##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930945_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1787516 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.603603548164045 30.0 10.0 31.0 2.0 33.0 2 21.49996531499578 28.0 10.0 31.0 2.0 33.0 3 21.94118094607265 28.0 10.0 31.0 2.0 33.0 4 23.849561626301526 32.0 2.0 35.0 2.0 37.0 5 23.834355608565183 32.0 10.0 35.0 2.0 37.0 6 24.58861123480853 32.0 11.0 35.0 2.0 37.0 7 24.671250495100463 32.0 11.0 35.0 2.0 37.0 8 25.453948384238238 32.0 17.0 35.0 2.0 37.0 9 26.582440660671008 33.0 17.0 37.0 2.0 39.0 10-11 26.831608220569773 33.0 17.0 37.0 2.0 39.0 12-13 26.767282922222794 33.0 17.0 37.0 2.0 39.0 14-15 26.969187968107697 33.5 16.5 38.0 2.0 40.0 16-17 26.079404883648593 33.0 10.5 38.0 2.0 40.0 18-19 25.48640515665314 32.0 10.0 38.0 2.0 40.0 20-21 24.98480461153914 32.0 10.0 38.0 2.0 40.0 22-23 24.91939344878591 32.0 10.0 38.0 2.0 40.0 24-25 24.612958988898562 32.0 8.5 38.0 2.0 40.0 26-27 24.285245838358932 31.0 5.0 37.0 2.0 39.5 28-29 24.1768031726709 31.0 5.0 37.0 2.0 39.0 30-31 24.06151021864979 31.0 2.0 37.0 2.0 39.0 32-33 23.73736458862466 30.0 2.0 37.0 2.0 39.0 34-35 20.865010718785175 25.0 2.0 36.0 2.0 39.0 36-37 19.24113490452673 18.0 2.0 35.0 2.0 38.5 38-39 18.33453350907069 17.0 2.0 34.0 2.0 38.0 40-41 19.613788352104258 22.0 2.0 34.5 2.0 38.0 42-43 19.51257583148906 21.5 2.0 35.0 2.0 38.0 44-45 17.57022258821739 15.5 2.0 33.5 2.0 38.0 46-47 18.369778787994065 18.5 2.0 33.0 2.0 38.0 48-49 17.791260329977465 15.0 2.0 33.0 2.0 38.0 50-51 15.871020734919295 10.0 2.0 31.0 2.0 37.5 52-53 18.733452735527962 21.0 2.0 33.5 2.0 37.5 54-55 20.311379310730644 26.0 2.0 35.5 2.0 38.5 56-57 20.510321306214884 26.5 2.0 36.0 2.0 39.0 58-59 20.14160796322942 26.0 2.0 35.5 2.0 39.0 60-61 19.82628826818893 26.0 2.0 35.0 2.0 39.0 62-63 19.350545393719553 24.0 2.0 34.5 2.0 38.0 64-65 18.853791798227263 22.5 2.0 34.0 2.0 38.0 66-67 18.437244198093893 22.0 2.0 34.0 2.0 38.0 68-69 17.97442456459131 20.5 2.0 33.5 2.0 37.0 70-71 17.39245662696166 18.0 2.0 33.0 2.0 36.0 72-73 16.910392410473527 14.0 2.0 32.0 2.0 36.0 74-75 16.381590150801447 8.0 2.0 32.0 2.0 35.5 76-77 15.818427359531327 7.0 2.0 31.0 2.0 35.0 78-79 15.31610290481316 2.0 2.0 31.0 2.0 35.0 80-81 14.811213158371729 2.0 2.0 31.0 2.0 34.0 82-83 14.330896898265525 2.0 2.0 30.0 2.0 34.0 84-85 13.809523663004974 2.0 2.0 30.0 2.0 34.0 86-87 13.249697904801971 2.0 2.0 29.0 2.0 34.0 88-89 12.701588125644749 2.0 2.0 29.0 2.0 34.0 90-91 12.136586469715516 2.0 2.0 28.5 2.0 34.0 92-93 11.519322903962818 2.0 2.0 26.5 2.0 33.5 94-95 10.873543509540614 2.0 2.0 25.5 2.0 33.0 96-97 10.159391020835617 2.0 2.0 24.5 2.0 32.5 98-99 9.384405230498636 2.0 2.0 19.0 2.0 32.0 100-101 8.381973923590055 2.0 2.0 2.0 2.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.780101053913292 1101 2 7.807534151909657 1101 3 7.6593307234079475 1101 4 9.412642648970891 1101 5 9.234337483088826 1101 6 8.648155815088103 1101 7 8.529725868457827 1101 8 7.410605795560933 1101 9 7.690527144139605 1101 10-11 7.22749168230521 1101 12-13 7.126503048744919 1101 14-15 7.460767130765234 1101 16-17 8.17302656604841 1101 18-19 8.742621599240646 1101 20-21 9.196333687678521 1101 22-23 8.988180747033915 1101 24-25 8.987659655519092 1101 26-27 9.00903426713235 1101 28-29 8.84698705427384 1101 30-31 8.554836069766584 1101 32-33 8.675461426495833 1101 34-35 11.449637366889242 1101 36-37 5.732118174014275 1101 38-39 8.811870748266525 1101 40-41 10.242616178206521 1101 42-43 10.509608178102543 1101 44-45 4.835244447869908 1101 46-47 6.460842292787493 1101 48-49 9.22889986049157 1101 50-51 -3.2662983776759535 1101 52-53 5.901520842828411 1101 54-55 8.526931732342323 1101 56-57 9.213002140534265 1101 58-59 9.189395427859878 1101 60-61 9.130604310048046 1101 62-63 9.202711480371487 1101 64-65 8.938358137928518 1101 66-67 9.03739584664531 1101 68-69 8.995057459519987 1101 70-71 8.889330885901575 1101 72-73 8.522920709823058 1101 74-75 8.707285145585512 1101 76-77 8.3633805494647 1101 78-79 8.575889186973782 1101 80-81 8.386986184731944 1101 82-83 8.189479172502677 1101 84-85 8.067486354119753 1101 86-87 7.6524121518621016 1101 88-89 7.479448499399718 1101 90-91 7.287054967921145 1101 92-93 7.115206136544316 1101 94-95 7.072515555475986 1101 96-97 6.615028740088235 1101 98-99 6.235674543784642 1101 100-101 5.811346152140301 1102 1 6.5411741802873635 1102 2 7.64413647473388 1102 3 7.454705218383605 1102 4 9.295269811511037 1102 5 9.06388960850752 1102 6 8.400898197997272 1102 7 8.163647965215308 1102 8 7.325741562219548 1102 9 7.0437938315252175 1102 10-11 6.791818258959562 1102 12-13 6.529577222576886 1102 14-15 6.764721284338677 1102 16-17 7.659585148872399 1102 18-19 7.972180343211999 1102 20-21 8.384946611697533 1102 22-23 8.269113847854612 1102 24-25 8.33911919026362 1102 26-27 8.310769766746745 1102 28-29 8.229530765497756 1102 30-31 8.07575789027144 1102 32-33 7.949151528594204 1102 34-35 10.138752437621424 1102 36-37 1.0428146184126739 1102 38-39 3.3857309183921025 1102 40-41 8.240354359773566 1102 42-43 9.275171848579191 1102 44-45 1.2830801811479908 1102 46-47 1.7181327724311295 1102 48-49 7.205650036027098 1102 50-51 -4.406164222491038 1102 52-53 4.538825595524749 1102 54-55 7.104370237355155 1102 56-57 7.7198350767544355 1102 58-59 8.088430845749542 1102 60-61 8.027133310819252 1102 62-63 7.7837642906665 1102 64-65 7.768983619931809 1102 66-67 7.606336705560789 1102 68-69 7.6773899525848925 1102 70-71 7.44440587698092 1102 72-73 7.246098305734524 1102 74-75 7.275856228841146 1102 76-77 7.35201102703644 1102 78-79 7.294938820363516 1102 80-81 7.128579184029801 1102 82-83 7.005031620216114 1102 84-85 6.865066553712277 1102 86-87 6.6723383118353965 1102 88-89 6.3763069889884445 1102 90-91 6.388182136216551 1102 92-93 6.638345172192416 1102 94-95 6.205860687098857 1102 96-97 5.770720119855035 1102 98-99 5.574265310040383 1102 100-101 5.230617075399621 1103 1 6.848048092643001 1103 2 7.84270737596259 1103 3 7.456221328703702 1103 4 9.1244371373561 1103 5 9.077336847868384 1103 6 8.419988004462496 1103 7 8.300005609048032 1103 8 7.658613470672847 1103 9 7.498798313938341 1103 10-11 7.009202111725482 1103 12-13 6.789616509609527 1103 14-15 7.14212069371483 1103 16-17 7.786041933136492 1103 18-19 8.31536839362597 1103 20-21 8.724693750863285 1103 22-23 8.45657759304261 1103 24-25 8.671727427356902 1103 26-27 8.574302699299608 1103 28-29 8.464580599384803 1103 30-31 8.349231233096948 1103 32-33 8.297059296352469 1103 34-35 10.37699269496467 1103 36-37 -2.993842292413671 1103 38-39 -1.4903962766719694 1103 40-41 6.7308753743809575 1103 42-43 9.109276420640573 1103 44-45 1.158647074572027 1103 46-47 1.0351382567954168 1103 48-49 7.840959586203759 1103 50-51 -4.696815754421639 1103 52-53 4.962842101351889 1103 54-55 7.8420633766263705 1103 56-57 8.268357198781544 1103 58-59 8.504385599744161 1103 60-61 8.303039021940908 1103 62-63 8.352628658118906 1103 64-65 8.321533321983171 1103 66-67 8.244441172153628 1103 68-69 8.317228058369846 1103 70-71 8.209804284405902 1103 72-73 8.00528725263111 1103 74-75 7.9390028828515895 1103 76-77 7.815967152122134 1103 78-79 7.297753512131695 1103 80-81 7.287065446751944 1103 82-83 7.051147741083065 1103 84-85 6.888131205929755 1103 86-87 6.860487602559438 1103 88-89 6.809987050160201 1103 90-91 6.75876564093627 1103 92-93 6.624805647855549 1103 94-95 6.236242219556802 1103 96-97 6.060481760945578 1103 98-99 5.840599750793427 1103 100-101 5.37307871121674 1104 1 6.459219031939529 1104 2 7.65177529471352 1104 3 7.0720705329589215 1104 4 9.00460876048879 1104 5 9.092147913450244 1104 6 8.17097651565605 1104 7 7.897058293149829 1104 8 6.977544963212573 1104 9 7.316094702170613 1104 10-11 6.463260795995989 1104 12-13 6.400662258428067 1104 14-15 6.957606082774525 1104 16-17 7.634214600869303 1104 18-19 8.057679723947555 1104 20-21 8.282045348545058 1104 22-23 8.185692770077203 1104 24-25 7.875316759512817 1104 26-27 8.15655867658404 1104 28-29 8.159973769279496 1104 30-31 7.792354820777842 1104 32-33 7.709583010749466 1104 34-35 10.097562163564746 1104 36-37 -3.052283361351897 1104 38-39 -1.8358012925473766 1104 40-41 6.345666068465686 1104 42-43 8.535934095130973 1104 44-45 0.4987528089241131 1104 46-47 0.2633147296008147 1104 48-49 6.903099383134929 1104 50-51 -5.041499691092309 1104 52-53 3.9445885219811245 1104 54-55 6.484857251177431 1104 56-57 7.265786979936024 1104 58-59 7.56669411215821 1104 60-61 7.504473193287634 1104 62-63 7.62727368253778 1104 64-65 7.495028927303814 1104 66-67 7.374168122298201 1104 68-69 7.3854775095958125 1104 70-71 7.287771478218712 1104 72-73 7.173232746761066 1104 74-75 7.13012970484419 1104 76-77 6.79485034536777 1104 78-79 6.842593241397193 1104 80-81 6.484065003425092 1104 82-83 6.30725380568674 1104 84-85 6.071769034812769 1104 86-87 5.708617193998741 1104 88-89 5.833127993997687 1104 90-91 5.715994919892962 1104 92-93 5.603726084146608 1104 94-95 5.383745320152508 1104 96-97 5.027665253391971 1104 98-99 4.412402996233174 1104 100-101 4.100659744006773 1105 1 5.887426071972762 1105 2 6.917952120502626 1105 3 6.689855794092651 1105 4 8.412439213469082 1105 5 8.279629900304585 1105 6 7.448181762250908 1105 7 7.327191208286475 1105 8 6.260338223058106 1105 9 6.413959320326935 1105 10-11 5.9548258305987325 1105 12-13 5.624204958480465 1105 14-15 6.10407556456768 1105 16-17 6.735700080086065 1105 18-19 7.5528934779979515 1105 20-21 7.883498156145411 1105 22-23 7.513215952339316 1105 24-25 7.446413259799279 1105 26-27 7.287882822192106 1105 28-29 7.298915214741818 1105 30-31 7.203709286812778 1105 32-33 6.945118381858187 1105 34-35 8.894332603453055 1105 36-37 -3.7324194853080073 1105 38-39 -2.275293750654779 1105 40-41 5.031744926079281 1105 42-43 7.506524972982302 1105 44-45 -3.2801321088075888 1105 46-47 -4.334152432507578 1105 48-49 4.405735865895402 1105 50-51 -9.50438065089105 1105 52-53 0.27230671295372133 1105 54-55 4.79498906948124 1105 56-57 6.021934786328693 1105 58-59 6.39605011375912 1105 60-61 6.0837663311980315 1105 62-63 6.20863928025917 1105 64-65 6.132343938861396 1105 66-67 5.913786395299894 1105 68-69 5.7077328312770454 1105 70-71 5.695694549397327 1105 72-73 5.643832813504513 1105 74-75 5.801100127696586 1105 76-77 5.5948067054229895 1105 78-79 5.409529653096897 1105 80-81 5.200225085010784 1105 82-83 5.099628746323576 1105 84-85 4.983337908330439 1105 86-87 4.891473230021532 1105 88-89 4.745430520330428 1105 90-91 4.321961515498861 1105 92-93 4.030009145197024 1105 94-95 3.99974472739604 1105 96-97 3.7082296088918287 1105 98-99 3.268932593800537 1105 100-101 3.1282456732750568 1106 1 5.853193148458573 1106 2 7.2447290231887855 1106 3 6.549268834584343 1106 4 8.216930933380706 1106 5 8.150289131820497 1106 6 7.292319486707402 1106 7 7.035021088673936 1106 8 6.319629871801286 1106 9 6.249584420266803 1106 10-11 6.0030708865312725 1106 12-13 5.885580086009366 1106 14-15 6.089524319908136 1106 16-17 6.375989472158896 1106 18-19 6.746732621544265 1106 20-21 7.176816035866235 1106 22-23 6.951014223576678 1106 24-25 7.314022777002446 1106 26-27 7.175967062209942 1106 28-29 7.25012513463227 1106 30-31 6.799658034270646 1106 32-33 6.684124714949039 1106 34-35 9.200775543700836 1106 36-37 -0.0014547925147141427 1106 38-39 1.4235927784645988 1106 40-41 6.802865739021126 1106 42-43 8.056944776728553 1106 44-45 -3.0443421503150088 1106 46-47 -4.060259484715038 1106 48-49 4.968868583786833 1106 50-51 -9.37211544482912 1106 52-53 0.6138189748716201 1106 54-55 5.023124478711068 1106 56-57 6.127280414987055 1106 58-59 6.6643531854883555 1106 60-61 6.481277012095383 1106 62-63 6.417247002486018 1106 64-65 6.2852249096350405 1106 66-67 6.21149543571952 1106 68-69 5.839455265327995 1106 70-71 5.95733684012043 1106 72-73 5.9461413399798495 1106 74-75 5.905778270650572 1106 76-77 5.668825830816836 1106 78-79 5.552831880263874 1106 80-81 5.2588343274497 1106 82-83 5.425112061151614 1106 84-85 5.000920286933061 1106 86-87 4.616598619364737 1106 88-89 4.697125218956829 1106 90-91 4.648351045629466 1106 92-93 4.481936379965061 1106 94-95 4.164595397528039 1106 96-97 3.793383443091871 1106 98-99 3.709649390679937 1106 100-101 3.394962375563482 1107 1 6.05081078012709 1107 2 7.342963973212946 1107 3 6.735542808565846 1107 4 8.058034768730732 1107 5 8.35756805549607 1107 6 6.952602290808933 1107 7 6.995173535138832 1107 8 6.577294267716205 1107 9 6.46224972798171 1107 10-11 6.105327090964945 1107 12-13 5.825717846271441 1107 14-15 5.993865813625916 1107 16-17 6.982052779758003 1107 18-19 7.085612422495895 1107 20-21 7.626199772709395 1107 22-23 7.554941835856596 1107 24-25 7.658905035755591 1107 26-27 7.377698345824115 1107 28-29 7.271435280287584 1107 30-31 7.193996649189639 1107 32-33 7.015018690196779 1107 34-35 9.19565918232934 1107 36-37 -0.11612338162733238 1107 38-39 1.218457937585871 1107 40-41 6.968121370400684 1107 42-43 7.938169299765168 1107 44-45 -3.0599446174239215 1107 46-47 -3.8833889554435537 1107 48-49 4.97086805023503 1107 50-51 -9.37902997318502 1107 52-53 0.7582838078011847 1107 54-55 5.280225019676319 1107 56-57 6.41125456965106 1107 58-59 6.782841458982901 1107 60-61 6.812032999807457 1107 62-63 6.5138178498864185 1107 64-65 6.583177607507814 1107 66-67 6.6512055699029276 1107 68-69 6.4091313950535955 1107 70-71 6.364309160903034 1107 72-73 6.212620704349735 1107 74-75 6.038240217205566 1107 76-77 6.307801871310495 1107 78-79 5.985008300845557 1107 80-81 5.8553487622987515 1107 82-83 5.640859225778319 1107 84-85 5.605833427837817 1107 86-87 5.422085166121136 1107 88-89 5.35565274043554 1107 90-91 5.274121678959785 1107 92-93 5.081252502915746 1107 94-95 5.041321121056212 1107 96-97 4.781580232798326 1107 98-99 4.451974307495098 1107 100-101 4.185389747312184 1108 1 5.97043566123757 1108 2 6.349808306832003 1108 3 6.5697990727517634 1108 4 8.02705496433681 1108 5 8.207083627329109 1108 6 7.449129418067834 1108 7 7.64751957562731 1108 8 6.898628118332937 1108 9 5.5208989440707725 1108 10-11 5.990799661957393 1108 12-13 5.464043074945518 1108 14-15 6.173559207154227 1108 16-17 6.267430736521735 1108 18-19 6.772093226363136 1108 20-21 7.436223299156335 1108 22-23 7.845953263066917 1108 24-25 7.4493899226247215 1108 26-27 7.758918941219516 1108 28-29 7.653918411930132 1108 30-31 7.379878100655912 1108 32-33 7.2605471731139914 1108 34-35 9.604595750452411 1108 36-37 -3.358740625623172 1108 38-39 -2.0205088938267153 1108 40-41 5.51749098652224 1108 42-43 7.819707394426924 1108 44-45 -2.9260935309353524 1108 46-47 -3.872333818891237 1108 48-49 5.330593890033025 1108 50-51 -9.428826375744245 1108 52-53 0.696835817717993 1108 54-55 5.645576869102637 1108 56-57 6.884218774855615 1108 58-59 7.139706627627788 1108 60-61 7.208932228469379 1108 62-63 7.133719554987351 1108 64-65 6.932888265922259 1108 66-67 7.1037059940642635 1108 68-69 7.047381186565175 1108 70-71 6.781267352135412 1108 72-73 6.836909923246278 1108 74-75 6.937214382326186 1108 76-77 6.796191041960409 1108 78-79 6.749722277942503 1108 80-81 6.714293288627976 1108 82-83 6.582235984389898 1108 84-85 6.2483345041299305 1108 86-87 6.139412308633261 1108 88-89 5.777364163722021 1108 90-91 5.581145956462434 1108 92-93 5.39145674696077 1108 94-95 5.097767875959489 1108 96-97 4.767249935189049 1108 98-99 4.590467957233445 1108 100-101 4.155721941627263 1109 1 5.010661348849986 1109 2 6.631089641525996 1109 3 6.314236365567599 1109 4 7.972056531766292 1109 5 7.789998423641091 1109 6 5.23045855380278 1109 7 4.909793326630577 1109 8 3.362541111545191 1109 9 2.8823487385262965 1109 10-11 2.8093457988551336 1109 12-13 3.4014817951226775 1109 14-15 3.918199098568053 1109 16-17 3.412044448528242 1109 18-19 4.097518822375417 1109 20-21 4.462050034834366 1109 22-23 6.018803772537083 1109 24-25 5.928864100057734 1109 26-27 4.7177803093075426 1109 28-29 4.602704043159552 1109 30-31 4.678498151302787 1109 32-33 4.42317798677664 1109 34-35 6.479986774083912 1109 36-37 -4.43995904080745 1109 38-39 -3.1302178410699266 1109 40-41 3.1570819763595175 1109 42-43 4.289707516708656 1109 44-45 -4.363807940837059 1109 46-47 -5.76230999618641 1109 48-49 1.7595084617912775 1109 50-51 -9.772506966965306 1109 52-53 -1.0740879763062097 1109 54-55 2.382648632916581 1109 56-57 3.98816325005577 1109 58-59 4.660313878712202 1109 60-61 5.069091116906197 1109 62-63 4.985723919333555 1109 64-65 4.6341109165011005 1109 66-67 4.781395525191634 1109 68-69 4.574984620236226 1109 70-71 4.38264585634079 1109 72-73 4.410099153115922 1109 74-75 4.42392902789814 1109 76-77 4.312985326289894 1109 78-79 4.382679483782674 1109 80-81 4.279916058375923 1109 82-83 4.326092716888601 1109 84-85 4.08829356198253 1109 86-87 3.980908225535046 1109 88-89 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-11.058936151240705 2313 88-89 -10.49713676218506 2313 90-91 -9.950005899082495 2313 92-93 -9.342550158338948 2313 94-95 -8.785352464828582 2313 96-97 -8.050694008428977 2313 98-99 -7.253646427903203 2313 100-101 -6.2661440494324765 2314 1 -20.50134282651536 2314 2 -19.388743343860224 2314 3 -19.789625847376033 2314 4 -21.69339232000824 2314 5 -21.691212432308806 2314 6 -22.44490679066486 2314 7 -22.49678287378696 2314 8 -23.28933780285982 2314 9 -24.438632472329655 2314 10-11 -24.658295119725242 2314 12-13 -24.598257244543294 2314 14-15 -24.780913636296248 2314 16-17 -23.91467788967635 2314 18-19 -23.306447774701827 2314 20-21 -22.82735497560405 2314 22-23 -22.741373680490057 2314 24-25 -22.45396470996314 2314 26-27 -22.131123657289535 2314 28-29 -22.020469234502244 2314 30-31 -21.879174082517675 2314 32-33 -21.578909695895593 2314 34-35 -18.783583163285606 2314 36-37 -17.164384928072586 2314 38-39 -16.275833707458236 2314 40-41 -17.53628963115614 2314 42-43 -17.429760124209924 2314 44-45 -15.496114830189878 2314 46-47 -16.245869495142568 2314 48-49 -15.714134544471769 2314 50-51 -13.797037238364053 2314 52-53 -16.6213217967655 2314 54-55 -18.151015250173188 2314 56-57 -18.348475580841935 2314 58-59 -17.965990922957943 2314 60-61 -17.64463539666374 2314 62-63 -17.183639337451414 2314 64-65 -16.69756966805005 2314 66-67 -16.252790278566195 2314 68-69 -15.79766673675751 2314 70-71 -15.2387006989828 2314 72-73 -14.775443644378857 2314 74-75 -14.232647172519396 2314 76-77 -13.68597083237399 2314 78-79 -13.191172290747595 2314 80-81 -12.687463899870425 2314 82-83 -12.214356134885927 2314 84-85 -11.702677210460058 2314 86-87 -11.142004091792721 2314 88-89 -10.582593237768924 2314 90-91 -10.03387001797846 2314 92-93 -9.420044850483322 2314 94-95 -8.785352464828582 2314 96-97 -8.06714836087059 2314 98-99 -7.294782309007237 2314 100-101 -6.310199251130991 2315 1 -20.50134282651536 2315 2 -19.388743343860224 2315 3 -19.789625847376033 2315 4 -16.765497583166134 2315 5 -21.691212432308806 2315 6 -22.44490679066486 2315 7 -22.49678287378696 2315 8 -23.28933780285982 2315 9 -19.824421946013864 2315 10-11 -18.126453014462086 2315 12-13 -14.852994086648557 2315 14-15 -14.758018899454143 2315 16-17 -19.9507305212553 2315 18-19 -23.306447774701827 2315 20-21 -22.82735497560405 2315 22-23 -22.741373680490057 2315 24-25 -22.45396470996314 2315 26-27 -22.131123657289535 2315 28-29 -22.020469234502244 2315 30-31 -21.879174082517675 2315 32-33 -21.578909695895593 2315 34-35 -18.783583163285606 2315 36-37 -17.164384928072586 2315 38-39 -16.275833707458236 2315 40-41 -17.53628963115614 2315 42-43 -17.429760124209924 2315 44-45 -15.496114830189878 2315 46-47 -16.245869495142568 2315 48-49 -15.714134544471769 2315 50-51 -13.797037238364053 2315 52-53 -16.6213217967655 2315 54-55 -18.151015250173188 2315 56-57 -18.348475580841935 2315 58-59 -17.965990922957943 2315 60-61 -17.64463539666374 2315 62-63 -17.183639337451414 2315 64-65 -16.69756966805005 2315 66-67 -16.252790278566195 2315 68-69 -15.79766673675751 2315 70-71 -15.2387006989828 2315 72-73 -14.775443644378857 2315 74-75 -14.232647172519396 2315 76-77 -13.68597083237399 2315 78-79 -13.191172290747595 2315 80-81 -12.687463899870425 2315 82-83 -12.214356134885927 2315 84-85 -11.702677210460058 2315 86-87 -11.142004091792721 2315 88-89 -10.582593237768924 2315 90-91 -10.03387001797846 2315 92-93 -9.420044850483322 2315 94-95 -8.785352464828582 2315 96-97 -8.06714836087059 2315 98-99 -7.294782309007237 2315 100-101 -6.310199251130991 2316 1 -20.50134282651536 2316 2 -19.388743343860224 2316 3 -19.789625847376033 2316 4 -21.69339232000824 2316 5 -21.691212432308806 2316 6 -22.44490679066486 2316 7 -22.49678287378696 2316 8 -23.28933780285982 2316 9 -24.438632472329655 2316 10-11 -24.658295119725242 2316 12-13 -24.598257244543294 2316 14-15 -24.780913636296248 2316 16-17 -23.91467788967635 2316 18-19 -23.306447774701827 2316 20-21 -22.82735497560405 2316 22-23 -22.741373680490057 2316 24-25 -22.45396470996314 2316 26-27 -22.131123657289535 2316 28-29 -22.020469234502244 2316 30-31 -21.879174082517675 2316 32-33 -21.578909695895593 2316 34-35 -18.783583163285606 2316 36-37 -17.164384928072586 2316 38-39 -16.275833707458236 2316 40-41 -17.53628963115614 2316 42-43 -17.429760124209924 2316 44-45 -15.496114830189878 2316 46-47 -16.245869495142568 2316 48-49 -15.714134544471769 2316 50-51 -13.797037238364053 2316 52-53 -16.6213217967655 2316 54-55 -18.151015250173188 2316 56-57 -18.348475580841935 2316 58-59 -17.965990922957943 2316 60-61 -17.64463539666374 2316 62-63 -17.183639337451414 2316 64-65 -16.69756966805005 2316 66-67 -16.252790278566195 2316 68-69 -15.79766673675751 2316 70-71 -15.2387006989828 2316 72-73 -14.775443644378857 2316 74-75 -14.232647172519396 2316 76-77 -13.68597083237399 2316 78-79 -13.191172290747595 2316 80-81 -12.687463899870425 2316 82-83 -12.214356134885927 2316 84-85 -11.702677210460058 2316 86-87 -11.142004091792721 2316 88-89 -10.582593237768924 2316 90-91 -10.03387001797846 2316 92-93 -9.420044850483322 2316 94-95 -8.785352464828582 2316 96-97 -8.06714836087059 2316 98-99 -7.294782309007237 2316 100-101 -6.310199251130991 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 305542.0 3 18857.0 4 38632.0 5 23546.0 6 21260.0 7 25831.0 8 33300.0 9 38767.0 10 40041.0 11 37588.0 12 33674.0 13 31050.0 14 30009.0 15 31391.0 16 34258.0 17 36139.0 18 37281.0 19 38104.0 20 40448.0 21 42512.0 22 45123.0 23 47247.0 24 50232.0 25 52423.0 26 55749.0 27 59210.0 28 62090.0 29 65023.0 30 67232.0 31 66853.0 32 64274.0 33 62587.0 34 57393.0 35 47799.0 36 32103.0 37 12513.0 38 1432.0 39 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.39050402539189 20.96215691974348 14.237531259207154 23.40980779565747 2 15.33819060709244 21.36790235463059 40.617145179443185 22.676761858833782 3 18.304410421746663 26.20552839438829 31.03116998367616 24.458891200188884 4 12.43920743976941 17.33110962057901 39.24891684643692 30.98076609321466 5 12.906652416453781 37.838514965619915 36.00604475493689 13.248787862989417 6 30.058907102360305 38.088233323057544 18.25329383854495 13.599565736037208 7 25.99567924936045 33.14381861042069 23.460988670751266 17.3995134694676 8 24.918169993346428 35.81790774181078 21.392031916732744 17.871890348110046 9 25.92003676682856 16.131619241614878 21.557382349612816 36.39096164194375 10-11 24.274867672864307 26.591191855168912 29.427715103512426 19.706225368454362 12-13 24.87886919615134 24.451336757425132 29.3964987398448 21.27329530657873 14-15 22.130670603080187 25.81109816733955 27.24907785718956 24.809153372390693 16-17 21.624919051689954 28.7572457256281 27.86948485652457 21.74835036615738 18-19 21.54986851540384 27.69628865749002 30.01953372755708 20.734309099549055 20-21 22.174327667437588 26.934743357244656 30.03603868958385 20.85489028573391 22-23 21.990769694992448 26.804280911785984 29.78839803014614 21.416551363075428 24-25 21.799091494111124 27.085674836220768 29.73265421859225 21.382579451075863 26-27 21.718314047337152 27.225393463026133 29.64843358191891 21.407858907717806 28-29 21.739015434855467 27.441244749113846 29.632464580408108 21.18727523562258 30-31 21.96688455886284 27.31306714235232 29.404819185988135 21.3152291127967 32-33 21.860610742399654 27.30608286931821 29.310442525245378 21.522863863036758 34-35 22.21735114224894 27.275293623563172 29.29817858306271 21.209176651125176 36-37 22.663113158489004 27.250346770113126 28.835861407267593 21.250678664130277 38-39 22.478225266135638 27.268776192081834 28.909124879759098 21.343873662023434 40-41 22.42626775543814 27.192788032663145 29.066321279077744 21.31462293282097 42-43 22.487744551666992 27.24019032555217 28.698429203737096 21.573635919043735 44-45 22.75577552910849 27.356710946824176 28.44979323040607 21.437720293661265 46-47 22.134272975064345 27.358084871613624 28.7055527444414 21.802089408880633 48-49 22.6507810268565 27.466117085664916 28.163497007394 21.719604880084585 50-51 22.79951178729791 27.66936928968048 27.95998567605047 21.57113324697114 52-53 21.93160983402723 27.771140609791654 28.452316427844565 21.844933128336546 54-55 21.733455763902313 27.65870867968297 28.634512533024036 21.97332302339068 56-57 21.782428467087815 27.83514434720211 28.371721274714574 22.0107059109955 58-59 21.85156593724579 27.788565320253838 28.517964581484602 21.841904161015773 60-61 21.744595670612085 27.867114302705552 28.462645820058423 21.925644206623936 62-63 21.8007419409035 27.75870248530248 28.448502825567928 21.992052748226094 64-65 21.5552873780858 27.631842667093377 28.488203649465905 22.32466630535492 66-67 21.66580911139164 27.72745721544878 28.347933078729966 22.25880059442962 68-69 21.68443499560698 27.751136426864313 28.292176543735454 22.27225203379325 70-71 21.684224730486743 27.558235677145493 28.232265464430995 22.52527412793677 72-73 21.81376807793962 27.69326469787276 28.136000065712114 22.356967158475506 74-75 21.77502792087799 27.874111306595907 27.980514199489768 22.370346573036336 76-77 21.84008557183773 27.73646860515184 27.962072263404846 22.461373559605587 78-79 21.72978241732599 27.849285189048413 27.951144438259274 22.469787955366318 80-81 21.842511727241522 27.83983575505638 27.853056710270398 22.464595807431703 82-83 21.97579568128575 27.76573813362641 27.893916867827823 22.364549317260018 84-85 21.946059278803375 27.831151317339685 27.75131727726599 22.471472126590946 86-87 21.774447126219066 27.908834665781495 27.695636708931186 22.621081499068254 88-89 21.875688021367303 27.729838452185827 27.589478121992762 22.804995404454107 90-91 21.899635801474453 27.698937039156746 27.635129753827975 22.76629740554083 92-93 21.99350329147401 27.55358141876919 27.658332393543976 22.794582896212823 94-95 21.8385726938945 27.53216963062911 27.66463328059874 22.96462439487765 96-97 21.83032154254859 27.44529152425607 27.501977888543035 23.22240904465231 98-99 21.816574804979755 27.423320514157425 27.400234690172535 23.35986999069029 100-101 22.088753355151777 27.554726393189828 27.29665956435225 23.059860687306145 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 22266.0 1 24027.0 2 18039.0 3 11085.5 4 14095.5 5 17465.0 6 18467.5 7 17829.0 8 16827.5 9 15360.5 10 13597.0 11 11850.5 12 10000.0 13 8547.5 14 7461.0 15 6487.5 16 5783.5 17 5426.5 18 5343.5 19 5511.5 20 5972.5 21 6621.5 22 7501.5 23 8654.5 24 10304.5 25 12305.0 26 14482.5 27 17276.5 28 20424.5 29 23934.5 30 27919.0 31 31642.0 32 35551.5 33 38894.5 34 41390.0 35 44007.5 36 46623.0 37 49036.0 38 50813.0 39 52014.5 40 52888.5 41 53351.5 42 54035.0 43 55153.0 44 55632.0 45 56001.5 46 56100.5 47 55777.0 48 54879.5 49 53239.0 50 51262.0 51 49008.0 52 46548.0 53 43850.5 54 40808.5 55 37037.0 56 32898.0 57 28581.5 58 24570.0 59 20762.5 60 17098.5 61 13830.0 62 10999.5 63 8623.5 64 6581.0 65 4847.5 66 3556.0 67 2657.5 68 1953.0 69 1463.5 70 1069.5 71 797.5 72 590.0 73 419.0 74 302.5 75 216.5 76 153.5 77 102.0 78 63.5 79 39.0 80 24.5 81 15.5 82 9.0 83 3.0 84 2.5 85 2.5 86 1.5 87 2.0 88 1.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.744763123798613 2 21.811664902579896 3 17.543787020647645 4 22.84964162558545 5 22.74016008807753 6 16.52214581575773 7 15.348282197194319 8 12.304057697945082 9 3.1059861841796104 10-11 6.653031357481555 12-13 3.653533730607167 14-15 7.604295569941752 16-17 11.754887788416998 18-19 14.88350314067119 20-21 15.289933069130571 22-23 13.232776657663484 24-25 15.805033353547604 26-27 13.270454642084323 28-29 13.28214684511915 30-31 14.194558258499503 32-33 12.62620866050989 34-35 19.542342558052628 36-37 24.317236880676873 38-39 23.348042758778103 40-41 18.47345702080429 42-43 18.438604185920575 44-45 23.78328361816062 46-47 13.099630996309964 48-49 21.850042181440614 50-51 21.575639043230943 52-53 11.544120444236583 54-55 13.181196699777791 56-57 12.682180187478043 58-59 13.72611489911139 60-61 13.125337059920023 62-63 13.092078616359238 64-65 14.170027009548447 66-67 12.474909315497037 68-69 13.53173901660181 70-71 12.108590916109282 72-73 11.460316998561131 74-75 11.539029580714242 76-77 11.585322872634427 78-79 12.014298053835603 80-81 11.986242360907537 82-83 10.462367889294418 84-85 10.374312733424485 86-87 10.507822027886743 88-89 11.9556692079959 90-91 9.387048843199166 92-93 11.45245692905686 94-95 11.542693883579224 96-97 11.08065606126043 98-99 10.944237701928262 100-101 11.044600439940117 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1787516.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.43513614156524 #Duplication Level Percentage of deduplicated Percentage of total 1 98.84654401732715 96.3112647345149 2 0.8633510897595537 1.6824146193738165 3 0.14508588228294395 0.42409388077373256 4 0.057902386331531615 0.22566907580537157 5 0.030084044643071583 0.1465621492743303 6 0.013276673768480062 0.07761687096834018 7 0.009287612534027097 0.06334578541781266 8 0.00680339206877636 0.053031154595653586 9 0.004658720809491827 0.040853078668654126 >10 0.02054942808832354 0.34230143131838575 >50 0.0014179466727411527 0.09516926901119309 >100 8.080252364327539E-4 0.12342727116195781 >500 5.769511936981568E-5 0.054641289198077966 >1k 1.73085358109447E-4 0.3596093899178032 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3339 0.18679553078126296 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.1188711038110987E-4 0.0 9 0.0 0.0 0.0 1.678306655716648E-4 0.0 10-11 0.0 0.0 0.0 1.678306655716648E-4 5.5943555190554936E-5 12-13 0.0 0.0 0.0 1.678306655716648E-4 5.5943555190554936E-5 14-15 0.0 0.0 0.0 1.678306655716648E-4 5.5943555190554936E-5 16-17 0.0 0.0 0.0 1.678306655716648E-4 1.678306655716648E-4 18-19 0.0 0.0 0.0 1.678306655716648E-4 1.678306655716648E-4 20-21 2.7971777595277468E-5 0.0 0.0 1.958024431669423E-4 2.517459983574972E-4 22-23 5.5943555190554936E-5 0.0 0.0 3.0768955354805216E-4 2.797177759527747E-4 24-25 5.5943555190554936E-5 0.0 0.0 4.475484415244395E-4 2.797177759527747E-4 26-27 5.5943555190554936E-5 0.0 0.0 6.713226622866592E-4 2.797177759527747E-4 28-29 5.5943555190554936E-5 0.0 0.0 0.0015664195453355383 2.797177759527747E-4 30-31 5.5943555190554936E-5 0.0 0.0 0.0059859604053893786 2.797177759527747E-4 32-33 8.39153327858324E-5 0.0 0.0 0.016839010112357035 3.6363310873860713E-4 34-35 1.1188711038110987E-4 0.0 0.0 0.031076644908353268 3.916048863338846E-4 36-37 1.1188711038110987E-4 0.0 0.0 0.052419111213549974 3.916048863338846E-4 38-39 1.1188711038110987E-4 0.0 0.0 0.0822929696853063 3.916048863338846E-4 40-41 1.1188711038110987E-4 0.0 0.0 0.11440457036468485 3.916048863338846E-4 42-43 1.1188711038110987E-4 0.0 0.0 0.15040424812980696 3.916048863338846E-4 44-45 1.1188711038110987E-4 0.0 0.0 0.19566258427896588 4.1957666392916204E-4 46-47 1.1188711038110987E-4 0.0 0.0 0.2478019777165631 4.475484415244395E-4 48-49 1.1188711038110987E-4 0.0 0.0 0.30522803711966773 4.475484415244395E-4 50-51 1.1188711038110987E-4 0.0 0.0 0.37126940402211783 4.475484415244395E-4 52-53 1.1188711038110987E-4 0.0 0.0 0.45812177345545435 4.7552021911971695E-4 54-55 1.1188711038110987E-4 0.0 0.0 0.5549880392679003 5.034919967149944E-4 56-57 1.1188711038110987E-4 0.0 0.0 0.6645814638861973 5.034919967149944E-4 58-59 1.1188711038110987E-4 0.0 0.0 0.7822587322295297 5.034919967149944E-4 60-61 1.1188711038110987E-4 0.0 0.0 0.9042436543225347 5.31463774310272E-4 62-63 1.1188711038110987E-4 0.0 0.0 1.0302285406116645 5.594355519055494E-4 64-65 1.1188711038110987E-4 0.0 0.0 1.1642413270706387 5.594355519055494E-4 66-67 1.1188711038110987E-4 0.0 0.0 1.3110092441130599 5.594355519055494E-4 68-69 1.1188711038110987E-4 0.0 0.0 1.4686861544176386 5.594355519055494E-4 70-71 1.1188711038110987E-4 5.5943555190554936E-5 0.0 1.6380272959794486 5.874073295008269E-4 72-73 1.1188711038110987E-4 5.5943555190554936E-5 0.0 1.8126830752843612 6.433508846913818E-4 74-75 1.1188711038110987E-4 5.5943555190554936E-5 0.0 1.9934087303274488 6.713226622866592E-4 76-77 1.1188711038110987E-4 5.5943555190554936E-5 0.0 2.196064259005234 6.713226622866592E-4 78-79 1.1188711038110987E-4 1.1188711038110987E-4 0.0 2.41192246670799 6.992944398819367E-4 80-81 1.1188711038110987E-4 1.1188711038110987E-4 0.0 2.64375255941765 7.272662174772141E-4 82-83 1.1188711038110987E-4 1.1188711038110987E-4 0.0 2.8776525636693604 7.272662174772141E-4 84-85 1.1188711038110987E-4 1.1188711038110987E-4 0.0 3.1214545771897986 7.272662174772141E-4 86-87 1.1188711038110987E-4 1.1188711038110987E-4 0.0 3.381564137048284 7.272662174772141E-4 88-89 1.1188711038110987E-4 1.1188711038110987E-4 0.0 3.650680609292448 7.272662174772141E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1600 0.0 61.180603 1 GTATCAA 3055 0.0 45.420666 1 ATCAACG 4005 0.0 33.872875 3 TCAACGC 4040 0.0 33.56168 4 TATCAAC 4100 0.0 33.21729 2 CAACGCA 4105 0.0 32.882904 5 AACGCAG 4470 0.0 30.170855 6 TACATGG 3585 0.0 25.466763 2 CGCAGAG 5450 0.0 25.378317 8 GTACATG 3560 0.0 25.370852 1 ACGCAGA 5340 0.0 25.267366 7 ACATGGG 3675 0.0 24.015024 3 GCAGAGT 5710 0.0 23.019087 9 GAGTACA 2565 0.0 21.04885 1 AGAGTAC 5210 0.0 20.226995 10-11 CATGGGG 2690 0.0 19.862154 4 GTGGTAT 720 0.0 19.622644 1 AGTACAT 2745 0.0 19.2944 2 GAGTACT 4005 0.0 18.488768 12-13 CAGAGTA 5645 0.0 18.394358 10-11 >>END_MODULE