##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930950_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2339097 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.23545282645397 31.0 31.0 33.0 30.0 34.0 2 31.352738257541265 31.0 31.0 34.0 30.0 34.0 3 31.434791716632528 31.0 31.0 34.0 28.0 34.0 4 35.30814412570321 37.0 35.0 37.0 33.0 37.0 5 35.09181705589807 35.0 35.0 37.0 32.0 37.0 6 35.1282794172281 37.0 35.0 37.0 32.0 37.0 7 35.00467787355548 36.0 35.0 37.0 32.0 37.0 8 34.97821595256631 36.0 35.0 37.0 32.0 37.0 9 36.4811651675839 38.0 35.0 39.0 32.0 39.0 10-11 36.40261177710886 38.0 35.0 39.0 32.0 39.0 12-13 36.304110731619936 38.0 35.0 39.0 32.0 39.0 14-15 37.32085180734275 39.0 36.0 41.0 32.0 41.0 16-17 37.287640914421246 39.0 36.0 40.5 32.0 41.0 18-19 37.27505614346049 39.0 36.0 40.0 31.5 41.0 20-21 37.23594126280355 39.0 36.0 40.0 31.0 41.0 22-23 37.109611957092845 39.0 36.0 40.0 31.0 41.0 24-25 36.99217946070642 39.0 36.0 40.0 31.0 41.0 26-27 36.71824960657895 39.0 35.5 40.0 30.0 41.0 28-29 36.52656965487109 38.0 35.0 40.0 30.0 41.0 30-31 36.2858196987983 38.0 35.0 40.0 30.0 41.0 32-33 36.04716520948041 38.0 34.5 40.0 29.5 41.0 34-35 36.23876115441129 38.0 35.0 40.0 30.0 41.0 36-37 36.29056298221066 38.0 35.0 40.0 30.0 41.0 38-39 36.25241941655263 38.0 35.0 40.0 30.0 41.0 40-41 36.15129257144958 38.0 35.0 40.0 30.0 41.0 42-43 36.00674127665505 38.0 35.0 40.0 29.0 41.0 44-45 35.82401606260878 38.0 34.5 40.0 28.5 41.0 46-47 35.60174118473924 38.0 34.0 40.0 28.0 41.0 48-49 35.39846573271652 38.0 34.0 40.0 27.0 41.0 50-51 35.12375972437227 38.0 34.0 40.0 26.5 41.0 52-53 34.90840867223548 38.0 33.0 40.0 26.0 41.0 54-55 34.73525488682171 38.0 33.0 40.0 26.0 41.0 56-57 34.47873666632893 37.5 33.0 40.0 26.0 41.0 58-59 34.235453253969375 37.0 33.0 40.0 25.5 41.0 60-61 33.91824003023389 37.0 32.5 40.0 24.5 41.0 62-63 33.6151142941058 36.0 32.0 39.0 24.0 41.0 64-65 33.30056620140165 36.0 32.0 39.0 23.0 40.5 66-67 32.95356862071132 35.5 31.0 39.0 23.0 40.0 68-69 32.57492421220668 35.0 31.0 38.5 22.0 40.0 70-71 32.171362709626834 35.0 31.0 38.0 22.0 40.0 72-73 31.750488970743838 35.0 30.5 37.0 20.5 39.5 74-75 31.223834240307262 34.0 30.0 36.5 19.5 39.0 76-77 29.864889100366508 32.5 28.5 35.0 18.0 38.0 78-79 30.627520577385205 34.0 29.5 36.0 19.0 38.0 80-81 30.631808770649528 34.0 30.0 35.5 19.0 37.5 82-83 30.353634543586693 34.0 30.0 35.0 18.0 37.0 84-85 30.034184986770533 34.0 29.5 35.0 17.5 36.5 86-87 29.69858838688605 34.0 29.0 35.0 14.0 36.0 88-89 29.353600342354333 34.0 29.0 35.0 8.5 36.0 90-91 29.01583474306538 34.0 29.0 35.0 6.5 35.0 92-93 28.664656275477245 33.5 29.0 35.0 2.0 35.0 94-95 28.23960421478887 33.0 28.5 35.0 2.0 35.0 96-97 27.777245877362077 33.0 27.0 35.0 2.0 35.0 98-99 27.274808612041312 33.0 27.0 35.0 2.0 35.0 100-101 26.06105604000176 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.48705266987788676 1101 2 0.4798793935237704 1101 3 0.4355077909067049 1101 4 0.34947280444250595 1101 5 0.4966969030404016 1101 6 0.48121832160224187 1101 7 0.45545640564365186 1101 8 0.3936528654374598 1101 9 0.6571556815694493 1101 10-11 0.6104419896579216 1101 12-13 0.6275748311397464 1101 14-15 0.8179921639903327 1101 16-17 0.7195538801907162 1101 18-19 0.715246877936913 1101 20-21 0.737661645399065 1101 22-23 0.7663630180524876 1101 24-25 0.770766853405668 1101 26-27 0.6251130132784013 1101 28-29 0.7704130801256994 1101 30-31 0.690936269334415 1101 32-33 0.525323528896898 1101 34-35 0.599909256709175 1101 36-37 0.73696930761826 1101 38-39 0.870297222936891 1101 40-41 0.727358234185516 1101 42-43 0.6337800705336889 1101 44-45 0.7528676129915581 1101 46-47 0.7806851346139112 1101 48-49 0.9774639446014106 1101 50-51 0.9452456211387457 1101 52-53 0.8543174692090787 1101 54-55 0.9750540014419897 1101 56-57 1.046806066327484 1101 58-59 0.9372368461362512 1101 60-61 0.8338376156767211 1101 62-63 0.9054191134059337 1101 64-65 0.8701524139165144 1101 66-67 0.8704826531786267 1101 68-69 0.8802823734196608 1101 70-71 0.8462497012393371 1101 72-73 0.7174718656642618 1101 74-75 0.8347236547871404 1101 76-77 0.8887126618252488 1101 78-79 0.8498093600667893 1101 80-81 0.7758697000700749 1101 82-83 0.9163415934306158 1101 84-85 1.1071905694878623 1101 86-87 0.9326960367440336 1101 88-89 0.9696147713301748 1101 90-91 0.8315758235003692 1101 92-93 0.9502737986654957 1101 94-95 0.983650292327038 1101 96-97 1.0925961913459084 1101 98-99 0.919774494686191 1101 100-101 1.178756112626349 1102 1 0.4403316237757018 1102 2 0.5371024322970541 1102 3 0.4196967837984147 1102 4 0.2759058515532047 1102 5 0.41903000430132664 1102 6 0.4046344274272542 1102 7 0.4076953313489682 1102 8 0.5409400536080682 1102 9 0.6130089201255515 1102 10-11 0.6378215197256196 1102 12-13 0.6297186358092759 1102 14-15 0.7327907910385107 1102 16-17 0.5975869376157448 1102 18-19 0.7170203695378987 1102 20-21 0.7247443806445588 1102 22-23 0.7820472448645859 1102 24-25 0.7127781189452023 1102 26-27 0.6275726942791167 1102 28-29 0.6913659235856997 1102 30-31 0.7556264526716134 1102 32-33 0.7165655173849643 1102 34-35 0.7266437114393014 1102 36-37 0.6987504203624653 1102 38-39 0.8176132052764657 1102 40-41 0.7167452192211385 1102 42-43 0.7096634498517318 1102 44-45 0.8433378891004182 1102 46-47 0.8762173110233178 1102 48-49 0.8286718671522593 1102 50-51 0.7722134375665988 1102 52-53 0.6897623749332453 1102 54-55 0.7370293673857944 1102 56-57 0.7112995611373734 1102 58-59 0.8863886358156208 1102 60-61 0.7536215018294428 1102 62-63 0.8282027502957021 1102 64-65 0.7565486732531284 1102 66-67 0.6740462539109373 1102 68-69 0.6716679029888368 1102 70-71 0.6194734161742019 1102 72-73 0.7867712648545826 1102 74-75 0.9703635299837572 1102 76-77 0.9915744584094881 1102 78-79 1.018538892569822 1102 80-81 0.8354607369180158 1102 82-83 1.0207942320365788 1102 84-85 0.9753526706313167 1102 86-87 1.0396620768152047 1102 88-89 1.0802352355322853 1102 90-91 1.0058177919327917 1102 92-93 1.0787874751794782 1102 94-95 1.1032406415530325 1102 96-97 0.9661511108511647 1102 98-99 1.094409696918671 1102 100-101 1.3977128468805269 1103 1 0.45188080736789615 1103 2 0.4434910496534137 1103 3 0.40888327233973953 1103 4 0.36328284731730065 1103 5 0.2870260942328997 1103 6 0.4557259881962352 1103 7 0.5082984956080452 1103 8 0.45283897109838733 1103 9 0.587481965996453 1103 10-11 0.5778157270316626 1103 12-13 0.6089286571698338 1103 14-15 0.606607090231158 1103 16-17 0.6639082149047653 1103 18-19 0.5736873982479409 1103 20-21 0.5909164960639828 1103 22-23 0.5553936117593352 1103 24-25 0.6355463025736015 1103 26-27 0.8390407801558055 1103 28-29 0.7136301428391718 1103 30-31 0.774648935565061 1103 32-33 0.726484781712827 1103 34-35 0.7671049546962365 1103 36-37 0.7718795612335398 1103 38-39 0.7615573057797036 1103 40-41 0.7889533217518263 1103 42-43 0.8446368396638348 1103 44-45 0.8819534352428491 1103 46-47 0.8751724538494372 1103 48-49 1.0000298918436172 1103 50-51 0.8862361014817353 1103 52-53 0.8537491965544746 1103 54-55 0.8692114027936384 1103 56-57 0.9776628354576289 1103 58-59 0.8627073788010264 1103 60-61 0.8141479128934819 1103 62-63 0.741245724844056 1103 64-65 0.6894562435800609 1103 66-67 0.6396767524446645 1103 68-69 0.7600774464521436 1103 70-71 0.58233283473254 1103 72-73 0.6897820175427576 1103 74-75 0.8083498447442707 1103 76-77 0.8827094643832005 1103 78-79 0.9510054940686778 1103 80-81 0.6714659503425828 1103 82-83 0.772365138231141 1103 84-85 0.8809824450782955 1103 86-87 0.827332327711261 1103 88-89 0.8503000412960198 1103 90-91 0.9028627939240295 1103 92-93 0.8882158086938325 1103 94-95 0.756024393046463 1103 96-97 0.6424995413931427 1103 98-99 0.8112787458307267 1103 100-101 1.0860688079246579 1104 1 0.3899834917567411 1104 2 0.5512231004190831 1104 3 0.43866648365539973 1104 4 0.2612662781858006 1104 5 0.3371013582096509 1104 6 0.3959989287037331 1104 7 0.5045411409828233 1104 8 0.49424947962382504 1104 9 0.5342844711101336 1104 10-11 0.5502604241987967 1104 12-13 0.4999340196294568 1104 14-15 0.5154695222132091 1104 16-17 0.5819462180795938 1104 18-19 0.6403133923563402 1104 20-21 0.6252746199139025 1104 22-23 0.610814118626287 1104 24-25 0.5918665022245264 1104 26-27 0.6702381328856077 1104 28-29 0.6590068181142001 1104 30-31 0.6812487886699969 1104 32-33 0.9321597487404105 1104 34-35 0.7157285637342383 1104 36-37 0.6688306988805337 1104 38-39 0.672520495140077 1104 40-41 0.6585171381803789 1104 42-43 0.6506082030237792 1104 44-45 0.7686815451140347 1104 46-47 0.707618177702507 1104 48-49 0.7042449904449555 1104 50-51 0.7656090333018923 1104 52-53 0.7653122074294529 1104 54-55 0.8658075440340269 1104 56-57 0.8356131754486285 1104 58-59 0.9339759677396771 1104 60-61 0.8191920998766946 1104 62-63 0.7233169452577286 1104 64-65 0.7464879365851687 1104 66-67 0.775243285642162 1104 68-69 0.8862100784800049 1104 70-71 0.7387058692212918 1104 72-73 0.8309113720732064 1104 74-75 0.9752473577950269 1104 76-77 1.138800160239338 1104 78-79 1.015610417422625 1104 80-81 0.8986415373305228 1104 82-83 0.9576591258003404 1104 84-85 0.9414352925522564 1104 86-87 0.9349824015536718 1104 88-89 0.9829067179136395 1104 90-91 0.9101401807713927 1104 92-93 0.9837017865332136 1104 94-95 0.9075629214106335 1104 96-97 1.0106223646215184 1104 98-99 1.1034033539131158 1104 100-101 1.3829877234284034 1105 1 0.11720770983306039 1105 2 0.2324419736847254 1105 3 0.2670913986186072 1105 4 0.14003071181436155 1105 5 0.1610101469790095 1105 6 0.16258801684283242 1105 7 0.16289624155627536 1105 8 0.12553327750651988 1105 9 0.3027851586951442 1105 10-11 0.16454613399518792 1105 12-13 0.1752542117510174 1105 14-15 0.3455095391577174 1105 16-17 0.13781155700745984 1105 18-19 0.20451334257870712 1105 20-21 0.2847362044013835 1105 22-23 0.395321426888124 1105 24-25 0.2644492386871562 1105 26-27 0.3455279996173033 1105 28-29 0.365617784529384 1105 30-31 0.4485006358476298 1105 32-33 0.4646134153157391 1105 34-35 0.4290123729300248 1105 36-37 0.39551015982394944 1105 38-39 0.2610641913557288 1105 40-41 0.266505694352432 1105 42-43 0.044145186800797376 1105 44-45 0.2261441364673047 1105 46-47 0.26113180621553767 1105 48-49 0.3138178518293202 1105 50-51 0.32456740486931324 1105 52-53 0.21903566250010442 1105 54-55 0.2660962480305713 1105 56-57 0.31104756701760294 1105 58-59 0.3569857736823181 1105 60-61 0.33343199162290205 1105 62-63 0.11455469882826463 1105 64-65 0.14364856876112952 1105 66-67 0.10753272373311518 1105 68-69 0.3694138977821666 1105 70-71 0.2525597014706591 1105 72-73 0.0734627963923451 1105 74-75 0.22203679462896986 1105 76-77 0.4190358309426898 1105 78-79 0.4095843696411272 1105 80-81 0.3732320031536531 1105 82-83 0.3508080074376778 1105 84-85 0.4462683426391152 1105 86-87 0.46461439273448235 1105 88-89 0.32582449808253244 1105 90-91 0.14674185787544047 1105 92-93 0.37561455499933416 1105 94-95 0.2664709811136312 1105 96-97 0.1636059430300918 1105 98-99 0.5029012408776588 1105 100-101 0.6956949731135218 1106 1 -0.04942229401339304 1106 2 0.06334194326505838 1106 3 0.1349135516772897 1106 4 0.02045314292387701 1106 5 0.07752356305719132 1106 6 0.025787826279966453 1106 7 -0.025951431339301223 1106 8 0.01809623490139245 1106 9 0.10426101528527454 1106 10-11 0.07879834444899103 1106 12-13 0.038831595860891355 1106 14-15 0.1294257380068018 1106 16-17 0.15544113598420495 1106 18-19 0.11744396311474503 1106 20-21 0.2269165276543248 1106 22-23 0.2634724454441155 1106 24-25 0.08209324186487521 1106 26-27 0.17414743702946822 1106 28-29 0.2604532606474308 1106 30-31 0.1789032061836835 1106 32-33 0.0728946979194589 1106 34-35 0.07851198828466721 1106 36-37 0.17058988574527945 1106 38-39 0.19480352876010443 1106 40-41 0.12294247047123008 1106 42-43 0.08395456737092388 1106 44-45 0.1295103175925334 1106 46-47 0.02368541773378041 1106 48-49 0.05275945487028366 1106 50-51 0.11861919749267003 1106 52-53 0.023130815717173903 1106 54-55 0.03898971760713721 1106 56-57 0.19507369147677167 1106 58-59 0.14097362215718334 1106 60-61 0.05902790910264599 1106 62-63 0.15334040108259472 1106 64-65 0.17006104057265503 1106 66-67 0.11339809408625712 1106 68-69 0.2744039799655411 1106 70-71 -0.008948548292394776 1106 72-73 -0.029331004783713865 1106 74-75 0.27235447272856916 1106 76-77 0.21679077790939516 1106 78-79 0.2587691351708692 1106 80-81 0.12069001270190682 1106 82-83 0.10503989202395303 1106 84-85 0.15761981170784622 1106 86-87 0.07474002469675511 1106 88-89 0.1482702831990217 1106 90-91 0.36596504394574936 1106 92-93 0.44265409302265724 1106 94-95 0.2052564319657577 1106 96-97 0.13747928408985288 1106 98-99 0.11591312717347435 1106 100-101 0.47935207057461326 1107 1 0.025998454426719775 1107 2 0.15870198251140621 1107 3 0.0805766769743208 1107 4 0.09161320976530618 1107 5 0.1497236581069359 1107 6 0.14816805593242321 1107 7 0.1542996839621722 1107 8 0.043118234318825444 1107 9 0.0459608727106513 1107 10-11 0.1678276105729566 1107 12-13 0.13922574570221968 1107 14-15 0.06641695357043176 1107 16-17 0.05692069262312316 1107 18-19 0.0873712960494828 1107 20-21 0.23570556320599678 1107 22-23 0.13886360581756918 1107 24-25 0.17753085396991963 1107 26-27 0.08587381640060698 1107 28-29 0.11815183030203258 1107 30-31 0.183615067472374 1107 32-33 0.18981217327552002 1107 34-35 0.0666652636683196 1107 36-37 0.08115466521453385 1107 38-39 -0.02704043634744835 1107 40-41 0.055477385922948486 1107 42-43 0.11393571072741082 1107 44-45 0.19198314409608486 1107 46-47 0.19651529401580348 1107 48-49 0.2307488460919842 1107 50-51 0.3114886267342669 1107 52-53 0.21540695056517478 1107 54-55 0.1431092043384936 1107 56-57 0.08885713135781259 1107 58-59 0.23751427125498026 1107 60-61 0.13138498954232603 1107 62-63 -0.03297887408643163 1107 64-65 0.08908785379990292 1107 66-67 0.16531549278928992 1107 68-69 0.3579562343309348 1107 70-71 0.26991453407894284 1107 72-73 0.33638332473229937 1107 74-75 0.3924972006563294 1107 76-77 0.3117290722263384 1107 78-79 0.34195023560165794 1107 80-81 0.4223572159397335 1107 82-83 0.5216996599186032 1107 84-85 0.5314756120420512 1107 86-87 0.5560904976459042 1107 88-89 0.4089987935241801 1107 90-91 0.2959346893488686 1107 92-93 0.24447843495645927 1107 94-95 0.3002727590595349 1107 96-97 0.22572592285482074 1107 98-99 0.13606781297273685 1107 100-101 0.6297111133316697 1108 1 0.2275528503786468 1108 2 0.35281330445953785 1108 3 0.3358407503456071 1108 4 0.12769796624854735 1108 5 0.23390455627265538 1108 6 0.14329930197922636 1108 7 0.2218281469602772 1108 8 0.32176940446430535 1108 9 0.343479848042719 1108 10-11 0.3325746061453785 1108 12-13 0.2809382694213838 1108 14-15 0.4185160996804882 1108 16-17 0.5390743928128785 1108 18-19 0.5367759450058358 1108 20-21 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-0.8948061687129503 2316 88-89 -0.9833406037732004 2316 90-91 -0.5945975235362866 2316 92-93 -0.83011601820942 2316 94-95 -0.5332517276582287 2316 96-97 -0.3724816678737781 2316 98-99 -0.18623834025778763 2316 100-101 -0.6458855868868802 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 16.0 4 39.0 5 68.0 6 171.0 7 456.0 8 1014.0 9 1834.0 10 2989.0 11 4699.0 12 6482.0 13 8018.0 14 9415.0 15 11911.0 16 14023.0 17 15639.0 18 14538.0 19 12625.0 20 12995.0 21 13976.0 22 15854.0 23 18416.0 24 22704.0 25 28265.0 26 35981.0 27 46289.0 28 58499.0 29 72087.0 30 88396.0 31 105859.0 32 126660.0 33 152677.0 34 186017.0 35 230459.0 36 296939.0 37 372226.0 38 301718.0 39 49135.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.28437579372172 21.398181698455442 14.703895297575626 23.61354721024721 2 15.595917257513417 21.907075453951578 39.4229026222993 23.07410466623572 3 18.32373392069882 26.248165907948245 31.071913509520176 24.35618666183276 4 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21.914608000102604 24-25 22.100341522943943 27.14394517677131 28.920368956789233 21.835344343495514 26-27 22.00196917383624 27.33635250520296 28.770672905091466 21.891005415869337 28-29 22.011901178916986 27.45928341547333 28.750230858417098 21.77858454719259 30-31 22.257223907462155 27.2288242298311 28.675551052081396 21.838400810625348 32-33 22.134677976458626 27.34928942489695 28.4838106812152 22.032221917429226 34-35 22.28813521275147 27.32936729443873 28.615233582901467 21.767263909908337 36-37 22.324714925617204 27.203721972183537 28.58449840909844 21.88706469310082 38-39 22.196027246574836 27.254654922463207 28.584364043516196 21.964953787445765 40-41 22.346415886148357 27.23403236535952 28.633664009740734 21.785887738751384 42-43 22.278333534638577 27.26443278417976 28.337949102196436 22.11928457898523 44-45 22.175647780930248 27.328501163790964 28.414349773484037 22.08150128179475 46-47 22.39282623715642 27.338181171416455 28.108197315674982 22.160795275752147 48-49 22.27946498927473 27.482685007155165 28.01578455227719 22.22206545129291 50-51 22.35178791301027 27.4910405703194 27.9463742821694 22.21079723450093 52-53 22.47711179989397 27.467743511864395 27.87532445757281 22.17982023066883 54-55 22.278433869347722 27.436597808764855 27.972262997818 22.312705324069423 56-57 22.315912656182594 27.534398330118787 27.822467376046934 22.327221637651682 58-59 22.285352779492936 27.518527957417767 27.915576996426967 22.280542266662334 60-61 22.28368618376168 27.606363070579356 27.933884120768308 22.17606662489066 62-63 22.22573592073724 27.528327767877375 27.95537051344621 22.29056579793918 64-65 22.018678806521493 27.459959619405623 27.915139984063448 22.60622159000944 66-67 22.11356989341187 27.554631443711493 27.80706461964053 22.52473404323611 68-69 22.178564038766414 27.478357958731355 27.84519796714488 22.49788003535735 70-71 22.14430729277321 27.27004701212114 27.85942620521915 22.7262194898865 72-73 22.144978625508426 27.38499646521418 27.778197269926086 22.69182763935131 74-75 22.061620656964713 27.59146367596461 27.63753623660467 22.709379430466008 76-77 22.096511274642545 27.432179872855418 27.672331377774118 22.79897747472792 78-79 22.079087001516292 27.480337701355385 27.61612625941643 22.824449037711894 80-81 22.14010773900919 27.499525988546342 27.548327257935124 22.81203901450935 82-83 22.142476348779034 27.411646460151072 27.693699748236178 22.75217744283371 84-85 22.176340309174932 27.434695404828318 27.409194105501843 22.979770180494903 86-87 22.01514088556396 27.546121430620452 27.483105660004693 22.9556320238109 88-89 22.028975345988957 27.305552752948582 27.465636238335218 23.199835662727246 90-91 21.997028147941027 27.312329423665492 27.588222983391447 23.10241944500203 92-93 21.999710948426955 27.188186684557074 27.66484384587394 23.147258521142028 94-95 21.866226432275912 27.11047657239648 27.61664209123591 23.406654904091692 96-97 21.898707065162327 26.97741906385242 27.64197038429787 23.481903486687383 98-99 21.665689793967502 27.118627401941858 27.49407143012881 23.72161137396183 100-101 21.638563941555226 27.026711589985364 27.546741327956898 23.78798314050251 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 249.0 1 193.0 2 174.0 3 212.0 4 181.5 5 203.5 6 324.5 7 631.5 8 1007.5 9 1204.0 10 1114.5 11 928.5 12 965.0 13 1079.0 14 1209.5 15 1419.5 16 1600.5 17 1754.5 18 2038.5 19 2331.0 20 2656.5 21 3192.5 22 3904.5 23 4922.0 24 6412.5 25 8510.0 26 11518.0 27 15525.5 28 20174.5 29 25923.5 30 32436.0 31 39221.5 32 46977.5 33 51985.5 34 54973.0 35 58561.5 36 63488.0 37 68636.0 38 70908.0 39 71915.0 40 73423.5 41 74954.5 42 76415.0 43 79056.0 44 82132.0 45 85255.5 46 87611.5 47 88618.5 48 88776.5 49 88262.5 50 86026.5 51 83103.5 52 81439.0 53 79654.5 54 76784.0 55 71178.0 56 63716.0 57 56007.5 58 48727.5 59 41711.0 60 34335.5 61 27892.5 62 22316.0 63 16822.5 64 12186.5 65 8916.5 66 6445.5 67 4851.5 68 3564.5 69 2496.0 70 1825.0 71 1313.0 72 900.5 73 638.0 74 454.5 75 306.5 76 184.5 77 103.5 78 53.0 79 31.0 80 27.0 81 17.0 82 10.0 83 5.0 84 3.0 85 2.0 86 0.5 87 0.5 88 1.0 89 1.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018425913931743747 2 0.0034628747760353674 3 0.00277885012891727 4 0.03240566765721986 5 0.0016673100773503622 6 8.550308088976216E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.137577022244054E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.550308088976216E-5 24-25 6.626488768956567E-4 26-27 0.0019238193200196486 28-29 4.275154044488108E-5 30-31 0.006925749552070735 32-33 0.022807946827344057 34-35 0.01383012333391903 36-37 6.198973364507757E-4 38-39 5.985215662283351E-4 40-41 0.002265831643578697 42-43 0.00831517461652937 44-45 0.008080041144082525 46-47 0.007909034982303 48-49 0.009747351221432886 50-51 0.19432712709220695 52-53 0.04944215652450497 54-55 0.01778464082507053 56-57 0.010602382030330508 58-59 0.020050472468649227 60-61 0.05266989782809349 62-63 0.0945450316938545 64-65 0.0996538407770178 66-67 0.10427100714506495 68-69 0.10189829665037406 70-71 0.09176618156493724 72-73 0.06902236204826051 74-75 0.24885671692965278 76-77 0.3004364504764018 78-79 0.0071181314840727 80-81 7.909034982302999E-4 82-83 0.0 84-85 2.137577022244054E-5 86-87 0.0 88-89 2.1375770222440538E-4 90-91 0.006476858377399483 92-93 0.017699137744180766 94-95 0.001710061617795243 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2339097.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.127732801092776 #Duplication Level Percentage of deduplicated Percentage of total 1 80.90071386701561 51.070786484146225 2 11.103046041798573 14.018202476097821 3 3.2685368760004385 6.190059676760225 4 1.4698659550176338 3.7115722104710493 5 0.8271882491037756 2.610925938281346 6 0.5296967558722494 2.006313316218542 7 0.3497556145588428 1.5455495287086836 8 0.2772301058421883 1.4000726436819466 9 0.18777103432201483 1.0668203714218476 >10 1.0175711144688067 11.354595391743587 >50 0.049289353357473156 2.0942734161563625 >100 0.01798057100569957 2.0093509436372696 >500 0.0011512923912652896 0.5024220463320717 >1k 2.0316924551722507E-4 0.4190555563430554 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4063 0.17369950882755184 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3392 0.14501322518903662 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2347 0.10033786542413589 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.275154044488108E-5 2 0.0 0.0 0.0 4.275154044488108E-5 4.275154044488108E-5 3 0.0 0.0 0.0 4.275154044488108E-5 4.275154044488108E-5 4 0.0 0.0 0.0 4.275154044488108E-5 4.275154044488108E-5 5 0.0 0.0 0.0 4.275154044488108E-5 8.550308088976216E-5 6 0.0 0.0 0.0 4.275154044488108E-5 8.550308088976216E-5 7 0.0 0.0 0.0 4.275154044488108E-5 8.550308088976216E-5 8 0.0 0.0 0.0 8.550308088976216E-5 8.550308088976216E-5 9 0.0 0.0 0.0 1.2825462133464325E-4 8.550308088976216E-5 10-11 0.0 0.0 0.0 1.2825462133464325E-4 8.550308088976216E-5 12-13 0.0 0.0 0.0 1.2825462133464325E-4 4.0613963422637023E-4 14-15 0.0 0.0 0.0 1.2825462133464325E-4 4.4889117467125135E-4 16-17 0.0 0.0 0.0 1.4963039155708378E-4 5.13018485338573E-4 18-19 0.0 0.0 0.0 1.7100616177952432E-4 5.771457960058946E-4 20-21 0.0 0.0 0.0 1.7100616177952432E-4 6.198973364507757E-4 22-23 0.0 0.0 0.0 1.9238193200196485E-4 6.412731066732162E-4 24-25 0.0 0.0 0.0 3.4201232355904863E-4 6.412731066732162E-4 26-27 0.0 0.0 0.0 8.977823493425027E-4 7.481519577854189E-4 28-29 0.0 0.0 0.0 0.002650595507582627 7.695277280078594E-4 30-31 0.0 0.0 0.0 0.009576345059653362 7.695277280078594E-4 32-33 0.0 0.0 0.0 0.02772437397850538 7.695277280078594E-4 34-35 0.0 0.0 0.0 0.0538883167307726 8.122792684527406E-4 36-37 0.0 0.0 0.0 0.0929632246973939 8.122792684527406E-4 38-39 0.0 0.0 0.0 0.1475141903050622 8.764065791200622E-4 40-41 0.0 0.0 0.0 0.2077297350216772 8.977823493425027E-4 42-43 0.0 0.0 0.0 0.26920645018141615 8.977823493425027E-4 44-45 0.0 0.0 0.0 0.34643710799509386 9.191581195649432E-4 46-47 0.0 0.0 0.0 0.43360749896220635 9.619096600098243E-4 48-49 0.0 0.0 0.0 0.5245400254884685 0.001026036970677146 50-51 0.0 0.0 0.0 0.617631504807197 0.001026036970677146 52-53 0.0 0.0 0.0 0.7118131484072701 0.0010901642813444675 54-55 0.0 0.0 0.0 0.8227961474021812 0.0011115400515669081 56-57 0.0 0.0 0.0 0.9513927810603835 0.0011329158217893488 58-59 0.0 0.0 0.0 1.0878984497008888 0.0011542915920117892 60-61 0.0 0.0 0.0 1.2281662539005436 0.0011542915920117892 62-63 0.0 0.0 0.0 1.3771767481211767 0.0012397946729015513 64-65 0.0 0.0 0.0 1.536062848184577 0.0012825462133464324 66-67 0.0 4.275154044488108E-5 0.0 1.7200868540295677 0.001303921983568873 68-69 0.0 4.275154044488108E-5 0.0 1.923990326181428 0.0013252977537913135 70-71 0.0 4.275154044488108E-5 0.0 2.14349811059567 0.0013252977537913135 72-73 0.0 4.275154044488108E-5 0.0 2.3686063468081913 0.001496303915570838 74-75 0.0 4.275154044488108E-5 0.0 2.602842036905695 0.0016245585369054811 76-77 0.0 4.275154044488108E-5 0.0 2.864866228292371 0.0016245585369054811 78-79 0.0 4.275154044488108E-5 0.0 3.157863910731363 0.0017528131582401243 80-81 0.0 4.275154044488108E-5 0.0 3.47892797947242 0.0018383162391298865 82-83 0.0 4.275154044488108E-5 0.0 3.816558270135869 0.00196657086046453 84-85 0.0 4.275154044488108E-5 0.0 4.165902482881215 0.00196657086046453 86-87 0.0 4.275154044488108E-5 0.0 4.549426552212243 0.002052073941354292 88-89 0.0 4.275154044488108E-5 0.0 4.966916720426729 0.0021589527924664948 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2625 0.0 55.52367 1 GTATCAA 5065 0.0 43.30436 1 TCAACGC 6365 0.0 34.007175 4 ATCAACG 6440 0.0 33.611126 3 CAACGCA 6465 0.0 33.398445 5 TATCAAC 6770 0.0 32.042885 2 AACGCAG 6830 0.0 31.543453 6 ACGCAGA 7825 0.0 27.350565 7 CGCAGAG 8130 0.0 26.20776 8 GTACATG 6625 0.0 25.582998 1 ACATGGG 6515 0.0 24.626707 3 TACATGG 6880 0.0 24.148138 2 GCAGAGT 9165 0.0 22.678572 9 CATGGGG 4585 0.0 22.258873 4 GTGGTAT 1190 0.0 21.543482 1 TATACCG 135 3.8218664E-4 21.091177 5 AGAGTAC 8520 0.0 20.942202 10-11 TATCACG 160 5.0178183E-5 20.7674 2 CAGAGTA 9030 0.0 18.813488 10-11 GAGTACT 6030 0.0 18.493734 12-13 >>END_MODULE