035 1103 72-73 7.946469700173697 1103 74-75 8.02533792560586 1103 76-77 8.084309444694249 1103 78-79 7.941986367422242 1103 80-81 8.082883104226784 1103 82-83 7.930998807622338 1103 84-85 7.870175860051852 1103 86-87 7.731116541830385 1103 88-89 7.844764176254937 1103 90-91 7.842459930655458 1103 92-93 7.9538831417640985 1103 94-95 7.794374010749237 1103 96-97 8.07524540702491 1103 98-99 7.663881474044608 1103 100-101 7.4100067362663555 1104 1 7.221014122852782 1104 2 7.813091267996864 1104 3 7.2522929974850925 1104 4 9.439250649102288 1104 5 8.924133843668915 1104 6 8.26970351800777 1104 7 8.337964763223681 1104 8 7.569803839431543 1104 9 7.447267774193733 1104 10-11 6.558429438774322 1104 12-13 5.97245138472999 1104 14-15 6.329301424723422 1104 16-17 6.588663052336585 1104 18-19 7.312287761785857 1104 20-21 7.889781402572609 1104 22-23 7.989876504330713 1104 24-25 8.093599720827413 1104 26-27 8.263993972867066 1104 28-29 8.008455581048626 1104 30-31 7.745902871058018 1104 32-33 7.748236381472889 1104 34-35 10.643249493657535 1104 36-37 -4.327784148391711 1104 38-39 -2.5952264799972085 1104 40-41 6.927090571876477 1104 42-43 9.266235953590392 1104 44-45 -0.21591654474178767 1104 46-47 -1.3591555921380376 1104 48-49 7.722993842220358 1104 50-51 -7.694231909798692 1104 52-53 3.6177720957093484 1104 54-55 6.665943699196934 1104 56-57 7.191136545862708 1104 58-59 7.485656725986885 1104 60-61 7.252835853851781 1104 62-63 7.472338438067606 1104 64-65 7.691704837527002 1104 66-67 7.53374730659759 1104 68-69 7.432941106315681 1104 70-71 7.545475515334481 1104 72-73 7.340560174897476 1104 74-75 7.480166903421544 1104 76-77 7.42229319146611 1104 78-79 7.327633140074489 1104 80-81 7.234088670444077 1104 82-83 7.221034662654283 1104 84-85 7.03217837148361 1104 86-87 7.061379215478517 1104 88-89 7.061676220860495 1104 90-91 7.122087638539615 1104 92-93 7.1411374676658035 1104 94-95 7.049440074040479 1104 96-97 6.97229968712063 1104 98-99 6.670892317427615 1104 100-101 6.3805673067878335 1105 1 6.52419794311027 1105 2 7.333212242733456 1105 3 7.0674950997102535 1105 4 9.019427837369843 1105 5 8.484548305328818 1105 6 7.630097678940377 1105 7 7.39994954584877 1105 8 6.58703463167528 1105 9 5.852673927011072 1105 10-11 5.473789547279509 1105 12-13 4.774731571220137 1105 14-15 5.146712723743708 1105 16-17 6.089962958814965 1105 18-19 6.918926445293128 1105 20-21 7.70952257144198 1105 22-23 7.721794087148883 1105 24-25 7.825058023259217 1105 26-27 7.937827435253528 1105 28-29 7.688624221641522 1105 30-31 7.552274471157585 1105 32-33 7.317663926103069 1105 34-35 10.11036042990446 1105 36-37 -4.57825238498129 1105 38-39 -2.7675144473853166 1105 40-41 6.577763449853393 1105 42-43 8.911562449622007 1105 44-45 -3.9644712963689877 1105 46-47 -5.278458629830766 1105 48-49 5.993639619040575 1105 50-51 -12.130551262236915 1105 52-53 0.6815016095729192 1105 54-55 5.809465441715833 1105 56-57 6.779345248652522 1105 58-59 7.21732149247196 1105 60-61 7.09251368822299 1105 62-63 7.001777006684065 1105 64-65 6.875751199862929 1105 66-67 6.651757225671226 1105 68-69 6.730060948485381 1105 70-71 6.75669955491524 1105 72-73 6.74338929808204 1105 74-75 6.696130660024 1105 76-77 6.44416596812794 1105 78-79 6.405542994789162 1105 80-81 6.334777449609877 1105 82-83 6.222774522975222 1105 84-85 6.292829811138283 1105 86-87 6.084603907447974 1105 88-89 6.101457475809976 1105 90-91 6.07551711016762 1105 92-93 6.068289910415242 1105 94-95 6.075671064891953 1105 96-97 5.984243019808696 1105 98-99 5.824871762784092 1105 100-101 5.330843723502058 1106 1 6.468864005573661 1106 2 7.372121760569183 1106 3 7.007179627664922 1106 4 8.608914448945914 1106 5 7.887777315130993 1106 6 7.03201782606094 1106 7 7.02600229254346 1106 8 5.995917991112542 1106 9 5.079011323841893 1106 10-11 4.354559227671899 1106 12-13 3.9673692893429653 1106 14-15 4.7329370775772155 1106 16-17 5.978811605894052 1106 18-19 6.781990654670924 1106 20-21 7.504519479194968 1106 22-23 7.187296363595767 1106 24-25 7.4649836144943365 1106 26-27 7.44949045449334 1106 28-29 7.373193657670985 1106 30-31 7.050633129072093 1106 32-33 7.121574927211743 1106 34-35 10.06916818013135 1106 36-37 -0.6819671038256452 1106 38-39 0.632881416797165 1106 40-41 7.405589399085233 1106 42-43 8.738479894168972 1106 44-45 -4.097073698768373 1106 46-47 -5.348209540535223 1106 48-49 5.938005570132791 1106 50-51 -12.015967640838566 1106 52-53 0.4875922677187283 1106 54-55 5.693895223825553 1106 56-57 6.583239751492105 1106 58-59 7.000903012147951 1106 60-61 6.843336244782197 1106 62-63 7.028046022117643 1106 64-65 6.841040255822545 1106 66-67 6.647703522391179 1106 68-69 6.459133444079555 1106 70-71 6.636171371472088 1106 72-73 6.471912794131253 1106 74-75 6.481926090286283 1106 76-77 6.421511330637337 1106 78-79 6.260393108800056 1106 80-81 5.837294299842046 1106 82-83 6.036320041856783 1106 84-85 5.838276885948034 1106 86-87 5.81782956731589 1106 88-89 5.936383023049704 1106 90-91 5.792337834004822 1106 92-93 5.744929492685355 1106 94-95 5.7445143508192125 1106 96-97 5.736764112452919 1106 98-99 5.374957993737233 1106 100-101 5.189597799360159 1107 1 5.818552334384464 1107 2 6.905273272933318 1107 3 6.417513068312484 1107 4 7.756975280784513 1107 5 7.338399890979158 1107 6 6.057296153185376 1107 7 6.316305071982054 1107 8 5.238548722842296 1107 9 4.143352951032256 1107 10-11 3.5926833749070255 1107 12-13 3.0760008795865694 1107 14-15 4.374067556351992 1107 16-17 5.976326208294822 1107 18-19 6.796133903839237 1107 20-21 7.471653269380319 1107 22-23 7.357759347851239 1107 24-25 7.5809159452061365 1107 26-27 7.816436444773732 1107 28-29 7.5876324808937845 1107 30-31 7.264367970936913 1107 32-33 7.003356190146949 1107 34-35 9.89857933161305 1107 36-37 -0.7433825785378119 1107 38-39 0.490396939034202 1107 40-41 7.644575126292455 1107 42-43 8.896743396598875 1107 44-45 -4.0517946051137095 1107 46-47 -5.203602693887259 1107 48-49 5.952265178589151 1107 50-51 -12.006223508627434 1107 52-53 0.6246038729863272 1107 54-55 5.676143456973385 1107 56-57 6.787375795276315 1107 58-59 7.217679445942736 1107 60-61 7.170863571584693 1107 62-63 6.946455778514338 1107 64-65 6.89975110529134 1107 66-67 6.917965062503704 1107 68-69 6.599623870117057 1107 70-71 6.735224553339048 1107 72-73 6.504122700767869 1107 74-75 6.590388430656365 1107 76-77 6.666412831503514 1107 78-79 6.66832623670329 1107 80-81 6.425265754256493 1107 82-83 6.342472366742953 1107 84-85 6.229534148821909 1107 86-87 5.945739239394257 1107 88-89 6.060509868471218 1107 90-91 5.86369581895017 1107 92-93 5.7034850431307085 1107 94-95 5.54705969108198 1107 96-97 5.3665110041728585 1107 98-99 5.447238574460528 1107 100-101 5.272395328066716 1108 1 5.649257584226337 1108 2 6.144190097727751 1108 3 6.517024453511727 1108 4 7.4286372036181625 1108 5 6.948202231270113 1108 6 6.632212345468741 1108 7 6.118604249717663 1108 8 5.058581929673416 1108 9 2.1358434633662213 1108 10-11 3.0313179416369422 1108 12-13 3.2261305443557013 1108 14-15 4.357879226181215 1108 16-17 5.495628864841315 1108 18-19 6.668789659689967 1108 20-21 7.651389986647871 1108 22-23 7.780647551519795 1108 24-25 7.526270942043581 1108 26-27 7.774165730036227 1108 28-29 7.802673594113262 1108 30-31 7.596240994339819 1108 32-33 7.598958656940116 1108 34-35 10.384283000177248 1108 36-37 -4.389655507064063 1108 38-39 -2.6735783930341057 1108 40-41 6.5593324001697475 1108 42-43 8.82706913979938 1108 44-45 -4.023938026998589 1108 46-47 -5.202327393444502 1108 48-49 6.197392471441777 1108 50-51 -12.143885589588852 1108 52-53 0.8971995402855022 1108 54-55 5.970214456340873 1108 56-57 6.8945128920505105 1108 58-59 7.39373732070554 1108 60-61 7.167589536164076 1108 62-63 7.09320229970346 1108 64-65 7.198731181192667 1108 66-67 6.9201843857168654 1108 68-69 6.9155117575300835 1108 70-71 6.897445457829878 1108 72-73 6.7727469891929175 1108 74-75 6.71540187151658 1108 76-77 6.572262768252408 1108 78-79 6.493200525128337 1108 80-81 6.545385140720757 1108 82-83 6.405911645680334 1108 84-85 6.636314667480985 1108 86-87 6.397024818141887 1108 88-89 6.294741367522452 1108 90-91 6.454439810095014 1108 92-93 6.639017143067456 1108 94-95 6.2787516581914 1108 96-97 6.289014957367039 1108 98-99 6.26395030754086 1108 100-101 5.78478068543547 1109 1 4.303311382479624 1109 2 6.041722277464306 1109 3 5.83571432048474 1109 4 6.899196497883249 1109 5 5.999858378056707 1109 6 4.650188448913866 1109 7 3.50919086085851 1109 8 2.2492378936947723 1109 9 0.8391888831388101 1109 10-11 0.572782723428304 1109 12-13 1.270759530922458 1109 14-15 2.686184584466357 1109 16-17 2.522246174014821 1109 18-19 4.055573203638485 1109 20-21 4.649609086267979 1109 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2313 92-93 -14.502453403767849 2313 94-95 -14.25431285793763 2313 96-97 -13.367556558995446 2313 98-99 -12.26992091768882 2313 100-101 -10.824081270235443 2314 1 -20.369290802870438 2314 2 -19.944922805498056 2314 3 -20.53660457874634 2314 4 -22.210475699607645 2314 5 -22.123168736471822 2314 6 -22.52544894485384 2314 7 -22.008008653508142 2314 8 -21.84262774774594 2314 9 -20.304882343085392 2314 10-11 -20.297512703524347 2314 12-13 -19.971450100805587 2314 14-15 -22.0024030318833 2314 16-17 -23.978967909352235 2314 18-19 -24.913670017729384 2314 20-21 -25.081876142384388 2314 22-23 -25.460279867835037 2314 24-25 -25.425946799891904 2314 26-27 -25.264543947383128 2314 28-29 -25.416681244596415 2314 30-31 -25.5148477153376 2314 32-33 -25.383702633382466 2314 34-35 -22.18063635466146 2314 36-37 -20.418284539322126 2314 38-39 -19.450191296259913 2314 40-41 -21.271111148217347 2314 42-43 -21.270511505361913 2314 44-45 -18.96728480119214 2314 46-47 -20.191641909573534 2314 48-49 -19.732244625332417 2314 50-51 -17.121909783137237 2314 52-53 -20.7473569113274 2314 54-55 -22.835229092046223 2314 56-57 -23.172624204723686 2314 58-59 -22.784085259939616 2314 60-61 -22.555999173513346 2314 62-63 -22.1525638293288 2314 64-65 -21.68554301235572 2314 66-67 -21.310662388476686 2314 68-69 -20.88468985537314 2314 70-71 -20.312030348621736 2314 72-73 -19.919994946290952 2314 74-75 -19.402944902676968 2314 76-77 -18.890842070457268 2314 78-79 -18.455791410355534 2314 80-81 -18.016106794763115 2314 82-83 -17.612233515609987 2314 84-85 -17.12497565509966 2314 86-87 -16.578378407664566 2314 88-89 -16.06654895505819 2314 90-91 -15.51551986732434 2314 92-93 -14.919652211771254 2314 94-95 -14.25431285793763 2314 96-97 -13.49505762327812 2314 98-99 -12.60197711181398 2314 100-101 -11.202114475854854 2315 1 -20.369290802870438 2315 2 -19.944922805498056 2315 3 -20.53660457874634 2315 4 -21.765366771799016 2315 5 -22.123168736471822 2315 6 -22.52544894485384 2315 7 -22.008008653508142 2315 8 -21.84262774774594 2315 9 -19.903771706605255 2315 10-11 -19.77850373299893 2315 12-13 -19.137831988887605 2315 14-15 -21.11538893534336 2315 16-17 -23.557566798715754 2315 18-19 -24.913670017729384 2315 20-21 -25.081876142384388 2315 22-23 -25.460279867835037 2315 24-25 -25.425946799891904 2315 26-27 -25.264543947383128 2315 28-29 -25.416681244596415 2315 30-31 -25.5148477153376 2315 32-33 -25.383702633382466 2315 34-35 -22.18063635466146 2315 36-37 -20.418284539322126 2315 38-39 -19.450191296259913 2315 40-41 -21.271111148217347 2315 42-43 -21.270511505361913 2315 44-45 -18.96728480119214 2315 46-47 -20.191641909573534 2315 48-49 -19.732244625332417 2315 50-51 -17.121909783137237 2315 52-53 -20.7473569113274 2315 54-55 -22.835229092046223 2315 56-57 -23.172624204723686 2315 58-59 -22.784085259939616 2315 60-61 -22.555999173513346 2315 62-63 -22.1525638293288 2315 64-65 -21.68554301235572 2315 66-67 -21.310662388476686 2315 68-69 -20.88468985537314 2315 70-71 -20.312030348621736 2315 72-73 -19.919994946290952 2315 74-75 -19.402944902676968 2315 76-77 -18.890842070457268 2315 78-79 -18.455791410355534 2315 80-81 -18.016106794763115 2315 82-83 -17.612233515609987 2315 84-85 -17.12497565509966 2315 86-87 -16.578378407664566 2315 88-89 -16.06654895505819 2315 90-91 -15.51551986732434 2315 92-93 -14.919652211771254 2315 94-95 -14.25431285793763 2315 96-97 -13.49505762327812 2315 98-99 -12.60197711181398 2315 100-101 -11.202114475854854 2316 1 -20.369290802870438 2316 2 -19.944922805498056 2316 3 -20.53660457874634 2316 4 -22.210475699607645 2316 5 -22.123168736471822 2316 6 -22.52544894485384 2316 7 -22.008008653508142 2316 8 -21.84262774774594 2316 9 -20.304882343085392 2316 10-11 -20.297512703524347 2316 12-13 -19.971450100805587 2316 14-15 -22.0024030318833 2316 16-17 -23.978967909352235 2316 18-19 -24.913670017729384 2316 20-21 -25.081876142384388 2316 22-23 -25.460279867835037 2316 24-25 -25.425946799891904 2316 26-27 -25.264543947383128 2316 28-29 -25.416681244596415 2316 30-31 -25.5148477153376 2316 32-33 -25.383702633382466 2316 34-35 -22.18063635466146 2316 36-37 -20.418284539322126 2316 38-39 -19.450191296259913 2316 40-41 -21.271111148217347 2316 42-43 -21.270511505361913 2316 44-45 -18.96728480119214 2316 46-47 -20.191641909573534 2316 48-49 -19.732244625332417 2316 50-51 -17.121909783137237 2316 52-53 -20.7473569113274 2316 54-55 -22.835229092046223 2316 56-57 -23.172624204723686 2316 58-59 -22.784085259939616 2316 60-61 -22.555999173513346 2316 62-63 -22.1525638293288 2316 64-65 -21.68554301235572 2316 66-67 -21.310662388476686 2316 68-69 -20.88468985537314 2316 70-71 -20.312030348621736 2316 72-73 -19.919994946290952 2316 74-75 -19.402944902676968 2316 76-77 -18.890842070457268 2316 78-79 -18.455791410355534 2316 80-81 -18.016106794763115 2316 82-83 -17.612233515609987 2316 84-85 -17.12497565509966 2316 86-87 -16.578378407664566 2316 88-89 -16.06654895505819 2316 90-91 -15.51551986732434 2316 92-93 -14.919652211771254 2316 94-95 -14.25431285793763 2316 96-97 -13.49505762327812 2316 98-99 -12.60197711181398 2316 100-101 -11.202114475854854 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 368985.0 3 8643.0 4 9869.0 5 6924.0 6 8941.0 7 14061.0 8 21170.0 9 25332.0 10 26070.0 11 25162.0 12 25183.0 13 24774.0 14 26378.0 15 29836.0 16 33352.0 17 34821.0 18 36737.0 19 39506.0 20 42503.0 21 46771.0 22 50512.0 23 56182.0 24 62615.0 25 69104.0 26 76269.0 27 86016.0 28 95860.0 29 107091.0 30 115869.0 31 120706.0 32 122844.0 33 126003.0 34 126724.0 35 117389.0 36 94940.0 37 48344.0 38 7542.0 39 69.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.41131440522087 19.93821146599944 14.434934530742474 24.21553959803721 2 14.937144110512826 20.435905486074077 40.47355880885616 24.153391594556933 3 18.08590783236435 25.793648158807773 30.3430889085132 25.777355100314676 4 12.041196545479872 16.68190207549798 39.92868740423457 31.348213974787576 5 12.721403080518156 37.64972177173501 36.36942111472647 13.259454033020365 6 30.023018096463993 38.42300800080765 18.009590873526665 13.544383029201686 7 25.643905398176937 34.55578322025913 22.53810838190162 17.26220299966232 8 24.202975327190458 38.25906594758584 20.335455258640405 17.202503466583295 9 24.840011597921702 20.150289002081553 19.92933011442608 35.08036928557066 10-11 23.30727879795104 30.470644014007693 27.441610711356223 18.78046647668505 12-13 23.726892324052553 27.909071747175968 27.628332602345484 20.735703326426 14-15 21.709102507361415 27.428462047187494 25.858218747141954 25.00421669830914 16-17 21.734573719263157 29.426357481527315 26.95338000369334 21.88568879551619 18-19 21.804526943933343 28.206684502457342 28.84080093382419 21.147987619785127 20-21 22.4574373736752 27.313044282176115 28.780062841252946 21.449455502895738 22-23 22.290721875342314 27.0108445612882 28.703910614525142 21.99452294884434 24-25 22.132270335381662 27.108325590443833 28.723328343332117 22.036075730842388 26-27 22.073031718343625 27.276937096830405 28.538051946437655 22.11197923838831 28-29 22.1141195149025 27.43786104805526 28.548107045074882 21.899912391967355 30-31 22.34417625703372 27.183401093922694 28.466996064681343 22.00542658436224 32-33 22.181331646113346 27.348757271540837 28.32210132413876 22.147809758207057 34-35 22.544568849991443 27.33786777704711 28.24493125078465 21.872632122176793 36-37 22.95859330244881 27.28966232065293 27.889183737512518 21.86256063938574 38-39 22.673818664206678 27.348309133860262 27.989025711676714 21.988846490256346 40-41 22.73945871825749 27.20914624782644 28.13092186869034 21.920473165225726 42-43 22.767317067250524 27.31333852703544 27.788440905542704 22.130903500171332 44-45 23.052401278003227 27.39939477237189 27.590532356941367 21.957671592683518 46-47 22.440599884830124 27.37627806840332 27.83000556889373 22.353116477872824 48-49 22.891640390679076 27.491712438829907 27.37408776112395 22.242559409367068 50-51 23.327546610149014 27.48582539202678 27.19921881921851 21.9874091786057 52-53 22.354611352897734 27.560305248641892 27.69956956404318 22.38551383441719 54-55 22.091927450093912 27.451358541472466 27.876201429372937 22.580512579060684 56-57 22.205289752057837 27.608561927727866 27.68993454148927 22.49621377872503 58-59 22.238593325645464 27.513940172600304 27.798492401584607 22.448974100169625 60-61 22.223278050591947 27.53242864773985 27.84707900355941 22.397214298108793 62-63 22.170445996462654 27.48336206626412 27.82108837604973 22.525103561223492 64-65 22.04213739227171 27.395818508902526 27.73299826740041 22.82904583142536 66-67 22.113174169339988 27.465705235850695 27.709850950235676 22.71126964457364 68-69 22.126345979708766 27.472101932959724 27.75520024166572 22.64635184566579 70-71 22.0425893108332 27.30433611699557 27.7073139062083 22.945760665962936 72-73 22.10959633524793 27.396505018499262 27.668925896697832 22.824972749554977 74-75 22.063197434740374 27.559475552020313 27.550703840241077 22.826623172998232 76-77 22.097366454428602 27.379882222820072 27.56521687143156 22.95753445131976 78-79 22.03883702661415 27.42149031451906 27.49308162778617 23.04659103108062 80-81 22.12128640309925 27.414610769932196 27.46402095683379 23.000081870134757 82-83 22.216232695098146 27.372301758731027 27.551601574870137 22.859863971300687 84-85 22.188873693983737 27.340985238909 27.411711187896138 23.058429879211126 86-87 22.032718865461 27.444988960196646 27.401387766412988 23.120904407929363 88-89 22.096500968186977 27.285450413924227 27.284010552266135 23.334038065622657 90-91 22.046517627791676 27.316603850482522 27.417394848325088 23.219483673400713 92-93 22.14945061976432 27.122439414895304 27.4463064711097 23.281803494230676 94-95 21.89867154896721 27.069137773613587 27.564617980697065 23.467572696722133 96-97 22.007986995855678 26.79074018521818 27.539292075355714 23.661980743570428 98-99 21.770459961033733 26.97619638428194 27.394211099330732 23.859132555353586 100-101 21.985155162627137 26.9239064755746 27.312969340818928 23.777969020979338 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 30758.0 1 29361.5 2 19964.5 3 13808.5 4 18334.0 5 22225.5 6 22973.5 7 21290.0 8 18965.0 9 16789.0 10 14559.5 11 12429.5 12 10617.5 13 9013.5 14 7738.5 15 6841.5 16 6175.5 17 5848.0 18 5937.0 19 6228.5 20 6707.0 21 7498.0 22 8582.0 23 9914.5 24 11769.0 25 14339.0 26 17406.0 27 21016.5 28 25205.5 29 30114.0 30 35199.0 31 40570.0 32 45993.0 33 49902.0 34 53180.5 35 56267.5 36 59672.5 37 63248.5 38 65038.0 39 66059.0 40 67435.5 41 68656.5 42 70031.0 43 71393.5 44 73075.0 45 74860.5 46 75575.0 47 75557.5 48 74613.5 49 72860.0 50 70719.5 51 68182.0 52 65628.5 53 62537.5 54 58482.0 55 53520.5 56 47804.5 57 41958.0 58 36171.5 59 30422.0 60 24977.5 61 20258.0 62 16118.0 63 12186.5 64 8968.5 65 6604.0 66 4828.5 67 3643.0 68 2781.5 69 2053.0 70 1522.0 71 1121.5 72 759.5 73 523.0 74 374.0 75 282.0 76 187.0 77 112.5 78 66.0 79 31.5 80 21.5 81 12.5 82 4.5 83 2.5 84 3.5 85 3.0 86 1.5 87 1.0 88 1.0 89 0.5 90 0.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.81682760484067 2 20.476662575344246 3 16.297229229912226 4 21.430192933426873 5 21.258802007783345 6 15.307060801668337 7 14.162345554716198 8 11.391190703079008 9 2.8334010945249384 10-11 6.132537470656412 12-13 3.354178984454257 14-15 6.978077437575269 16-17 10.870092176596353 18-19 13.821573025830054 20-21 14.21969674622301 22-23 12.18726713770314 24-25 14.625451616585375 26-27 12.296133935446028 28-29 12.284420013364132 30-31 13.064421868780988 32-33 11.619483929054674 34-35 18.44312142677281 36-37 23.096327343415002 38-39 22.235845713110656 40-41 17.31685346952264 42-43 17.21935858153809 44-45 22.53226351878524 46-47 12.061727239186746 48-49 20.784495042317612 50-51 20.383421465633962 52-53 10.560998539179863 54-55 12.10554756814275 56-57 11.631090972285458 58-59 12.619570714681776 60-61 12.044562495698125 62-63 12.003499641100818 64-65 13.033683511201117 66-67 11.40478569294048 68-69 12.397326831679061 70-71 11.102660556616506 72-73 10.478167429567906 74-75 10.565829463250134 76-77 10.614544843587076 78-79 10.993152485766943 80-81 10.967074046095567 82-83 9.549283334551752 84-85 9.450762409596523 86-87 9.596694792905126 88-89 10.925733306485366 90-91 8.529830101103117 92-93 10.468719339129587 94-95 10.56762502794882 96-97 10.145175681042726 98-99 9.979470710278369 100-101 10.1099056601757 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2339097.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.18006649517089 #Duplication Level Percentage of deduplicated Percentage of total 1 98.97616779502856 96.18510567758068 2 0.7171452744362696 1.3938445091282852 3 0.1412190720382875 0.4117103643320133 4 0.05139145817475636 0.19976901290826468 5 0.028667889742858815 0.13929737157436237 6 0.01797068487585593 0.10478354107196647 7 0.013909746404368506 0.0946225056355247 8 0.006509923356956493 0.050610782776599854 9 0.007384791130838139 0.06458890438330189 >10 0.035665305597197355 0.6205894504857435 >50 0.0024654764176503976 0.16875187844034087 >100 0.0013261999194373586 0.23284979759979524 >500 4.409571926569316E-5 0.03333904995845326 >1k 1.322871577970793E-4 0.30013715412468683 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4191 0.1791717060044966 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2825462133464325E-4 2 0.0 0.0 0.0 0.0 1.2825462133464325E-4 3 0.0 0.0 0.0 0.0 1.2825462133464325E-4 4 0.0 4.275154044488108E-5 0.0 0.0 1.2825462133464325E-4 5 0.0 4.275154044488108E-5 0.0 0.0 1.2825462133464325E-4 6 0.0 4.275154044488108E-5 0.0 0.0 1.2825462133464325E-4 7 0.0 4.275154044488108E-5 0.0 0.0 1.2825462133464325E-4 8 0.0 4.275154044488108E-5 0.0 0.0 1.2825462133464325E-4 9 0.0 4.275154044488108E-5 0.0 0.0 1.2825462133464325E-4 10-11 0.0 4.275154044488108E-5 0.0 0.0 1.2825462133464325E-4 12-13 0.0 4.275154044488108E-5 0.0 0.0 1.7100616177952432E-4 14-15 0.0 4.275154044488108E-5 0.0 0.0 2.137577022244054E-4 16-17 0.0 4.275154044488108E-5 0.0 0.0 2.565092426692865E-4 18-19 0.0 4.275154044488108E-5 0.0 0.0 2.565092426692865E-4 20-21 0.0 4.275154044488108E-5 0.0 4.275154044488108E-5 3.4201232355904863E-4 22-23 0.0 4.275154044488108E-5 0.0 8.550308088976216E-5 3.4201232355904863E-4 24-25 0.0 4.275154044488108E-5 0.0 2.137577022244054E-4 3.4201232355904863E-4 26-27 0.0 4.275154044488108E-5 0.0 4.0613963422637023E-4 3.6338809378148916E-4 28-29 0.0 4.275154044488108E-5 0.0 0.0012397946729015513 3.847638640039297E-4 30-31 0.0 4.275154044488108E-5 0.0 0.004275154044488108 4.0613963422637023E-4 32-33 0.0 4.275154044488108E-5 0.0 0.013381232159247778 4.4889117467125135E-4 34-35 0.0 4.275154044488108E-5 0.0 0.026334948914046745 4.702669448936919E-4 36-37 0.0 4.275154044488108E-5 0.0 0.04557314211424323 4.702669448936919E-4 38-39 0.0 4.275154044488108E-5 0.0 0.07306238262030176 4.916427151161325E-4 40-41 0.0 4.275154044488108E-5 0.0 0.10290295785082876 5.13018485338573E-4 42-43 0.0 4.275154044488108E-5 0.0 0.13325655156669433 5.13018485338573E-4 44-45 0.0 4.275154044488108E-5 0.0 0.1731009872613235 5.343942555610135E-4 46-47 0.0 4.275154044488108E-5 0.0 0.2190802690097931 5.557700257834541E-4 48-49 0.0 4.275154044488108E-5 0.0 0.2671543762400619 5.985215662283351E-4 50-51 0.0 4.275154044488108E-5 0.0 0.3243986888957576 6.840246471180973E-4 52-53 0.0 4.275154044488108E-5 0.0 0.3976107019076165 8.122792684527406E-4 54-55 0.0 4.275154044488108E-5 0.0 0.48215187313736885 8.550308088976216E-4 56-57 0.0 8.550308088976216E-5 0.0 0.5813140711992705 8.977823493425027E-4 58-59 0.0 8.550308088976216E-5 0.0 0.6885349346350322 8.977823493425027E-4 60-61 0.0 8.550308088976216E-5 0.0 0.7985773997401562 8.977823493425027E-4 62-63 0.0 8.550308088976216E-5 0.0 0.9135576677666638 0.001026036970677146 64-65 0.0 8.550308088976216E-5 0.0 1.0356560672772441 0.001068788511122027 66-67 0.0 1.2825462133464325E-4 0.0 1.1776766846351392 0.0011115400515669081 68-69 0.0 1.2825462133464325E-4 0.0 1.3341686984336263 0.0011115400515669081 70-71 0.0 1.4963039155708378E-4 0.0 1.4991468930104226 0.0011115400515669081 72-73 0.0 1.7100616177952432E-4 0.0 1.6691270178192696 0.001218418902679111 74-75 0.0 1.7100616177952432E-4 0.0 1.844557964034839 0.0012825462133464324 76-77 0.0 1.7100616177952432E-4 0.0 2.040295891961727 0.0012825462133464324 78-79 0.0 1.7100616177952432E-4 0.0 2.26489110968891 0.0012825462133464324 80-81 2.137577022244054E-5 1.7100616177952432E-4 0.0 2.507933617118059 0.001303921983568873 82-83 4.275154044488108E-5 1.7100616177952432E-4 0.0 2.7603387119046365 0.0013252977537913135 84-85 4.275154044488108E-5 1.7100616177952432E-4 0.0 3.013898098283226 0.0013252977537913135 86-87 4.275154044488108E-5 1.7100616177952432E-4 0.0 3.293514548562971 0.0014108008346810756 88-89 4.275154044488108E-5 1.7100616177952432E-4 0.0 3.595661060657168 0.0014963039155708377 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1705 0.0 50.35946 1 GTATCAA 3405 0.0 44.829697 1 ATCAACG 4030 0.0 35.238773 3 TCAACGC 3995 0.0 35.02725 4 TATCAAC 4400 0.0 33.195393 2 CAACGCA 4150 0.0 33.138077 5 AACGCAG 4510 0.0 30.225399 6 GTACATG 4520 0.0 25.883726 1 ACGCAGA 5320 0.0 25.383556 7 TACATGG 4610 0.0 24.606228 2 CGCAGAG 5585 0.0 23.877155 8 ACATGGG 4780 0.0 22.570955 3 GTGGTAT 915 0.0 22.49946 1 TAACGCA 280 8.0035534E-10 21.495218 4 GCAGAGT 6005 0.0 20.619917 9 AGAGTAC 5385 0.0 19.426422 10-11 TGGTATC 985 0.0 18.85398 2 CATGGGG 3330 0.0 18.82714 4 CGTATAC 240 1.829776E-6 18.817879 3 TAGTACT 660 0.0 18.238365 4 >>END_MODULE