FastQCFastQC Report
Sat 18 Jun 2016
SRR1930947_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930947_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3244381
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64330.19828127460985623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53570.16511624251282447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36130.11136176669756112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38050.056.277641
GTATCAA74500.042.7004551
ATCAACG95050.033.3125083
CAACGCA94950.033.1883965
TCAACGC95450.033.072424
TATCAAC96200.032.91532
AACGCAG100800.031.4972066
ACGCAGA117400.026.5989327
CGCAGAG118900.026.0638038
GTACATG94350.024.5076161
TACATGG99400.023.1156432
ACATGGG95350.022.9516473
GCAGAGT135150.022.3684839
CATGGGG68000.020.0351774
GTGGTAT18650.019.8577371
AGAGTAC125950.019.66914410-11
CAGAGTA130900.018.92535210-11
TGGTATC18650.018.581942
GAGTACT89700.017.75060812-13
GAGTACA71200.017.4717041
Produced by FastQC (version 0.11.5)