FastQCFastQC Report
Sat 18 Jun 2016
SRR1930948_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930948_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3973916
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111040.2794221115896763No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84880.2135928389024831No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62710.1578040401457907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55800.055.5677721
GTATCAA110350.044.5791131
CAACGCA144850.033.4179425
ATCAACG144950.033.4074973
TCAACGC145650.033.2786984
TATCAAC147300.033.197242
AACGCAG153600.031.6996296
GTGGTAT22450.030.0341971
ACGCAGA181700.026.5360857
GTACATG177000.026.1562421
CGCAGAG185600.025.9017758
TACATGG180000.025.5047722
ACATGGG180950.024.6884043
TGGTATC26300.023.8278942
GCAGAGT204650.023.0269039
CATGGGG116100.022.203364
AGAGTAC198250.020.28731310-11
CATGGGA69600.019.7806174
GAGTACA131150.019.6234321
AGTACTT131450.019.17043512-13
Produced by FastQC (version 0.11.5)