FastQCFastQC Report
Sat 18 Jun 2016
SRR1930945_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930945_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1787516
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49580.2773681466347714No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39470.22080921233712034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32550.1820962721452563No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22700.056.272091
GTATCAA42800.041.4948771
ATCAACG55700.031.1992323
CAACGCA56300.031.1023315
TCAACGC56550.030.8964734
TATCAAC57050.030.5450332
AACGCAG59250.029.6322126
ACGCAGA69300.025.2664267
GTGGTAT10500.024.4214321
CGCAGAG71600.024.3885218
GTACATG44250.023.2869781
TACATGG44800.022.575212
CTAACGC1703.16168E-622.3439063
ACATGGG45300.021.801343
GCAGAGT79400.021.454819
AGAGTAC73800.020.0286810-11
AGTACTT59900.019.0123212-13
GAGTACT55600.018.90382212-13
CAGAGTA77450.018.56401410-11
TGGTATC11950.018.277642
Produced by FastQC (version 0.11.5)