FastQCFastQC Report
Sat 18 Jun 2016
SRR1930949_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930949_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022612
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT20610.10189794186922653No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18800.047.739931
GTATCAA32950.044.100531
TCAACGC42700.033.4655724
CAACGCA42600.033.422775
ATCAACG44200.032.3314633
TATCAAC45100.032.1081432
AACGCAG45200.031.604446
TAGTACG700.00841513527.1282374
GTACATG71600.026.1976221
ACGCAGA54100.025.9665647
TACATGG73500.024.8693982
CGCAGAG58200.024.0557618
ACATGGG75400.023.801353
TAATACA3850.023.4289344
CATGGGG60550.022.3455144
CTAGTAC6050.021.972723
AGTACAT50450.021.362722
GAGTACA53800.020.8308751
GCAGAGT69100.019.7808639
ATGGGGG33600.019.492645
Produced by FastQC (version 0.11.5)