FastQCFastQC Report
Sat 18 Jun 2016
SRR1930945_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930945_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1787516
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33390.18679553078126296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16000.061.1806031
GTATCAA30550.045.4206661
ATCAACG40050.033.8728753
TCAACGC40400.033.561684
TATCAAC41000.033.217292
CAACGCA41050.032.8829045
AACGCAG44700.030.1708556
TACATGG35850.025.4667632
CGCAGAG54500.025.3783178
GTACATG35600.025.3708521
ACGCAGA53400.025.2673667
ACATGGG36750.024.0150243
GCAGAGT57100.023.0190879
GAGTACA25650.021.048851
AGAGTAC52100.020.22699510-11
CATGGGG26900.019.8621544
GTGGTAT7200.019.6226441
AGTACAT27450.019.29442
GAGTACT40050.018.48876812-13
CAGAGTA56450.018.39435810-11
Produced by FastQC (version 0.11.5)