FastQCFastQC Report
Sat 18 Jun 2016
SRR1930949_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930949_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022612
Sequences flagged as poor quality0
Sequence length101
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37080.18332730152891408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14950.050.5112841
GTATCAA25600.048.5730481
TATCAAC33800.036.18252
TCAACGC34950.034.9803664
ATCAACG35600.034.208283
CAACGCA36050.033.1147425
AACGCAG40450.029.09346
GTACATG53850.028.7006021
GTACTAG4200.027.5687391
TACATGG54800.026.7252252
ACATGGG59650.024.5498243
ACGCAGA46600.024.2911957
GTATAAG4500.023.4933591
GTGGTAT6400.022.8116341
CTAATAC2904.9112714E-1122.5585483
CGCAGAG50400.022.2749068
CATGGGG51350.020.967114
AGTACAT37300.020.5089652
GAGTACA39950.020.0363521
GCAGAGT53200.019.1475479
Produced by FastQC (version 0.11.5)