##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930948_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3973916 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.24610283659745 31.0 31.0 33.0 30.0 34.0 2 31.356876189632594 31.0 31.0 34.0 30.0 34.0 3 31.436791567813714 31.0 31.0 34.0 28.0 34.0 4 35.30730418056144 37.0 35.0 37.0 33.0 37.0 5 35.097186754828236 37.0 35.0 37.0 32.0 37.0 6 35.138755575105264 37.0 35.0 37.0 32.0 37.0 7 35.01965567465442 36.0 35.0 37.0 32.0 37.0 8 34.998907878274224 36.0 35.0 37.0 32.0 37.0 9 36.49223838651849 38.0 35.0 39.0 32.0 39.0 10-11 36.42120794702254 38.0 35.0 39.0 32.0 39.0 12-13 36.31056796369123 38.0 35.0 39.0 32.0 39.0 14-15 37.31020736220897 39.0 36.0 41.0 32.0 41.0 16-17 37.282748553316175 39.0 36.0 40.5 32.0 41.0 18-19 37.28672045408106 39.0 36.0 40.0 32.0 41.0 20-21 37.25588210722119 39.0 36.0 40.0 31.0 41.0 22-23 37.120968334509335 39.0 36.0 40.0 31.0 41.0 24-25 36.98923781982307 39.0 36.0 40.0 31.0 41.0 26-27 36.70785945148312 38.5 35.0 40.0 30.0 41.0 28-29 36.51881406149501 38.0 35.0 40.0 30.0 41.0 30-31 36.26859827937984 38.0 35.0 40.0 30.0 41.0 32-33 36.01924097036777 38.0 34.5 40.0 29.5 41.0 34-35 36.19268474723673 38.0 35.0 40.0 30.0 41.0 36-37 36.23522754381321 38.0 35.0 40.0 30.0 41.0 38-39 36.18852260591316 38.0 35.0 40.0 30.0 41.0 40-41 36.07761298930325 38.0 35.0 40.0 29.5 41.0 42-43 35.91913379648689 38.0 35.0 40.0 29.0 41.0 44-45 35.697851942517154 38.0 34.0 40.0 28.5 41.0 46-47 35.42766618620021 38.0 34.0 40.0 27.0 41.0 48-49 35.192244627214066 38.0 34.0 40.0 27.0 41.0 50-51 34.898928789637225 38.0 33.5 40.0 26.0 41.0 52-53 34.66998723677098 38.0 33.0 40.0 26.0 41.0 54-55 34.48403866614191 37.5 33.0 40.0 26.0 41.0 56-57 34.20747091785533 37.0 33.0 40.0 25.0 41.0 58-59 33.94646665908388 37.0 32.5 40.0 24.5 41.0 60-61 33.619159916817566 36.0 32.0 39.5 23.5 41.0 62-63 33.29602676553807 36.0 32.0 39.0 23.0 41.0 64-65 32.97347037531745 35.5 31.0 39.0 22.5 40.0 66-67 32.6264110766307 35.0 31.0 39.0 22.0 40.0 68-69 32.24598745922158 35.0 31.0 38.0 21.5 40.0 70-71 31.847551885847615 35.0 30.5 37.5 20.5 40.0 72-73 31.433868129069662 34.5 30.0 37.0 20.0 39.0 74-75 30.912385163652175 34.0 30.0 36.0 18.0 39.0 76-77 29.56517022503747 32.5 28.5 35.0 16.0 37.5 78-79 30.326331507762117 34.0 29.0 35.0 18.0 38.0 80-81 30.33600118875185 34.0 30.0 35.0 18.0 37.0 82-83 30.068246913120458 34.0 29.0 35.0 17.0 37.0 84-85 29.75865921675244 34.0 29.0 35.0 13.0 36.0 86-87 29.430793076652854 34.0 29.0 35.0 8.5 36.0 88-89 29.08661091477525 34.0 29.0 35.0 7.0 36.0 90-91 28.747876150376605 33.5 29.0 35.0 2.0 35.0 92-93 28.38316813943727 33.0 29.0 35.0 2.0 35.0 94-95 27.963219907013638 33.0 27.0 35.0 2.0 35.0 96-97 27.495728772324327 33.0 27.0 35.0 2.0 35.0 98-99 27.001083691753927 33.0 26.0 35.0 2.0 35.0 100-101 25.78081582499479 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4967785328678289 1101 2 0.5992530568314827 1101 3 0.56293859360742 1101 4 0.3753011963859265 1101 5 0.4913139832593387 1101 6 0.4726836726340409 1101 7 0.49054872615585055 1101 8 0.4616159807005076 1101 9 0.636512602363041 1101 10-11 0.6094615839777546 1101 12-13 0.6187194480817837 1101 14-15 0.7522821065742704 1101 16-17 0.7692755510427318 1101 18-19 0.7690182246000035 1101 20-21 0.7086600411039967 1101 22-23 0.7573901518573791 1101 24-25 0.6934931693491109 1101 26-27 0.6649161144267666 1101 28-29 0.6787168434896742 1101 30-31 0.7353707286921747 1101 32-33 0.7429056474779188 1101 34-35 0.7136146837691797 1101 36-37 0.7114950610458948 1101 38-39 0.8009686380794037 1101 40-41 0.8477529906991208 1101 42-43 0.8084450963551149 1101 44-45 0.8771885924385785 1101 46-47 0.9309576297451443 1101 48-49 0.869893692625773 1101 50-51 0.9397001529064468 1101 52-53 0.9321259788524685 1101 54-55 0.969646700769637 1101 56-57 1.1239698573167445 1101 58-59 1.0037097915320317 1101 60-61 1.0128997736185 1101 62-63 1.0591084457467375 1101 64-65 1.1043660645140818 1101 66-67 1.2383708757666767 1101 68-69 1.034737223538066 1101 70-71 1.0266956187466683 1101 72-73 0.9361211665000475 1101 74-75 1.0257673873823236 1101 76-77 1.186684299766224 1101 78-79 1.1492460605531782 1101 80-81 0.9721330888681656 1101 82-83 1.121506131025786 1101 84-85 1.213970206638141 1101 86-87 1.050021375520231 1101 88-89 1.0932940986711337 1101 90-91 1.134051281126542 1101 92-93 1.2487605723648443 1101 94-95 1.231898887988855 1101 96-97 1.1609166866982612 1101 98-99 1.1983884265865719 1101 100-101 1.4182070641372242 1102 1 0.4582865492734953 1102 2 0.5277534296875608 1102 3 0.4894445593046619 1102 4 0.37542212604041225 1102 5 0.42094255646341594 1102 6 0.4175521165813407 1102 7 0.46014883801267104 1102 8 0.5609729903947667 1102 9 0.6470979864048019 1102 10-11 0.6359900453250091 1102 12-13 0.580179086411384 1102 14-15 0.6788579206433738 1102 16-17 0.6723200452013245 1102 18-19 0.716398289228394 1102 20-21 0.7695186043652456 1102 22-23 0.7374434702764674 1102 24-25 0.8469158327843544 1102 26-27 0.9090222851948511 1102 28-29 0.8345830503005089 1102 30-31 0.8504040060970937 1102 32-33 1.0391526646293059 1102 34-35 0.9200772738760605 1102 36-37 0.9152025865244013 1102 38-39 0.9645603917458203 1102 40-41 0.9577858020339391 1102 42-43 0.9243534669740541 1102 44-45 1.0381608146607988 1102 46-47 0.9686572631033314 1102 48-49 0.8227008638909936 1102 50-51 0.9365335483824637 1102 52-53 1.0256869408211529 1102 54-55 0.8421336818782734 1102 56-57 0.8567704725292771 1102 58-59 0.9661315538984283 1102 60-61 0.9710481703373617 1102 62-63 0.8601909712252436 1102 64-65 0.8832758644146494 1102 66-67 0.9227829749463936 1102 68-69 0.8701898708162119 1102 70-71 0.876905754217713 1102 72-73 0.8471061279715819 1102 74-75 0.873413080894629 1102 76-77 1.0126969768728564 1102 78-79 1.097298477903415 1102 80-81 1.0081493081075372 1102 82-83 1.133449177259937 1102 84-85 1.0930799816816439 1102 86-87 1.1575061816350711 1102 88-89 1.1181814029216959 1102 90-91 1.1494002122724503 1102 92-93 1.118816376491118 1102 94-95 1.1058137835891557 1102 96-97 1.1440063585849103 1102 98-99 1.1642144581550546 1102 100-101 1.4554507877265834 1103 1 0.45603441810569123 1103 2 0.6003815511914645 1103 3 0.5129461953729937 1103 4 0.38618618902937385 1103 5 0.36786118777135357 1103 6 0.4251651586566041 1103 7 0.4767386476860125 1103 8 0.4932772116960962 1103 9 0.6237146621962992 1103 10-11 0.616098170539793 1103 12-13 0.6364046491607454 1103 14-15 0.6769993699386703 1103 16-17 0.7251676550152624 1103 18-19 0.6786945365176109 1103 20-21 0.7284059950029658 1103 22-23 0.674147631013831 1103 24-25 0.7607268010313106 1103 26-27 0.7384821394390286 1103 28-29 0.7541468336809416 1103 30-31 0.8497517365391616 1103 32-33 0.8660482418869435 1103 34-35 0.7887284650345094 1103 36-37 0.8355349703151518 1103 38-39 0.7439885620617446 1103 40-41 0.8624248886883237 1103 42-43 0.8283221807101881 1103 44-45 0.8485526189221488 1103 46-47 0.8071055576509778 1103 48-49 0.9109909216596108 1103 50-51 0.9454503981246916 1103 52-53 0.8161523398137263 1103 54-55 0.9764241039084354 1103 56-57 1.0600791633491227 1103 58-59 0.9586341417036834 1103 60-61 0.8494534764139843 1103 62-63 0.9608813736525761 1103 64-65 0.9935990831506629 1103 66-67 0.8346660572281834 1103 68-69 0.9677769488936363 1103 70-71 0.758185258275855 1103 72-73 0.6846510831258463 1103 74-75 0.7969908425073875 1103 76-77 0.9392625243861339 1103 78-79 0.9888590448592112 1103 80-81 0.9585466494370714 1103 82-83 0.9813868936545269 1103 84-85 0.8974987132590364 1103 86-87 0.9378562823373002 1103 88-89 0.8628741011233174 1103 90-91 0.9398427230098143 1103 92-93 1.006685290363862 1103 94-95 1.0995583411521714 1103 96-97 1.0169057744863927 1103 98-99 1.1667543535115357 1103 100-101 1.449854685520254 1104 1 0.41721746186903275 1104 2 0.5487464382995952 1104 3 0.466247257867348 1104 4 0.27192032995993287 1104 5 0.40122703981770513 1104 6 0.37594209622007924 1104 7 0.4390099174915889 1104 8 0.4519111070543005 1104 9 0.5473970788973404 1104 10-11 0.550409839092076 1104 12-13 0.5583477573477253 1104 14-15 0.6815318057319146 1104 16-17 0.576803830224442 1104 18-19 0.7358894640705245 1104 20-21 0.692652820887389 1104 22-23 0.6585772758044328 1104 24-25 0.7292385062925533 1104 26-27 0.7640378051608181 1104 28-29 0.6735131732128963 1104 30-31 0.8081455481867579 1104 32-33 0.8004504729893611 1104 34-35 0.7532091482697822 1104 36-37 0.8217434064129208 1104 38-39 0.665407416659427 1104 40-41 0.7670237488218561 1104 42-43 0.7473913959941001 1104 44-45 0.6777917813675813 1104 46-47 0.6968308246909913 1104 48-49 0.6930528382334771 1104 50-51 0.6884880590436353 1104 52-53 0.7799451725949496 1104 54-55 0.685702206184807 1104 56-57 0.6651754830688574 1104 58-59 0.6657516086198783 1104 60-61 0.7322824450986403 1104 62-63 0.7018701785987247 1104 64-65 0.6475972618666717 1104 66-67 0.6770506290759428 1104 68-69 0.6165928192059411 1104 70-71 0.6970643010571358 1104 72-73 0.5950531761270064 1104 74-75 0.7834107387662002 1104 76-77 0.9220068027746464 1104 78-79 0.939099099061 1104 80-81 0.7966761093254462 1104 82-83 0.8536022501593585 1104 84-85 0.9194595267344106 1104 86-87 0.9028834392988117 1104 88-89 1.0117930457228788 1104 90-91 1.0103193316680574 1104 92-93 0.9476131596091264 1104 94-95 1.019935741177786 1104 96-97 0.879226855169982 1104 98-99 1.115997481941566 1104 100-101 1.4526902169651414 1105 1 0.12615477871500502 1105 2 0.26646378298240947 1105 3 0.3142682091745783 1105 4 0.1602221740793297 1105 5 0.12873057082460093 1105 6 0.20800723694348733 1105 7 0.28121614428589226 1105 8 0.20871814016894774 1105 9 0.25671431095345554 1105 10-11 0.3080690827912278 1105 12-13 0.38133531186915803 1105 14-15 0.39828614074608026 1105 16-17 0.31943312104747434 1105 18-19 0.370128808368392 1105 20-21 0.3757013320389788 1105 22-23 0.49677814426364364 1105 24-25 0.5123882215560442 1105 26-27 0.45144417565125394 1105 28-29 0.5543090361807046 1105 30-31 0.5014112483884219 1105 32-33 0.5233948501357304 1105 34-35 0.48940538144359635 1105 36-37 0.43212907860640826 1105 38-39 0.3862458687391097 1105 40-41 0.39761440503228584 1105 42-43 0.48601124490755865 1105 44-45 0.47172881550454804 1105 46-47 0.3944379928206061 1105 48-49 0.37084967269221636 1105 50-51 0.45539628484760186 1105 52-53 0.38480122802190664 1105 54-55 0.46800864903732275 1105 56-57 0.5785020713651576 1105 58-59 0.4163722262733671 1105 60-61 0.4023060384500141 1105 62-63 0.43869790943919185 1105 64-65 0.4244180934651993 1105 66-67 0.4938746133082077 1105 68-69 0.4825464309419587 1105 70-71 0.43405009193129374 1105 72-73 0.39864252388020915 1105 74-75 0.566308500339133 1105 76-77 0.6535286281952679 1105 78-79 0.59916128361915 1105 80-81 0.6336253764808824 1105 82-83 0.5917763612108828 1105 84-85 0.6322854059736933 1105 86-87 0.53589696878079 1105 88-89 0.6165295637874131 1105 90-91 0.6106121496600032 1105 92-93 0.718485536769208 1105 94-95 0.5218130431858192 1105 96-97 0.4495511195411659 1105 98-99 0.823792140397682 1105 100-101 1.1296135662729618 1106 1 0.05525412684571762 1106 2 0.1346115607129228 1106 3 0.12342777262412241 1106 4 0.04401455794791076 1106 5 0.11351881741600778 1106 6 0.11264197656252861 1106 7 0.06069604443390375 1106 8 0.0567089588233074 1106 9 0.12690192252300392 1106 10-11 0.11510449152891766 1106 12-13 0.048807339166231145 1106 14-15 0.135565216430777 1106 16-17 0.10424991001708861 1106 18-19 0.16585896795450594 1106 20-21 0.06835376022890927 1106 22-23 0.13448177735725864 1106 24-25 0.10474743305176304 1106 26-27 0.07155516994311739 1106 28-29 0.1586958484296872 1106 30-31 0.16010452662207086 1106 32-33 0.2375691972309184 1106 34-35 0.18500094582516624 1106 36-37 0.27630190061305626 1106 38-39 0.16791079874748505 1106 40-41 0.21733520664830053 1106 42-43 0.2909672970137791 1106 44-45 0.18044106244563096 1106 46-47 0.05775206046732251 1106 48-49 0.2029095124328748 1106 50-51 0.026032251236259185 1106 52-53 -0.02023987624045276 1106 54-55 0.13514531874188407 1106 56-57 0.1900082894503683 1106 58-59 0.23527689339042013 1106 60-61 0.036811088603208475 1106 62-63 -0.0045389313410098 1106 64-65 0.024661906668868028 1106 66-67 -0.1931863166586325 1106 68-69 -0.013425091211402673 1106 70-71 -0.11925736290470113 1106 72-73 -0.026117718468196216 1106 74-75 0.20882646171777708 1106 76-77 0.32324346231620993 1106 78-79 0.2323030765494174 1106 80-81 0.16818113638639787 1106 82-83 0.22885954786888263 1106 84-85 0.26410996787079455 1106 86-87 0.3258260979384886 1106 88-89 0.44701678156927827 1106 90-91 0.2556588440529133 1106 92-93 0.42513707201198514 1106 94-95 0.3664980705341421 1106 96-97 0.36802813106429255 1106 98-99 0.24838739741696614 1106 100-101 0.48677104908194124 1107 1 0.12862655825570002 1107 2 0.18481629143420975 1107 3 0.2046130176788843 1107 4 -0.03192094747867458 1107 5 0.13007362124711364 1107 6 0.19968496553484272 1107 7 0.1882973157186143 1107 8 0.15423433989185753 1107 9 0.09733300668838751 1107 10-11 0.13436802497446365 1107 12-13 0.1629268411236282 1107 14-15 0.13279950196684354 1107 16-17 0.18803530656694534 1107 18-19 0.22420863362680166 1107 20-21 0.2824099235675632 1107 22-23 0.29628084297162616 1107 24-25 0.2055403602282837 1107 26-27 0.2767130702330647 1107 28-29 0.34171061405854886 1107 30-31 0.2525497954528646 1107 32-33 0.29804704381079006 1107 34-35 0.18850937112225807 1107 36-37 0.2616259378677057 1107 38-39 0.3656026883849961 1107 40-41 0.26920950221769147 1107 42-43 0.47454494355774557 1107 44-45 0.39668612417342075 1107 46-47 0.18918077266945232 1107 48-49 0.30972408754819725 1107 50-51 0.4233751646600936 1107 52-53 0.22991241514772298 1107 54-55 0.40787713095300404 1107 56-57 0.36910943183485045 1107 58-59 0.3559969330459012 1107 60-61 0.25522182345394384 1107 62-63 0.12351606210781085 1107 64-65 0.1765461891027016 1107 66-67 0.2911348038343746 1107 68-69 0.2926234482677259 1107 70-71 0.15939349133717684 1107 72-73 0.22548764510841934 1107 74-75 0.3874164823517674 1107 76-77 0.3973805856148367 1107 78-79 0.5397509383152865 1107 80-81 0.4509855806406158 1107 82-83 0.48242379675216185 1107 84-85 0.4844909518191507 1107 86-87 0.6299613731695004 1107 88-89 0.6236582853189958 1107 90-91 0.5431668194434174 1107 92-93 0.5671623119040987 1107 94-95 0.6313331263527502 1107 96-97 0.632011068456606 1107 98-99 0.625639543182718 1107 100-101 0.9581663965302631 1108 1 0.2802867798046833 1108 2 0.358300182184923 1108 3 0.33120862188263445 1108 4 0.1303487929928977 1108 5 0.2488495629011851 1108 6 0.2666660006265005 1108 7 0.17224877328120414 1108 8 0.28722177711898667 1108 9 0.31261533563354504 1108 10-11 0.270529444486705 1108 12-13 0.38219953761994674 1108 14-15 0.4909969981859632 1108 16-17 0.4778391702698741 1108 18-19 0.49526233393082464 1108 20-21 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-0.4478094336672882 2316 76-77 -0.8849873377445299 2316 78-79 -0.7692042190567996 2316 80-81 -0.8079474244670806 2316 82-83 -0.9184503143427243 2316 84-85 -0.9150353497140209 2316 86-87 -0.8446811266306149 2316 88-89 -1.05712040520039 2316 90-91 -0.7219743139408514 2316 92-93 -0.9574251173397741 2316 94-95 -0.8399604108353458 2316 96-97 -0.8052181713459063 2316 98-99 -0.6835120250910869 2316 100-101 -0.8406620210362767 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 30.0 4 79.0 5 165.0 6 345.0 7 877.0 8 2024.0 9 3408.0 10 5527.0 11 8646.0 12 11814.0 13 15226.0 14 18339.0 15 22665.0 16 26973.0 17 30880.0 18 27774.0 19 23278.0 20 23228.0 21 25174.0 22 27631.0 23 32158.0 24 39397.0 25 49655.0 26 63264.0 27 79728.0 28 100102.0 29 123792.0 30 150519.0 31 179494.0 32 214940.0 33 258127.0 34 317994.0 35 394194.0 36 505763.0 37 626631.0 38 484965.0 39 79091.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.51156618494469 20.900453214140725 14.207547260039007 23.380433340875577 2 15.911479861139947 21.70160187327699 39.23984312185989 23.147075143723175 3 18.93457561041407 26.694571261907434 29.97097472480848 24.399878402870016 4 13.131385090028132 17.408451427501223 37.81163971830589 31.648523764164754 5 13.152702231717644 38.49395233080304 34.69569145248494 13.657653984994381 6 30.86415559068465 37.752804917267966 17.610018737194615 13.773020754852771 7 27.007264370963053 32.807915416430546 22.60538471371816 17.579435498888248 8 25.957418324896654 35.60080283528892 20.399273663560074 18.042505176254355 9 26.572032869349243 15.565657544260509 20.529855318999022 37.33245426739123 10-11 24.95079161210252 26.587653085772324 28.2825429626595 20.179012339465654 12-13 25.397013927823338 24.08660625941766 28.70523684949556 21.811142963263443 14-15 22.597423120848948 25.423565246920827 26.320149484809125 25.658862147421104 16-17 22.161125197412325 28.389981066534876 26.964724468257508 22.48416926779529 18-19 22.078083683701415 27.277338021236485 29.106239286386526 21.53833900867557 20-21 22.922766862711743 26.446306363798328 28.95540570057344 21.67552107291649 22-23 22.67922356116296 26.3203203392123 28.866287317599472 22.13416878202527 24-25 22.39021706266529 26.5773396074409 28.946209874258237 22.08623345563557 26-27 22.304068608700902 26.714210939544618 28.806481891570872 22.1752385601836 28-29 22.241005840089063 26.917795361346702 28.824392689118426 22.016806109445806 30-31 22.616635591021524 26.645211930531147 28.667955829885823 22.070196648561506 32-33 22.461773044120527 26.80963031974839 28.48405159356957 22.244545042561512 34-35 22.545868849500796 26.80558161448233 28.682717791072132 21.965831744944747 36-37 22.594403114933506 26.617741461827443 28.64947913743942 22.13837628579963 38-39 22.51174258576073 26.714085352880257 28.616276668921802 22.15789539243721 40-41 22.449577894360033 26.651521738173084 28.74348471341547 22.155415654051417 42-43 22.489213389351942 26.751612313440305 28.318355791990584 22.440818505217173 44-45 22.45091456524617 26.90401255089237 28.314905795325224 22.33016708853624 46-47 22.609586643416495 26.865963306238616 28.079739459848785 22.444710590496104 48-49 22.612099383505768 26.91926659924528 27.929832429285245 22.538801587963707 50-51 22.635884195376907 27.009082287931307 27.68380291073405 22.671230605957735 52-53 22.66974475832537 27.03707997713461 27.6923011076573 22.600874156882725 54-55 22.50568244596737 26.98443899515781 27.802721281908465 22.707157276966356 56-57 22.59415605374259 27.14313451641394 27.591782199883653 22.67092722995981 58-59 22.5715391722792 27.05635872012379 27.733877022569008 22.638225085028 60-61 22.62440009753785 27.252978913923354 27.71381629675173 22.40880469178707 62-63 22.518312795890992 27.07379512363207 27.711448043018493 22.69644403745844 64-65 22.302011304535544 27.054221114780656 27.72310260859752 22.92066497208628 66-67 22.43117630981335 27.102206816810654 27.59560122879429 22.871015644581703 68-69 22.374336572648563 27.082962567199196 27.68628959420206 22.856411265950186 70-71 22.362274534744238 26.862493348991407 27.75932321855293 23.015908897711423 72-73 22.50982973543444 27.068940856394452 27.53830537224567 22.882924035925438 74-75 22.35713388453267 27.279498825308757 27.412997932119648 22.95036935803893 76-77 22.289690775114575 27.169521195496433 27.43306663752685 23.10772139186214 78-79 22.247669221391032 27.28560882228629 27.357520607476538 23.109201348846145 80-81 22.32281665415999 27.400866357749283 27.23352352538251 23.042793462708218 82-83 22.50042527320658 27.17515921322947 27.423289772607173 22.90112574095678 84-85 22.4868492439196 27.239818355496194 27.033497940067182 23.23983446051703 86-87 22.164585260483612 27.44116886215008 27.237629079225627 23.156616798140675 88-89 22.253692774545165 27.22573667388961 27.15237062549746 23.36819992606776 90-91 22.288691969658153 27.17336486770111 27.29726845870325 23.240674703937483 92-93 22.377803047234757 27.184574758329354 27.152107029741444 23.28551516469444 94-95 22.14201026637541 27.090062487315503 27.250662550755294 23.51726469555379 96-97 22.10344909152584 26.981433427379947 27.217082595605945 23.69803488548827 98-99 21.88712594830892 27.134481453558656 27.13848254467382 23.839910053458606 100-101 21.904677904616 27.114954115789057 27.082794402297385 23.897573577297557 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1054.0 1 774.0 2 607.0 3 781.0 4 690.0 5 654.0 6 1077.0 7 2099.5 8 3200.5 9 3575.0 10 3129.0 11 2628.0 12 2680.0 13 2989.0 14 3343.5 15 3713.5 16 4091.0 17 4425.5 18 4762.0 19 5278.0 20 5936.0 21 6718.5 22 7943.5 23 9592.0 24 11589.5 25 14621.0 26 19513.0 27 25680.5 28 32877.5 29 43115.5 30 54679.0 31 65120.5 32 76263.5 33 83321.0 34 88369.0 35 94964.5 36 102279.5 37 109698.0 38 111326.5 39 109298.0 40 108461.5 41 109392.0 42 111370.0 43 115543.0 44 121295.0 45 128157.0 46 134159.0 47 138466.5 48 143263.0 49 145855.5 50 144896.5 51 143972.0 52 145201.5 53 146520.0 54 144788.0 55 136112.0 56 124141.0 57 110905.0 58 97007.0 59 85251.0 60 72771.5 61 59279.5 62 47494.5 63 36915.0 64 27529.0 65 20268.5 66 14419.0 67 10775.5 68 7802.0 69 5165.0 70 3747.5 71 2710.5 72 1910.0 73 1454.0 74 1080.0 75 737.5 76 488.5 77 281.5 78 168.5 79 97.0 80 52.0 81 33.5 82 17.5 83 12.5 84 7.5 85 3.0 86 2.0 87 3.5 88 2.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0185962662522308 2 0.003296496453372442 3 0.0034726451188198237 4 0.03472645118819824 5 0.0017866507495377356 6 5.032819012782354E-5 7 0.0 8 0.0 9 2.516409506391177E-5 10-11 0.0 12-13 0.0 14-15 1.2582047531955886E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.807433272369119E-5 24-25 4.40371663618456E-4 26-27 0.0019124712248572945 28-29 1.0065638025564709E-4 30-31 0.007020782522831384 32-33 0.02268543170011646 34-35 0.013827670237619516 36-37 6.794305667256177E-4 38-39 6.416844241297501E-4 40-41 0.0022270224131561915 42-43 0.008945835795220633 44-45 0.00840480775134653 46-47 0.008178330895771326 48-49 0.010053055978032752 50-51 0.18771156712924983 52-53 0.04968650570369379 54-55 0.017992327970696914 56-57 0.010556337879310988 58-59 0.02045840928696027 60-61 0.053461119963280554 62-63 0.09586262014597188 64-65 0.10078220073096664 66-67 0.10495944051157598 68-69 0.10305955133425065 70-71 0.09193702131600164 72-73 0.07050979436908078 74-75 0.23830398025524446 76-77 0.28716007082182915 78-79 0.007486318281513752 80-81 9.562356124286472E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.7614866544738239E-4 90-91 0.006592992906744884 92-93 0.018621430347294708 94-95 0.001748904606941868 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3973916.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.31879736345459 #Duplication Level Percentage of deduplicated Percentage of total 1 73.32445987299027 36.16274178260765 2 14.110061404966496 13.917825184348878 3 4.934066147024796 7.300266254489912 4 2.344179653500796 4.624484852581558 5 1.291565455039334 3.1849227479361466 6 0.8351481188536939 2.4713100505329373 7 0.5572142540067558 1.9236795816971395 8 0.40650912064578604 1.6038832758020507 9 0.2951037260231995 1.309874477844474 >10 1.7051588610326234 15.404061386927514 >50 0.1281513801875586 4.359975933750883 >100 0.06444863827377692 5.560015383753393 >500 0.003013879000593098 0.9958435477319431 >1k 7.662403786255948E-4 0.5295394295287447 >5k 1.0216538381674523E-4 0.3718289376475903 >10k+ 5.1082691908372614E-5 0.2797471728192183 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11104 0.2794221115896763 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8488 0.2135928389024831 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6271 0.1578040401457907 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.54922851917353E-5 2 0.0 0.0 0.0 0.0 7.54922851917353E-5 3 0.0 0.0 0.0 0.0 7.54922851917353E-5 4 0.0 0.0 0.0 0.0 7.54922851917353E-5 5 0.0 0.0 0.0 0.0 7.54922851917353E-5 6 0.0 0.0 0.0 0.0 7.54922851917353E-5 7 0.0 0.0 0.0 0.0 7.54922851917353E-5 8 0.0 0.0 0.0 0.0 7.54922851917353E-5 9 0.0 0.0 0.0 0.0 7.54922851917353E-5 10-11 0.0 0.0 0.0 0.0 1.2582047531955884E-4 12-13 0.0 0.0 0.0 0.0 3.397152833628089E-4 14-15 1.2582047531955884E-5 0.0 0.0 0.0 4.277896160865001E-4 16-17 2.5164095063911768E-5 0.0 0.0 6.291023765977942E-5 5.913562340019265E-4 18-19 2.5164095063911768E-5 0.0 0.0 7.54922851917353E-5 7.800869469812648E-4 20-21 2.5164095063911768E-5 0.0 0.0 1.2582047531955884E-4 8.933253747688678E-4 22-23 2.5164095063911768E-5 0.0 0.0 2.390589031071618E-4 0.0010568919926842943 24-25 2.5164095063911768E-5 0.0 0.0 4.1520756855454417E-4 0.0010568919926842943 26-27 2.5164095063911768E-5 0.0 0.0 9.939817550245148E-4 0.0011575483729399415 28-29 2.5164095063911768E-5 0.0 0.0 0.0025541556489870445 0.0011827124680038532 30-31 2.5164095063911768E-5 0.0 0.0 0.008769687129773251 0.0012078765630677648 32-33 2.5164095063911768E-5 1.2582047531955884E-5 0.0 0.02463564906756962 0.0012330406581316767 34-35 2.5164095063911768E-5 2.5164095063911768E-5 0.0 0.04841571890296624 0.0012833688482595002 36-37 2.5164095063911768E-5 2.5164095063911768E-5 0.0 0.08390967499061379 0.0012833688482595002 38-39 2.5164095063911768E-5 2.5164095063911768E-5 0.0 0.13730788471623456 0.0013588611334512356 40-41 2.5164095063911768E-5 2.5164095063911768E-5 0.0 0.19644350811642722 0.0013588611334512356 42-43 2.5164095063911768E-5 2.5164095063911768E-5 0.0 0.2559817570376425 0.0013966072760471032 44-45 2.5164095063911768E-5 2.5164095063911768E-5 0.0 0.3283788585365166 0.0014972636563027504 46-47 2.5164095063911768E-5 2.5164095063911768E-5 0.0 0.4123640258123221 0.0016105020840903534 48-49 2.5164095063911768E-5 2.5164095063911768E-5 0.0 0.5018978760497202 0.0016734123217501326 50-51 5.0328190127823536E-5 2.5164095063911768E-5 0.0 0.5953195789744927 0.001736322559409912 52-53 5.0328190127823536E-5 2.5164095063911768E-5 0.0 0.693056924202726 0.0019250532723892504 54-55 5.0328190127823536E-5 2.5164095063911768E-5 0.0 0.7955880295406345 0.0019879635100490296 56-57 5.0328190127823536E-5 2.5164095063911768E-5 0.0 0.917797457218522 0.0020131276051129415 58-59 5.0328190127823536E-5 2.5164095063911768E-5 0.0 1.0524631119530459 0.0020382917001768534 60-61 5.0328190127823536E-5 2.5164095063911768E-5 0.0 1.1979996557551795 0.0020760378427727207 62-63 5.0328190127823536E-5 2.5164095063911768E-5 0.0 1.3497894771806953 0.0022647685557520593 64-65 5.0328190127823536E-5 5.0328190127823536E-5 0.0 1.507304130233251 0.002289932650815971 66-67 5.0328190127823536E-5 5.0328190127823536E-5 0.0 1.6834527956806333 0.0023402608409437945 68-69 5.0328190127823536E-5 5.0328190127823536E-5 2.5164095063911768E-5 1.8821736544003447 0.0023654249360077063 70-71 5.0328190127823536E-5 5.0328190127823536E-5 2.5164095063911768E-5 2.105215610999327 0.0024157531261355296 72-73 6.291023765977942E-5 5.0328190127823536E-5 2.5164095063911768E-5 2.3369391803953583 0.002516409506391177 74-75 7.54922851917353E-5 5.0328190127823536E-5 2.5164095063911768E-5 2.5770172293526086 0.0026170658866468237 76-77 7.54922851917353E-5 5.0328190127823536E-5 2.5164095063911768E-5 2.8402336637211256 0.0026673940767746474 78-79 7.54922851917353E-5 5.0328190127823536E-5 2.5164095063911768E-5 3.1364653908134947 0.0027051402193705152 80-81 7.54922851917353E-5 5.0328190127823536E-5 2.5164095063911768E-5 3.462604644889323 0.002768050457030295 82-83 7.54922851917353E-5 5.0328190127823536E-5 2.5164095063911768E-5 3.809240054394708 0.002944199122477677 84-85 7.54922851917353E-5 5.0328190127823536E-5 2.5164095063911768E-5 4.16653245815966 0.0030196914076694122 86-87 7.54922851917353E-5 5.0328190127823536E-5 2.5164095063911768E-5 4.547516857427283 0.003170675978052883 88-89 7.54922851917353E-5 5.0328190127823536E-5 2.5164095063911768E-5 4.959830555049478 0.0033216605484363533 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5580 0.0 55.567772 1 GTATCAA 11035 0.0 44.579113 1 CAACGCA 14485 0.0 33.417942 5 ATCAACG 14495 0.0 33.407497 3 TCAACGC 14565 0.0 33.278698 4 TATCAAC 14730 0.0 33.19724 2 AACGCAG 15360 0.0 31.699629 6 GTGGTAT 2245 0.0 30.034197 1 ACGCAGA 18170 0.0 26.536085 7 GTACATG 17700 0.0 26.156242 1 CGCAGAG 18560 0.0 25.901775 8 TACATGG 18000 0.0 25.504772 2 ACATGGG 18095 0.0 24.688404 3 TGGTATC 2630 0.0 23.827894 2 GCAGAGT 20465 0.0 23.026903 9 CATGGGG 11610 0.0 22.20336 4 AGAGTAC 19825 0.0 20.287313 10-11 CATGGGA 6960 0.0 19.780617 4 GAGTACA 13115 0.0 19.623432 1 AGTACTT 13145 0.0 19.170435 12-13 >>END_MODULE