##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930946_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3298161 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.24412725758385 31.0 31.0 34.0 30.0 34.0 2 31.373717959796384 31.0 31.0 34.0 30.0 34.0 3 31.455576607691377 31.0 31.0 34.0 30.0 34.0 4 35.32362064799141 37.0 35.0 37.0 33.0 37.0 5 35.106709163076026 37.0 35.0 37.0 32.0 37.0 6 35.14322072209331 37.0 35.0 37.0 32.0 37.0 7 35.02291428465742 36.0 35.0 37.0 32.0 37.0 8 35.001823440396024 36.0 35.0 37.0 32.0 37.0 9 36.50654106940201 38.0 35.0 39.0 32.0 39.0 10-11 36.436854962507894 38.0 35.0 39.0 32.0 39.0 12-13 36.33619204762897 38.0 35.0 39.0 32.0 39.0 14-15 37.35748527740156 39.0 36.0 41.0 32.0 41.0 16-17 37.32268724904576 39.0 36.0 41.0 32.0 41.0 18-19 37.31021802756142 39.0 36.0 40.5 32.0 41.0 20-21 37.27620088891962 39.0 36.0 40.0 31.0 41.0 22-23 37.14443715755537 39.0 36.0 40.0 31.0 41.0 24-25 37.026538577104034 39.0 36.0 40.0 31.0 41.0 26-27 36.75232561418318 39.0 35.5 40.0 30.0 41.0 28-29 36.57229149820157 38.0 35.0 40.0 30.0 41.0 30-31 36.33973326347622 38.0 35.0 40.0 30.0 41.0 32-33 36.111162402320566 38.0 35.0 40.0 30.0 41.0 34-35 36.313290042541894 38.0 35.0 40.0 30.0 41.0 36-37 36.36115520133796 38.0 35.0 40.0 30.0 41.0 38-39 36.33266235335388 38.0 35.0 40.0 30.0 41.0 40-41 36.239926431729685 38.0 35.0 40.0 30.0 41.0 42-43 36.105645085245996 38.0 35.0 40.0 29.5 41.0 44-45 35.92569844225312 38.0 35.0 40.0 29.0 41.0 46-47 35.71770374460192 38.0 34.0 40.0 28.0 41.0 48-49 35.52089679673006 38.0 34.0 40.0 27.5 41.0 50-51 35.25037361729764 38.0 34.0 40.0 27.0 41.0 52-53 35.04454755240875 38.0 33.5 40.0 26.5 41.0 54-55 34.87543133885823 38.0 33.0 40.0 26.0 41.0 56-57 34.62007706718987 37.5 33.0 40.0 26.0 41.0 58-59 34.37376677487849 37.0 33.0 40.0 26.0 41.0 60-61 34.066759779161785 37.0 32.5 40.0 25.0 41.0 62-63 33.763088884987724 36.5 32.0 39.0 24.5 41.0 64-65 33.455823411895295 36.0 32.0 39.0 23.5 41.0 66-67 33.11862171070484 35.5 31.5 39.0 23.0 40.0 68-69 32.744485335919016 35.0 31.0 38.5 22.5 40.0 70-71 32.34809944087023 35.0 31.0 38.0 22.0 40.0 72-73 31.925733158569276 35.0 30.5 37.5 21.5 39.5 74-75 31.406126626322973 34.0 30.0 37.0 20.0 39.0 76-77 30.027590830162627 33.0 28.5 35.5 18.5 38.0 78-79 30.797783370793603 34.0 30.0 36.0 20.0 38.5 80-81 30.795073527338417 34.0 30.0 36.0 20.0 37.5 82-83 30.51727659747356 34.0 30.0 35.0 19.0 37.0 84-85 30.189528194651505 34.0 30.0 35.0 18.0 37.0 86-87 29.846207022640797 34.0 29.0 35.0 16.5 36.0 88-89 29.493319155735577 34.0 29.0 35.0 11.5 36.0 90-91 29.150913948712628 34.0 29.0 35.0 7.0 35.5 92-93 28.790756727764354 34.0 29.0 35.0 2.0 35.0 94-95 28.371371955462454 33.0 29.0 35.0 2.0 35.0 96-97 27.90473251609003 33.0 27.5 35.0 2.0 35.0 98-99 27.407251647205822 33.0 27.0 35.0 2.0 35.0 100-101 26.17707549752726 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5207668847424181 1101 2 0.6547066996218227 1101 3 0.6133126979792678 1101 4 0.42841409695466837 1101 5 0.5030554079275973 1101 6 0.42590671026466964 1101 7 0.5549476546070835 1101 8 0.48279195503966577 1101 9 0.7279517010964653 1101 10-11 0.6186998487271254 1101 12-13 0.6939735308852732 1101 14-15 0.7641585875892574 1101 16-17 0.70508931684018 1101 18-19 0.7353631692847316 1101 20-21 0.8251642135445891 1101 22-23 0.8485883047880094 1101 24-25 0.6720423153390911 1101 26-27 0.8026924995922471 1101 28-29 0.7754543376769476 1101 30-31 0.7484250928927025 1101 32-33 0.8006232339978467 1101 34-35 0.7873703802601071 1101 36-37 0.8301717391652872 1101 38-39 0.8429228104512063 1101 40-41 0.837562718199031 1101 42-43 0.8702153579554661 1101 44-45 0.9321014025737853 1101 46-47 0.8417548968474478 1101 48-49 0.888819664837051 1101 50-51 0.8396429458120949 1101 52-53 0.7720277584889388 1101 54-55 0.7864083732441216 1101 56-57 0.9768077524101173 1101 58-59 0.8373892323685439 1101 60-61 0.8285635437734626 1101 62-63 0.8398173636127382 1101 64-65 0.8049896641819885 1101 66-67 0.8655078028126368 1101 68-69 0.9201417987788858 1101 70-71 0.8551465034350514 1101 72-73 0.6968987777912155 1101 74-75 0.8832846569317816 1101 76-77 0.9665510734360794 1101 78-79 1.0115525989882315 1101 80-81 0.8861348633482642 1101 82-83 1.0281160772644071 1101 84-85 1.0605340428216294 1101 86-87 0.9400200979756015 1101 88-89 0.8955369033042047 1101 90-91 0.9281883321123452 1101 92-93 1.0754011963203922 1101 94-95 1.1382638823022155 1101 96-97 1.1380755781394676 1101 98-99 1.1527813207723163 1101 100-101 1.3937902319604518 1102 1 0.42787068962957875 1102 2 0.5039249186716823 1102 3 0.4406515559424484 1102 4 0.2819253113628335 1102 5 0.4465970531323791 1102 6 0.44644592308087994 1102 7 0.5447889860915467 1102 8 0.5107052257870421 1102 9 0.7054609077835323 1102 10-11 0.6722753932167862 1102 12-13 0.7185441665005712 1102 14-15 0.7903950517767484 1102 16-17 0.75562442194353 1102 18-19 0.7415901433029433 1102 20-21 0.7524235575259794 1102 22-23 0.83640815795534 1102 24-25 0.7879888568931435 1102 26-27 0.8746356997618108 1102 28-29 0.952148709017159 1102 30-31 0.928325268244059 1102 32-33 0.9287259746223739 1102 34-35 0.8284796091069637 1102 36-37 0.8696172238679907 1102 38-39 0.9298706964150156 1102 40-41 0.9265360197329571 1102 42-43 0.887199740864709 1102 44-45 0.8837690747067768 1102 46-47 0.9356894613705933 1102 48-49 0.8009328598409482 1102 50-51 0.8725839033223721 1102 52-53 0.8583962660977491 1102 54-55 0.7027785261614667 1102 56-57 0.7972513224035396 1102 58-59 0.91274867114047 1102 60-61 0.8735955735579992 1102 62-63 0.7591252630947594 1102 64-65 0.771429909466363 1102 66-67 0.858738367976116 1102 68-69 0.8200480736298914 1102 70-71 0.8215862855131988 1102 72-73 0.748558732908382 1102 74-75 0.9252755525206489 1102 76-77 1.0715326433865222 1102 78-79 1.0959470605980677 1102 80-81 0.9397933217534558 1102 82-83 1.0493238356352848 1102 84-85 1.0398582304875958 1102 86-87 1.090750324072122 1102 88-89 1.1459544374017412 1102 90-91 1.0777562811314993 1102 92-93 1.1388871771058575 1102 94-95 1.2110257068456214 1102 96-97 1.3023832589881472 1102 98-99 1.3619275501624166 1102 100-101 1.669093381911054 1103 1 0.3416731827342083 1103 2 0.4105174932623612 1103 3 0.36897595875127465 1103 4 0.18996866557113634 1103 5 0.20317001902471077 1103 6 0.383498860010441 1103 7 0.33603788853711336 1103 8 0.3613551446433618 1103 9 0.4473620874104043 1103 10-11 0.42385217021693933 1103 12-13 0.42574523446668167 1103 14-15 0.47616846081565 1103 16-17 0.5488559108451554 1103 18-19 0.4974476443835272 1103 20-21 0.5159970609160993 1103 22-23 0.5815924173470464 1103 24-25 0.5850674203472437 1103 26-27 0.6230747208074519 1103 28-29 0.5953161543711687 1103 30-31 0.643666336215631 1103 32-33 0.7506285036846307 1103 34-35 0.6840282879851358 1103 36-37 0.83400947577789 1103 38-39 0.7243346605262317 1103 40-41 0.7002487271255546 1103 42-43 0.8291151979185685 1103 44-45 0.8550673034772345 1103 46-47 0.732760285148089 1103 48-49 0.7731582108147919 1103 50-51 0.813401236847163 1103 52-53 0.7032271318552432 1103 54-55 0.7965548154527298 1103 56-57 0.9403344508099636 1103 58-59 0.8909123117605091 1103 60-61 0.6349669568412608 1103 62-63 0.6682489395759177 1103 64-65 0.6463481306774312 1103 66-67 0.41836602032914527 1103 68-69 0.43446125453591833 1103 70-71 0.27103422348579187 1103 72-73 0.3127025776209642 1103 74-75 0.6080445556803475 1103 76-77 0.7590429724402377 1103 78-79 0.821536553067407 1103 80-81 0.7550266170045461 1103 82-83 0.7719772134896701 1103 84-85 0.7230125784876797 1103 86-87 0.879255146937787 1103 88-89 0.7872768804333958 1103 90-91 0.7170494720098617 1103 92-93 0.7910270019214636 1103 94-95 0.8864126064504525 1103 96-97 0.7547762804950722 1103 98-99 0.9214137030159293 1103 100-101 1.0688603812229545 1104 1 0.3939587449529611 1104 2 0.4608824477725477 1104 3 0.44799688352714284 1104 4 0.23528407473649793 1104 5 0.3689147766376877 1104 6 0.38510588848888005 1104 7 0.4162507441055041 1104 8 0.47993893962536305 1104 9 0.522403054879554 1104 10-11 0.40846691808935276 1104 12-13 0.45231938224527113 1104 14-15 0.6115790266135761 1104 16-17 0.5714950470948921 1104 18-19 0.6835908003302791 1104 20-21 0.5967149057261381 1104 22-23 0.5868135028517898 1104 24-25 0.5222354354740162 1104 26-27 0.74935378827454 1104 28-29 0.5796330280053326 1104 30-31 0.6264898907186307 1104 32-33 0.6199160731922291 1104 34-35 0.677245380083555 1104 36-37 0.5769229669559408 1104 38-39 0.5411416135512965 1104 40-41 0.5449878325032742 1104 42-43 0.5979041382723977 1104 44-45 0.7104120939925949 1104 46-47 0.7441782914679109 1104 48-49 0.7930783010395359 1104 50-51 0.7163436495052835 1104 52-53 0.710707436840984 1104 54-55 0.6561633377275271 1104 56-57 0.8517166480129461 1104 58-59 0.7272200924646839 1104 60-61 0.7360033301230615 1104 62-63 0.6689611305888761 1104 64-65 0.6456235318532038 1104 66-67 0.6571648470187412 1104 68-69 0.6481597731820798 1104 70-71 0.6578041387603974 1104 72-73 0.6515115735097332 1104 74-75 0.8610197839623872 1104 76-77 0.9595758488884591 1104 78-79 0.9834249108788384 1104 80-81 0.8962356219865839 1104 82-83 0.9835500484697697 1104 84-85 1.0782415772258993 1104 86-87 1.0212000305724729 1104 88-89 1.01757848222109 1104 90-91 0.841791966542182 1104 92-93 0.9467841222263864 1104 94-95 0.9823685450111377 1104 96-97 0.9509935813570785 1104 98-99 1.0956632777230162 1104 100-101 1.3355950912853771 1105 1 0.12990928206840735 1105 2 0.20699609726574408 1105 3 0.2424387906974772 1105 4 0.11077177981528052 1105 5 0.07042480928542716 1105 6 0.20808298853747687 1105 7 0.14924779444125136 1105 8 0.1681851225790041 1105 9 0.3637909593282487 1105 10-11 0.2861403629342263 1105 12-13 0.22940633451815273 1105 14-15 0.2645159103135697 1105 16-17 0.20101826448255622 1105 18-19 0.3128279926913393 1105 20-21 0.2810983381461156 1105 22-23 0.4235021041977234 1105 24-25 0.3703391942517058 1105 26-27 0.4058973960389878 1105 28-29 0.45205265347708234 1105 30-31 0.3936547993534987 1105 32-33 0.3347155498711416 1105 34-35 0.53377798560701 1105 36-37 0.5581599911256347 1105 38-39 0.4203327693450234 1105 40-41 0.47431181293457314 1105 42-43 0.36166915500178476 1105 44-45 0.4984456112727642 1105 46-47 0.38666695184701894 1105 48-49 0.24170539753151132 1105 50-51 0.4150716275726012 1105 52-53 0.37819129412695673 1105 54-55 0.31289632696660163 1105 56-57 0.38632210299716263 1105 58-59 0.20291261871881972 1105 60-61 0.21489922033731546 1105 62-63 0.1729167021436666 1105 64-65 0.15598292288082405 1105 66-67 0.15587665411855056 1105 68-69 0.20422549811009816 1105 70-71 0.09323527703030976 1105 72-73 0.1349815373917984 1105 74-75 0.3748107274228296 1105 76-77 0.43563193106689013 1105 78-79 0.3632300814667566 1105 80-81 0.3938004205325605 1105 82-83 0.4822804696328973 1105 84-85 0.519850423958367 1105 86-87 0.40471812510399374 1105 88-89 0.4141299943145853 1105 90-91 0.31939077231706037 1105 92-93 0.3234652879188076 1105 94-95 0.23947554396542614 1105 96-97 0.018636588884469063 1105 98-99 0.20930196811729473 1105 100-101 0.5265196218952042 1106 1 0.08598614209936883 1106 2 0.1709006100191779 1106 3 0.1508384231204225 1106 4 0.04039277806845831 1106 5 0.17781170069289942 1106 6 0.14660001381253807 1106 7 0.18958416056124605 1106 8 0.17369985463685822 1106 9 0.2247594089643954 1106 10-11 0.22030263495779678 1106 12-13 0.16898958161437605 1106 14-15 0.18282079229981463 1106 16-17 0.17526473152066302 1106 18-19 0.25458710553915154 1106 20-21 0.08729904846394021 1106 22-23 0.21431164298786598 1106 24-25 0.22124083427456043 1106 26-27 0.23860132265604506 1106 28-29 0.14126505340754392 1106 30-31 0.2718475041079955 1106 32-33 0.2549799735450833 1106 34-35 0.251428224449306 1106 36-37 0.3823009453357997 1106 38-39 0.3359389403345858 1106 40-41 0.32990647644427185 1106 42-43 0.41992908330770007 1106 44-45 0.33856540496599763 1106 46-47 0.29892192117419825 1106 48-49 0.38551532542349065 1106 50-51 0.3225948288044762 1106 52-53 0.20837980154886537 1106 54-55 0.27107495782486524 1106 56-57 0.3395532626034239 1106 58-59 0.4036454547744839 1106 60-61 0.18763431649514217 1106 62-63 0.2615252491380602 1106 64-65 0.23715734812773803 1106 66-67 0.34961683343654926 1106 68-69 0.3125482208295054 1106 70-71 0.26213635409731495 1106 72-73 0.26665115681708684 1106 74-75 0.47373684774636615 1106 76-77 0.515619471529341 1106 78-79 0.528453401084942 1106 80-81 0.4303971454541191 1106 82-83 0.5022783171546479 1106 84-85 0.47630777591339424 1106 86-87 0.552698810751103 1106 88-89 0.7011523718095987 1106 90-91 0.6254247483583235 1106 92-93 0.6308328070220526 1106 94-95 0.5639646615321325 1106 96-97 0.6445116126859141 1106 98-99 0.5906294676826569 1106 100-101 0.9304562976409159 1107 1 0.08506185763615548 1107 2 0.17123748727505372 1107 3 0.18113364087113837 1107 4 0.046687946711699624 1107 5 0.13021493851219645 1107 6 0.12592527715135304 1107 7 0.21530157979992026 1107 8 0.06393376989372968 1107 9 0.1929677228593576 1107 10-11 0.12258637792928795 1107 12-13 0.13154758981531955 1107 14-15 0.0859383801408029 1107 16-17 0.1429241466506781 1107 18-19 0.11856841196158996 1107 20-21 0.24244682103086745 1107 22-23 0.12400668558859707 1107 24-25 0.16688983043599137 1107 26-27 0.1808274871169644 1107 28-29 0.1758798462149329 1107 30-31 0.26715139751203765 1107 32-33 0.40304464808532003 1107 34-35 0.28679755355940273 1107 36-37 0.4163532120548439 1107 38-39 0.34986696515022686 1107 40-41 0.22412460364899545 1107 42-43 0.3468426377883489 1107 44-45 0.40525695275166385 1107 46-47 0.19811725432747096 1107 48-49 0.23684761113076291 1107 50-51 0.28886998615300996 1107 52-53 0.23279808262366686 1107 54-55 0.353331568918243 1107 56-57 0.17738263050841852 1107 58-59 0.2325303059379209 1107 60-61 0.12084962820481593 1107 62-63 0.05230008319572477 1107 64-65 0.0785525732445791 1107 66-67 0.1709833306824322 1107 68-69 0.29712696056809307 1107 70-71 0.2613951995806545 1107 72-73 0.3117229764944476 1107 74-75 0.385126220889493 1107 76-77 0.3471028854807727 1107 78-79 0.44797370293219885 1107 80-81 0.36949872425933705 1107 82-83 0.4118308662416581 1107 84-85 0.40734418910366443 1107 86-87 0.33892878922566183 1107 88-89 0.4231998259964165 1107 90-91 0.41745030879734557 1107 92-93 0.37093822439463864 1107 94-95 0.4125451282168058 1107 96-97 0.48887882199014143 1107 98-99 0.41734450520895194 1107 100-101 0.8427318151108985 1108 1 0.31909712017163727 1108 2 0.39678324946474675 1108 3 0.25413925213281274 1108 4 0.1505814113649251 1108 5 0.22171059568128015 1108 6 0.1920266457087152 1108 7 0.3953261621644799 1108 8 0.32494256145870537 1108 9 0.2984284737125975 1108 10-11 0.4104679300841738 1108 12-13 0.3278342407248829 1108 14-15 0.45998753845154994 1108 16-17 0.522895095494647 1108 18-19 0.507104076447618 1108 20-21 0.5162816057185537 1108 22-23 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-1.2300466360171072 2316 90-91 -0.9687309161210784 2316 92-93 -1.1464520573941073 2316 94-95 -0.8059497585801303 2316 96-97 -0.625951615632534 2316 98-99 -0.5402631424050988 2316 100-101 -0.7353310669728899 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 29.0 4 49.0 5 99.0 6 245.0 7 590.0 8 1421.0 9 2401.0 10 3999.0 11 6080.0 12 8185.0 13 10761.0 14 12997.0 15 15821.0 16 18327.0 17 20944.0 18 19204.0 19 17745.0 20 18094.0 21 19634.0 22 21890.0 23 25477.0 24 31325.0 25 39476.0 26 50262.0 27 63782.0 28 81274.0 29 100565.0 30 122212.0 31 147507.0 32 176066.0 33 213027.0 34 259407.0 35 322129.0 36 417278.0 37 528503.0 38 445566.0 39 75769.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.78078183808097 21.724911653758546 14.876618518609497 23.61768798955099 2 15.374208362529709 21.986471477704075 39.52264188529712 23.11667827446909 3 18.0741043950213 26.435044950804265 31.082851988296113 24.40799866587832 4 12.915269720970777 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24-25 21.982675109796567 27.40144838992828 28.819851765158898 21.796024735116255 26-27 21.943571719215136 27.52142295347197 28.71767303457932 21.817332292733568 28-29 21.9464186091639 27.6267828122347 28.688511169864412 21.73828740873699 30-31 22.18923545045339 27.389260920782725 28.626421524854038 21.795082103909845 32-33 22.03392477655031 27.483284393890113 28.520269875820425 21.962520953739148 34-35 22.15660641919962 27.481584313097606 28.622989982205954 21.73881928549682 36-37 22.240001600902083 27.326322210954928 28.611591895493838 21.822084292649148 38-39 22.07435632018716 27.442978570384696 28.598946195832447 21.883718913595697 40-41 22.116486355944616 27.367868741515956 28.673786112804844 21.841858789734584 42-43 22.149805527111436 27.393190007304675 28.426062854147 22.03094161143689 44-45 22.071370287567934 27.506800541799493 28.389559534721954 22.03226963591062 46-47 22.227326466558054 27.474105562039462 28.23959060119609 22.058977370206396 48-49 22.22310832477343 27.52379400137211 28.114772180254484 22.13832549359997 50-51 22.206361759996916 27.565039497664497 28.01093652957932 22.217662212759272 52-53 22.325202420905953 27.524298623296676 27.98174871217031 22.16875024362706 54-55 22.148081971652537 27.562705851777924 28.035022057090973 22.25419011947856 56-57 22.202921490901897 27.678158410032133 27.889177717586183 22.229742381479788 58-59 22.164731013622706 27.62241134140953 27.973631004494603 22.239226640473156 60-61 22.229371415986304 27.741551488301358 27.93994919926502 22.089127896447316 62-63 22.13039577733913 27.70109050605405 27.921774430467643 22.246739286139174 64-65 21.981511112513978 27.603537527118128 27.94079173399408 22.47415962637381 66-67 22.04191506536358 27.618606504746356 27.869237331078388 22.470241098811677 68-69 22.01719402079065 27.66206844221868 27.896107443660366 22.424630093330297 70-71 22.04081038085764 27.42811994141679 27.943166030477595 22.587903647247977 72-73 22.098929199490755 27.5034649614121 27.823943550863326 22.573662288233816 74-75 21.999537715332032 27.708783758216306 27.701702079870692 22.58997644658097 76-77 21.99416334425717 27.56177765911607 27.76662621289106 22.677432783735696 78-79 21.89507890716772 27.606001468801907 27.763297296543342 22.735622327487032 80-81 21.993940217177084 27.660758888803315 27.664715666839896 22.6805852271797 82-83 22.04778663018573 27.513165670202273 27.76873233295767 22.670315366654325 84-85 22.016242084034417 27.580995527658526 27.543853611732967 22.858908776574093 86-87 21.887212298004858 27.624879440391176 27.6373257703308 22.850582491273165 88-89 21.89493491134666 27.474250380250414 27.60080596563746 23.030008742765464 90-91 21.862593637572374 27.47128144562312 27.679759123456808 22.986365793347694 92-93 21.905228625372924 27.326904776778594 27.742164097407247 23.025702500441234 94-95 21.757882278353012 27.239945417232082 27.760940696195597 23.241231608219312 96-97 21.697712755684154 27.13349954717189 27.7988248602782 23.369962836865756 98-99 21.5769636473174 27.258751164664186 27.651909655107804 23.51237553291061 100-101 21.612331841896136 27.169474140286056 27.65150033609639 23.56669368172142 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 370.0 1 254.5 2 203.5 3 286.0 4 266.5 5 260.0 6 411.5 7 837.0 8 1340.0 9 1570.0 10 1410.5 11 1200.5 12 1237.5 13 1369.0 14 1563.0 15 1800.5 16 2063.0 17 2340.0 18 2616.0 19 2958.5 20 3487.5 21 4121.0 22 4904.5 23 6308.0 24 8203.5 25 10960.0 26 14797.5 27 19845.5 28 26723.5 29 36490.0 30 47573.5 31 58708.0 32 70656.0 33 79421.5 34 84818.5 35 89790.0 36 96291.0 37 102933.0 38 105903.5 39 106764.5 40 107104.5 41 107717.5 42 109544.5 43 111967.0 44 114291.0 45 117270.0 46 119942.0 47 121700.0 48 123267.0 49 122356.5 50 118033.0 51 113719.0 52 111403.0 53 109094.0 54 104830.5 55 96220.5 56 85968.5 57 76115.5 58 66585.5 59 57206.0 60 47197.5 61 38260.0 62 30540.0 63 23264.0 64 16850.0 65 12132.5 66 8826.0 67 6697.0 68 4977.5 69 3571.0 70 2574.0 71 1836.0 72 1321.5 73 975.0 74 698.5 75 480.0 76 301.5 77 183.0 78 107.5 79 57.5 80 41.5 81 29.5 82 12.0 83 6.5 84 5.0 85 2.0 86 0.0 87 2.5 88 2.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018919634305299225 2 0.0027894332629607834 3 0.0033655118716157274 4 0.03295776040041708 5 0.0013947166314803917 6 3.0319926771312862E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.5159963385656431E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.547989015696929E-5 24-25 4.851188283410058E-4 26-27 0.0019101553865927103 28-29 3.0319926771312862E-5 30-31 0.006245904914890449 32-33 0.02178486738518829 34-35 0.013007248584893216 36-37 6.215584988119137E-4 38-39 4.5479890156969294E-4 40-41 0.0017585557527361462 42-43 0.008247020081797098 44-45 0.00794382081408397 46-47 0.007640621546370841 48-49 0.00980849631051971 50-51 0.18972694177149024 52-53 0.04931536089354037 54-55 0.017646197380904085 56-57 0.01086969374751566 58-59 0.019768592254895984 60-61 0.05312051170334013 62-63 0.09632640735246097 64-65 0.10057119710044476 66-67 0.10534658556692654 68-69 0.10342127021694816 70-71 0.09153585892259353 72-73 0.0700087109149614 74-75 0.2419681755984623 76-77 0.2921021745148281 78-79 0.007125182791258522 80-81 6.973583157401958E-4 82-83 0.0 84-85 1.5159963385656431E-5 86-87 0.0 88-89 1.6675959724222073E-4 90-91 0.006245904914890449 92-93 0.0176765173076754 94-95 0.0015311563019512996 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3298161.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.304476927888224 #Duplication Level Percentage of deduplicated Percentage of total 1 77.48841182887824 45.17921319655534 2 12.504966663953528 14.581910806849798 3 4.0864417911225015 7.147735533829805 4 1.8565289322003002 4.329757931737175 5 1.0204567615679971 2.9748598855374415 6 0.664869346377619 2.325891567956041 7 0.4455873146730415 1.8185814715399813 8 0.31271793771103396 1.4586284627367814 9 0.24111281001598725 1.2652160641735886 >10 1.289994712408604 13.294892623748572 >50 0.06610230243682015 2.5920394147311963 >100 0.021664491091746958 2.227878890667025 >500 9.369061878096046E-4 0.38854135556922514 >1k 1.5615103114094636E-4 0.25768215632621977 >5k 5.205034371364878E-5 0.15717063804186693 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5179 0.1570269007486293 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4362 0.1322555205764667 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.0319926771312862E-5 2 0.0 0.0 0.0 0.0 3.0319926771312862E-5 3 0.0 0.0 0.0 0.0 3.0319926771312862E-5 4 0.0 0.0 0.0 0.0 3.0319926771312862E-5 5 0.0 0.0 0.0 0.0 3.0319926771312862E-5 6 0.0 0.0 0.0 0.0 6.0639853542625725E-5 7 0.0 0.0 0.0 0.0 6.0639853542625725E-5 8 0.0 0.0 0.0 0.0 6.0639853542625725E-5 9 0.0 0.0 0.0 0.0 6.0639853542625725E-5 10-11 0.0 0.0 0.0 0.0 6.0639853542625725E-5 12-13 0.0 3.0319926771312862E-5 0.0 0.0 4.0931901141272363E-4 14-15 0.0 3.0319926771312862E-5 0.0 0.0 5.002787917266623E-4 16-17 0.0 3.0319926771312862E-5 0.0 0.0 5.457586818836315E-4 18-19 0.0 3.0319926771312862E-5 0.0 0.0 6.063985354262572E-4 20-21 0.0 3.0319926771312862E-5 0.0 4.54798901569693E-5 6.215584988119137E-4 22-23 0.0 3.0319926771312862E-5 0.0 1.2127970708525145E-4 6.670383889688829E-4 24-25 0.0 3.0319926771312862E-5 0.0 3.941590480270672E-4 6.670383889688829E-4 26-27 0.0 3.0319926771312862E-5 0.0 8.641179129824166E-4 7.428382058971651E-4 28-29 0.0 3.0319926771312862E-5 0.0 0.0021223948739919002 7.883180960541344E-4 30-31 0.0 3.0319926771312862E-5 0.0 0.007216142571572461 8.034780594397908E-4 32-33 0.0 4.54798901569693E-5 0.0 0.02161810778794607 8.186380228254473E-4 34-35 0.0 6.0639853542625725E-5 0.0 0.04352425488021962 8.79277876368073E-4 36-37 0.0 7.579981692828215E-5 0.0 0.07681553447512114 8.79277876368073E-4 38-39 0.0 9.095978031393858E-5 0.0 0.12359918148325688 9.095978031393858E-4 40-41 0.0 9.095978031393858E-5 0.0 0.1734148211685239 9.399177299106987E-4 42-43 0.0 9.095978031393858E-5 0.0 0.22715689137067596 9.399177299106987E-4 44-45 0.0 9.095978031393858E-5 0.0 0.2904648984691772 0.001015717546838981 46-47 0.0 9.095978031393858E-5 0.0 0.36784135158956766 0.0011066773271529195 48-49 0.0 9.095978031393858E-5 0.0 0.448264957350475 0.001197637107466858 50-51 0.0 9.095978031393858E-5 0.0 0.5309019177656882 0.0012431169976238274 52-53 0.0 9.095978031393858E-5 0.0 0.6152064741533236 0.0014098765948660481 54-55 0.0 9.095978031393858E-5 0.0 0.7115177215423989 0.0014856764117943302 56-57 0.0 9.095978031393858E-5 0.0 0.8223673738183187 0.0015614762287226125 58-59 0.0 9.095978031393858E-5 0.0 0.9465729538370019 0.001622116082265238 60-61 0.0 9.095978031393858E-5 0.0 1.0729160886930624 0.0016372760456508945 62-63 0.0 9.095978031393858E-5 0.0 1.2052777290132288 0.001758555752736146 64-65 0.0 9.095978031393858E-5 0.0 1.3482968235935116 0.0017888756795074589 66-67 0.0 9.095978031393858E-5 0.0 1.5106751914172776 0.0018343555696644282 68-69 3.0319926771312862E-5 9.095978031393858E-5 0.0 1.6955812648321291 0.0018495155330500847 70-71 3.0319926771312862E-5 9.095978031393858E-5 0.0 1.8931004277838468 0.0018798354598213974 72-73 3.0319926771312862E-5 9.095978031393858E-5 0.0 2.1038997186613995 0.0019253153499783667 74-75 3.0319926771312862E-5 9.095978031393858E-5 0.0 2.3250077846411985 0.0019859552035209928 76-77 3.0319926771312862E-5 9.095978031393858E-5 0.0 2.5702505123309627 0.002001115166906649 78-79 3.0319926771312862E-5 1.2127970708525145E-4 0.0 2.8455099675243263 0.002001115166906649 80-81 3.0319926771312862E-5 1.2127970708525145E-4 0.0 3.14698099941149 0.002031435093677962 82-83 3.0319926771312862E-5 1.2127970708525145E-4 0.0 3.4636726345378532 0.002076914983834931 84-85 3.0319926771312862E-5 1.3643967047090788E-4 0.0 3.789536047512538 0.0020920749472205873 86-87 3.0319926771312862E-5 1.515996338565643E-4 0.0 4.15470924554623 0.002243674581077152 88-89 3.0319926771312862E-5 1.515996338565643E-4 0.0 4.551218087898074 0.0023801142515480593 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3575 0.0 52.464645 1 GTATCAA 6390 0.0 39.45839 1 CAACGCA 7925 0.0 31.438515 5 ATCAACG 7945 0.0 31.369835 3 TCAACGC 7975 0.0 31.191359 4 AACGCAG 8465 0.0 29.71285 6 TATCAAC 8510 0.0 29.566936 2 ACGCAGA 9930 0.0 24.994698 7 GTACATG 8345 0.0 24.524298 1 CGCAGAG 10195 0.0 24.112263 8 TACATGG 8805 0.0 22.699316 2 ACATGGG 8590 0.0 22.603569 3 GTGGTAT 1975 0.0 21.878622 1 GCAGAGT 11765 0.0 20.692875 9 CATGGGG 6075 0.0 19.691916 4 TGGTATC 1965 0.0 19.569628 2 AGAGTAC 10765 0.0 18.801844 10-11 CAGAGTA 11260 0.0 18.14388 10-11 GAGTACA 6470 0.0 18.054127 1 GAGTACT 7550 0.0 17.568321 12-13 >>END_MODULE