##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930949_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2022612 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.22303585660522 31.0 31.0 33.0 30.0 34.0 2 31.33432165931973 31.0 31.0 34.0 30.0 34.0 3 31.422747912105734 31.0 31.0 34.0 28.0 34.0 4 35.290696386652506 37.0 35.0 37.0 33.0 37.0 5 35.060740764911905 35.0 35.0 37.0 32.0 37.0 6 35.09857253887547 36.0 35.0 37.0 32.0 37.0 7 34.96767101154349 36.0 35.0 37.0 32.0 37.0 8 34.94441000053396 36.0 35.0 37.0 32.0 37.0 9 36.455082833484624 38.0 35.0 39.0 32.0 39.0 10-11 36.37257912046404 38.0 35.0 39.0 32.0 39.0 12-13 36.24715071402721 38.0 35.0 39.0 32.0 39.0 14-15 37.260828077752926 39.0 36.0 40.0 32.0 41.0 16-17 37.20051967455943 39.0 36.0 40.0 31.0 41.0 18-19 37.143157956147796 39.0 36.0 40.0 31.0 41.0 20-21 37.0836628577305 39.0 36.0 40.0 31.0 41.0 22-23 36.93936034197365 39.0 36.0 40.0 31.0 41.0 24-25 36.791130231601514 38.5 35.5 40.0 30.5 41.0 26-27 36.491508999254435 38.0 35.0 40.0 30.0 41.0 28-29 36.282112683994754 38.0 35.0 40.0 30.0 41.0 30-31 36.04147384668933 38.0 34.5 40.0 29.5 41.0 32-33 35.812117697314164 38.0 34.0 40.0 29.0 41.0 34-35 36.07692083306141 38.0 35.0 40.0 30.0 41.0 36-37 36.15119236907523 38.0 35.0 40.0 30.0 41.0 38-39 36.114567450405715 38.0 35.0 40.0 30.0 41.0 40-41 36.00784851469288 38.0 35.0 40.0 29.0 41.0 42-43 35.85763779706637 38.0 34.0 40.0 29.0 41.0 44-45 35.66244687562419 38.0 34.0 40.0 28.0 41.0 46-47 35.42770956565075 38.0 34.0 40.0 27.0 41.0 48-49 35.20725749674183 38.0 33.5 40.0 27.0 41.0 50-51 34.88476361259599 38.0 33.0 40.0 26.0 41.0 52-53 34.61393732460799 37.0 33.0 40.0 26.0 41.0 54-55 34.37202735868273 37.0 33.0 40.0 26.0 41.0 56-57 34.038727645242886 36.5 32.0 39.5 25.5 41.0 58-59 33.70202144553676 36.0 32.0 39.0 25.0 41.0 60-61 33.30045258309552 35.5 31.5 39.0 23.5 40.0 62-63 32.90892618060211 35.0 31.0 38.5 23.0 40.0 64-65 32.52682669735965 35.0 31.0 38.0 22.5 40.0 66-67 32.11457758581477 35.0 31.0 37.5 22.0 40.0 68-69 31.675526250215068 34.5 30.0 37.0 21.5 39.0 70-71 31.228190824537776 34.0 30.0 36.0 20.0 39.0 72-73 30.779739267837826 34.0 29.5 36.0 19.5 38.5 74-75 30.265256509899082 34.0 29.0 35.0 18.0 37.0 76-77 28.961170506256266 32.5 27.0 34.5 16.5 36.0 78-79 29.727444017933248 33.5 29.0 35.0 18.0 36.0 80-81 29.761455237089464 34.0 29.0 35.0 18.0 36.0 82-83 29.536475359584536 34.0 29.0 35.0 16.5 35.5 84-85 29.27747808279591 33.0 29.0 35.0 13.0 35.0 86-87 28.98174538665844 33.0 29.0 35.0 8.5 35.0 88-89 28.66441240336753 33.0 29.0 35.0 7.0 35.0 90-91 28.34505678795538 33.0 27.5 35.0 2.0 35.0 92-93 27.983678777738884 33.0 27.0 35.0 2.0 35.0 94-95 27.546732146353328 33.0 27.0 35.0 2.0 35.0 96-97 27.048084605450775 33.0 25.0 35.0 2.0 35.0 98-99 26.50979624366908 33.0 25.0 34.0 2.0 35.0 100-101 25.212332370222267 31.5 21.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5189388717032273 1101 2 0.5867010089329838 1101 3 0.61270034365916 1101 4 0.4063577363678874 1101 5 0.5596695539047047 1101 6 0.4154752655342193 1101 7 0.5084224963006392 1101 8 0.5512479293170642 1101 9 0.6500462230746109 1101 10-11 0.6593507307008153 1101 12-13 0.6854505832785023 1101 14-15 0.8633692264250712 1101 16-17 0.6965077469958985 1101 18-19 0.731451242276151 1101 20-21 0.7645605060892962 1101 22-23 0.7103836400803516 1101 24-25 0.7012840378436351 1101 26-27 0.6902350054162838 1101 28-29 0.6840556752117308 1101 30-31 0.7153362676100059 1101 32-33 0.5862131653275497 1101 34-35 0.7520978864614207 1101 36-37 0.797095634698394 1101 38-39 0.7899695899343229 1101 40-41 0.7362705902601547 1101 42-43 0.7241791827598476 1101 44-45 0.8144406230609036 1101 46-47 0.8487614219178425 1101 48-49 0.8185619688978178 1101 50-51 0.7249042857732633 1101 52-53 0.7842244640703839 1101 54-55 0.7386121757482087 1101 56-57 0.9611139988205082 1101 58-59 0.8357267992771114 1101 60-61 0.8522207922807965 1101 62-63 0.7329241431726814 1101 64-65 0.7225814591795583 1101 66-67 0.7394652610845966 1101 68-69 0.5358198176022988 1101 70-71 0.6967080954775717 1101 72-73 0.8966240974705499 1101 74-75 0.7500679394034222 1101 76-77 0.831657302610072 1101 78-79 0.6907168030628092 1101 80-81 0.5794071397587395 1101 82-83 0.8818619191026826 1101 84-85 0.8233106714143013 1101 86-87 0.718409386073148 1101 88-89 0.840946292444265 1101 90-91 0.6075685093577867 1101 92-93 0.7877955830036925 1101 94-95 0.8261350151863702 1101 96-97 0.615690299970435 1101 98-99 0.7173123601783367 1101 100-101 0.7078809494015061 1102 1 0.4496927786160718 1102 2 0.5334763384897698 1102 3 0.5910743054768268 1102 4 0.31964089137289164 1102 5 0.446254831880708 1102 6 0.49733156924482813 1102 7 0.5194694768840478 1102 8 0.5300828406023044 1102 9 0.8207955739564099 1102 10-11 0.717596681993129 1102 12-13 0.7092989650739057 1102 14-15 0.9232696446036357 1102 16-17 0.7955363360907128 1102 18-19 0.9249101996423974 1102 20-21 0.8732878624987706 1102 22-23 0.8646299307504819 1102 24-25 0.8472409089660644 1102 26-27 0.9531265974469534 1102 28-29 0.9129820557937336 1102 30-31 1.0291329059099539 1102 32-33 1.074093890294371 1102 34-35 0.9950593996216099 1102 36-37 0.9988517987062124 1102 38-39 1.1044573037597871 1102 40-41 1.1712840375252611 1102 42-43 1.0825537954391038 1102 44-45 1.1512032608169136 1102 46-47 1.2427451364313598 1102 48-49 1.0246661206973329 1102 50-51 1.3311116456230039 1102 52-53 1.066019749508051 1102 54-55 1.1502265681573007 1102 56-57 1.1001842895464193 1102 58-59 1.3737331637735366 1102 60-61 1.2569287297701948 1102 62-63 1.0127753283218794 1102 64-65 0.9723341317799168 1102 66-67 1.065116413225816 1102 68-69 1.0899251270056247 1102 70-71 1.0843373089345967 1102 72-73 0.940457765145279 1102 74-75 1.085668905467962 1102 76-77 1.4706304290004155 1102 78-79 1.4341501908670615 1102 80-81 1.3325896204246632 1102 82-83 1.558622441558569 1102 84-85 1.568303686274863 1102 86-87 1.561170759298097 1102 88-89 1.5009844375817494 1102 90-91 1.3857065338534262 1102 92-93 1.4190737821626875 1102 94-95 1.4285039188797803 1102 96-97 1.4129454074904437 1102 98-99 1.4805855198091145 1102 100-101 1.8637062767500083 1103 1 0.46042151512623875 1103 2 0.5409844839926059 1103 3 0.4732384190576191 1103 4 0.3861877578448514 1103 5 0.3750308774534332 1103 6 0.4995087030934542 1103 7 0.479940337766422 1103 8 0.42571429001153405 1103 9 0.5450134959879307 1103 10-11 0.6587174682596952 1103 12-13 0.6581656465756751 1103 14-15 0.8438433244098178 1103 16-17 0.7725800547293531 1103 18-19 0.8449639418276575 1103 20-21 0.894085815075087 1103 22-23 0.9087786232836592 1103 24-25 0.7694492860846012 1103 26-27 0.8255559564494916 1103 28-29 0.8508389322458143 1103 30-31 0.9593504491537672 1103 32-33 0.961156275197304 1103 34-35 0.9307203371614889 1103 36-37 0.8694774386940196 1103 38-39 0.9351288997410521 1103 40-41 0.905390848495415 1103 42-43 0.976035423115853 1103 44-45 1.0938409888786822 1103 46-47 1.0911414003129636 1103 48-49 1.1523122058189017 1103 50-51 1.127707570923917 1103 52-53 0.9818604858427022 1103 54-55 1.1769619956881812 1103 56-57 1.1227210388941842 1103 58-59 1.1342941098652375 1103 60-61 1.0915445759017928 1103 62-63 0.8991448361799286 1103 64-65 1.0388108150681674 1103 66-67 0.7241967292046141 1103 68-69 0.9752636024791208 1103 70-71 0.8710265476474213 1103 72-73 0.8735800191022882 1103 74-75 0.8638566883554439 1103 76-77 1.0794336165482399 1103 78-79 1.3435843330718455 1103 80-81 1.2585588983236526 1103 82-83 1.2145965640001322 1103 84-85 1.3048558271194182 1103 86-87 1.3806336419083856 1103 88-89 1.2137949617908035 1103 90-91 1.2509319864174309 1103 92-93 1.3745354442348017 1103 94-95 1.4530845995424855 1103 96-97 1.4732567163631636 1103 98-99 1.6478696567081847 1103 100-101 1.7380848701402094 1104 1 0.3425749852051432 1104 2 0.42211981927081155 1104 3 0.403846039015864 1104 4 0.18783346138826573 1104 5 0.34876391939888407 1104 6 0.33712178690309713 1104 7 0.4934066980363454 1104 8 0.5018297761506432 1104 9 0.6561467748484588 1104 10-11 0.5770846945245509 1104 12-13 0.5617288555771296 1104 14-15 0.6904641308452852 1104 16-17 0.5342273394586599 1104 18-19 0.7560267243250252 1104 20-21 0.5845486360727961 1104 22-23 0.5416540843077584 1104 24-25 0.6899358927611061 1104 26-27 0.8458466011944736 1104 28-29 0.5062239542981004 1104 30-31 0.7228698623717307 1104 32-33 0.6643055945537171 1104 34-35 0.7944932876259116 1104 36-37 0.9029689052176622 1104 38-39 0.7199932276395913 1104 40-41 0.7969751507796161 1104 42-43 0.6461567911789245 1104 44-45 0.7792479570028945 1104 46-47 0.8607084116066233 1104 48-49 0.8725789575746816 1104 50-51 0.7944443908524619 1104 52-53 0.8494258734642912 1104 54-55 0.9403135447795208 1104 56-57 0.8525456433294281 1104 58-59 0.7901754972704609 1104 60-61 0.8457606153902688 1104 62-63 0.7944772279557526 1104 64-65 0.6602721724834169 1104 66-67 0.6986540068536371 1104 68-69 0.6941133094450755 1104 70-71 0.6674493006935549 1104 72-73 0.7128760146942419 1104 74-75 0.9171788979072559 1104 76-77 1.0764363713595237 1104 78-79 1.1676929504693128 1104 80-81 1.031833045956045 1104 82-83 1.083867201487422 1104 84-85 1.0929544685916461 1104 86-87 1.1008846256053069 1104 88-89 1.1018979175791372 1104 90-91 1.0025945858739114 1104 92-93 1.0561153730726751 1104 94-95 1.0580124902383012 1104 96-97 1.0288286118130507 1104 98-99 1.175793793948987 1104 100-101 1.1844316408527042 1105 1 0.24741106603249463 1105 2 0.3562940829632346 1105 3 0.3130057188827955 1105 4 0.20027364284801763 1105 5 0.24419870111483988 1105 6 0.32768763500955345 1105 7 0.3215138093452481 1105 8 0.32332440050609534 1105 9 0.41988166932294746 1105 10-11 0.24871205924944206 1105 12-13 0.3081812414131804 1105 14-15 0.49863208896490363 1105 16-17 0.33859042667783257 1105 18-19 0.4725580065496615 1105 20-21 0.37326731488951737 1105 22-23 0.4551357421888227 1105 24-25 0.4724580397509399 1105 26-27 0.4891063450162534 1105 28-29 0.4465305572101954 1105 30-31 0.5324766426672412 1105 32-33 0.6033810826822972 1105 34-35 0.6116441041580316 1105 36-37 0.557206125215238 1105 38-39 0.3075655604962009 1105 40-41 0.306955372107943 1105 42-43 0.25699548596382016 1105 44-45 0.3812709751317982 1105 46-47 0.3703232538019563 1105 48-49 0.3010151154331666 1105 50-51 0.41700882715383614 1105 52-53 0.3651183722879665 1105 54-55 0.5139431136667199 1105 56-57 0.45317630867823766 1105 58-59 0.42709022333554003 1105 60-61 0.5408294127904014 1105 62-63 0.37556524293661653 1105 64-65 0.48141341329409926 1105 66-67 0.5162181848716187 1105 68-69 0.6186755407195896 1105 70-71 0.5066129942194593 1105 72-73 0.5466895678069221 1105 74-75 0.7197692317109734 1105 76-77 0.7785096196632288 1105 78-79 0.7150342418127558 1105 80-81 0.6172821596982132 1105 82-83 0.7414904194131111 1105 84-85 0.829846802892046 1105 86-87 0.7995281082941581 1105 88-89 0.8671436077588126 1105 90-91 0.7196732343536816 1105 92-93 0.7254524449949784 1105 94-95 0.7018096580258906 1105 96-97 0.4443230429422691 1105 98-99 0.6803859428689201 1105 100-101 1.1954796856690848 1106 1 0.03808421146900187 1106 2 0.09942182530343402 1106 3 0.04682420707134227 1106 4 0.035947802545543084 1106 5 0.07568221553780319 1106 6 0.006993002581154428 1106 7 0.02994189227259625 1106 8 0.07086673524973719 1106 9 -0.01691090492266767 1106 10-11 0.06185705255020224 1106 12-13 0.053042922631789224 1106 14-15 0.13840173238823184 1106 16-17 0.12309863366890283 1106 18-19 0.0669095315209347 1106 20-21 -0.05157242760751046 1106 22-23 0.11653567383275032 1106 24-25 0.11167910859454366 1106 26-27 0.15085817835697668 1106 28-29 0.043710506718753095 1106 30-31 0.05131622151879611 1106 32-33 0.17249721812620322 1106 34-35 0.07442903479622487 1106 36-37 0.17116282564514762 1106 38-39 0.158148717967471 1106 40-41 0.15459610721705985 1106 42-43 0.2320606458420471 1106 44-45 0.10243751593917949 1106 46-47 0.011412182333202736 1106 48-49 0.201964536196364 1106 50-51 0.10476090084885215 1106 52-53 -0.02151451977079688 1106 54-55 0.11720980124363933 1106 56-57 0.2968668593452577 1106 58-59 0.3435818092306562 1106 60-61 0.1883265106401737 1106 62-63 0.11423332644353934 1106 64-65 0.22220270866394998 1106 66-67 0.14647444345875016 1106 68-69 0.2853286412899543 1106 70-71 0.2826036459716583 1106 72-73 0.060616905806423205 1106 74-75 0.18067745983958616 1106 76-77 0.13997871709024778 1106 78-79 0.30482596032890186 1106 80-81 0.12811855854315013 1106 82-83 0.36121413130161173 1106 84-85 0.3990041693249502 1106 86-87 0.3132439643475067 1106 88-89 0.42655298822431575 1106 90-91 0.34406979166199037 1106 92-93 0.39856357561475164 1106 94-95 0.31844903624126886 1106 96-97 0.45781399300653547 1106 98-99 0.4530911133841009 1106 100-101 0.5893753045728651 1107 1 0.05110540934921559 1107 2 0.10620781337129515 1107 3 0.06460367912413645 1107 4 -0.061011251549047074 1107 5 0.006397477344954439 1107 6 0.15914820372771032 1107 7 0.02810207625419281 1107 8 -0.040996245118897434 1107 9 -0.05669650969552009 1107 10-11 -0.05829593215132434 1107 12-13 0.06861282397498769 1107 14-15 -0.14594774933026144 1107 16-17 -0.018617403060758875 1107 18-19 -0.06417215197738102 1107 20-21 0.010547402076184653 1107 22-23 0.033922060194115033 1107 24-25 0.03463868763663669 1107 26-27 0.0429960479033582 1107 28-29 0.10564868790063286 1107 30-31 -0.04542181301108883 1107 32-33 0.12337504700997926 1107 34-35 -0.021113494284207945 1107 36-37 0.06847267799324186 1107 38-39 0.14309189031690295 1107 40-41 -0.03225229127642848 1107 42-43 0.14788364687527888 1107 44-45 0.1343961950712611 1107 46-47 0.022763755509217276 1107 48-49 0.08428734558209783 1107 50-51 -0.057357054022325826 1107 52-53 0.048196842426321496 1107 54-55 0.13201248764166706 1107 56-57 0.019987255638966417 1107 58-59 0.03105681173040864 1107 60-61 -0.0475413609060098 1107 62-63 -0.11864317757272147 1107 64-65 -0.15134514488402573 1107 66-67 0.029321992037225186 1107 68-69 -0.01269363981526439 1107 70-71 -0.0018962373733373283 1107 72-73 -0.035905941908804806 1107 74-75 0.1273950797442609 1107 76-77 0.10739468382690376 1107 78-79 0.260538305761024 1107 80-81 0.11133190387528202 1107 82-83 0.2537948732274202 1107 84-85 0.11148396301225105 1107 86-87 0.021031310612880105 1107 88-89 0.21659335918722178 1107 90-91 0.07026133917389998 1107 92-93 0.26533981465751566 1107 94-95 0.31144348679621103 1107 96-97 0.45985295577692753 1107 98-99 0.11354215161361125 1107 100-101 0.43507177659233065 1108 1 0.2823920012580494 1108 2 0.371009508495721 1108 3 0.31948416908705113 1108 4 0.11609829356606838 1108 5 0.3332172155879576 1108 6 0.19846781123863622 1108 7 0.23510387721930925 1108 8 0.35721136578897017 1108 9 0.4141073566272695 1108 10-11 0.33413938684540767 1108 12-13 0.49174717262594214 1108 14-15 0.4423347867465637 1108 16-17 0.47485697449766917 1108 18-19 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-1.1726914995350768 2316 48-49 -1.1305430802072394 2316 50-51 -0.5897537288847587 2316 52-53 -0.9473364674905511 2316 54-55 -1.3702218330858003 2316 56-57 -1.1664058286993253 2316 58-59 -1.0049362413859342 2316 60-61 -0.6430493029892901 2316 62-63 -1.1785873683388317 2316 64-65 -0.8102765736784718 2316 66-67 -0.49409837247903 2316 68-69 -0.5351168533343689 2316 70-71 -0.35526625649678323 2316 72-73 -0.48475658391013 2316 74-75 -0.029389840054356853 2316 76-77 -0.513125942173172 2316 78-79 -0.3115882992422918 2316 80-81 -0.30915554880395035 2316 82-83 -0.6503108130700497 2316 84-85 -0.7954297473261178 2316 86-87 -0.47330190563814156 2316 88-89 -0.6650891135911436 2316 90-91 -0.44051803872876505 2316 92-93 -0.528113332905221 2316 94-95 -0.29395010489874807 2316 96-97 -0.17641719213705898 2316 98-99 -0.20190685904088213 2316 100-101 -0.07803363651308359 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 21.0 4 45.0 5 98.0 6 171.0 7 466.0 8 1117.0 9 1651.0 10 2672.0 11 3751.0 12 5127.0 13 6680.0 14 7900.0 15 9693.0 16 11347.0 17 12861.0 18 12882.0 19 13057.0 20 13548.0 21 14580.0 22 16280.0 23 18727.0 24 23357.0 25 28606.0 26 36123.0 27 45629.0 28 56238.0 29 69902.0 30 83313.0 31 98726.0 32 117794.0 33 140928.0 34 172932.0 35 215288.0 36 277879.0 37 320260.0 38 172962.0 39 9995.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.91232206630067 17.822248771413115 13.123355167185727 26.14207399510049 2 17.470040354917593 19.712156016600794 36.50176954484281 26.316034083638804 3 20.49266690498051 23.030857687574503 27.841188048906744 28.635287358538235 4 14.98113448301955 15.827696428633251 33.59669387317051 35.59447521517669 5 15.51592179074783 36.13702143901781 32.74510598870548 15.601950781528885 6 35.37712621105778 33.44057090534418 16.028234777604407 15.154068105993638 7 30.444692308757194 30.069978819467103 19.699675469145834 19.785653402629865 8 27.853092931318514 33.72638944098028 18.76163099991496 19.65888662778625 9 27.458949121235314 14.499271239367708 17.88528892343168 40.1564907159653 10-11 27.154689085202698 24.22088368901203 26.18601590418726 22.43841132159801 12-13 27.41096661149049 21.296620409648515 26.260770726169923 25.03164225269107 14-15 24.607870717602147 22.727751406474106 23.476511301481107 29.18786657444264 16-17 25.126470128724637 25.5368058728021 24.13238426351668 25.204339734956584 18-19 25.117199937506552 24.97755377699727 25.340104775409223 24.565141510086956 20-21 25.770711337616902 24.14311296481975 25.269354676032773 24.816821021530576 22-23 25.652578362181767 23.9376914449463 25.12516615276834 25.284564040103596 24-25 25.51811444535334 24.13041935905717 25.13202694454683 25.219439251042658 26-27 25.390554892757784 24.341790720820182 25.04218640532273 25.225467981099303 28-29 25.34619855226734 24.547552643587917 24.992348998398604 25.113899805746136 30-31 25.606049855584395 24.33811471812937 25.00334369776631 25.052491728519925 32-33 25.527930805977828 24.374647650508866 24.809335654306825 25.288085889206485 34-35 25.535584043118686 24.35036406116722 25.148702607147715 24.965349288566376 36-37 25.477708266021025 24.327653596275486 24.95926652804967 25.23537160965381 38-39 25.452468469412754 24.26088308634507 24.987621128157105 25.299027316085066 40-41 25.540920709790022 24.079537420212898 25.138857987609825 25.24068388238726 42-43 25.412554961797145 24.13119876979521 25.005173238595695 25.451073029811944 44-45 25.309867884472897 24.156282935244626 25.077128579442252 25.456720600840228 46-47 25.440588398899845 24.177211903952532 24.8639080317686 25.518291665379028 48-49 25.471598987639197 24.243479843146872 24.840196172718425 25.444724996495506 50-51 25.271432445585347 24.22153949791282 24.980874225588785 25.526153830913046 52-53 25.443496916142877 24.165013871606575 24.96720998053251 25.42427923171803 54-55 25.16891131754042 24.097091555546765 24.97852602220832 25.755471104704498 56-57 25.31940090388552 24.267002244879794 24.756623384329355 25.65697346690533 58-59 25.33660738526024 24.38235691188099 24.789965002821237 25.491070700037533 60-61 25.44677683047783 24.484463538845482 24.775974692498522 25.292784938178166 62-63 25.2555775477274 24.495680513651795 24.64431944180849 25.60442249681232 64-65 25.084846086785884 24.34193709152935 24.797130315423292 25.776086506261475 66-67 25.203322851570668 24.457787169322742 24.628809743758147 25.710080235348443 68-69 25.081649975377562 24.42105106866319 24.772966298196252 25.724332657762993 70-71 25.018668801846445 24.202833501010268 24.811518094015543 25.966979603127747 72-73 25.126528528197202 24.341320646383345 24.672685744423628 25.859465080995818 74-75 25.093441038729342 24.445077681690908 24.590380850905667 25.871100428674083 76-77 25.079485570626446 24.26520625591416 24.530021808485454 26.12528636497394 78-79 25.081305506227675 24.29516068143594 24.64601974472234 25.977514067614045 80-81 25.051375768040852 24.51723588108855 24.561461190375248 25.869927160495354 82-83 25.217318002661905 24.355338542439185 24.672848771786185 25.75449468311273 84-85 25.139009641742867 24.348249775105106 24.38644297236518 26.126297610786846 86-87 24.94986680589258 24.447941572580405 24.61967495495923 25.982516666567783 88-89 24.92106109511554 24.29773235898099 24.625897695970327 26.155308849933146 90-91 24.94062356688764 24.26256905206609 24.568209040644458 26.22859834040181 92-93 25.069873012105386 24.194976857346308 24.60902266793259 26.126127462615713 94-95 24.98414771045969 24.138420338197008 24.6942702100314 26.183161741311896 96-97 24.808243004590107 24.132507866066256 24.723525817111735 26.335723312231906 98-99 24.73272184680008 24.07090435535832 24.6460022980186 26.550371499823 100-101 24.773189321530772 24.14640079263843 24.574906111503346 26.505503774327455 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 92.0 1 74.0 2 74.0 3 100.0 4 85.5 5 85.5 6 143.5 7 277.0 8 448.5 9 508.5 10 445.5 11 382.5 12 397.0 13 439.0 14 496.5 15 569.5 16 642.5 17 714.5 18 810.0 19 883.5 20 949.5 21 1102.5 22 1280.0 23 1461.5 24 1686.5 25 1977.0 26 2358.5 27 2813.0 28 3272.5 29 3879.5 30 4664.0 31 5426.0 32 6390.5 33 7145.0 34 7921.0 35 8816.5 36 9982.5 37 11975.0 38 14339.5 39 17383.5 40 21982.0 41 28248.5 42 36260.0 43 46014.5 44 56660.0 45 67679.0 46 78831.5 47 88456.5 48 97142.5 49 103954.5 50 107359.0 51 110291.0 52 112805.5 53 113673.0 54 112725.0 55 107693.5 56 99249.5 57 89068.5 58 79752.0 59 71070.5 60 60968.5 61 50742.0 62 40598.0 63 31600.0 64 24078.0 65 17580.0 66 12561.5 67 9681.5 68 7299.5 69 5079.5 70 3620.5 71 2497.5 72 1700.5 73 1155.5 74 779.0 75 525.5 76 351.0 77 194.5 78 97.0 79 55.0 80 31.0 81 20.0 82 12.0 83 7.5 84 5.5 85 1.0 86 0.5 87 2.0 88 2.5 89 1.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018293177337027566 2 0.0028675791501286455 3 0.003411430368256492 4 0.03307604226613903 5 0.001878758753532561 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 4.944101982980423E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.4720509914902116E-5 24-25 6.674537677023571E-4 26-27 0.0020270818130219736 28-29 4.944101982980423E-5 30-31 0.0074408734843855375 32-33 0.0233361613596676 34-35 0.014189572691153816 36-37 6.180127478725529E-4 38-39 6.180127478725529E-4 40-41 0.002076522832851778 42-43 0.00912186815859888 44-45 0.008775781019790251 46-47 0.008627457960300839 48-49 0.01040733467417379 50-51 0.18488469365355295 52-53 0.05124561705359209 54-55 0.01962808487243228 56-57 0.011470316600514582 58-59 0.0221990179035821 60-61 0.05324797835669916 62-63 0.09608862203922452 64-65 0.10093384198254535 66-67 0.10474080050944026 68-69 0.10367781858309948 70-71 0.09200973790326568 72-73 0.07057705580704554 74-75 0.23543813642952777 76-77 0.28361841025367196 78-79 0.0072925504248961244 80-81 7.168947875321614E-4 82-83 0.0 84-85 2.4720509914902116E-5 86-87 0.0 88-89 1.7304356940431483E-4 90-91 0.006649817167108669 92-93 0.018392059376687177 94-95 0.0016562741642984417 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2022612.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.49618913838719 #Duplication Level Percentage of deduplicated Percentage of total 1 78.18986463266059 45.73809110257005 2 11.794803491719346 13.79902111803449 3 3.9208027146546454 6.880560515122203 4 1.8038954961220544 4.220840485081619 5 1.0540905022156575 3.0830138693292324 6 0.6782031338648159 2.380337927568192 7 0.4700730209145847 1.924823623819081 8 0.33895919511474565 1.586225695010212 9 0.2735808208339077 1.4403091896121938 >10 1.3959371459912981 14.397432814437291 >50 0.06338035187216962 2.4877472644997414 >100 0.015559847880057669 1.4882946417640643 >500 4.248230781402066E-4 0.16679676493946807 >1k 4.248230781402063E-4 0.40650498821213 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 2061 0.10189794186922653 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.483230594894127E-4 2 0.0 0.0 0.0 0.0 1.483230594894127E-4 3 0.0 0.0 0.0 0.0 1.483230594894127E-4 4 0.0 0.0 0.0 0.0 1.483230594894127E-4 5 0.0 0.0 0.0 0.0 1.483230594894127E-4 6 0.0 0.0 0.0 0.0 1.483230594894127E-4 7 0.0 0.0 0.0 0.0 1.483230594894127E-4 8 0.0 0.0 0.0 0.0 1.483230594894127E-4 9 0.0 0.0 0.0 0.0 1.483230594894127E-4 10-11 0.0 0.0 0.0 0.0 1.483230594894127E-4 12-13 0.0 0.0 0.0 0.0 7.168947875321614E-4 14-15 0.0 4.944101982980423E-5 0.0 0.0 0.001038261416425889 16-17 0.0 9.888203965960846E-5 0.0 0.0 0.0012360254957451057 18-19 0.0 9.888203965960846E-5 0.0 0.0 0.0013843485552345186 20-21 0.0 9.888203965960846E-5 0.0 9.888203965960846E-5 0.0018045972237878544 22-23 0.0 9.888203965960846E-5 0.0 2.966461189788254E-4 0.0020518023229368753 24-25 0.0 1.483230594894127E-4 0.0 9.888203965960846E-4 0.002125963852681582 26-27 0.0 1.9776407931921692E-4 0.0 0.001878758753532561 0.002274286912170995 28-29 0.0 1.9776407931921692E-4 0.0 0.004375530254937675 0.002274286912170995 30-31 0.0 1.9776407931921692E-4 0.0 0.014362616260558129 0.002299007422085897 32-33 0.0 1.9776407931921692E-4 0.0 0.04157989767686536 0.0024720509914902115 34-35 0.0 1.9776407931921692E-4 0.0 0.08251706209594327 0.00257093303114982 36-37 0.0 2.2248458923411902E-4 0.0 0.14293398832796403 0.00257093303114982 38-39 0.0 2.4720509914902115E-4 0.0 0.22940633201029165 0.0026698150708094285 40-41 0.0 2.4720509914902115E-4 0.0 0.31731246526768353 0.002719256090639233 42-43 0.0 2.4720509914902115E-4 0.0 0.4070973572786081 0.002743976600554135 44-45 0.0 2.4720509914902115E-4 0.0 0.5192543107625189 0.002793417620383939 46-47 0.0 2.4720509914902115E-4 0.0 0.647306552121712 0.0028428586402137433 48-49 0.0 2.4720509914902115E-4 0.0 0.7761992908180115 0.0030900637393627647 50-51 0.0 2.4720509914902115E-4 0.0 0.9070696703075034 0.0032631073087670795 52-53 0.0 2.4720509914902115E-4 0.0 1.0380142113267399 0.0032631073087670795 54-55 0.0 2.4720509914902115E-4 0.0 1.1840877044138964 0.003361989348426688 56-57 0.0 2.4720509914902115E-4 0.0 1.3466744981242078 0.0035103124079161004 58-59 0.0 2.4720509914902115E-4 0.0 1.5170976934775429 0.003658635467405513 60-61 0.0 2.4720509914902115E-4 0.0 1.6973349312670942 0.0037080764872353175 62-63 0.0 2.4720509914902115E-4 0.0 1.8825904325693705 0.003757517507065122 64-65 0.0 2.4720509914902115E-4 0.0 2.077486932738459 0.003782238016980024 66-67 0.0 2.4720509914902115E-4 0.0 2.2936677919442783 0.0039058405665545345 68-69 0.0 2.4720509914902115E-4 0.0 2.5282159900168697 0.004004722606214143 70-71 0.0 2.4720509914902115E-4 0.0 2.783776621517127 0.004054163626043947 72-73 0.0 2.4720509914902115E-4 0.0 3.0481871955669204 0.004153045665703556 74-75 0.0 2.4720509914902115E-4 0.0 3.3226342966421636 0.004326089235107871 76-77 0.0 2.4720509914902115E-4 0.0 3.6166353210600946 0.004400250764852577 78-79 0.0 2.4720509914902115E-4 0.0 3.950164440831954 0.004548573824341989 80-81 0.0 2.4720509914902115E-4 0.0 4.301368725192969 0.004696896883831402 82-83 0.0 2.4720509914902115E-4 0.0 4.665996246437775 0.004993543002810227 84-85 0.0 2.4720509914902115E-4 0.0 5.052056449778801 0.004993543002810227 86-87 0.0 2.4720509914902115E-4 0.0 5.465778903714603 0.0050677045325549334 88-89 0.0 2.4720509914902115E-4 0.0 5.90590286223952 0.00514186606229964 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1880 0.0 47.73993 1 GTATCAA 3295 0.0 44.10053 1 TCAACGC 4270 0.0 33.465572 4 CAACGCA 4260 0.0 33.42277 5 ATCAACG 4420 0.0 32.331463 3 TATCAAC 4510 0.0 32.108143 2 AACGCAG 4520 0.0 31.60444 6 TAGTACG 70 0.008415135 27.128237 4 GTACATG 7160 0.0 26.197622 1 ACGCAGA 5410 0.0 25.966564 7 TACATGG 7350 0.0 24.869398 2 CGCAGAG 5820 0.0 24.055761 8 ACATGGG 7540 0.0 23.80135 3 TAATACA 385 0.0 23.428934 4 CATGGGG 6055 0.0 22.345514 4 CTAGTAC 605 0.0 21.97272 3 AGTACAT 5045 0.0 21.36272 2 GAGTACA 5380 0.0 20.830875 1 GCAGAGT 6910 0.0 19.780863 9 ATGGGGG 3360 0.0 19.49264 5 >>END_MODULE