FastQCFastQC Report
Sat 18 Jun 2016
SRR1930946_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930946_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3298161
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN58890.17855404875626144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23900.052.313911
GTATCAA46950.046.0664861
TCAACGC58400.037.3418354
ATCAACG58000.037.0942343
TATCAAC59850.036.2932552
CAACGCA59450.036.2696765
AACGCAG63950.033.687236
ACGCAGA75100.028.0964667
GTACATG62750.026.7543181
CGCAGAG79900.026.447578
TACATGG65350.025.6038512
ACATGGG66050.024.8685993
GCAGAGT85100.023.3614779
CATGGGG48500.022.4301724
AGAGTAC76100.019.80910110-11
GAGTACT53400.019.19322212-13
GAGTACA45250.019.0520151
CAGAGTA82350.018.73513810-11
GTGGTAT15350.018.0641561
TGGTATC14550.017.6652582
Produced by FastQC (version 0.11.5)