FastQCFastQC Report
Sat 18 Jun 2016
SRR1930948_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930948_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3973916
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN72670.18286747882944682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37300.055.8068351
GTATCAA76750.050.3033181
ATCAACG95850.039.88843
TCAACGC99150.038.548444
TATCAAC99400.038.4215662
CAACGCA100350.037.8657655
AACGCAG109050.034.7114646
GTACATG120300.030.9198191
TACATGG122900.029.6400322
ACGCAGA125700.029.4742077
CGCAGAG129150.028.8760388
ACATGGG131450.027.7060533
GCAGAGT134900.025.5374939
CATGGGG92700.023.7388364
CATGGGA50450.021.3604454
GTGGTAT17200.021.0984361
GAGTACA85550.020.9148391
AGAGTAC126700.020.26970510-11
GAGTACT77500.020.18278912-13
CAGAGTA130900.019.87281810-11
Produced by FastQC (version 0.11.5)