##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930949_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2022612 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.199727382216658 28.0 10.0 31.0 2.0 33.0 2 21.163183052409458 28.0 10.0 31.0 2.0 33.0 3 21.590253592878913 28.0 10.0 31.0 2.0 33.0 4 23.48852177283631 32.0 2.0 35.0 2.0 37.0 5 23.408922719730725 32.0 2.0 35.0 2.0 37.0 6 24.07071796271356 32.0 10.0 35.0 2.0 37.0 7 24.08362997945231 32.0 10.0 35.0 2.0 37.0 8 24.734905162235762 32.0 11.0 35.0 2.0 37.0 9 25.816843269989498 32.0 16.0 37.0 2.0 39.0 10-11 25.944413214200253 32.0 15.0 37.0 2.0 39.0 12-13 25.8051358836989 32.0 11.0 37.0 2.0 39.0 14-15 25.999316725105952 32.5 10.5 38.0 2.0 40.0 16-17 25.14364124211663 32.0 10.0 38.0 2.0 40.0 18-19 24.500995742139374 31.5 8.5 37.5 2.0 40.0 20-21 23.92697536650628 31.0 2.0 37.0 2.0 39.5 22-23 23.79038490822758 30.0 2.0 37.0 2.0 39.0 24-25 23.42305766009497 30.0 2.0 37.0 2.0 39.0 26-27 23.011838899403344 30.0 2.0 37.0 2.0 39.0 28-29 22.85033436961711 30.0 2.0 37.0 2.0 39.0 30-31 22.686060648310203 30.0 2.0 37.0 2.0 39.0 32-33 22.29472706579413 30.0 2.0 36.0 2.0 39.0 34-35 19.570792371448405 22.5 2.0 35.0 2.0 38.0 36-37 17.98580325836097 16.5 2.0 34.0 2.0 38.0 38-39 17.05139146806209 15.0 2.0 33.0 2.0 38.0 40-41 18.08962940000356 15.5 2.0 33.0 2.0 38.0 42-43 17.925593984412235 15.5 2.0 33.0 2.0 38.0 44-45 16.143502560056007 10.5 2.0 31.5 2.0 37.5 46-47 16.739944932592113 14.0 2.0 31.0 2.0 37.0 48-49 16.16451524068877 8.5 2.0 32.0 2.0 37.0 50-51 14.426164039370873 7.0 2.0 29.5 2.0 36.5 52-53 16.905958730591927 16.0 2.0 32.0 2.0 37.0 54-55 18.267396317237313 20.5 2.0 34.0 2.0 38.0 56-57 18.36529324457681 21.0 2.0 34.0 2.0 38.0 58-59 17.968830403458497 18.5 2.0 34.0 2.0 38.0 60-61 17.57197030374585 17.5 2.0 33.5 2.0 37.5 62-63 17.044316458124445 12.0 2.0 33.0 2.0 37.0 64-65 16.521638356738713 8.0 2.0 32.0 2.0 36.0 66-67 16.054904746931197 7.0 2.0 32.0 2.0 36.0 68-69 15.549784387712522 2.0 2.0 31.0 2.0 35.0 70-71 14.97279804529984 2.0 2.0 31.0 2.0 35.0 72-73 14.463693975908381 2.0 2.0 30.0 2.0 34.0 74-75 13.925488675039997 2.0 2.0 30.0 2.0 34.0 76-77 13.36949795610824 2.0 2.0 29.0 2.0 34.0 78-79 12.869919193597191 2.0 2.0 29.0 2.0 34.0 80-81 12.362797461895806 2.0 2.0 28.5 2.0 34.0 82-83 11.893759653359123 2.0 2.0 27.0 2.0 33.0 84-85 11.39198200149114 2.0 2.0 26.0 2.0 33.0 86-87 10.867579891743944 2.0 2.0 25.0 2.0 32.5 88-89 10.352995532509448 2.0 2.0 24.5 2.0 32.0 90-91 9.811561238635981 2.0 2.0 21.5 2.0 32.0 92-93 9.236013135490147 2.0 2.0 18.5 2.0 31.5 94-95 8.634602682076444 2.0 2.0 9.0 2.0 31.0 96-97 7.995566129341663 2.0 2.0 2.0 2.0 31.0 98-99 7.32335588832658 2.0 2.0 2.0 2.0 31.0 100-101 6.513706781132516 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.886981766266302 1101 2 8.034326357899854 1101 3 7.67279566814997 1101 4 9.527927545206989 1101 5 9.301364915251643 1101 6 9.09126236104148 1101 7 8.482235413552292 1101 8 7.826525885960127 1101 9 8.064934200935177 1101 10-11 7.585548915077698 1101 12-13 7.6486662320683685 1101 14-15 7.970047122242125 1101 16-17 8.522791215586153 1101 18-19 9.05438532706017 1101 20-21 9.208494711467601 1101 22-23 9.320874202606404 1101 24-25 9.187634138143107 1101 26-27 9.17731288195142 1101 28-29 9.079050208189301 1101 30-31 9.033105995797765 1101 32-33 9.265952996346495 1101 34-35 11.409967172689743 1101 36-37 5.610633778454432 1101 38-39 8.384554280144542 1101 40-41 10.102697676031728 1101 42-43 10.432586958408727 1101 44-45 4.82456683086647 1101 46-47 6.484299868727565 1101 48-49 9.36293411036539 1101 50-51 -2.591065073333846 1101 52-53 6.391023841242358 1101 54-55 8.928722861528666 1101 56-57 9.389893554018904 1101 58-59 9.296457959181193 1101 60-61 9.354909072653872 1101 62-63 9.351090418128159 1101 64-65 9.09479684693948 1101 66-67 8.978574345964956 1101 68-69 8.700669512250613 1101 70-71 8.752643058024 1101 72-73 8.50993737165224 1101 74-75 8.68041342784155 1101 76-77 8.43460579288241 1101 78-79 8.38072183548906 1101 80-81 8.158740152875327 1101 82-83 7.867141966841766 1101 84-85 7.742152959793668 1101 86-87 7.718559941178377 1101 88-89 7.581218386500158 1101 90-91 7.215698200258425 1101 92-93 7.132274845226847 1101 94-95 6.879956713562738 1101 96-97 6.670135013990957 1101 98-99 6.128199815148871 1101 100-101 5.175367405169341 1102 1 6.942058370349528 1102 2 7.727416532446981 1102 3 7.659908376454734 1102 4 9.353749548512436 1102 5 9.511545785035157 1102 6 8.704183125617874 1102 7 8.62071016593433 1102 8 7.983977205000851 1102 9 7.6060688851162155 1102 10-11 7.40919192680018 1102 12-13 7.1090687434642525 1102 14-15 7.382431972044191 1102 16-17 8.188170958449334 1102 18-19 8.485520801573127 1102 20-21 8.96216452474006 1102 22-23 8.719230544671355 1102 24-25 8.5501777516801 1102 26-27 8.671903873562279 1102 28-29 8.3887812861323 1102 30-31 8.647350333883164 1102 32-33 8.660880576373415 1102 34-35 10.558424514524571 1102 36-37 1.6436259518439549 1102 38-39 3.834058114416589 1102 40-41 8.238091993545247 1102 42-43 9.338843249102105 1102 44-45 1.6038442117994762 1102 46-47 2.0852385041032875 1102 48-49 7.276995742307815 1102 50-51 -3.816455532168014 1102 52-53 4.967895939968763 1102 54-55 7.683569339553948 1102 56-57 8.257413120545092 1102 58-59 8.252288516667381 1102 60-61 8.257417446685423 1102 62-63 8.314438775516251 1102 64-65 8.250171568870492 1102 66-67 8.126101769320199 1102 68-69 8.080146451992452 1102 70-71 8.01051720463987 1102 72-73 7.927887320396893 1102 74-75 7.600071423327357 1102 76-77 7.530968638914134 1102 78-79 7.689016252723876 1102 80-81 7.445634938776736 1102 82-83 7.200165753507937 1102 84-85 6.989151325666207 1102 86-87 6.704085360809753 1102 88-89 6.763205908553903 1102 90-91 6.689998582174701 1102 92-93 6.3669087007216305 1102 94-95 6.087997178556845 1102 96-97 5.730640733437075 1102 98-99 5.207585285593944 1102 100-101 4.639909396201398 1103 1 6.9841241782217764 1103 2 7.972395244667272 1103 3 7.675976631671681 1103 4 9.022248067165943 1103 5 9.155942928277359 1103 6 8.576628603246341 1103 7 8.47323973801615 1103 8 7.714413782461367 1103 9 7.820987927078825 1103 10-11 7.324738162407034 1103 12-13 7.168990630812079 1103 14-15 7.668555827565786 1103 16-17 8.22202678898168 1103 18-19 8.456035693168655 1103 20-21 8.852893897040474 1103 22-23 9.13674146168237 1103 24-25 8.969811626047044 1103 26-27 8.876071008489955 1103 28-29 8.83457613811182 1103 30-31 8.69588060622974 1103 32-33 8.528920632054309 1103 34-35 10.346142434989002 1103 36-37 -2.4186266440383637 1103 38-39 -1.3227154470967903 1103 40-41 6.434090678981757 1103 42-43 8.807414950794694 1103 44-45 1.2645562028482686 1103 46-47 1.040812436882348 1103 48-49 7.3083650509217435 1103 50-51 -4.0947558096322485 1103 52-53 4.558843271910817 1103 54-55 7.666854772211451 1103 56-57 8.22184726051118 1103 58-59 8.219132615078752 1103 60-61 8.35635827386644 1103 62-63 8.363466710438196 1103 64-65 8.261067424189704 1103 66-67 8.147849489542045 1103 68-69 8.041056097691623 1103 70-71 7.690246823644804 1103 72-73 7.56403049588261 1103 74-75 7.3188646441914855 1103 76-77 7.1406117859683835 1103 78-79 6.923922055026244 1103 80-81 6.672864021613487 1103 82-83 6.818207152407661 1103 84-85 6.767296013616324 1103 86-87 6.334426502572619 1103 88-89 6.286848910540524 1103 90-91 5.99304900974364 1103 92-93 5.781224659817932 1103 94-95 5.292488588896317 1103 96-97 4.950520506397273 1103 98-99 4.6311204271087645 1103 100-101 4.19862462530304 1104 1 6.817302495476433 1104 2 7.583926995515299 1104 3 7.21023022104033 1104 4 8.849697542034829 1104 5 8.89066439099258 1104 6 8.285195236325578 1104 7 8.003604967869553 1104 8 7.267463116994534 1104 9 7.247519613321607 1104 10-11 6.726955830385581 1104 12-13 6.412677001493297 1104 14-15 7.113692399760605 1104 16-17 7.738954676173492 1104 18-19 8.121196692714658 1104 20-21 8.376683408346722 1104 22-23 8.321069193777795 1104 24-25 8.337193162482325 1104 26-27 8.085118895865715 1104 28-29 8.115142903467557 1104 30-31 7.888632204771454 1104 32-33 7.717601552900192 1104 34-35 9.818926874893315 1104 36-37 -2.759258106615613 1104 38-39 -1.6121060838920371 1104 40-41 5.921189888323102 1104 42-43 8.104542338971235 1104 44-45 0.7512442381547046 1104 46-47 0.20138712130764347 1104 48-49 6.608639699847007 1104 50-51 -4.461398218308361 1104 52-53 3.7288536065593973 1104 54-55 6.568317325371158 1104 56-57 7.081837519354817 1104 58-59 7.384748369822365 1104 60-61 7.131127540080115 1104 62-63 7.213553423811899 1104 64-65 7.06920058895081 1104 66-67 6.933696474108837 1104 68-69 6.680610207647113 1104 70-71 6.772373663224295 1104 72-73 6.472998426059894 1104 74-75 6.358945297812568 1104 76-77 6.5012275111468885 1104 78-79 6.21574918541692 1104 80-81 6.060698591899937 1104 82-83 5.875172988061125 1104 84-85 5.845316478366719 1104 86-87 5.536427272141495 1104 88-89 5.249781489167837 1104 90-91 5.016587655613405 1104 92-93 4.790262604556579 1104 94-95 4.099220476926453 1104 96-97 3.893768853543354 1104 98-99 3.6607558367377484 1104 100-101 3.147050480276757 1105 1 6.255511707612683 1105 2 7.114954827225194 1105 3 6.688535173100124 1105 4 8.581909759768372 1105 5 8.366052361816713 1105 6 7.679948214743682 1105 7 7.5923754546591695 1105 8 6.420157877256759 1105 9 6.9580790378446125 1105 10-11 6.51833331512039 1105 12-13 6.173009147443512 1105 14-15 6.379558510408348 1105 16-17 7.081908002960759 1105 18-19 7.630852384750952 1105 20-21 7.889067178121337 1105 22-23 7.3934877485968755 1105 24-25 7.456514899504018 1105 26-27 7.5698771501812665 1105 28-29 7.478672878713418 1105 30-31 7.23413194638 1105 32-33 7.095699251454732 1105 34-35 9.021481459682779 1105 36-37 -3.068624002531516 1105 38-39 -2.1383433531687857 1105 40-41 5.059006985213525 1105 42-43 7.341419543700912 1105 44-45 -2.787458824783661 1105 46-47 -3.649907894298506 1105 48-49 4.580755468728551 1105 50-51 -8.419533939635501 1105 52-53 0.8818911596668926 1105 54-55 5.432090022158786 1105 56-57 6.381919370332433 1105 58-59 6.726168638821811 1105 60-61 6.705024578164053 1105 62-63 6.7536761808260195 1105 64-65 6.666743525051501 1105 66-67 6.542622466949092 1105 68-69 6.396187462369216 1105 70-71 6.349959290650839 1105 72-73 6.352640097306859 1105 74-75 6.010219931725587 1105 76-77 5.638978362131207 1105 78-79 5.498165749616765 1105 80-81 5.571675851939 1105 82-83 5.482566571523728 1105 84-85 5.031452777211186 1105 86-87 4.691898308631641 1105 88-89 4.749027063930505 1105 90-91 4.623286791779757 1105 92-93 4.016072744438105 1105 94-95 3.826231904723496 1105 96-97 3.3717612718993264 1105 98-99 3.20917175987607 1105 100-101 2.9020473992477456 1106 1 5.937936993225179 1106 2 6.904003450387165 1106 3 6.468634008148896 1106 4 7.831434378184625 1106 5 7.7740135011301525 1106 6 6.93765532002568 1106 7 6.809209758501087 1106 8 6.191926987127651 1106 9 6.387698911564851 1106 10-11 6.014649374196406 1106 12-13 5.93321749285138 1106 14-15 6.131192408204928 1106 16-17 6.390308342866049 1106 18-19 6.715930772638462 1106 20-21 7.129285525506589 1106 22-23 6.6922486668184575 1106 24-25 6.9128431851131005 1106 26-27 6.893344486714778 1106 28-29 7.021658688434641 1106 30-31 6.316844719591234 1106 32-33 6.30223379854883 1106 34-35 8.320540689969466 1106 36-37 -0.062076905022678375 1106 38-39 0.9070406724758264 1106 40-41 5.966272950875247 1106 42-43 7.131189762440361 1106 44-45 -3.1065052830523605 1106 46-47 -4.232490582141352 1106 48-49 3.500717808961131 1106 50-51 -8.529602461916856 1106 52-53 -0.36782192805049263 1106 54-55 3.950355243744987 1106 56-57 4.9454841313248075 1106 58-59 5.344923901898959 1106 60-61 5.472821875968137 1106 62-63 5.622153161362437 1106 64-65 5.27627125017483 1106 66-67 5.195522955158095 1106 68-69 4.824506695485603 1106 70-71 4.7913304223956725 1106 72-73 4.7662535044738465 1106 74-75 4.477039533044298 1106 76-77 4.116404022764582 1106 78-79 4.214670951326864 1106 80-81 4.053967941214426 1106 82-83 3.8726679805794593 1106 84-85 3.8036410155335485 1106 86-87 3.703608655287308 1106 88-89 3.4070481852770342 1106 90-91 3.045403158202433 1106 92-93 2.8618582981223444 1106 94-95 2.7248369122750535 1106 96-97 2.350045432040063 1106 98-99 2.2226305348533133 1106 100-101 1.968334683994284 1107 1 5.646047432181284 1107 2 6.592420442021336 1107 3 6.32914629025402 1107 4 7.324142190736705 1107 5 7.603121840296236 1107 6 6.436477937763904 1107 7 6.448098786264978 1107 8 6.260073505809114 1107 9 6.087156049184422 1107 10-11 5.759996089524872 1107 12-13 5.319691553778622 1107 14-15 5.515738953550393 1107 16-17 6.52089236779689 1107 18-19 6.49257769127993 1107 20-21 6.9795396667591305 1107 22-23 6.999028197198754 1107 24-25 6.78138112298598 1107 26-27 6.756930419788137 1107 28-29 6.753939418694948 1107 30-31 6.705852902106319 1107 32-33 6.314845662362451 1107 34-35 8.515024783226806 1107 36-37 -0.1424380355762871 1107 38-39 0.4415707611336259 1107 40-41 5.915700716432024 1107 42-43 7.129714284988108 1107 44-45 -3.1904102258914104 1107 46-47 -4.214855887277505 1107 48-49 3.471728832858741 1107 50-51 -8.476188428320214 1107 52-53 -0.15746942163446676 1107 54-55 4.269486663936302 1107 56-57 5.5948333630683 1107 58-59 5.771439167039112 1107 60-61 5.707550069815419 1107 62-63 5.584622331112129 1107 64-65 5.582708731426706 1107 66-67 5.463351588961379 1107 68-69 5.41185504398409 1107 70-71 5.223110786025979 1107 72-73 5.045713683455949 1107 74-75 4.911000303633905 1107 76-77 4.720067406426216 1107 78-79 4.449266033485314 1107 80-81 4.407614868188395 1107 82-83 4.156772070170501 1107 84-85 4.107096919943107 1107 86-87 4.062887519067596 1107 88-89 3.712620336395256 1107 90-91 3.596043302521334 1107 92-93 3.3741364453076255 1107 94-95 3.0946790947234746 1107 96-97 3.0440868612304763 1107 98-99 2.636735374369361 1107 100-101 2.351570943701647 1108 1 6.097317834229489 1108 2 6.327892342991262 1108 3 6.607822528600561 1108 4 7.737130893393388 1108 5 7.9553523387305916 1108 6 7.42569787513801 1108 7 7.512027609446182 1108 8 6.690591183923729 1108 9 5.2494259345794525 1108 10-11 6.025593890715893 1108 12-13 5.366085963047421 1108 14-15 5.97977518620748 1108 16-17 6.148052532372887 1108 18-19 6.418796271132127 1108 20-21 7.331303190208285 1108 22-23 7.380321168833028 1108 24-25 6.855326638038996 1108 26-27 7.376001092504037 1108 28-29 7.428152777630853 1108 30-31 7.106778589809345 1108 32-33 6.9828521759575395 1108 34-35 8.968681202590215 1108 36-37 -3.1693645557811934 1108 38-39 -2.3614916116640607 1108 40-41 4.79969513574105 1108 42-43 6.922055163131169 1108 44-45 -2.8704233827678127 1108 46-47 -3.954071013711113 1108 48-49 4.049533035947366 1108 50-51 -8.499531320783145 1108 52-53 0.3575669637176837 1108 54-55 4.976484490646225 1108 56-57 6.000837625979923 1108 58-59 6.450882645809269 1108 60-61 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3.767667520640412 1109 22-23 5.009172159654362 1109 24-25 4.993662325421294 1109 26-27 3.9537848084233467 1109 28-29 3.910601645990738 1109 30-31 3.804042272984045 1109 32-33 3.5221040337475316 1109 34-35 5.423421536144108 1109 36-37 -4.5997408006701495 1109 38-39 -3.707573074482813 1109 40-41 2.1169437402777866 1109 42-43 2.539661646733414 1109 44-45 -4.554435466885829 1109 46-47 -5.845027754350056 1109 48-49 0.7540633077445875 1109 50-51 -8.902691472460244 1109 52-53 -1.8864553424868262 1109 54-55 1.4970519861077882 1109 56-57 2.8333068536822026 1109 58-59 3.511171536394773 1109 60-61 3.695218131712931 1109 62-63 3.7721159891537326 1109 64-65 3.4920440941867277 1109 66-67 3.5963170887077016 1109 68-69 3.81857434890545 1109 70-71 3.690906321287267 1109 72-73 3.8527278894427592 1109 74-75 3.9001809226090423 1109 76-77 3.839952616979234 1109 78-79 3.854120802638027 1109 80-81 3.8105829047180766 1109 82-83 3.6057732117230117 1109 84-85 3.3219719833626904 1109 86-87 3.20436392698236 1109 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2313 74-75 -11.664549717121597 2313 76-77 -11.121678859213759 2313 78-79 -10.646715549539948 2313 80-81 -10.149674115748368 2313 82-83 -9.677283467636023 2313 84-85 -9.185833713219184 2313 86-87 -8.663434475894578 2313 88-89 -8.153286418161718 2313 90-91 -7.61152821702035 2313 92-93 -7.039638143440067 2313 94-95 -6.502352868746845 2313 96-97 -5.866752182582103 2313 98-99 -5.200988344929342 2313 100-101 -4.396097920222686 2314 1 -19.942993663709128 2314 2 -18.912602389028894 2314 3 -19.292314896960594 2314 4 -21.18362036634092 2314 5 -21.09961788573116 2314 6 -21.773111103331985 2314 7 -21.774275642958766 2314 8 -22.42851096907673 2314 9 -23.517866781865806 2314 10-11 -23.640003910475127 2314 12-13 -23.505879222477088 2314 14-15 -23.697274745079742 2314 16-17 -22.849198956404024 2314 18-19 -22.2124451397703 2314 20-21 -21.648999146026256 2314 22-23 -21.4813370996049 2314 24-25 -21.136198785689817 2314 26-27 -20.73325222861369 2314 28-29 -20.575740946601854 2314 30-31 -20.397115134423363 2314 32-33 -20.00775707736358 2314 34-35 -17.389541426818855 2314 36-37 -15.816638948818298 2314 38-39 -14.903406407816146 2314 40-41 -15.907130333796285 2314 42-43 -15.753162427340659 2314 44-45 -13.980592874293237 2314 46-47 -14.54248145805376 2314 48-49 -14.00635335892208 2314 50-51 -12.267969250238023 2314 52-53 -14.703816453597938 2314 54-55 -15.998549865744064 2314 56-57 -16.09192462779928 2314 58-59 -15.689291426568193 2314 60-61 -15.304550386805587 2314 62-63 -14.781587714549971 2314 64-65 -14.272770720628088 2314 66-67 -13.826831059440446 2314 68-69 -13.32193491035381 2314 70-71 -12.75816775278681 2314 72-73 -12.242642480927612 2314 74-75 -11.710004262576142 2314 76-77 -11.145001086724468 2314 78-79 -10.666944012176787 2314 80-81 -10.189417095281923 2314 82-83 -9.72131012161032 2314 84-85 -9.230574312933605 2314 86-87 -8.706747184128751 2314 88-89 -8.19445728917552 2314 90-91 -7.6555548709946475 2314 92-93 -7.082712869760867 2314 94-95 -6.502352868746845 2314 96-97 -5.875319531463588 2314 98-99 -5.22216873521968 2314 100-101 -4.42536969556776 2315 1 -19.942993663709128 2315 2 -18.912602389028894 2315 3 -19.292314896960594 2315 4 -17.641047045621907 2315 5 -21.09961788573116 2315 6 -21.773111103331985 2315 7 -21.774275642958766 2315 8 -22.42851096907673 2315 9 -20.19809383957631 2315 10-11 -19.058641564212117 2315 12-13 -16.538755286810105 2315 14-15 -16.507586949431683 2315 16-17 -19.948300186299956 2315 18-19 -22.2124451397703 2315 20-21 -21.648999146026256 2315 22-23 -21.4813370996049 2315 24-25 -21.136198785689817 2315 26-27 -20.73325222861369 2315 28-29 -20.575740946601854 2315 30-31 -20.397115134423363 2315 32-33 -20.00775707736358 2315 34-35 -17.389541426818855 2315 36-37 -15.816638948818298 2315 38-39 -14.903406407816146 2315 40-41 -15.907130333796285 2315 42-43 -15.753162427340659 2315 44-45 -13.980592874293237 2315 46-47 -14.54248145805376 2315 48-49 -14.00635335892208 2315 50-51 -12.267969250238023 2315 52-53 -14.703816453597938 2315 54-55 -15.998549865744064 2315 56-57 -16.09192462779928 2315 58-59 -15.689291426568193 2315 60-61 -15.304550386805587 2315 62-63 -14.781587714549971 2315 64-65 -14.272770720628088 2315 66-67 -13.826831059440446 2315 68-69 -13.32193491035381 2315 70-71 -12.75816775278681 2315 72-73 -12.242642480927612 2315 74-75 -11.710004262576142 2315 76-77 -11.145001086724468 2315 78-79 -10.666944012176787 2315 80-81 -10.189417095281923 2315 82-83 -9.72131012161032 2315 84-85 -9.230574312933605 2315 86-87 -8.706747184128751 2315 88-89 -8.19445728917552 2315 90-91 -7.6555548709946475 2315 92-93 -7.082712869760867 2315 94-95 -6.502352868746845 2315 96-97 -5.875319531463588 2315 98-99 -5.22216873521968 2315 100-101 -4.42536969556776 2316 1 -19.942993663709128 2316 2 -18.912602389028894 2316 3 -19.292314896960594 2316 4 -21.18362036634092 2316 5 -21.09961788573116 2316 6 -21.773111103331985 2316 7 -21.774275642958766 2316 8 -22.42851096907673 2316 9 -23.517866781865806 2316 10-11 -23.640003910475127 2316 12-13 -23.505879222477088 2316 14-15 -23.697274745079742 2316 16-17 -22.849198956404024 2316 18-19 -22.2124451397703 2316 20-21 -21.648999146026256 2316 22-23 -21.4813370996049 2316 24-25 -21.136198785689817 2316 26-27 -20.73325222861369 2316 28-29 -20.575740946601854 2316 30-31 -20.397115134423363 2316 32-33 -20.00775707736358 2316 34-35 -17.389541426818855 2316 36-37 -15.816638948818298 2316 38-39 -14.903406407816146 2316 40-41 -15.907130333796285 2316 42-43 -15.753162427340659 2316 44-45 -13.980592874293237 2316 46-47 -14.54248145805376 2316 48-49 -14.00635335892208 2316 50-51 -12.267969250238023 2316 52-53 -14.703816453597938 2316 54-55 -15.998549865744064 2316 56-57 -16.09192462779928 2316 58-59 -15.689291426568193 2316 60-61 -15.304550386805587 2316 62-63 -14.781587714549971 2316 64-65 -14.272770720628088 2316 66-67 -13.826831059440446 2316 68-69 -13.32193491035381 2316 70-71 -12.75816775278681 2316 72-73 -12.242642480927612 2316 74-75 -11.710004262576142 2316 76-77 -11.145001086724468 2316 78-79 -10.666944012176787 2316 80-81 -10.189417095281923 2316 82-83 -9.72131012161032 2316 84-85 -9.230574312933605 2316 86-87 -8.706747184128751 2316 88-89 -8.19445728917552 2316 90-91 -7.6555548709946475 2316 92-93 -7.082712869760867 2316 94-95 -6.502352868746845 2316 96-97 -5.875319531463588 2316 98-99 -5.22216873521968 2316 100-101 -4.42536969556776 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 374756.0 3 24930.0 4 46237.0 5 34457.0 6 31928.0 7 39077.0 8 49015.0 9 56711.0 10 57778.0 11 52163.0 12 44015.0 13 37952.0 14 35638.0 15 36985.0 16 40270.0 17 43004.0 18 44658.0 19 47186.0 20 49182.0 21 50913.0 22 52829.0 23 55793.0 24 57610.0 25 59816.0 26 61967.0 27 64308.0 28 65890.0 29 67882.0 30 66458.0 31 63964.0 32 59525.0 33 54396.0 34 44818.0 35 31456.0 36 15391.0 37 3499.0 38 155.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.484005081418175 17.085517958193787 12.65186511144474 25.778611848943296 2 17.108012711369234 18.999702195086094 37.72069920070166 26.17158589284301 3 20.752807869965974 22.719703117351287 27.285752530615724 29.241736482067015 4 14.523997844148754 15.045080071293263 34.069194680112524 36.36172740444545 5 15.861838928562353 35.658784213067676 32.47744542428016 16.001931434089812 6 35.24660259589119 32.51413204673499 16.632703423141574 15.606561934232241 7 30.736128169405223 29.769896038976963 19.874542621463124 19.619433170154693 8 28.138859144183975 33.00812039807591 18.98989978437742 19.863120673362687 9 28.115568527445284 14.778711307547116 18.229792405021996 38.87592775998561 10-11 27.658159797448867 23.93622716521205 26.01953020593774 22.386082831401346 12-13 27.73866551841685 21.468606388677436 26.008627251966505 24.784100840939207 14-15 24.907826149160286 22.5464192397449 23.680051504716594 28.86570310637822 16-17 25.392265501793364 25.38012365148703 24.07893598314097 25.148674863578634 18-19 25.395606448132114 24.88496837172347 25.275009006497978 24.444416173646438 20-21 26.141576601480228 24.12706385457488 25.263800742147602 24.467558801797292 22-23 25.987587667311363 23.8617041161056 25.01906720590909 25.13164101067395 24-25 25.766059715574745 24.102564851558174 24.858101387657573 25.273274045209508 26-27 25.53728265010051 24.302626790871205 24.93268532611071 25.227405232917572 28-29 25.472695697211243 24.522391924845728 24.975807248511806 25.029105129431223 30-31 25.831023322246615 24.2499405710399 24.762592415975764 25.156443690737714 32-33 25.709156497477665 24.39035594510047 24.68024124228504 25.220246315136823 34-35 25.867226900517228 24.276851136072207 24.884372293856227 24.971549669554342 36-37 26.293666902294248 24.416172021996527 24.472826595423975 24.817334480285247 38-39 26.18529434039281 24.419996482882294 24.379979641793113 25.014729534931785 40-41 26.252492490462366 24.11176497750232 24.61574073934352 25.020001792691794 42-43 26.131215478357923 24.132717464676038 24.50239543000614 25.233671626959904 44-45 26.28698494398945 24.324720914744958 24.36561418106235 25.02267996020324 46-47 25.789591336530503 24.126050160656554 24.575140547923706 25.509217954889234 48-49 26.11300032746417 24.354328824403638 24.315096098138493 25.217574749993705 50-51 26.147097436244838 24.488807681041333 24.428842668995017 24.935252213718815 52-53 25.532042839042894 24.330218875114596 24.650991089258586 25.486747196583927 54-55 25.236903814273305 24.183720793107593 24.84462488859256 25.734750504026543 56-57 25.465480093782446 24.391103353227006 24.546261035322335 25.597155517668213 58-59 25.540434195653024 24.3928075024865 24.617512438208283 25.44924586365219 60-61 25.612557351325727 24.38099668975217 24.62917319172435 25.377272767197745 62-63 25.36351415756212 24.431560904178724 24.578447611115813 25.626477327143345 64-65 25.326317165492657 24.192190863004697 24.6774955537387 25.803996417763948 66-67 25.501903777799402 24.378312064309384 24.346868371301078 25.77291578659014 68-69 25.283319977416358 24.416684120421806 24.516028238658095 25.78396766350374 70-71 25.146481119246307 24.21478443728109 24.58352967808053 26.055204765392077 72-73 25.428176741789883 24.322247053737886 24.32744887610038 25.922127328371854 74-75 25.43662447858826 24.401832885988068 24.30489338464464 25.85664925077904 76-77 25.419391352641906 24.162094653348642 24.341103667024854 26.0774103269846 78-79 25.423164998048577 24.276903406666733 24.268482212675714 26.031449382608972 80-81 25.465488904275336 24.436415517928705 24.154032297545864 25.944063280250102 82-83 25.698441592525235 24.414912888573685 24.2065255097788 25.680120009122277 84-85 25.596213912826006 24.362135280610456 24.026647721573084 26.015003084990457 86-87 25.333825116156167 24.422274458855394 24.247902582960826 25.995997842027613 88-89 25.398790263500203 24.300677009526 24.154604906277857 26.145927820695935 90-91 25.55874029904805 24.31600148248737 23.94154562088404 26.183712597580545 92-93 25.56271666083017 24.179953621405026 24.15597716172678 26.10135255603802 94-95 25.57463024386115 24.02667400156238 24.066902712222458 26.33179304235401 96-97 25.368792760613136 24.15694718616781 24.07363141802163 26.40062863519742 98-99 25.32028131218551 24.008488477947544 23.97099051047012 26.70023969939683 100-101 26.062053946757736 24.017090561477396 23.497722033853247 26.423133457911625 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 21905.0 1 24866.5 2 18523.0 3 8977.5 4 11567.0 5 16030.0 6 18033.0 7 17826.0 8 16618.0 9 15374.0 10 14129.5 11 12850.0 12 11557.5 13 10110.0 14 8514.5 15 7035.5 16 5754.0 17 4741.5 18 4038.0 19 3529.5 20 3329.5 21 3474.0 22 3721.0 23 4055.0 24 4672.0 25 5397.0 26 5976.0 27 6609.0 28 7365.0 29 8126.5 30 8921.5 31 9775.5 32 10727.0 33 11828.0 34 13041.0 35 14407.0 36 16294.0 37 18481.5 38 20946.0 39 24309.5 40 28533.5 41 33866.0 42 40283.0 43 47364.5 44 54888.5 45 62509.5 46 69813.5 47 76716.5 48 82483.0 49 86244.5 50 88185.5 51 89473.5 52 90089.0 53 89058.0 54 85911.0 55 80818.5 56 74479.5 57 67412.5 58 60109.0 59 52708.5 60 45377.5 61 38237.5 62 31640.5 63 25447.5 64 19883.5 65 15253.5 66 11502.5 67 8659.5 68 6470.0 69 4847.5 70 3572.0 71 2583.5 72 1836.5 73 1271.5 74 879.5 75 591.0 76 411.0 77 269.5 78 171.0 79 101.0 80 59.5 81 36.0 82 25.5 83 17.5 84 11.0 85 7.0 86 6.0 87 4.0 88 2.0 89 2.5 90 1.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.378041858745028 2 21.30739855197141 3 17.0794497412257 4 22.301608019728945 5 22.18181242868133 6 16.053400256697774 7 14.901028966504699 8 11.996863461701997 9 3.0041846879183947 10-11 6.493410500877084 12-13 3.5507057211170507 14-15 7.385771467785221 16-17 11.43506515337593 18-19 14.50129832118073 20-21 14.954375826901057 22-23 12.8432195596585 24-25 15.371929959873668 26-27 12.909495246740354 28-29 12.941384704530579 30-31 13.7907567046967 32-33 12.28421466895282 34-35 19.127074297986958 36-37 23.902755446917155 38-39 22.966243649301003 40-41 18.089752260937836 42-43 18.003527122354658 44-45 23.347755278817687 46-47 12.747007335069702 48-49 21.48963815106407 50-51 21.178011403076813 52-53 11.204991367597938 54-55 12.796126988270611 56-57 12.307476668782742 58-59 13.331597953537308 60-61 12.752841375409618 62-63 12.70463638107556 64-65 13.76685197160899 66-67 12.1046943259508 68-69 13.130941574558047 70-71 11.762562468728554 72-73 11.132387229977871 74-75 11.205634100855725 76-77 11.245409401308802 78-79 11.64111060351664 80-81 11.634683270938766 82-83 10.139537390265657 84-85 10.052323431285881 86-87 10.189398658764013 88-89 11.59164486317692 90-91 9.087778575426231 92-93 11.124995797513314 94-95 11.204867765048364 96-97 10.75003510312408 98-99 10.605790927770625 100-101 10.731371118138327 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2022612.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.58057317543002 #Duplication Level Percentage of deduplicated Percentage of total 1 98.82044034832464 96.42955210637909 2 0.8591442062307499 1.6767156816869286 3 0.16993710513427568 0.497476803683278 4 0.05599515451345455 0.2185615708987865 5 0.0283572457361729 0.13835581463061353 6 0.016719386401506407 0.09788923849202914 7 0.012993901549654923 0.08875666527003051 8 0.006049470068635564 0.0472248605364053 9 0.004955800681472532 0.043523088393714875 >10 0.023426243787095753 0.36695888549686717 >50 0.0013195829857467671 0.0867966524418762 >100 5.599625206409534E-4 0.09316672534750528 >500 5.079603295378509E-5 0.031227247867210796 >1k 5.0796032953784585E-5 0.18379465887558175 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3708 0.18332730152891408 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.888203965960846E-5 2 0.0 4.944101982980423E-5 0.0 0.0 9.888203965960846E-5 3 0.0 4.944101982980423E-5 0.0 0.0 9.888203965960846E-5 4 0.0 4.944101982980423E-5 0.0 0.0 9.888203965960846E-5 5 0.0 4.944101982980423E-5 0.0 0.0 9.888203965960846E-5 6 0.0 4.944101982980423E-5 0.0 0.0 2.966461189788254E-4 7 0.0 4.944101982980423E-5 0.0 0.0 2.966461189788254E-4 8 0.0 4.944101982980423E-5 0.0 0.0 2.966461189788254E-4 9 0.0 4.944101982980423E-5 0.0 0.0 2.966461189788254E-4 10-11 0.0 4.944101982980423E-5 0.0 0.0 2.966461189788254E-4 12-13 0.0 4.944101982980423E-5 0.0 0.0 4.449691784682381E-4 14-15 0.0 4.944101982980423E-5 0.0 0.0 4.944101982980423E-4 16-17 0.0 4.944101982980423E-5 0.0 0.0 7.910563172768677E-4 18-19 0.0 4.944101982980423E-5 0.0 0.0 9.888203965960846E-4 20-21 0.0 7.416152974470635E-5 0.0 9.888203965960846E-5 0.0011124229461705953 22-23 0.0 9.888203965960846E-5 0.0 1.9776407931921692E-4 0.001236025495745106 24-25 0.0 9.888203965960846E-5 0.0 6.18012747872553E-4 0.00128546651557491 26-27 0.0 9.888203965960846E-5 0.0 0.0010135409065109868 0.00128546651557491 28-29 2.4720509914902116E-5 9.888203965960846E-5 0.0 0.0022248458923411906 0.00128546651557491 30-31 4.944101982980423E-5 9.888203965960846E-5 0.0 0.006773419716683181 0.001310187025489812 32-33 4.944101982980423E-5 9.888203965960846E-5 0.0 0.0186639849857511 0.0014090690651494208 34-35 4.944101982980423E-5 9.888203965960846E-5 0.0 0.036141385495586896 0.001483230594894127 36-37 4.944101982980423E-5 9.888203965960846E-5 0.0 0.06145518764844666 0.001483230594894127 38-39 4.944101982980423E-5 9.888203965960846E-5 0.0 0.0980415423225018 0.001483230594894127 40-41 4.944101982980423E-5 9.888203965960846E-5 0.0 0.13445485342715263 0.001483230594894127 42-43 4.944101982980423E-5 9.888203965960846E-5 0.0 0.1725738797159317 0.001483230594894127 44-45 4.944101982980423E-5 9.888203965960846E-5 0.0 0.22236098668454451 0.001483230594894127 46-47 4.944101982980423E-5 9.888203965960846E-5 0.0 0.2790203954095002 0.001507951104809029 48-49 4.944101982980423E-5 9.888203965960846E-5 0.0 0.33610005280300914 0.0015821126345537354 50-51 4.944101982980423E-5 9.888203965960846E-5 0.0 0.4029195911029896 0.0015821126345537354 52-53 4.944101982980423E-5 9.888203965960846E-5 0.0 0.49107292945953057 0.0017798767138729524 54-55 4.944101982980423E-5 9.888203965960846E-5 0.0 0.5880267693457766 0.0018293177337027565 56-57 4.944101982980423E-5 9.888203965960846E-5 0.0 0.6963026027730479 0.0018540382436176587 58-59 4.944101982980423E-5 9.888203965960846E-5 0.0 0.8106102406195553 0.001928199773362365 60-61 4.944101982980423E-5 9.888203965960846E-5 0.0 0.9292686882110854 0.001928199773362365 62-63 4.944101982980423E-5 9.888203965960846E-5 0.0 1.0494845279272544 0.001977640793192169 64-65 4.944101982980423E-5 9.888203965960846E-5 0.0 1.1766962719493408 0.0020270818130219736 66-67 7.416152974470635E-5 9.888203965960846E-5 0.0 1.316317711948708 0.002076522832851778 68-69 9.888203965960846E-5 9.888203965960846E-5 0.0 1.4636272305316096 0.002125963852681582 70-71 9.888203965960846E-5 9.888203965960846E-5 0.0 1.619860853193791 0.002175404872511386 72-73 9.888203965960846E-5 9.888203965960846E-5 0.0 1.7814588265075062 0.002299007422085897 74-75 9.888203965960846E-5 9.888203965960846E-5 0.0 1.9474817710959886 0.0024226099716604076 76-77 9.888203965960846E-5 9.888203965960846E-5 0.0 2.12393677086856 0.0024226099716604076 78-79 9.888203965960846E-5 9.888203965960846E-5 0.0 2.3208850733605852 0.0024226099716604076 80-81 9.888203965960846E-5 9.888203965960846E-5 0.0 2.524433751999889 0.002472050991490212 82-83 9.888203965960846E-5 1.483230594894127E-4 0.0 2.733173737721323 0.0025956535410647224 84-85 9.888203965960846E-5 1.483230594894127E-4 0.0 2.944830743612715 0.0026698150708094285 86-87 1.2360254957451057E-4 1.483230594894127E-4 0.0 3.167513096926153 0.0026945355807243307 88-89 1.483230594894127E-4 1.483230594894127E-4 0.0 3.4020612949987443 0.002719256090639233 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1495 0.0 50.511284 1 GTATCAA 2560 0.0 48.573048 1 TATCAAC 3380 0.0 36.1825 2 TCAACGC 3495 0.0 34.980366 4 ATCAACG 3560 0.0 34.20828 3 CAACGCA 3605 0.0 33.114742 5 AACGCAG 4045 0.0 29.0934 6 GTACATG 5385 0.0 28.700602 1 GTACTAG 420 0.0 27.568739 1 TACATGG 5480 0.0 26.725225 2 ACATGGG 5965 0.0 24.549824 3 ACGCAGA 4660 0.0 24.291195 7 GTATAAG 450 0.0 23.493359 1 GTGGTAT 640 0.0 22.811634 1 CTAATAC 290 4.9112714E-11 22.558548 3 CGCAGAG 5040 0.0 22.274906 8 CATGGGG 5135 0.0 20.96711 4 AGTACAT 3730 0.0 20.508965 2 GAGTACA 3995 0.0 20.036352 1 GCAGAGT 5320 0.0 19.147547 9 >>END_MODULE