##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930948_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3973916 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.143463274009818 28.0 10.0 31.0 2.0 33.0 2 21.13348319390747 28.0 10.0 31.0 2.0 33.0 3 21.551690574234584 28.0 10.0 31.0 2.0 33.0 4 23.458405260705057 32.0 2.0 35.0 2.0 37.0 5 23.442229277116073 32.0 10.0 35.0 2.0 37.0 6 24.106313268826014 32.0 10.0 35.0 2.0 37.0 7 24.145714957236137 32.0 10.0 35.0 2.0 37.0 8 24.801861438440067 32.0 11.0 35.0 2.0 37.0 9 25.826859450476558 32.0 16.0 37.0 2.0 39.0 10-11 25.97357367392768 32.0 15.5 37.0 2.0 39.0 12-13 25.843225171342326 32.0 11.0 37.0 2.0 39.0 14-15 26.008868456202897 32.5 10.5 38.0 2.0 40.0 16-17 25.173351802101507 32.0 10.0 38.0 2.0 40.0 18-19 24.618714260693984 32.0 10.0 38.0 2.0 40.0 20-21 24.105060725994207 31.0 2.0 37.0 2.0 40.0 22-23 24.00739522425738 31.0 8.0 37.0 2.0 39.0 24-25 23.668594278288722 30.0 2.0 37.0 2.0 39.0 26-27 23.286169612040112 30.0 2.0 37.0 2.0 39.0 28-29 23.12730867990164 30.0 2.0 37.0 2.0 39.0 30-31 22.974386474198248 30.0 2.0 37.0 2.0 39.0 32-33 22.593794508993145 30.0 2.0 36.5 2.0 39.0 34-35 19.844808747844695 23.5 2.0 35.0 2.0 38.0 36-37 18.28261556107376 17.0 2.0 34.0 2.0 38.0 38-39 17.370924045702022 15.0 2.0 33.0 2.0 38.0 40-41 18.460757851952582 17.0 2.0 33.5 2.0 38.0 42-43 18.329093393015857 16.5 2.0 33.5 2.0 38.0 44-45 16.504394028459586 12.0 2.0 32.0 2.0 37.5 46-47 17.159758157947977 15.0 2.0 31.5 2.0 37.0 48-49 16.543583206086893 9.0 2.0 33.0 2.0 37.0 50-51 14.823670027247681 7.0 2.0 30.0 2.0 36.5 52-53 17.416519121189275 16.5 2.0 32.0 2.0 37.0 54-55 18.802928521891253 22.5 2.0 34.0 2.0 38.0 56-57 18.942377619456476 23.0 2.0 35.0 2.0 38.0 58-59 18.571341216069992 21.5 2.0 34.0 2.0 38.0 60-61 18.20682407479172 20.0 2.0 34.0 2.0 38.0 62-63 17.713208457350383 17.5 2.0 34.0 2.0 38.0 64-65 17.205072653775268 13.5 2.0 33.0 2.0 37.0 66-67 16.76520326549429 9.5 2.0 32.5 2.0 36.5 68-69 16.294184879599868 7.5 2.0 32.0 2.0 36.0 70-71 15.726344618255645 4.0 2.0 31.5 2.0 36.0 72-73 15.237600769618684 2.0 2.0 31.0 2.0 35.0 74-75 14.70388251789922 2.0 2.0 31.0 2.0 35.0 76-77 14.149970457352396 2.0 2.0 30.0 2.0 34.0 78-79 13.647331121241617 2.0 2.0 29.5 2.0 34.0 80-81 13.1378458930687 2.0 2.0 29.0 2.0 34.0 82-83 12.660627703252914 2.0 2.0 29.0 2.0 34.0 84-85 12.148963516088413 2.0 2.0 27.5 2.0 34.0 86-87 11.609705263020155 2.0 2.0 26.5 2.0 33.0 88-89 11.084683470913829 2.0 2.0 26.0 2.0 33.0 90-91 10.535315416833168 2.0 2.0 25.0 2.0 32.5 92-93 9.947946307873643 2.0 2.0 23.0 2.0 32.0 94-95 9.33621520938037 2.0 2.0 19.0 2.0 32.0 96-97 8.683753506616647 2.0 2.0 6.0 2.0 31.5 98-99 7.9768768388662465 2.0 2.0 2.0 2.0 31.0 100-101 7.114175161226357 2.0 2.0 2.0 2.0 30.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.132352882207872 1101 2 8.23382223828379 1101 3 7.836381659790472 1101 4 9.756899059812046 1101 5 9.559596445928076 1101 6 9.018105610903593 1101 7 8.91010339304362 1101 8 8.007790130250115 1101 9 8.43165872894578 1101 10-11 7.803458373342419 1101 12-13 7.614752506437799 1101 14-15 8.153690792517548 1101 16-17 8.690019980761235 1101 18-19 9.208190374083738 1101 20-21 9.650427387963415 1101 22-23 9.490457871460762 1101 24-25 9.376664501622372 1101 26-27 9.395218124645655 1101 28-29 9.305060527074474 1101 30-31 9.06566436936156 1101 32-33 9.190700960668547 1101 34-35 11.638324493281125 1101 36-37 5.988451920325442 1101 38-39 9.14682152933673 1101 40-41 10.67971307142913 1101 42-43 11.137444314790912 1101 44-45 5.674676143109075 1101 46-47 7.214521264344565 1101 48-49 9.958526154377449 1101 50-51 -2.1862530841027503 1101 52-53 6.93229622830329 1101 54-55 9.46986301007274 1101 56-57 9.945757851698747 1101 58-59 10.16160667561196 1101 60-61 9.918197653050942 1101 62-63 10.07447149363719 1101 64-65 10.167102490277916 1101 66-67 9.913894498891967 1101 68-69 9.823426393903897 1101 70-71 9.88605742697266 1101 72-73 9.804442704473502 1101 74-75 9.70468784757282 1101 76-77 9.459818542518532 1101 78-79 9.460598406453565 1101 80-81 9.24073089086162 1101 82-83 8.943170446444652 1101 84-85 8.82789836588011 1101 86-87 8.637241602215498 1101 88-89 8.337723517122015 1101 90-91 8.196990165393249 1101 92-93 8.063348987931482 1101 94-95 7.629743385090995 1101 96-97 7.054866588526275 1101 98-99 6.556993676658673 1101 100-101 5.940221413703906 1102 1 6.875794903087812 1102 2 7.779508790607924 1102 3 7.573829149226217 1102 4 9.437548699384504 1102 5 9.399123415851019 1102 6 8.739861141602074 1102 7 8.576837857373725 1102 8 7.671560948047784 1102 9 7.756962171650233 1102 10-11 7.363322529444826 1102 12-13 7.108059180561586 1102 14-15 7.442668918294334 1102 16-17 8.164620496545474 1102 18-19 8.41066181952247 1102 20-21 8.7255764993231 1102 22-23 8.601129820255196 1102 24-25 8.527511879201299 1102 26-27 8.804998636701331 1102 28-29 8.74852519896113 1102 30-31 8.48824521698771 1102 32-33 8.558229794871878 1102 34-35 10.595895864148634 1102 36-37 1.772083010308048 1102 38-39 4.102332031449581 1102 40-41 8.449197380402534 1102 42-43 9.52847948754756 1102 44-45 1.79723216473473 1102 46-47 2.528731306601589 1102 48-49 7.665800265737133 1102 50-51 -3.636056744306579 1102 52-53 5.35053818082628 1102 54-55 8.088031882804529 1102 56-57 8.517965843247342 1102 58-59 8.727783720106121 1102 60-61 8.69838575894207 1102 62-63 8.816262072806968 1102 64-65 8.623520058013437 1102 66-67 8.456956672642896 1102 68-69 8.48145842845523 1102 70-71 8.414191965873977 1102 72-73 8.389085104423785 1102 74-75 8.068012636287712 1102 76-77 7.800792846520521 1102 78-79 7.825936866557962 1102 80-81 7.6634655726910985 1102 82-83 7.4996705085873305 1102 84-85 7.367650944801312 1102 86-87 7.2677409807887 1102 88-89 7.058858522093431 1102 90-91 6.94838567247751 1102 92-93 6.829972900434589 1102 94-95 6.574000438285127 1102 96-97 6.207427239781559 1102 98-99 5.828393844443908 1102 100-101 5.11244960162337 1103 1 6.847693367739996 1103 2 7.8597275840415115 1103 3 7.535249364547706 1103 4 9.237097931808119 1103 5 9.08013641650189 1103 6 8.356086238662296 1103 7 8.262207733406541 1103 8 7.525960802148106 1103 9 7.782654192408263 1103 10-11 7.318816637197248 1103 12-13 7.23814179040053 1103 14-15 7.531786427632799 1103 16-17 8.090363224998612 1103 18-19 8.546776261773783 1103 20-21 8.915655870062484 1103 22-23 8.880069077934522 1103 24-25 8.88136631623739 1103 26-27 8.777131317387195 1103 28-29 8.671939573175507 1103 30-31 8.38508602213253 1103 32-33 8.317439803238432 1103 34-35 10.48754353447779 1103 36-37 -2.3938728099344857 1103 38-39 -1.270849510388624 1103 40-41 6.825655874273664 1103 42-43 8.992573544805627 1103 44-45 1.3372435168216796 1103 46-47 1.4207895331037612 1103 48-49 7.656394594789418 1103 50-51 -3.8972654063195247 1103 52-53 5.070090919328301 1103 54-55 7.974753336213002 1103 56-57 8.646788136151912 1103 58-59 8.64983043725983 1103 60-61 8.735055303860161 1103 62-63 8.605043097364511 1103 64-65 8.56195284584437 1103 66-67 8.523188822089612 1103 68-69 8.376116376738619 1103 70-71 8.350404288179133 1103 72-73 8.368964712810921 1103 74-75 8.189624397352127 1103 76-77 7.936546166353745 1103 78-79 7.7245653632951505 1103 80-81 7.487981319993544 1103 82-83 7.270071746101346 1103 84-85 7.065393522819782 1103 86-87 6.931555972004611 1103 88-89 6.729063450913088 1103 90-91 6.395360015810116 1103 92-93 6.191379394993769 1103 94-95 5.891563946640774 1103 96-97 5.555278309958352 1103 98-99 5.015245209272698 1103 100-101 4.405268986043289 1104 1 6.60945667282159 1104 2 7.734675427212789 1104 3 7.071064271102145 1104 4 8.969521803494356 1104 5 8.877826289490056 1104 6 8.12843352907447 1104 7 7.914563982694645 1104 8 7.187513355280203 1104 9 7.318707766250238 1104 10-11 6.808090743980198 1104 12-13 6.438105996209163 1104 14-15 6.846979601181811 1104 16-17 7.534495161266648 1104 18-19 8.08449557865654 1104 20-21 8.161062616603605 1104 22-23 8.023039399739943 1104 24-25 8.0510604101663 1104 26-27 8.087948569892344 1104 28-29 7.916838354992649 1104 30-31 7.574324718338698 1104 32-33 7.542138385457957 1104 34-35 9.801391430705916 1104 36-37 -2.792331623597539 1104 38-39 -1.7379332841410058 1104 40-41 5.96028632654345 1104 42-43 8.008980415873943 1104 44-45 0.7031765943726143 1104 46-47 0.4252300489414367 1104 48-49 6.697676274714393 1104 50-51 -4.50298849324836 1104 52-53 3.827122190998839 1104 54-55 6.365870681552881 1104 56-57 6.931855023780578 1104 58-59 7.191671958403781 1104 60-61 7.129190131991976 1104 62-63 7.1754095201113195 1104 64-65 7.214766599784536 1104 66-67 7.019514384571867 1104 68-69 7.128629612917134 1104 70-71 7.0333730106068355 1104 72-73 6.807828233956052 1104 74-75 6.860609177296048 1104 76-77 6.665015443842238 1104 78-79 6.397984236778475 1104 80-81 6.217569188093869 1104 82-83 6.004934285193146 1104 84-85 5.943618732907787 1104 86-87 5.698617053479039 1104 88-89 5.620526314955953 1104 90-91 5.37315758416581 1104 92-93 5.326304463262412 1104 94-95 4.866866279194484 1104 96-97 4.439819055433617 1104 98-99 4.143015186887312 1104 100-101 3.8622166002261693 1105 1 5.948314082967244 1105 2 6.869689822526787 1105 3 6.720523211760099 1105 4 8.561755016148066 1105 5 8.261709877347158 1105 6 7.423588524999463 1105 7 7.4404924533188925 1105 8 6.653566115062649 1105 9 6.654599758846114 1105 10-11 6.080535497215223 1105 12-13 5.941036203637612 1105 14-15 6.130425961003549 1105 16-17 6.797684135958196 1105 18-19 7.260893803129779 1105 20-21 7.767067812737974 1105 22-23 7.544954430549513 1105 24-25 7.72546896294179 1105 26-27 7.686638627730609 1105 28-29 7.554307264141396 1105 30-31 7.39692196309673 1105 32-33 7.140827443819948 1105 34-35 8.924892658919472 1105 36-37 -3.260993559029087 1105 38-39 -2.1693655850439058 1105 40-41 5.077058052444794 1105 42-43 6.865878344216636 1105 44-45 -2.962901752002457 1105 46-47 -3.9481730878149026 1105 48-49 4.072146823575363 1105 50-51 -8.693468852968437 1105 52-53 0.3891656516540216 1105 54-55 4.709743349417778 1105 56-57 5.837195796644167 1105 58-59 6.027125066689326 1105 60-61 5.985257809671726 1105 62-63 6.060974341287881 1105 64-65 5.847927537738762 1105 66-67 5.790265939328602 1105 68-69 5.6668546687021895 1105 70-71 5.762062291709251 1105 72-73 5.659537566837052 1105 74-75 5.522183398118621 1105 76-77 5.338043091640506 1105 78-79 5.165592295869795 1105 80-81 5.151773664947996 1105 82-83 5.030793963293251 1105 84-85 4.75650972088723 1105 86-87 4.649672072077861 1105 88-89 4.610313752053198 1105 90-91 4.342642574031121 1105 92-93 4.00226782988687 1105 94-95 3.792262428754972 1105 96-97 3.5720538215590256 1105 98-99 3.2283621507925124 1105 100-101 2.89623280430666 1106 1 5.862213679667292 1106 2 6.838759664771672 1106 3 6.540109312107575 1106 4 7.948072783641731 1106 5 7.7452066552905805 1106 6 6.857798036215282 1106 7 6.781319989138655 1106 8 6.20561417165214 1106 9 6.238902377499066 1106 10-11 5.822545529305717 1106 12-13 5.677124124880521 1106 14-15 5.945006482943331 1106 16-17 6.237229196239944 1106 18-19 6.6095199730016425 1106 20-21 7.047991578742053 1106 22-23 6.735924879223639 1106 24-25 6.8684271457061215 1106 26-27 6.944698011731045 1106 28-29 6.900681528015433 1106 30-31 6.422646667644319 1106 32-33 6.405552642625732 1106 34-35 8.463902269098579 1106 36-37 -0.08913067788673956 1106 38-39 0.9870811741261925 1106 40-41 5.851539435813979 1106 42-43 7.161691903593546 1106 44-45 -3.09043527185211 1106 46-47 -4.1038558233994955 1106 48-49 3.7026354815945766 1106 50-51 -8.67771382863345 1106 52-53 0.06750156234233629 1106 54-55 4.196134446205409 1106 56-57 5.206562779950925 1106 58-59 5.576795866390601 1106 60-61 5.512041395699729 1106 62-63 5.411114518807739 1106 64-65 5.464593992277447 1106 66-67 5.433424638859034 1106 68-69 5.043175011876141 1106 70-71 5.039622294062758 1106 72-73 4.866976431831766 1106 74-75 4.760353132211726 1106 76-77 4.632940425604863 1106 78-79 4.526740520073888 1106 80-81 4.2568812378387975 1106 82-83 4.070585348820563 1106 84-85 3.960287480206148 1106 86-87 3.7192606565556545 1106 88-89 3.648813054877838 1106 90-91 3.341461495668476 1106 92-93 3.3641337444593606 1106 94-95 3.1811152093676345 1106 96-97 3.0779212815396857 1106 98-99 2.756149492937195 1106 100-101 2.518447242920562 1107 1 5.874828317092685 1107 2 7.002300376506636 1107 3 6.5837071181825735 1107 4 7.573505312750456 1107 5 7.972117837559484 1107 6 6.482610782506789 1107 7 6.992667920547145 1107 8 6.417592669037141 1107 9 6.56369528277747 1107 10-11 6.009542764503674 1107 12-13 5.6400532821782825 1107 14-15 5.917391309677871 1107 16-17 6.817288845359258 1107 18-19 6.9988408794904196 1107 20-21 7.435238800751382 1107 22-23 7.205633823505895 1107 24-25 7.187238549577241 1107 26-27 7.26454252520983 1107 28-29 7.196567520492437 1107 30-31 7.139051651919157 1107 32-33 6.653139371575929 1107 34-35 8.776686799346006 1107 36-37 0.13923747509742412 1107 38-39 1.1466657935586397 1107 40-41 6.373534592350616 1107 42-43 7.332244737922082 1107 44-45 -2.9601494915876803 1107 46-47 -3.842821001757926 1107 48-49 4.067869713380741 1107 50-51 -8.612121031916573 1107 52-53 0.39934200874522574 1107 54-55 4.657874469875278 1107 56-57 5.911740338769743 1107 58-59 6.249421555724798 1107 60-61 6.29261737566495 1107 62-63 6.256982777135075 1107 64-65 6.128715501561416 1107 66-67 6.0188978486804 1107 68-69 5.636076076555522 1107 70-71 5.668416265712667 1107 72-73 5.587906444482904 1107 74-75 5.617358569246541 1107 76-77 5.5293374071165555 1107 78-79 5.521811206171476 1107 80-81 5.350648439051557 1107 82-83 5.17492303224625 1107 84-85 4.990419111061115 1107 86-87 4.8791085509696135 1107 88-89 4.7325543234219705 1107 90-91 4.52278571076231 1107 92-93 4.324760365458518 1107 94-95 3.997601929989914 1107 96-97 3.6980605236461646 1107 98-99 3.6005648449708483 1107 100-101 3.315668522307572 1108 1 5.964582618305894 1108 2 6.302793963071725 1108 3 6.574490754983685 1108 4 7.885150237945879 1108 5 7.962064871941273 1108 6 7.4248987306019885 1108 7 7.627100801149183 1108 8 6.60270059207858 1108 9 4.937167918389704 1108 10-11 5.953561577866449 1108 12-13 5.562994023874182 1108 14-15 6.139306249633478 1108 16-17 6.122713312523722 1108 18-19 6.7862637718217265 1108 20-21 7.625438344608089 1108 22-23 7.798170171178008 1108 24-25 7.311007574195422 1108 26-27 7.669892017199846 1108 28-29 7.570655366848271 1108 30-31 7.33416036370652 1108 32-33 7.296171742949415 1108 34-35 9.293889879709024 1108 36-37 -3.017370299278701 1108 38-39 -1.9037145215586513 1108 40-41 5.260118467282101 1108 42-43 7.4547548708700795 1108 44-45 -2.658935897418729 1108 46-47 -3.6228312332991663 1108 48-49 4.574851554942956 1108 50-51 -8.64521479475308 1108 52-53 0.6489070906972536 1108 54-55 5.113492722608083 1108 56-57 6.309470622669533 1108 58-59 6.715269964961632 1108 60-61 6.6490197548417065 1108 62-63 6.709475734919 1108 64-65 6.672675181135599 1108 66-67 6.592651685508365 1108 68-69 6.521426040463748 1108 70-71 6.3766292931366575 1108 72-73 6.3249677987242094 1108 74-75 6.169369162370181 1108 76-77 5.843126624516648 1108 78-79 6.024485187791926 1108 80-81 5.812826908769253 1108 82-83 5.655256450214681 1108 84-85 5.557266768330628 1108 86-87 5.334643110226809 1108 88-89 5.039627734841149 1108 90-91 4.897542827975716 1108 92-93 4.828354642973897 1108 94-95 4.470398780943963 1108 96-97 4.30848157438867 1108 98-99 4.098829326988549 1108 100-101 3.5438434683693174 1109 1 4.686719346638039 1109 2 6.093521738640675 1109 3 5.871196375469751 1109 4 7.418108553031171 1109 5 7.073190783306142 1109 6 4.566754790061335 1109 7 4.257840188189945 1109 8 2.8608355739807863 1109 9 2.3165363758646507 1109 10-11 2.352219615312439 1109 12-13 2.9901600129807484 1109 14-15 3.4136239355106532 1109 16-17 2.4275266129287303 1109 18-19 3.3103912127703943 1109 20-21 3.478851697980069 1109 22-23 5.201956384427689 1109 24-25 5.125724458795823 1109 26-27 3.974939038278773 1109 28-29 3.7740614490349813 1109 30-31 3.8131085163993923 1109 32-33 3.4856756216250027 1109 34-35 5.5501820057722036 1109 36-37 -4.561804949767346 1109 38-39 -3.4866203574108283 1109 40-41 2.243264790439362 1109 42-43 2.8904551403916727 1109 44-45 -4.536490791223544 1109 46-47 -5.714508714242157 1109 48-49 1.1418924998449747 1109 50-51 -9.06541502200425 1109 52-53 -1.4595953075044683 1109 54-55 1.692000977001122 1109 56-57 3.0547175828431676 1109 58-59 3.68895505771998 1109 60-61 3.947177369919846 1109 62-63 4.052875847670027 1109 64-65 3.8053585871135134 1109 66-67 3.94326319387174 1109 68-69 4.000177613271017 1109 70-71 3.96057568773878 1109 72-73 3.725677269070218 1109 74-75 3.7581599210917354 1109 76-77 3.8356850961706837 1109 78-79 3.9192003871816166 1109 80-81 3.693314551342226 1109 82-83 3.5726933467539563 1109 84-85 3.52003544058865 1109 86-87 3.4793398653608705 1109 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-12.93881594407809 2313 74-75 -12.415321878306997 2313 76-77 -11.89755867198949 2313 78-79 -11.420531980187565 2313 80-81 -10.869488554912861 2313 82-83 -10.391675076658053 2313 84-85 -9.888273438046156 2313 86-87 -9.362614514614762 2313 88-89 -8.852137896572021 2313 90-91 -8.285913623211014 2313 92-93 -7.712310097536554 2313 94-95 -7.207381614909005 2313 96-97 -6.5415307061828445 2313 98-99 -5.809014912041352 2313 100-101 -4.960251174277898 2314 1 -19.91689711779213 2314 2 -18.927177761716212 2314 3 -19.304868340209527 2314 4 -21.188600940187957 2314 5 -21.16140355407192 2314 6 -21.82439438909641 2314 7 -21.86064660695638 2314 8 -22.542209869749883 2314 9 -23.538091271054217 2314 10-11 -23.682041626657583 2314 12-13 -23.5559974935622 2314 14-15 -23.71330920748245 2314 16-17 -22.904855019238767 2314 18-19 -22.333434625916258 2314 20-21 -21.835447612036585 2314 22-23 -21.71679212853924 2314 24-25 -21.390335498377624 2314 26-27 -21.015781875354346 2314 28-29 -20.866314472925524 2314 30-31 -20.715085630638438 2314 32-33 -20.349799039331455 2314 34-35 -17.674300506718875 2314 36-37 -16.096423079674555 2314 38-39 -15.221928470663268 2314 40-41 -16.29266192857087 2314 42-43 -16.16105568520909 2314 44-45 -14.345198856890924 2314 46-47 -14.956478735655434 2314 48-49 -14.39522384562255 2314 50-51 -12.667128084102751 2314 52-53 -15.216078771696711 2314 54-55 -16.556511989927262 2314 56-57 -16.68499214830125 2314 58-59 -16.306643324388038 2314 60-61 -15.96455234694906 2314 62-63 -15.481028506362808 2314 64-65 -14.977772509722083 2314 66-67 -14.532230501108035 2314 68-69 -14.056448606096101 2314 70-71 -13.49719257302734 2314 72-73 -13.0209322955265 2314 74-75 -12.50593715242718 2314 76-77 -11.942806457481469 2314 78-79 -11.463026593546436 2314 80-81 -10.947894109138382 2314 82-83 -10.478579553555347 2314 84-85 -9.979726634119892 2314 86-87 -9.449758397784501 2314 88-89 -8.939401482877983 2314 90-91 -8.374134834606753 2314 92-93 -7.800651012068515 2314 94-95 -7.207381614909005 2314 96-97 -6.564633411473723 2314 98-99 -5.862881323341328 2314 100-101 -5.021778586296094 2315 1 -19.91689711779213 2315 2 -18.927177761716212 2315 3 -19.304868340209527 2315 4 -17.45218920377622 2315 5 -21.16140355407192 2315 6 -21.82439438909641 2315 7 -21.86064660695638 2315 8 -22.542209869749883 2315 9 -20.074166307129254 2315 10-11 -18.81984826446422 2315 12-13 -16.27846021602492 2315 14-15 -16.218359712532955 2315 16-17 -19.980973345357093 2315 18-19 -22.333434625916258 2315 20-21 -21.835447612036585 2315 22-23 -21.71679212853924 2315 24-25 -21.390335498377624 2315 26-27 -21.015781875354346 2315 28-29 -20.866314472925524 2315 30-31 -20.715085630638438 2315 32-33 -20.349799039331455 2315 34-35 -17.674300506718875 2315 36-37 -16.096423079674555 2315 38-39 -15.221928470663268 2315 40-41 -16.29266192857087 2315 42-43 -16.16105568520909 2315 44-45 -14.345198856890924 2315 46-47 -14.956478735655434 2315 48-49 -14.39522384562255 2315 50-51 -12.667128084102751 2315 52-53 -15.216078771696711 2315 54-55 -16.556511989927262 2315 56-57 -16.68499214830125 2315 58-59 -16.306643324388038 2315 60-61 -15.96455234694906 2315 62-63 -15.481028506362808 2315 64-65 -14.977772509722083 2315 66-67 -14.532230501108035 2315 68-69 -14.056448606096101 2315 70-71 -13.49719257302734 2315 72-73 -13.0209322955265 2315 74-75 -12.50593715242718 2315 76-77 -11.942806457481469 2315 78-79 -11.463026593546436 2315 80-81 -10.947894109138382 2315 82-83 -10.478579553555347 2315 84-85 -9.979726634119892 2315 86-87 -9.449758397784501 2315 88-89 -8.939401482877983 2315 90-91 -8.374134834606753 2315 92-93 -7.800651012068515 2315 94-95 -7.207381614909005 2315 96-97 -6.564633411473723 2315 98-99 -5.862881323341328 2315 100-101 -5.021778586296094 2316 1 -19.91689711779213 2316 2 -18.927177761716212 2316 3 -19.304868340209527 2316 4 -21.188600940187957 2316 5 -21.16140355407192 2316 6 -21.82439438909641 2316 7 -21.86064660695638 2316 8 -22.542209869749883 2316 9 -23.538091271054217 2316 10-11 -23.682041626657583 2316 12-13 -23.5559974935622 2316 14-15 -23.71330920748245 2316 16-17 -22.904855019238767 2316 18-19 -22.333434625916258 2316 20-21 -21.835447612036585 2316 22-23 -21.71679212853924 2316 24-25 -21.390335498377624 2316 26-27 -21.015781875354346 2316 28-29 -20.866314472925524 2316 30-31 -20.715085630638438 2316 32-33 -20.349799039331455 2316 34-35 -17.674300506718875 2316 36-37 -16.096423079674555 2316 38-39 -15.221928470663268 2316 40-41 -16.29266192857087 2316 42-43 -16.16105568520909 2316 44-45 -14.345198856890924 2316 46-47 -14.956478735655434 2316 48-49 -14.39522384562255 2316 50-51 -12.667128084102751 2316 52-53 -15.216078771696711 2316 54-55 -16.556511989927262 2316 56-57 -16.68499214830125 2316 58-59 -16.306643324388038 2316 60-61 -15.96455234694906 2316 62-63 -15.481028506362808 2316 64-65 -14.977772509722083 2316 66-67 -14.532230501108035 2316 68-69 -14.056448606096101 2316 70-71 -13.49719257302734 2316 72-73 -13.0209322955265 2316 74-75 -12.50593715242718 2316 76-77 -11.942806457481469 2316 78-79 -11.463026593546436 2316 80-81 -10.947894109138382 2316 82-83 -10.478579553555347 2316 84-85 -9.979726634119892 2316 86-87 -9.449758397784501 2316 88-89 -8.939401482877983 2316 90-91 -8.374134834606753 2316 92-93 -7.800651012068515 2316 94-95 -7.207381614909005 2316 96-97 -6.564633411473723 2316 98-99 -5.862881323341328 2316 100-101 -5.021778586296094 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 729693.0 3 47377.0 4 88040.0 5 63837.0 6 58479.0 7 72153.0 8 89761.0 9 102952.0 10 104447.0 11 95844.0 12 83301.0 13 75202.0 14 72426.0 15 75443.0 16 80175.0 17 83903.0 18 85815.0 19 88817.0 20 92538.0 21 96473.0 22 101097.0 23 105039.0 24 109633.0 25 114885.0 26 119410.0 27 124702.0 28 129852.0 29 134043.0 30 134957.0 31 130585.0 32 124601.0 33 116937.0 34 101354.0 35 77933.0 36 45971.0 37 14943.0 38 1295.0 39 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.90855736112611 19.82929460183007 13.688936241686744 23.57321179535708 2 15.628887847770734 20.71613415394508 40.32896415966768 23.32601383861651 3 19.06672881101535 26.35943294699723 29.386326582326173 25.18751165966125 4 12.771638809353725 16.608077744839083 37.97340724750802 32.64687619829917 5 13.439738030280862 37.91474978085026 34.52848169695055 14.117030491918328 6 30.781023312990964 36.749278653237944 18.25281920982292 14.216878823948173 7 27.30155902637121 32.50114440649319 22.572785584532063 17.624510982603542 8 26.049045650966452 34.906359303161096 20.759942322126747 18.284652723745708 9 27.134322765538617 15.831814862657767 20.71411111209142 36.3197512597122 10-11 25.337468811326847 26.152798357902313 28.209175017625526 20.300557813145314 12-13 25.703303402975138 23.941207544678637 28.494442054729983 21.861046997616242 14-15 22.909077120211894 24.99930684936124 26.473950855347887 25.61766517507898 16-17 22.478407224597134 27.91124149236048 26.929581757568116 22.680769525474265 18-19 22.34895592973374 26.942140384921597 29.08930405577488 21.619599629569784 20-21 23.234726031350878 26.158637031686855 29.017025263341733 21.58961167362054 22-23 22.97918343423854 26.04112806372523 28.728779314988795 22.250909187047437 24-25 22.66898915296712 26.339777463946152 28.743476378052357 22.24775700503437 26-27 22.484475498749276 26.507831012213966 28.742228349788395 22.265465139248363 28-29 22.487750892349265 26.704428302198345 28.71820386714081 22.089616938311575 30-31 22.821734843714772 26.525426649268542 28.448166596770445 22.20467191024624 32-33 22.672978391368257 26.682390018326213 28.352569257073785 22.292062333231744 34-35 22.948161053665874 26.579695561696514 28.469638985438124 22.002504399199488 36-37 23.417000245584642 26.513797805283623 28.009730774732734 22.059471174399 38-39 23.286820560831924 26.557670458814375 28.02286199243574 22.13264698791796 40-41 23.137460755355992 26.47019649935548 28.224420756965944 22.167921988322583 42-43 23.28914952266349 26.473076397021543 27.808000672973026 22.429773407341948 44-45 23.51181671653572 26.735832137909792 27.525550433849272 22.226800711705213 46-47 22.976630280758258 26.584164377674046 27.818641113600616 22.62056422796708 48-49 23.476997198502556 26.693808426282644 27.240985659249144 22.588208715965653 50-51 23.600664918041367 26.86613250456345 27.067468564079906 22.465734013315274 52-53 22.735695341801446 26.981809896392374 27.522526677699215 22.759968084106966 54-55 22.541507872284715 26.906622407769827 27.695121137944046 22.856748582001412 56-57 22.682195260744063 27.139595991264986 27.333050243554823 22.845158504436125 58-59 22.7001394270754 26.998910984649083 27.562994506128852 22.73795508214667 60-61 22.71397072983556 27.104062639969477 27.55751866439999 22.624447965794975 62-63 22.61275738588724 26.974524014992745 27.554537583151834 22.858181015968178 64-65 22.463389609995257 26.87189195628359 27.544172469126867 23.12054596459429 66-67 22.63827177829623 27.006477209957307 27.30774312224966 23.047507889496803 68-69 22.514504326380376 26.92098300276063 27.44649682562126 23.118015845237736 70-71 22.45730931183616 26.746765262718785 27.414884248282355 23.3810411771627 72-73 22.744503808818088 26.8897488527392 27.212655305290156 23.153092033152557 74-75 22.628466479888782 27.107777073935985 27.08039810047743 23.1833583456978 76-77 22.67062116768862 26.869643272432313 27.18887729229652 23.270858267582543 78-79 22.559872007111984 27.113065133335425 27.010702165792388 23.316360693760206 80-81 22.697113682587393 27.10556828192327 26.947454674241538 23.249863361247797 82-83 22.89880784158631 26.938752416849592 27.070031849337035 23.092407892227065 84-85 22.838632768966953 27.03871101939153 26.769282805623227 23.35337340601829 86-87 22.578705674419698 27.160733236503326 26.88072394396115 23.379837145115825 88-89 22.719871179025937 26.959777467233543 26.74619566308225 23.574155690658273 90-91 22.807049598163605 26.913709332941337 26.784456180302197 23.49478488859286 92-93 22.882359103289335 26.830298242931804 26.750131652708646 23.53721100107021 94-95 22.67850159953296 26.706481485287288 26.80738153527668 23.807635379903076 96-97 22.651637909015353 26.692099789724704 26.686486430833195 23.96977587042675 98-99 22.505596160557335 26.761831545466613 26.536887077708254 24.195685216267798 100-101 23.039443910925275 26.801804182818483 26.33958065252284 23.8191712537334 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 46949.0 1 51077.5 2 38348.0 3 22551.5 4 28758.0 5 36391.0 6 38727.0 7 37322.0 8 34565.0 9 31583.0 10 28446.0 11 24980.0 12 21697.0 13 18773.5 14 16206.0 15 14151.5 16 12558.0 17 11608.5 18 11292.0 19 11417.0 20 12009.5 21 13067.5 22 14577.0 23 16724.5 24 19665.5 25 23503.0 26 28006.0 27 33579.0 28 40229.0 29 48120.5 30 56333.5 31 64274.0 32 72504.0 33 79034.5 34 84056.5 35 88854.5 36 93677.5 37 97809.0 38 99873.0 39 100608.0 40 101259.0 41 102467.5 42 104331.0 43 107427.5 44 110670.0 45 113730.5 46 116757.0 47 119156.5 48 121076.0 49 121498.5 50 120400.5 51 118989.0 52 117059.5 53 114220.0 54 109417.0 55 101992.0 56 93366.5 57 83621.5 58 73465.5 59 64031.0 60 54877.0 61 45714.5 62 37274.0 63 29818.0 64 23192.5 65 17792.0 66 13457.0 67 10041.5 68 7394.5 69 5406.0 70 3970.0 71 2798.5 72 1996.5 73 1474.5 74 1109.0 75 809.0 76 542.5 77 332.5 78 207.0 79 132.0 80 86.0 81 43.0 82 27.5 83 23.0 84 12.5 85 4.0 86 3.0 87 2.5 88 2.0 89 1.5 90 1.0 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.453627102334321 2 21.419803538877016 3 17.178370151759626 4 22.43182795006236 5 22.262775559422998 6 16.154191482658415 7 14.958418849316393 8 12.062031507460148 9 3.0261837441959014 10-11 6.503018684843867 12-13 3.5612101513972614 14-15 7.424968217747935 16-17 11.492681274591613 18-19 14.58220304606338 20-21 14.990276593667303 22-23 12.909042365263886 24-25 15.45103117428753 26-27 12.992650071113731 28-29 12.982383120327656 30-31 13.83501815337818 32-33 12.312540073821388 34-35 19.174235187658724 36-37 23.91893789400682 38-39 22.966715954740867 40-41 18.097325157351086 42-43 18.035798944919822 44-45 23.35915001726257 46-47 12.761807245044936 48-49 21.47160382856608 50-51 21.152875400486572 52-53 11.20472853477527 54-55 12.835072507823517 56-57 12.353607876965693 58-59 13.359555159193098 60-61 12.777283163509242 62-63 12.739033739012099 64-65 13.813377031623213 66-67 12.099463098867716 68-69 13.149044921935946 70-71 11.77751366662003 72-73 11.128644893349533 74-75 11.214655770277982 76-77 11.272910650350939 78-79 11.684846886547176 80-81 11.646773610715476 82-83 10.1579650903542 84-85 10.0529175755099 86-87 10.203109476898858 88-89 11.611833264719234 90-91 9.099965877487094 92-93 11.119912952362355 94-95 11.227401384427854 96-97 10.790401709547963 98-99 10.623035313277885 100-101 10.737884243149578 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3973916.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.94372192986275 #Duplication Level Percentage of deduplicated Percentage of total 1 98.52339313046309 94.52701034095699 2 1.011161126662688 1.9402912392562335 3 0.22794000125277789 0.6560823629066725 4 0.08001392569219197 0.3070733534851347 5 0.03815837227478135 0.1830528129413903 6 0.024700084137385166 0.14218908024728977 7 0.018103702890801522 0.12158556452391395 8 0.010411732066580977 0.07991522609634243 9 0.009877111703050922 0.08528821728369396 >10 0.050216998329472996 0.899452458553395 >50 0.0035244879599230913 0.23705844261740847 >100 0.0023156871958953393 0.3984459397716521 >500 7.870261105138104E-5 0.04457619464258849 >1k 7.870257024233563E-5 0.19506795245781697 >5k 2.6234190080778543E-5 0.18291081425947817 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 7267 0.18286747882944682 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.0328190127823536E-5 2 0.0 2.5164095063911768E-5 0.0 0.0 5.0328190127823536E-5 3 0.0 2.5164095063911768E-5 0.0 0.0 5.0328190127823536E-5 4 0.0 2.5164095063911768E-5 0.0 0.0 5.0328190127823536E-5 5 0.0 2.5164095063911768E-5 0.0 0.0 5.0328190127823536E-5 6 0.0 2.5164095063911768E-5 0.0 0.0 5.0328190127823536E-5 7 0.0 2.5164095063911768E-5 0.0 0.0 5.0328190127823536E-5 8 0.0 2.5164095063911768E-5 0.0 0.0 5.0328190127823536E-5 9 0.0 2.5164095063911768E-5 0.0 0.0 5.0328190127823536E-5 10-11 0.0 2.5164095063911768E-5 0.0 1.2582047531955884E-5 7.54922851917353E-5 12-13 2.5164095063911768E-5 2.5164095063911768E-5 0.0 2.5164095063911768E-5 2.7680504570302944E-4 14-15 2.5164095063911768E-5 2.5164095063911768E-5 0.0 2.5164095063911768E-5 3.7746142595867653E-4 16-17 2.5164095063911768E-5 2.5164095063911768E-5 0.0 6.291023765977942E-5 5.284459963421471E-4 18-19 2.5164095063911768E-5 2.5164095063911768E-5 0.0 1.0065638025564707E-4 5.536100914060589E-4 20-21 2.5164095063911768E-5 3.774614259586765E-5 0.0 1.0065638025564707E-4 6.039382815338824E-4 22-23 5.0328190127823536E-5 5.0328190127823536E-5 0.0 1.8873071297933827E-4 6.416844241297501E-4 24-25 5.0328190127823536E-5 5.0328190127823536E-5 0.0 3.1455118829889714E-4 6.54266471661706E-4 26-27 5.0328190127823536E-5 6.291023765977942E-5 0.0 5.661921389380148E-4 7.171767093214854E-4 28-29 5.0328190127823536E-5 1.0065638025564707E-4 0.0 0.0012204586105997207 7.297587568534413E-4 30-31 7.54922851917353E-5 1.0065638025564707E-4 2.5164095063911768E-5 0.003736868116990898 7.297587568534413E-4 32-33 7.54922851917353E-5 1.0065638025564707E-4 2.5164095063911768E-5 0.010204040548416221 7.423408043853972E-4 34-35 7.54922851917353E-5 1.0065638025564707E-4 2.5164095063911768E-5 0.020496155429556136 8.052510420451766E-4 36-37 7.54922851917353E-5 1.0065638025564707E-4 2.5164095063911768E-5 0.03573301499075471 8.304151371090883E-4 38-39 7.54922851917353E-5 1.1323842778760296E-4 2.5164095063911768E-5 0.05859459535631855 8.304151371090883E-4 40-41 7.54922851917353E-5 1.2582047531955884E-4 2.5164095063911768E-5 0.0848784926505744 8.555792321730001E-4 42-43 7.54922851917353E-5 1.2582047531955884E-4 2.5164095063911768E-5 0.11068427213861591 8.681612797049559E-4 44-45 7.54922851917353E-5 1.2582047531955884E-4 2.5164095063911768E-5 0.14245394215680451 8.807433272369119E-4 46-47 7.54922851917353E-5 1.2582047531955884E-4 2.5164095063911768E-5 0.18028815908539586 8.933253747688678E-4 48-49 1.0065638025564707E-4 1.2582047531955884E-4 2.5164095063911768E-5 0.2220983030340853 9.184894698327797E-4 50-51 1.0065638025564707E-4 1.2582047531955884E-4 2.5164095063911768E-5 0.2710550499809256 9.813997074925589E-4 52-53 1.0065638025564707E-4 1.2582047531955884E-4 2.5164095063911768E-5 0.337400186616929 0.0010065638025564707 54-55 1.1323842778760296E-4 1.2582047531955884E-4 2.5164095063911768E-5 0.40689083513592134 0.0010443099451523385 56-57 1.2582047531955884E-4 1.2582047531955884E-4 2.5164095063911768E-5 0.4886489799985707 0.0010694740402162502 58-59 1.2582047531955884E-4 1.2582047531955884E-4 2.5164095063911768E-5 0.5777550406198823 0.0010820560877482061 60-61 1.2582047531955884E-4 1.2582047531955884E-4 2.5164095063911768E-5 0.675353983325264 0.0010820560877482061 62-63 1.2582047531955884E-4 1.2582047531955884E-4 2.5164095063911768E-5 0.7755699919172927 0.0010820560877482061 64-65 1.2582047531955884E-4 1.2582047531955884E-4 2.5164095063911768E-5 0.8777739640193704 0.0010820560877482061 66-67 1.2582047531955884E-4 1.2582047531955884E-4 2.5164095063911768E-5 0.9906978406186744 0.0010946381352801618 68-69 1.2582047531955884E-4 1.509845703834706E-4 2.5164095063911768E-5 1.114316457620141 0.0011072201828121178 70-71 1.2582047531955884E-4 1.7614866544738239E-4 2.5164095063911768E-5 1.2532725905630617 0.0011323842778760296 72-73 1.2582047531955884E-4 1.7614866544738239E-4 2.5164095063911768E-5 1.3973621988990206 0.0011575483729399415 74-75 1.2582047531955884E-4 1.7614866544738239E-4 2.5164095063911768E-5 1.543225875936985 0.0011952945155358089 76-77 1.2582047531955884E-4 1.8873071297933827E-4 2.5164095063911768E-5 1.701533197984054 0.0012078765630677648 78-79 1.2582047531955884E-4 2.2647685557520593E-4 2.5164095063911768E-5 1.879544006466166 0.0012582047531955883 80-81 1.2582047531955884E-4 2.2647685557520593E-4 2.5164095063911768E-5 2.0720241696100272 0.0012707868007275443 82-83 1.509845703834706E-4 2.2647685557520593E-4 2.5164095063911768E-5 2.27300979688549 0.0013588611334512356 84-85 1.7614866544738239E-4 2.2647685557520593E-4 2.5164095063911768E-5 2.475379449389469 0.001409189323579059 86-87 1.7614866544738239E-4 2.516409506391177E-4 2.5164095063911768E-5 2.684983779224322 0.0014217713711110148 88-89 1.7614866544738239E-4 2.516409506391177E-4 2.5164095063911768E-5 2.9089945534832644 0.0014469354661749267 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3730 0.0 55.806835 1 GTATCAA 7675 0.0 50.303318 1 ATCAACG 9585 0.0 39.8884 3 TCAACGC 9915 0.0 38.54844 4 TATCAAC 9940 0.0 38.421566 2 CAACGCA 10035 0.0 37.865765 5 AACGCAG 10905 0.0 34.711464 6 GTACATG 12030 0.0 30.919819 1 TACATGG 12290 0.0 29.640032 2 ACGCAGA 12570 0.0 29.474207 7 CGCAGAG 12915 0.0 28.876038 8 ACATGGG 13145 0.0 27.706053 3 GCAGAGT 13490 0.0 25.537493 9 CATGGGG 9270 0.0 23.738836 4 CATGGGA 5045 0.0 21.360445 4 GTGGTAT 1720 0.0 21.098436 1 GAGTACA 8555 0.0 20.914839 1 AGAGTAC 12670 0.0 20.269705 10-11 GAGTACT 7750 0.0 20.182789 12-13 CAGAGTA 13090 0.0 19.872818 10-11 >>END_MODULE