FastQCFastQC Report
Sat 18 Jun 2016
SRR1930950_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930950_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2339097
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40630.17369950882755184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33920.14501322518903662No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23470.10033786542413589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26250.055.523671
GTATCAA50650.043.304361
TCAACGC63650.034.0071754
ATCAACG64400.033.6111263
CAACGCA64650.033.3984455
TATCAAC67700.032.0428852
AACGCAG68300.031.5434536
ACGCAGA78250.027.3505657
CGCAGAG81300.026.207768
GTACATG66250.025.5829981
ACATGGG65150.024.6267073
TACATGG68800.024.1481382
GCAGAGT91650.022.6785729
CATGGGG45850.022.2588734
GTGGTAT11900.021.5434821
TATACCG1353.8218664E-421.0911775
AGAGTAC85200.020.94220210-11
TATCACG1605.0178183E-520.76742
CAGAGTA90300.018.81348810-11
GAGTACT60300.018.49373412-13
Produced by FastQC (version 0.11.5)