FastQCFastQC Report
Sat 18 Jun 2016
SRR1930946_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930946_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3298161
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51790.1570269007486293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43620.1322555205764667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35750.052.4646451
GTATCAA63900.039.458391
CAACGCA79250.031.4385155
ATCAACG79450.031.3698353
TCAACGC79750.031.1913594
AACGCAG84650.029.712856
TATCAAC85100.029.5669362
ACGCAGA99300.024.9946987
GTACATG83450.024.5242981
CGCAGAG101950.024.1122638
TACATGG88050.022.6993162
ACATGGG85900.022.6035693
GTGGTAT19750.021.8786221
GCAGAGT117650.020.6928759
CATGGGG60750.019.6919164
TGGTATC19650.019.5696282
AGAGTAC107650.018.80184410-11
CAGAGTA112600.018.1438810-11
GAGTACA64700.018.0541271
GAGTACT75500.017.56832112-13
Produced by FastQC (version 0.11.5)