FastQCFastQC Report
Sat 18 Jun 2016
SRR1930947_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930947_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3244381
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN59610.1837330449167345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27500.054.144711
GTATCAA53700.048.3361931
ATCAACG67300.038.3976363
TCAACGC67550.038.3917854
TATCAAC69350.037.270242
CAACGCA69350.036.4934355
AACGCAG75800.033.999016
ACGCAGA88150.028.8347557
CGCAGAG90100.028.0289698
GTACATG67650.024.7613031
GCAGAGT96600.024.3928079
TACATGG70250.023.7649732
ACATGGG69550.023.4932293
GAGTACA51400.020.8455581
AGAGTAC88950.020.16358610-11
CAGAGTA92500.019.82062510-11
GAGTACT63000.019.72669412-13
CATGGGG53700.018.718444
AGTACAT53850.018.6762562
GTACTTT70050.018.18105314-15
Produced by FastQC (version 0.11.5)