##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930946_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3298161 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.849765672445947 30.0 10.0 31.0 2.0 33.0 2 21.714341113123343 30.0 10.0 31.0 2.0 33.0 3 22.17436959566255 28.0 10.0 31.0 2.0 33.0 4 24.099304733759208 32.0 2.0 35.0 2.0 37.0 5 24.07685404078212 32.0 10.0 35.0 2.0 37.0 6 24.84530409522155 32.0 11.0 35.0 2.0 37.0 7 24.93283651101326 32.0 11.0 35.0 2.0 37.0 8 25.735434989377413 33.0 17.0 35.0 2.0 37.0 9 26.920924418183347 33.0 17.0 37.0 2.0 39.0 10-11 27.191972283948537 33.5 17.5 37.0 2.0 39.0 12-13 27.131420812992452 34.0 17.5 37.0 2.0 39.0 14-15 27.344660857975096 34.0 17.0 38.0 2.0 40.0 16-17 26.428629924373006 33.0 13.5 38.0 2.0 40.0 18-19 25.785090236650063 32.5 10.0 38.0 2.0 40.0 20-21 25.27842136875671 32.0 10.0 38.0 2.0 40.0 22-23 25.21500087472989 32.0 10.0 38.0 2.0 40.0 24-25 24.908193232531705 32.0 9.5 38.0 2.0 40.0 26-27 24.57468267922639 31.5 8.0 37.5 2.0 40.0 28-29 24.509287903167856 31.0 5.0 38.0 2.0 40.0 30-31 24.426388523786436 31.0 8.0 37.0 2.0 39.0 32-33 24.13467277673831 31.0 2.0 37.0 2.0 39.0 34-35 21.234465509718902 25.0 2.0 36.0 2.0 39.0 36-37 19.57845341691931 18.0 2.0 35.0 2.0 38.5 38-39 18.668777236769216 18.0 2.0 34.0 2.0 38.0 40-41 20.010409588858764 23.5 2.0 35.0 2.0 38.0 42-43 19.921870551498245 23.5 2.0 35.0 2.0 38.0 44-45 17.938307893398775 17.5 2.0 34.0 2.0 38.0 46-47 18.78939187626074 19.0 2.0 33.0 2.0 38.0 48-49 18.220687983394384 17.5 2.0 33.0 2.0 38.0 50-51 16.23892587414623 11.0 2.0 31.5 2.0 37.5 52-53 19.175468238209113 22.0 2.0 33.5 2.0 37.5 54-55 20.790638176850678 26.5 2.0 35.5 2.0 38.5 56-57 21.00352878467728 28.0 2.0 36.0 2.0 39.0 58-59 20.623519743275118 26.5 2.0 36.0 2.0 39.0 60-61 20.29936819336594 26.0 2.0 35.0 2.0 39.0 62-63 19.815025100351377 25.5 2.0 35.0 2.0 38.0 64-65 19.316077656609245 24.5 2.0 34.0 2.0 38.0 66-67 18.89047593492252 23.0 2.0 34.0 2.0 38.0 68-69 18.427415156506914 22.0 2.0 34.0 2.0 37.0 70-71 17.8299623638749 20.0 2.0 33.0 2.0 36.5 72-73 17.347962546400858 18.0 2.0 32.5 2.0 36.0 74-75 16.802989150620604 14.0 2.0 32.0 2.0 35.5 76-77 16.235514882384457 9.5 2.0 31.5 2.0 35.0 78-79 15.73713260207734 7.0 2.0 31.0 2.0 35.0 80-81 15.225290851477535 2.0 2.0 31.0 2.0 34.0 82-83 14.74072111701036 2.0 2.0 30.5 2.0 34.0 84-85 14.213745932960823 2.0 2.0 30.0 2.0 34.0 86-87 13.650414579518706 2.0 2.0 29.5 2.0 34.0 88-89 13.093373549684202 2.0 2.0 29.0 2.0 34.0 90-91 12.512161322627975 2.0 2.0 29.0 2.0 34.0 92-93 11.884825058570518 2.0 2.0 27.5 2.0 34.0 94-95 11.226361448091831 2.0 2.0 26.0 2.0 33.0 96-97 10.495478995719129 2.0 2.0 25.0 2.0 33.0 98-99 9.700352560108497 2.0 2.0 22.0 2.0 32.0 100-101 8.656522225567521 2.0 2.0 9.5 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.615339798466639 1101 2 7.744088917871604 1101 3 7.561841958216753 1101 4 9.299179456705726 1101 5 9.269619716363128 1101 6 8.509181287749986 1101 7 8.300493945309402 1101 8 7.417969779745135 1101 9 7.551271720110282 1101 10-11 6.812424389942276 1101 12-13 6.5790806073567545 1101 14-15 7.245780843565786 1101 16-17 8.018687242879064 1101 18-19 8.474654007179804 1101 20-21 8.8105056661088 1101 22-23 8.909967375106472 1101 24-25 9.020100901976935 1101 26-27 8.862721114486622 1101 28-29 8.860059435273516 1101 30-31 8.67661271959754 1101 32-33 8.66236457215982 1101 34-35 11.186308918679856 1101 36-37 5.4217041348110335 1101 38-39 8.492623864181056 1101 40-41 9.790152612537248 1101 42-43 10.29281548529774 1101 44-45 4.589070103739381 1101 46-47 6.220283672628863 1101 48-49 9.304277067867762 1101 50-51 -3.359966973790808 1101 52-53 6.101685482343601 1101 54-55 8.506683536531302 1101 56-57 9.059304033076877 1101 58-59 9.127119255444583 1101 60-61 8.92158312290432 1101 62-63 8.941799146583065 1101 64-65 9.05282418318448 1101 66-67 8.879964073709903 1101 68-69 9.120281222893745 1101 70-71 9.0212289451899 1101 72-73 8.98906803122074 1101 74-75 8.883524454733987 1101 76-77 8.912381787333242 1101 78-79 8.654617185025401 1101 80-81 8.432995977481545 1101 82-83 8.148439551117301 1101 84-85 7.967442596666359 1101 86-87 7.841162162318595 1101 88-89 7.738032170932398 1101 90-91 7.631506965687118 1101 92-93 7.515323403362231 1101 94-95 7.283143779920989 1101 96-97 7.157321764503125 1101 98-99 6.675347762350741 1101 100-101 6.0122770932365235 1102 1 6.453806908230213 1102 2 7.608253875616079 1102 3 7.39825799078497 1102 4 9.042066647089417 1102 5 8.96240327824859 1102 6 8.253360545597257 1102 7 8.039701332744723 1102 8 7.113849782650362 1102 9 6.956316202731156 1102 10-11 6.5728713116959945 1102 12-13 6.453762106751629 1102 14-15 6.790244148738655 1102 16-17 7.5995285884449935 1102 18-19 7.818345203148656 1102 20-21 8.408119848297819 1102 22-23 8.144357417728866 1102 24-25 8.169854781977623 1102 26-27 8.266714674808302 1102 28-29 8.099542013531288 1102 30-31 7.8192780619884665 1102 32-33 7.809026828485553 1102 34-35 10.125760962382813 1102 36-37 0.8875449912311808 1102 38-39 3.3088199719887896 1102 40-41 7.956877118222085 1102 42-43 9.07100774515866 1102 44-45 0.8845129974517079 1102 46-47 1.4738762538437378 1102 48-49 7.051792892845469 1102 50-51 -4.769157152188203 1102 52-53 4.2960576563223825 1102 54-55 7.095766803224695 1102 56-57 7.619551183246859 1102 58-59 7.938035738619821 1102 60-61 7.7070721315762825 1102 62-63 7.650403146757974 1102 64-65 7.704103216024688 1102 66-67 7.762413865860001 1102 68-69 7.7929226786657 1102 70-71 7.687159144828083 1102 72-73 7.532188501526996 1102 74-75 7.408575018326275 1102 76-77 7.334785016140071 1102 78-79 7.300007523358644 1102 80-81 7.3655152598305715 1102 82-83 7.199830753978658 1102 84-85 7.057535838228038 1102 86-87 6.786855400471074 1102 88-89 6.65676069205942 1102 90-91 6.6274165304140755 1102 92-93 6.669553754709714 1102 94-95 6.254525324005995 1102 96-97 6.098800103868021 1102 98-99 5.681662004423059 1102 100-101 5.198839934387914 1103 1 6.363110463901386 1103 2 7.433742925293966 1103 3 7.17345016012618 1103 4 8.80263100973934 1103 5 8.871481017472664 1103 6 8.210790047842263 1103 7 7.749653127307123 1103 8 7.01082230342935 1103 9 7.121632963565407 1103 10-11 6.376576904959965 1103 12-13 6.296460236326741 1103 14-15 6.724837097747571 1103 16-17 7.420667029315364 1103 18-19 7.87432050210732 1103 20-21 8.268552946032354 1103 22-23 8.130981435438695 1103 24-25 8.175226663878167 1103 26-27 8.190838345370882 1103 28-29 8.049322393378223 1103 30-31 7.716294516814113 1103 32-33 7.540821128641685 1103 34-35 9.712769769497413 1103 36-37 -3.359165304751354 1103 38-39 -1.9795737182261952 1103 40-41 6.094446819700938 1103 42-43 8.558423076511062 1103 44-45 0.6457156102572661 1103 46-47 0.4516156236027804 1103 48-49 6.986264966316671 1103 50-51 -5.129465641129251 1103 52-53 4.1480938878428795 1103 54-55 6.992976658910688 1103 56-57 7.541092078195682 1103 58-59 7.737881782793707 1103 60-61 7.76193346343258 1103 62-63 7.609016773997482 1103 64-65 7.625095504484076 1103 66-67 7.468924235979532 1103 68-69 7.442745062192902 1103 70-71 7.604826650323936 1103 72-73 7.34579291354331 1103 74-75 7.196437721197995 1103 76-77 7.023831898639308 1103 78-79 6.906051109028802 1103 80-81 6.726836007417772 1103 82-83 6.588002716116888 1103 84-85 6.52593979216476 1103 86-87 6.2853061448504075 1103 88-89 6.193451630655465 1103 90-91 6.24697732470886 1103 92-93 6.058790332130016 1103 94-95 5.828925515003002 1103 96-97 5.595935421915462 1103 98-99 5.465150606292365 1103 100-101 4.94473747327091 1104 1 6.32228043512394 1104 2 7.477439765848732 1104 3 6.9202510706339275 1104 4 8.864257658177802 1104 5 8.706454177554772 1104 6 7.917958066439748 1104 7 7.698097369987249 1104 8 6.733689066616641 1104 9 6.904952231820378 1104 10-11 6.211965813487328 1104 12-13 5.942749762797806 1104 14-15 6.415603942132268 1104 16-17 7.140043596669212 1104 18-19 7.576704615319311 1104 20-21 7.836154996809288 1104 22-23 7.800161681717267 1104 24-25 7.936925147470095 1104 26-27 7.952459475370208 1104 28-29 7.765515229160062 1104 30-31 7.405142185160781 1104 32-33 7.348751897913836 1104 34-35 9.940485861075043 1104 36-37 -3.3025057287583692 1104 38-39 -2.0901758198491187 1104 40-41 5.811089850788665 1104 42-43 7.8900691644322904 1104 44-45 0.22677748983416635 1104 46-47 -0.17837407357547264 1104 48-49 6.400019137900909 1104 50-51 -5.396565340409026 1104 52-53 3.6253650335732317 1104 54-55 6.303640790913139 1104 56-57 6.828630436119909 1104 58-59 7.222996834440568 1104 60-61 7.155336525194503 1104 62-63 7.138137950417644 1104 64-65 7.134656677509877 1104 66-67 6.951404840314563 1104 68-69 6.921968225615576 1104 70-71 6.811568595094773 1104 72-73 6.554528394451854 1104 74-75 6.580509111058539 1104 76-77 6.453704164126901 1104 78-79 6.3290091867655605 1104 80-81 6.0744300807529665 1104 82-83 5.846018769568696 1104 84-85 5.689411751118676 1104 86-87 5.71161788911275 1104 88-89 5.58493317364575 1104 90-91 5.395228153038724 1104 92-93 5.2928618261120555 1104 94-95 4.9526097880935485 1104 96-97 4.759425428594858 1104 98-99 4.508395776486754 1104 100-101 4.177991136308423 1105 1 5.763882301582662 1105 2 6.753482003817567 1105 3 6.602178277680149 1105 4 8.41528894376474 1105 5 8.297094527924632 1105 6 7.390693283383332 1105 7 7.163934414499458 1105 8 6.34314408008192 1105 9 6.26759440503886 1105 10-11 5.727463350780273 1105 12-13 5.446474486727983 1105 14-15 5.82786888141386 1105 16-17 6.594598275147835 1105 18-19 7.312092741807195 1105 20-21 7.5365152786527965 1105 22-23 7.42709073022618 1105 24-25 7.478373098892707 1105 26-27 7.358891149459236 1105 28-29 7.163604562038678 1105 30-31 6.974237754415402 1105 32-33 6.773524822960987 1105 34-35 9.031010870731087 1105 36-37 -3.8776338334013154 1105 38-39 -2.416645882119809 1105 40-41 5.197489967392475 1105 42-43 7.273330318411148 1105 44-45 -3.4456346900122536 1105 46-47 -4.347117205484697 1105 48-49 4.499192041940763 1105 50-51 -9.759434788016755 1105 52-53 0.4029704373687686 1105 54-55 4.822043700956026 1105 56-57 5.800701031284639 1105 58-59 6.298437389432163 1105 60-61 6.115382518550611 1105 62-63 6.289982005783845 1105 64-65 6.214431650103993 1105 66-67 6.13849005416688 1105 68-69 5.939693799809572 1105 70-71 6.075104841661972 1105 72-73 5.835708529390516 1105 74-75 5.805997381207099 1105 76-77 5.652474522427678 1105 78-79 5.443077911881716 1105 80-81 5.317582847700242 1105 82-83 5.328074230835158 1105 84-85 5.202923899121757 1105 86-87 5.0758430056002215 1105 88-89 5.124338480914082 1105 90-91 5.0267852061399925 1105 92-93 4.605970481053191 1105 94-95 4.257186886546652 1105 96-97 4.112273779156171 1105 98-99 3.7542534826092524 1105 100-101 3.5919349145245167 1106 1 5.793361732997255 1106 2 7.092851824416783 1106 3 6.634288617026623 1106 4 7.978831177871584 1106 5 7.973974345129552 1106 6 7.248255270617481 1106 7 6.98634494177384 1106 8 6.126989234108294 1106 9 6.141829405990922 1106 10-11 5.654572241343306 1106 12-13 5.542406758172046 1106 14-15 5.981090644624697 1106 16-17 6.411809615396688 1106 18-19 6.763862244501933 1106 20-21 7.320869907722841 1106 22-23 6.988798266383679 1106 24-25 6.981158447138853 1106 26-27 7.085580430945438 1106 28-29 6.824176293376279 1106 30-31 6.526813111850974 1106 32-33 6.372290211110762 1106 34-35 8.903859841266971 1106 36-37 -0.20077682418705223 1106 38-39 1.0589397161449625 1106 40-41 6.33003432086252 1106 42-43 7.590476256436332 1106 44-45 -3.643221720502936 1106 46-47 -4.511635422901559 1106 48-49 4.284549974237041 1106 50-51 -9.746089381488122 1106 52-53 0.1301529675241646 1106 54-55 4.583467937861091 1106 56-57 5.519440838058323 1106 58-59 5.878505730616524 1106 60-61 5.797465165436552 1106 62-63 5.771396515142985 1106 64-65 5.843163799022371 1106 66-67 5.8057831885891495 1106 68-69 5.618596863779569 1106 70-71 5.63344271451674 1106 72-73 5.512112524721292 1106 74-75 5.484113714377617 1106 76-77 5.321896043898651 1106 78-79 5.143234007948479 1106 80-81 5.054390763852934 1106 82-83 4.963739677764151 1106 84-85 4.84214735120014 1106 86-87 4.467241826704443 1106 88-89 4.5197666171162805 1106 90-91 4.3823052366058395 1106 92-93 4.212270950459025 1106 94-95 4.01053846075334 1106 96-97 3.8147288461727538 1106 98-99 3.5327805251869275 1106 100-101 3.288099901982191 1107 1 5.783893403224212 1107 2 7.0158810188087095 1107 3 6.4536182181921085 1107 4 7.995388334876353 1107 5 8.325452152644633 1107 6 6.803345096367803 1107 7 6.975918688919467 1107 8 6.213600163794581 1107 9 6.15506165977833 1107 10-11 5.749609031024196 1107 12-13 5.42341256103828 1107 14-15 5.761507935365476 1107 16-17 6.666021399552985 1107 18-19 7.00081262958636 1107 20-21 7.460777667521828 1107 22-23 7.31493273082577 1107 24-25 7.341239024315339 1107 26-27 7.269070294344818 1107 28-29 7.321462737258717 1107 30-31 7.045348481507464 1107 32-33 6.7796713096449075 1107 34-35 9.199239851252159 1107 36-37 -0.20247009073422362 1107 38-39 1.006022445865625 1107 40-41 6.904354264225237 1107 42-43 7.890427762808024 1107 44-45 -3.301124667769697 1107 46-47 -4.19807504935739 1107 48-49 4.792066732161761 1107 50-51 -9.641406206526383 1107 52-53 0.46671569004239544 1107 54-55 5.218668009885384 1107 56-57 6.366021525726616 1107 58-59 6.817777712348544 1107 60-61 6.787598696836692 1107 62-63 6.576495998696075 1107 64-65 6.497489471590118 1107 66-67 6.405485024390131 1107 68-69 6.396455491553116 1107 70-71 6.436747524593592 1107 72-73 6.428383344243148 1107 74-75 6.4592481460814 1107 76-77 6.430605499542327 1107 78-79 6.296975137911403 1107 80-81 6.139311987283746 1107 82-83 5.888370839728777 1107 84-85 5.883367630949035 1107 86-87 5.425974935780323 1107 88-89 5.245637988278736 1107 90-91 5.177870463605126 1107 92-93 4.957288670071733 1107 94-95 4.68512522137897 1107 96-97 4.593856738398221 1107 98-99 4.402786213469065 1107 100-101 3.8257422931886804 1108 1 6.067568996241665 1108 2 6.54913821420481 1108 3 6.768255395648861 1108 4 8.066533179880693 1108 5 8.221535100474501 1108 6 7.460954076311996 1108 7 7.6094960006164705 1108 8 6.606762752388171 1108 9 4.862602203831631 1108 10-11 5.907848616012263 1108 12-13 5.331055478124146 1108 14-15 6.148673317555467 1108 16-17 6.232158470304309 1108 18-19 6.796850849386711 1108 20-21 7.636619316658315 1108 22-23 7.679862385469232 1108 24-25 7.3950566318351 1108 26-27 7.644808647807768 1108 28-29 7.546513763315698 1108 30-31 7.3895392130574535 1108 32-33 7.328681115312651 1108 34-35 9.83894827263753 1108 36-37 -3.4646266075479524 1108 38-39 -2.0744841963470435 1108 40-41 5.780191701305132 1108 42-43 8.201068741545562 1108 44-45 -2.951719249701016 1108 46-47 -3.97959800065318 1108 48-49 5.389404110674011 1108 50-51 -9.705103293066898 1108 52-53 1.001929205208203 1108 54-55 5.760690695621033 1108 56-57 6.7577286281365545 1108 58-59 7.2701196158130195 1108 60-61 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-10.093233381595208 2313 92-93 -9.514546004245943 2313 94-95 -9.139533481692704 2313 96-97 -8.345297138207702 2313 98-99 -7.435861450121715 2313 100-101 -6.346972154068059 2314 1 -20.677141864125048 2314 2 -19.556644578460915 2314 3 -19.9812509513187 2314 4 -21.8939745775241 2314 5 -21.860270259186994 2314 6 -22.6372123552818 2314 7 -22.712647863981555 2314 8 -23.518766161575158 2314 9 -24.6990339033616 2314 10-11 -24.975931355778993 2314 12-13 -24.9181687813963 2314 14-15 -25.117758276238614 2314 16-17 -24.214809089639278 2314 18-19 -23.586929122404545 2314 20-21 -23.095820739759173 2314 22-23 -23.00659741706956 2314 24-25 -22.70071205645827 2314 26-27 -22.386362015097728 2314 28-29 -22.323161836120125 2314 30-31 -22.225129334192193 2314 32-33 -21.93619899752233 2314 34-35 -19.11916174146684 2314 36-37 -17.45574085296403 2314 38-39 -16.57659985220036 2314 40-41 -17.894138341007984 2314 42-43 -17.80880431910324 2314 44-45 -15.830703734891426 2314 46-47 -16.646311681894364 2314 48-49 -16.119622687633463 2314 50-51 -14.134722475013302 2314 52-53 -17.05632185261972 2314 54-55 -18.627179544153297 2314 56-57 -18.82012750726542 2314 58-59 -18.43904822621801 2314 60-61 -18.121815410103014 2314 62-63 -17.625438017231115 2314 64-65 -17.1297858412654 2314 66-67 -16.705152063209415 2314 68-69 -16.24845349593266 2314 70-71 -15.649003328648728 2314 72-73 -15.16817524506288 2314 74-75 -14.648260386341807 2314 76-77 -14.087312589194877 2314 78-79 -13.599355920109076 2314 80-81 -13.089925782909654 2314 82-83 -12.609353847415706 2314 84-85 -12.098572073260293 2314 86-87 -11.535060331568937 2314 88-89 -10.98843482173264 2314 90-91 -10.405320662674738 2314 92-93 -9.788160704217233 2314 94-95 -9.139533481692704 2314 96-97 -8.419084103467535 2314 98-99 -7.627219474813073 2314 100-101 -6.581396500895506 2315 1 -20.677141864125048 2315 2 -19.556644578460915 2315 3 -19.9812509513187 2315 4 -16.640766707549766 2315 5 -21.860270259186994 2315 6 -22.6372123552818 2315 7 -22.712647863981555 2315 8 -23.518766161575158 2315 9 -19.819649814396673 2315 10-11 -17.98662425569345 2315 12-13 -14.564875368222648 2315 14-15 -14.509118413107734 2315 16-17 -19.989545331441388 2315 18-19 -23.586929122404545 2315 20-21 -23.095820739759173 2315 22-23 -23.00659741706956 2315 24-25 -22.70071205645827 2315 26-27 -22.386362015097728 2315 28-29 -22.323161836120125 2315 30-31 -22.225129334192193 2315 32-33 -21.93619899752233 2315 34-35 -19.11916174146684 2315 36-37 -17.45574085296403 2315 38-39 -16.57659985220036 2315 40-41 -17.894138341007984 2315 42-43 -17.80880431910324 2315 44-45 -15.830703734891426 2315 46-47 -16.646311681894364 2315 48-49 -16.119622687633463 2315 50-51 -14.134722475013302 2315 52-53 -17.05632185261972 2315 54-55 -18.627179544153297 2315 56-57 -18.82012750726542 2315 58-59 -18.43904822621801 2315 60-61 -18.121815410103014 2315 62-63 -17.625438017231115 2315 64-65 -17.1297858412654 2315 66-67 -16.705152063209415 2315 68-69 -16.24845349593266 2315 70-71 -15.649003328648728 2315 72-73 -15.16817524506288 2315 74-75 -14.648260386341807 2315 76-77 -14.087312589194877 2315 78-79 -13.599355920109076 2315 80-81 -13.089925782909654 2315 82-83 -12.609353847415706 2315 84-85 -12.098572073260293 2315 86-87 -11.535060331568937 2315 88-89 -10.98843482173264 2315 90-91 -10.405320662674738 2315 92-93 -9.788160704217233 2315 94-95 -9.139533481692704 2315 96-97 -8.419084103467535 2315 98-99 -7.627219474813073 2315 100-101 -6.581396500895506 2316 1 -20.677141864125048 2316 2 -19.556644578460915 2316 3 -19.9812509513187 2316 4 -21.8939745775241 2316 5 -21.860270259186994 2316 6 -22.6372123552818 2316 7 -22.712647863981555 2316 8 -23.518766161575158 2316 9 -24.6990339033616 2316 10-11 -24.975931355778993 2316 12-13 -24.9181687813963 2316 14-15 -25.117758276238614 2316 16-17 -24.214809089639278 2316 18-19 -23.586929122404545 2316 20-21 -23.095820739759173 2316 22-23 -23.00659741706956 2316 24-25 -22.70071205645827 2316 26-27 -22.386362015097728 2316 28-29 -22.323161836120125 2316 30-31 -22.225129334192193 2316 32-33 -21.93619899752233 2316 34-35 -19.11916174146684 2316 36-37 -17.45574085296403 2316 38-39 -16.57659985220036 2316 40-41 -17.894138341007984 2316 42-43 -17.80880431910324 2316 44-45 -15.830703734891426 2316 46-47 -16.646311681894364 2316 48-49 -16.119622687633463 2316 50-51 -14.134722475013302 2316 52-53 -17.05632185261972 2316 54-55 -18.627179544153297 2316 56-57 -18.82012750726542 2316 58-59 -18.43904822621801 2316 60-61 -18.121815410103014 2316 62-63 -17.625438017231115 2316 64-65 -17.1297858412654 2316 66-67 -16.705152063209415 2316 68-69 -16.24845349593266 2316 70-71 -15.649003328648728 2316 72-73 -15.16817524506288 2316 74-75 -14.648260386341807 2316 76-77 -14.087312589194877 2316 78-79 -13.599355920109076 2316 80-81 -13.089925782909654 2316 82-83 -12.609353847415706 2316 84-85 -12.098572073260293 2316 86-87 -11.535060331568937 2316 88-89 -10.98843482173264 2316 90-91 -10.405320662674738 2316 92-93 -9.788160704217233 2316 94-95 -9.139533481692704 2316 96-97 -8.419084103467535 2316 98-99 -7.627219474813073 2316 100-101 -6.581396500895506 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 540724.0 3 33764.0 4 73288.0 5 42303.0 6 36869.0 7 45246.0 8 57566.0 9 68603.0 10 71357.0 11 66655.0 12 59986.0 13 55049.0 14 53428.0 15 56873.0 16 62027.0 17 65984.0 18 68314.0 19 71502.0 20 74455.0 21 78916.0 22 83675.0 23 87956.0 24 93234.0 25 98573.0 26 104514.0 27 110546.0 28 116961.0 29 123913.0 30 128215.0 31 128505.0 32 123142.0 33 119566.0 34 111043.0 35 91951.0 36 63669.0 37 26521.0 38 3245.0 39 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.82408026114966 20.47532985261538 14.153330676603733 23.547259209631225 2 15.298814949158245 20.960879207568546 40.673868362487504 23.066437480785705 3 18.373700315679535 25.954299813408753 30.5644751974464 25.107524673465313 4 12.523004530785919 17.11850477013489 39.22012975769736 31.138360941381833 5 13.178643442329701 37.65056908045092 35.7898017123439 13.380985764875483 6 30.384000683917222 37.86499734837178 18.214621049001508 13.536380918709485 7 26.486462742050517 32.65305685144783 23.326882343298617 17.533598063203037 8 24.99843820377903 36.00856658170265 21.194454846831796 17.79854036768652 9 25.984294076237934 16.18693370468973 21.2751438792337 36.55362833983864 10-11 24.533486014964012 26.407134935041082 29.300352327248348 19.759026722746555 12-13 24.904341558266346 24.217695247158392 29.21814653297704 21.65981666159822 14-15 22.274613130935762 25.631869752443126 26.7554777775593 25.33803933906181 16-17 22.05579569073622 28.6377707651103 27.34558494317836 21.960848600975126 18-19 21.9773598695147 27.75924701864307 29.147546175774448 21.115846936067783 20-21 22.588106617906423 26.999192905570307 29.14457456264823 21.268125913875043 22-23 22.458518646867507 26.827757686771115 28.854106055181106 21.859617611180276 24-25 22.257417974847648 27.04790994884502 28.77596432640257 21.918707749904762 26-27 22.235299412416083 27.16595250904123 28.700641311454362 21.898106767088326 28-29 22.174251256635575 27.34550312612795 28.700173110676563 21.780072506559904 30-31 22.43137187175024 27.152808752340974 28.55459877320464 21.86122060270415 32-33 22.342432834030237 27.271235270073962 28.406694930673893 21.979636965221903 34-35 22.649406690673718 27.19272882084198 28.438810860024695 21.71905362845961 36-37 23.10087607092366 27.171958700626277 28.063407348115522 21.663757880334547 38-39 22.90202215753467 27.211518494554486 28.095942491930874 21.79051685597997 40-41 22.845251339974805 27.072210922725727 28.282226577169954 21.800311160129514 42-43 22.96755278016977 27.06147536350963 27.93776766255529 22.033204193765314 44-45 23.211687921055297 27.306858091984637 27.600229922935366 21.881224064024693 46-47 22.631769205024096 27.21560284255747 27.985312768517378 22.167315183901064 48-49 23.08764639309201 27.307728835708396 27.49399939921457 22.110625371985023 50-51 23.25286669387035 27.442792315306846 27.31816015261805 21.986180838204756 52-53 22.412220986808506 27.489372411921774 27.818249308007786 22.280157293261933 54-55 22.19574828904054 27.427832791652158 27.979610794281285 22.39680812502602 56-57 22.291692867568774 27.58181524007302 27.768105819423905 22.3583860729343 58-59 22.358775239723368 27.476677020750873 27.876702329046754 22.287845410479 60-61 22.34241068491119 27.5540691417974 27.857074969354755 22.246445203936656 62-63 22.24278289822709 27.529724386603117 27.812400332195566 22.41509238297423 64-65 22.13677651595167 27.38504250096327 27.85715405720483 22.62102692588023 66-67 22.21848492119967 27.51034768819715 27.703213151752333 22.567954238850852 68-69 22.191509481470707 27.485126624775486 27.72663974328128 22.596724150472536 70-71 22.172091075133853 27.30848110253266 27.656825558636577 22.862602263696907 72-73 22.281488193128713 27.39690223694305 27.573965588724114 22.747643981204124 74-75 22.25836746633959 27.575303051000926 27.453417572059724 22.71291191059976 76-77 22.29986595619228 27.40632931795075 27.50611786615399 22.787686859702983 78-79 22.178051052627147 27.498578738896583 27.46803043553079 22.85533977294548 80-81 22.322153357395162 27.521508898088687 27.366013157252606 22.790324587263544 82-83 22.443872619050236 27.411600568340898 27.44456623285249 22.69996057975638 84-85 22.3730139279076 27.50787069576073 27.284223432561827 22.834891943769843 86-87 22.24023064912161 27.509174774121693 27.315907743056282 22.934686833700415 88-89 22.341894836368535 27.37086454941308 27.191590400140726 23.095650214077658 90-91 22.388166871307426 27.366005387287025 27.179348966852206 23.066478774553342 92-93 22.393829582441196 27.236592742021582 27.263700627820004 23.105877047717225 94-95 22.26783738685021 27.216251395747076 27.278930679448564 23.236980537954143 96-97 22.294431628706956 27.057921291349896 27.16588657706484 23.48176050287831 98-99 22.206822629304487 27.11850759655369 27.087183703421847 23.587486070719972 100-101 22.564836891638635 27.163332845816594 26.949630450975658 23.32219981156911 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 39112.0 1 42570.5 2 31884.0 3 18847.0 4 24571.0 5 31210.0 6 32948.0 7 31323.5 8 28726.0 9 25972.5 10 23161.0 11 20283.5 12 17252.5 13 14540.5 14 12406.5 15 10674.0 16 9357.5 17 8595.0 18 8293.5 19 8526.0 20 9112.0 21 9910.5 22 11260.5 23 13193.0 24 15659.5 25 19009.5 26 22965.5 27 27846.0 28 34241.5 29 41826.0 30 50161.0 31 58432.5 32 66694.0 33 73932.0 34 78900.0 35 82966.0 36 87658.0 37 91812.0 38 94349.5 39 95668.0 40 96355.5 41 96931.0 42 97509.0 43 98982.0 44 100144.0 45 101082.5 46 102199.5 47 102175.5 48 101204.0 49 99056.5 50 95840.0 51 92299.5 52 88660.5 53 84425.5 54 79129.5 55 72866.0 56 65317.0 57 57571.0 58 50297.0 59 43166.5 60 36349.5 61 29885.0 62 23991.0 63 18844.5 64 14579.5 65 11087.5 66 8296.5 67 6179.5 68 4648.0 69 3536.5 70 2659.5 71 1947.5 72 1424.0 73 1024.5 74 744.5 75 507.5 76 332.0 77 223.0 78 146.5 79 87.5 80 51.0 81 30.5 82 20.0 83 17.0 84 13.5 85 5.5 86 3.5 87 5.5 88 5.0 89 2.5 90 1.0 91 0.5 92 0.0 93 0.5 94 1.5 95 1.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.558416038513583 2 21.593730566821932 3 17.323138561155744 4 22.60056437511692 5 22.455362245809106 6 16.299780392770398 7 15.101567206694883 8 12.153924565841388 9 3.047577119491741 10-11 6.5602315957286494 12-13 3.5886058927990474 14-15 7.481169051480507 16-17 11.592111482732346 18-19 14.704270046246984 20-21 15.117894487261236 22-23 13.032232204552779 24-25 15.595524293689724 26-27 13.093569416411146 28-29 13.100497519678392 30-31 13.975409326591395 32-33 12.432246333638655 34-35 19.309533403614925 36-37 24.090379456915535 38-39 23.12578130661299 40-41 18.25627978743306 42-43 18.189454668829082 44-45 23.541801021842172 46-47 12.895838013972028 48-49 21.624520452458203 50-51 21.312604205798323 52-53 11.334028266054933 54-55 12.965482885765734 56-57 12.48604904369435 58-59 13.502721668226627 60-61 12.909436501128962 62-63 12.872400710577804 64-65 13.952214582611342 66-67 12.24668838179822 68-69 13.301018355380467 70-71 11.902390453346577 72-73 11.256045414399116 74-75 11.330389874842375 76-77 11.399746707331753 78-79 11.814235266258985 80-81 11.777320755414912 82-83 10.279107053900644 84-85 10.18467564197139 86-87 10.326815458675304 88-89 11.75003282132073 90-91 9.21390738657088 92-93 11.247737754463776 94-95 11.356252772378305 96-97 10.906577938432962 98-99 10.75511474424687 100-101 10.857292897466193 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3298161.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.34764277515676 #Duplication Level Percentage of deduplicated Percentage of total 1 98.96932736323184 96.3443072585344 2 0.7192808779198145 1.4004059591747848 3 0.14162402251420902 0.4136029425628194 4 0.05205556438213981 0.2026994594372688 5 0.03360741766779702 0.1635801444860098 6 0.019810346215738635 0.1157094303997142 7 0.012289198967560894 0.083742718576086 8 0.008930134992530071 0.06954620729493956 9 0.006138298815233722 0.05377940282812863 >10 0.03392985638102458 0.5668180126201565 >50 0.0018685214009756227 0.12212149361987988 >100 0.0010137628812119297 0.16051644566853593 >500 3.1158657492257594E-5 0.02608570798662388 >1k 6.231731498435513E-5 0.09845838153995776 >5k 3.115865749217757E-5 0.17862643527073666 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5889 0.17855404875626144 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.0319926771312862E-5 2 0.0 0.0 0.0 0.0 3.0319926771312862E-5 3 0.0 0.0 0.0 0.0 3.0319926771312862E-5 4 0.0 0.0 0.0 0.0 3.0319926771312862E-5 5 0.0 3.0319926771312862E-5 0.0 0.0 3.0319926771312862E-5 6 3.0319926771312862E-5 3.0319926771312862E-5 0.0 0.0 1.2127970708525145E-4 7 3.0319926771312862E-5 3.0319926771312862E-5 0.0 0.0 1.2127970708525145E-4 8 3.0319926771312862E-5 3.0319926771312862E-5 0.0 0.0 1.2127970708525145E-4 9 3.0319926771312862E-5 3.0319926771312862E-5 0.0 0.0 1.2127970708525145E-4 10-11 3.0319926771312862E-5 3.0319926771312862E-5 0.0 0.0 1.2127970708525145E-4 12-13 6.0639853542625725E-5 7.579981692828215E-5 0.0 0.0 2.577193775561593E-4 14-15 6.0639853542625725E-5 9.095978031393858E-5 0.0 0.0 3.486791578700979E-4 16-17 6.0639853542625725E-5 9.095978031393858E-5 0.0 0.0 4.0931901141272363E-4 18-19 6.0639853542625725E-5 9.095978031393858E-5 0.0 0.0 4.244789747983801E-4 20-21 6.0639853542625725E-5 9.095978031393858E-5 0.0 1.5159963385656431E-5 4.3963893818403654E-4 22-23 6.0639853542625725E-5 9.095978031393858E-5 0.0 9.095978031393858E-5 4.851188283410058E-4 24-25 6.0639853542625725E-5 9.095978031393858E-5 0.0 2.2739945078484647E-4 5.154387551123186E-4 26-27 6.0639853542625725E-5 9.095978031393858E-5 0.0 4.0931901141272363E-4 5.60918645269288E-4 28-29 6.0639853542625725E-5 9.095978031393858E-5 0.0 0.0010308775102246372 5.760786086549444E-4 30-31 6.0639853542625725E-5 9.095978031393858E-5 0.0 0.0032139122377591632 5.760786086549444E-4 32-33 6.0639853542625725E-5 1.0611974369959502E-4 0.0 0.009535616969577894 5.760786086549444E-4 34-35 6.0639853542625725E-5 1.2127970708525145E-4 0.0 0.019313793353326295 5.760786086549444E-4 36-37 6.0639853542625725E-5 1.3643967047090788E-4 0.0 0.033533839009072024 5.760786086549444E-4 38-39 6.0639853542625725E-5 1.515996338565643E-4 0.0 0.0529234321793266 5.760786086549444E-4 40-41 6.0639853542625725E-5 1.515996338565643E-4 0.0 0.07525405824639853 5.912385720406009E-4 42-43 6.0639853542625725E-5 1.515996338565643E-4 0.0 0.09920680039573568 6.063985354262572E-4 44-45 7.579981692828215E-5 1.6675959724222073E-4 0.0 0.12737401236628534 6.518784255832265E-4 46-47 9.095978031393858E-5 1.8191956062787716E-4 0.0 0.16198420877573896 7.276782425115086E-4 48-49 9.095978031393858E-5 1.8191956062787716E-4 0.0 0.20020247647097883 7.579981692828216E-4 50-51 9.095978031393858E-5 1.8191956062787716E-4 0.0 0.24587944615196164 7.579981692828216E-4 52-53 9.095978031393858E-5 1.8191956062787716E-4 0.0 0.30480622383200817 8.79277876368073E-4 54-55 9.095978031393858E-5 1.8191956062787716E-4 0.0 0.37082786437654197 8.79277876368073E-4 56-57 9.095978031393858E-5 1.8191956062787716E-4 0.0 0.4476282388882774 8.944378397537294E-4 58-59 9.095978031393858E-5 1.8191956062787716E-4 0.0 0.5327969131888952 9.095978031393859E-4 60-61 9.095978031393858E-5 1.8191956062787716E-4 0.0 0.6193754640843792 9.399177299106987E-4 62-63 9.095978031393858E-5 1.8191956062787716E-4 0.0 0.7101230049109186 0.0010308775102246372 64-65 9.095978031393858E-5 1.8191956062787716E-4 0.0 0.8081473281625731 0.0010308775102246372 66-67 9.095978031393858E-5 1.8191956062787716E-4 0.0 0.9184209018298379 0.0010611974369959501 68-69 9.095978031393858E-5 1.8191956062787716E-4 3.0319926771312862E-5 1.0417775238989244 0.0010611974369959501 70-71 9.095978031393858E-5 1.8191956062787716E-4 3.0319926771312862E-5 1.1716529302238428 0.0010611974369959501 72-73 9.095978031393858E-5 1.970795240135336E-4 3.0319926771312862E-5 1.310563674726613 0.0011066773271529195 74-75 9.095978031393858E-5 2.1223948739919004E-4 3.0319926771312862E-5 1.452733811357299 0.001121837290538576 76-77 9.095978031393858E-5 2.2739945078484647E-4 3.0319926771312862E-5 1.6098971517763991 0.001121837290538576 78-79 9.095978031393858E-5 2.425594141705029E-4 3.0319926771312862E-5 1.7865107252192964 0.0011369972539242324 80-81 9.095978031393858E-5 2.425594141705029E-4 3.0319926771312862E-5 1.9766015061120426 0.0011521572173098888 82-83 9.095978031393858E-5 2.425594141705029E-4 3.0319926771312862E-5 2.1724227531645663 0.0012127970708525144 84-85 9.095978031393858E-5 2.7287934094181576E-4 3.0319926771312862E-5 2.373428707694985 0.0012127970708525144 86-87 9.095978031393858E-5 2.7287934094181576E-4 3.0319926771312862E-5 2.5944761338212414 0.0012582769610094838 88-89 9.095978031393858E-5 2.7287934094181576E-4 3.0319926771312862E-5 2.827181571791068 0.001303756851166453 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2390 0.0 52.31391 1 GTATCAA 4695 0.0 46.066486 1 TCAACGC 5840 0.0 37.341835 4 ATCAACG 5800 0.0 37.094234 3 TATCAAC 5985 0.0 36.293255 2 CAACGCA 5945 0.0 36.269676 5 AACGCAG 6395 0.0 33.68723 6 ACGCAGA 7510 0.0 28.096466 7 GTACATG 6275 0.0 26.754318 1 CGCAGAG 7990 0.0 26.44757 8 TACATGG 6535 0.0 25.603851 2 ACATGGG 6605 0.0 24.868599 3 GCAGAGT 8510 0.0 23.361477 9 CATGGGG 4850 0.0 22.430172 4 AGAGTAC 7610 0.0 19.809101 10-11 GAGTACT 5340 0.0 19.193222 12-13 GAGTACA 4525 0.0 19.052015 1 CAGAGTA 8235 0.0 18.735138 10-11 GTGGTAT 1535 0.0 18.064156 1 TGGTATC 1455 0.0 17.665258 2 >>END_MODULE