##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930945_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1787516 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.24849399949427 31.0 31.0 33.0 30.0 34.0 2 31.37373036101495 31.0 31.0 34.0 30.0 34.0 3 31.45071037126381 31.0 31.0 34.0 28.0 34.0 4 35.32008161045831 37.0 35.0 37.0 33.0 37.0 5 35.09914037133094 37.0 35.0 37.0 32.0 37.0 6 35.13935987146409 37.0 35.0 37.0 32.0 37.0 7 35.01839088433334 36.0 35.0 37.0 32.0 37.0 8 34.997779600294486 36.0 35.0 37.0 32.0 37.0 9 36.500764188963906 38.0 35.0 39.0 32.0 39.0 10-11 36.43425177732675 38.0 35.0 39.0 32.0 39.0 12-13 36.32730672061117 38.0 35.0 39.0 32.0 39.0 14-15 37.33383673209079 39.0 36.0 41.0 32.0 41.0 16-17 37.3083426945549 39.0 36.0 41.0 32.0 41.0 18-19 37.31944105675138 39.0 36.0 40.5 32.0 41.0 20-21 37.29753356053876 39.0 36.0 40.0 31.5 41.0 22-23 37.16932967313299 39.0 36.0 40.0 31.0 41.0 24-25 37.051553664414755 39.0 36.0 40.0 31.0 41.0 26-27 36.77979190116341 39.0 35.5 40.0 30.5 41.0 28-29 36.59246350801895 38.0 35.0 40.0 30.0 41.0 30-31 36.34367832231991 38.0 35.0 40.0 30.0 41.0 32-33 36.09528641981386 38.0 35.0 40.0 30.0 41.0 34-35 36.2541456971574 38.0 35.0 40.0 30.0 41.0 36-37 36.29323877380678 38.0 35.0 40.0 30.0 41.0 38-39 36.24476368323417 38.0 35.0 40.0 30.0 41.0 40-41 36.1441939540681 38.0 35.0 40.0 30.0 41.0 42-43 35.99634828443493 38.0 35.0 40.0 29.5 41.0 44-45 35.80062528111637 38.0 35.0 40.0 28.5 41.0 46-47 35.55690466546873 38.0 34.0 40.0 27.5 41.0 48-49 35.34274965930375 38.0 34.0 40.0 27.0 41.0 50-51 35.08328400976551 38.0 34.0 40.0 26.5 41.0 52-53 34.87284001933409 38.0 33.5 40.0 26.0 41.0 54-55 34.708532958586105 38.0 33.0 40.0 26.0 41.0 56-57 34.45891309504363 37.5 33.0 40.0 25.5 41.0 58-59 34.21736420820849 37.0 33.0 40.0 25.0 41.0 60-61 33.909539271256875 37.0 32.5 40.0 24.5 41.0 62-63 33.60775567883029 36.5 32.0 39.5 23.5 41.0 64-65 33.2983612454378 36.0 32.0 39.0 23.0 41.0 66-67 32.959182183544094 35.5 31.0 39.0 22.5 40.0 68-69 32.592878049762916 35.0 31.0 38.5 22.0 40.0 70-71 32.197954591735126 35.0 31.0 38.0 21.5 40.0 72-73 31.78078433983248 35.0 30.5 37.5 20.5 39.5 74-75 31.252341237784727 34.0 30.0 37.0 19.0 39.0 76-77 29.877144316470453 33.0 28.5 35.5 18.0 38.0 78-79 30.641994812913563 34.0 29.5 36.0 18.0 38.5 80-81 30.641511740314492 34.0 30.0 36.0 18.0 37.5 82-83 30.36056712219639 34.0 30.0 35.0 18.0 37.0 84-85 30.035652548005164 34.0 30.0 35.0 16.0 37.0 86-87 29.699854994304946 34.0 29.0 35.0 12.0 36.0 88-89 29.35788966364497 34.0 29.0 35.0 7.5 36.0 90-91 29.02495138505054 34.0 29.0 35.0 4.5 35.5 92-93 28.679668881285536 34.0 29.0 35.0 2.0 35.0 94-95 28.272102739220237 33.0 29.0 35.0 2.0 35.0 96-97 27.819436021831415 33.0 27.0 35.0 2.0 35.0 98-99 27.33801319820354 33.0 27.0 35.0 2.0 35.0 100-101 26.14109020562613 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.47605004840194454 1101 2 0.5369582334086083 1101 3 0.49095023942066973 1101 4 0.32685559969921485 1101 5 0.550023263676465 1101 6 0.399392356993026 1101 7 0.4571800598634539 1101 8 0.5113663827529393 1101 9 0.5803490888222029 1101 10-11 0.6245246057046785 1101 12-13 0.6637297214167219 1101 14-15 0.6795950541632507 1101 16-17 0.7619902594519914 1101 18-19 0.6802788879601422 1101 20-21 0.6328652891768627 1101 22-23 0.7343497999569948 1101 24-25 0.7232101702280147 1101 26-27 0.7977704088824211 1101 28-29 0.6371747407279784 1101 30-31 0.5428296211095684 1101 32-33 0.6348229615527288 1101 34-35 0.5912116593149648 1101 36-37 0.7534665854894058 1101 38-39 0.6951250163206026 1101 40-41 0.8644179925810249 1101 42-43 0.7128641707963226 1101 44-45 0.7057239638517956 1101 46-47 0.6530976167547777 1101 48-49 0.7615879812065387 1101 50-51 0.6934726786085292 1101 52-53 0.7829832685438092 1101 54-55 0.6759841897605767 1101 56-57 0.9150796542076307 1101 58-59 0.8257897207584151 1101 60-61 0.6478614720542666 1101 62-63 0.5026364122446267 1101 64-65 0.7008968769198347 1101 66-67 0.7444667093011503 1101 68-69 0.6755765577393973 1101 70-71 0.548995370991868 1101 72-73 0.6135298268742346 1101 74-75 0.6711071374164383 1101 76-77 0.8147824956239766 1101 78-79 0.7069658015813474 1101 80-81 0.7504376360793046 1101 82-83 0.758179535858531 1101 84-85 0.9182485863631946 1101 86-87 0.8681566351714949 1101 88-89 0.7595899819108247 1101 90-91 0.9576735089552031 1101 92-93 0.9685352404706187 1101 94-95 0.9207946697125458 1101 96-97 0.8713823341879028 1101 98-99 1.0694069748603425 1101 100-101 1.0533590428058979 1102 1 0.4779607957804224 1102 2 0.43889430394511564 1102 3 0.5642694148065601 1102 4 0.17820290681157047 1102 5 0.4132523116408251 1102 6 0.4588010612530695 1102 7 0.4600749572471159 1102 8 0.5575005724694364 1102 9 0.7331685922580959 1102 10-11 0.6867692159007035 1102 12-13 0.5929917815707313 1102 14-15 0.8267801549379072 1102 16-17 0.7180819177735174 1102 18-19 0.7522570870231817 1102 20-21 0.7515057233539153 1102 22-23 0.7831299719242608 1102 24-25 0.7647847526836671 1102 26-27 0.8640681631787217 1102 28-29 0.8557150333255592 1102 30-31 0.8330157463210668 1102 32-33 0.9663894290872861 1102 34-35 0.8264954513427725 1102 36-37 0.774330853362379 1102 38-39 0.7834769391540277 1102 40-41 0.6949182976099735 1102 42-43 0.835508829336554 1102 44-45 0.8215615848562052 1102 46-47 0.8660711310335856 1102 48-49 0.7700136865140408 1102 50-51 0.7843770606659319 1102 52-53 0.5135563776520868 1102 54-55 0.5936868038011198 1102 56-57 0.6289078250698878 1102 58-59 0.7825907152678937 1102 60-61 0.7222842191564922 1102 62-63 0.42111167036818387 1102 64-65 0.6168556462143542 1102 66-67 0.708510702532962 1102 68-69 0.6079248777780819 1102 70-71 0.6593410320989506 1102 72-73 0.685853341728567 1102 74-75 0.7769349169207906 1102 76-77 0.9511937437103519 1102 78-79 0.9047943407804482 1102 80-81 0.7789966908348802 1102 82-83 0.9228145946197692 1102 84-85 0.9335446371015976 1102 86-87 0.8585295186734605 1102 88-89 0.8330213849288839 1102 90-91 1.008466584222532 1102 92-93 0.9725725575971289 1102 94-95 0.9724150640631777 1102 96-97 0.9530639069862268 1102 98-99 1.1591933970434312 1102 100-101 1.4638632614138167 1103 1 0.472529165764076 1103 2 0.5273296780947732 1103 3 0.45686288630368566 1103 4 0.23594693465526717 1103 5 0.3307363595235415 1103 6 0.43062777643526573 1103 7 0.5025524133458319 1103 8 0.45208476273469245 1103 9 0.4435256032795607 1103 10-11 0.5353229784910098 1103 12-13 0.5626959423047921 1103 14-15 0.5910052248108215 1103 16-17 0.6390662029604641 1103 18-19 0.6440727280082541 1103 20-21 0.6121751849369943 1103 22-23 0.6341226945947298 1103 24-25 0.6575562023488004 1103 26-27 0.6506789315170636 1103 28-29 0.6607893886490857 1103 30-31 0.5724952590563959 1103 32-33 0.7838233794116078 1103 34-35 0.8236873513585934 1103 36-37 0.8483631794711144 1103 38-39 0.6753980486831139 1103 40-41 0.8054427399450503 1103 42-43 0.8051866229346203 1103 44-45 0.8710592909153689 1103 46-47 0.8863870093228883 1103 48-49 0.7324273341436296 1103 50-51 0.6601746665299828 1103 52-53 0.6345815318998334 1103 54-55 0.6819639751347708 1103 56-57 0.6912661017138788 1103 58-59 0.8451203782958956 1103 60-61 0.732283955485137 1103 62-63 0.6081008071081513 1103 64-65 0.5629744037949038 1103 66-67 0.30716334188328176 1103 68-69 0.42257050347021874 1103 70-71 0.5047636972890572 1103 72-73 0.36927632332429994 1103 74-75 0.5808557100442435 1103 76-77 0.6409316543784946 1103 78-79 0.7895304057490691 1103 80-81 0.6505557795866608 1103 82-83 0.5448021493315771 1103 84-85 0.6607172891081348 1103 86-87 0.7713136245638808 1103 88-89 0.8632513063548188 1103 90-91 0.9139140345204808 1103 92-93 0.7270549707174148 1103 94-95 0.7855709465712195 1103 96-97 0.9279623935126224 1103 98-99 0.9534572266062611 1103 100-101 1.1134506157353918 1104 1 0.5175349182441025 1104 2 0.5912719486821025 1104 3 0.4810977885115264 1104 4 0.23443836695997078 1104 5 0.37081615835725046 1104 6 0.39476048013677456 1104 7 0.4271473615735104 1104 8 0.4179216722978083 1104 9 0.5495704636495446 1104 10-11 0.48670958643032947 1104 12-13 0.5961671197773271 1104 14-15 0.69409217969379 1104 16-17 0.7480900582528349 1104 18-19 0.7846894548919963 1104 20-21 0.6073250635156953 1104 22-23 0.6161697518556863 1104 24-25 0.5953015353016298 1104 26-27 0.7816205433960661 1104 28-29 0.6534484702984074 1104 30-31 0.6398769024528903 1104 32-33 0.7289226433454346 1104 34-35 0.7963159565302576 1104 36-37 0.8198417373486038 1104 38-39 0.7321910006835566 1104 40-41 0.76608087933635 1104 42-43 0.7533873800004756 1104 44-45 0.7307956998960634 1104 46-47 0.8113332985694228 1104 48-49 0.8121126438711528 1104 50-51 0.7999487134221113 1104 52-53 0.6905098170505326 1104 54-55 0.7244241878957638 1104 56-57 0.7629069136302178 1104 58-59 0.9027353695639704 1104 60-61 0.8091571909275928 1104 62-63 0.6917325335162516 1104 64-65 0.7544655794367046 1104 66-67 0.7481739476257019 1104 68-69 0.6392496443135443 1104 70-71 0.8293135051508429 1104 72-73 0.8433973425812056 1104 74-75 1.1533356122610563 1104 76-77 1.131572432192769 1104 78-79 1.2203444938508596 1104 80-81 1.008526509245229 1104 82-83 1.1085626918057017 1104 84-85 1.3307214710982933 1104 86-87 1.2012267001932884 1104 88-89 1.1783081760364666 1104 90-91 1.091101637163213 1104 92-93 1.317145448882222 1104 94-95 1.3108508976078355 1104 96-97 1.441210597320925 1104 98-99 1.595705468106324 1104 100-101 1.9293061916923584 1105 1 0.1364607688438042 1105 2 0.270027405133753 1105 3 0.27757928879215044 1105 4 0.1423490404269998 1105 5 0.12434848517143138 1105 6 0.18983155840513177 1105 7 0.21579409155310714 1105 8 0.22116503033321777 1105 9 0.4372416541682469 1105 10-11 0.23568611887499458 1105 12-13 0.2938446929296603 1105 14-15 0.4421864326397724 1105 16-17 0.36739527882708956 1105 18-19 0.36697831748800525 1105 20-21 0.27422859940138267 1105 22-23 0.5544656120118319 1105 24-25 0.4780597315016877 1105 26-27 0.4349500436486906 1105 28-29 0.4376066452619156 1105 30-31 0.5464786692872323 1105 32-33 0.4617502083882101 1105 34-35 0.6305260268559181 1105 36-37 0.7703815774677167 1105 38-39 0.558073746919618 1105 40-41 0.711328209306842 1105 42-43 0.5529308233321402 1105 44-45 0.48997117331362006 1105 46-47 0.3913303347870851 1105 48-49 0.5566340221933004 1105 50-51 0.5735080118224829 1105 52-53 0.5849243416843493 1105 54-55 0.5587016434302754 1105 56-57 0.5597737639580416 1105 58-59 0.5762721187147974 1105 60-61 0.5674225264592607 1105 62-63 0.4127013227878251 1105 64-65 0.5136308429741305 1105 66-67 0.484038660296342 1105 68-69 0.5476168452778225 1105 70-71 0.5309770155059894 1105 72-73 0.48101748369924735 1105 74-75 0.7540963358440145 1105 76-77 0.6734202513040302 1105 78-79 0.689381573801807 1105 80-81 0.7244371780448802 1105 82-83 0.5907699337028447 1105 84-85 0.6712425600432681 1105 86-87 0.7566313439968226 1105 88-89 0.7058278869393355 1105 90-91 0.5240990070980942 1105 92-93 0.7394061088722133 1105 94-95 0.5281493808280402 1105 96-97 0.34793517442768973 1105 98-99 0.8552075118326137 1105 100-101 0.9614028460021515 1106 1 0.09928426434059645 1106 2 0.0707777142435404 1106 3 0.19361628737553715 1106 4 0.08959374051731572 1106 5 0.11132983948894548 1106 6 0.1395986844385959 1106 7 0.07909774884550558 1106 8 0.14140907504321376 1106 9 0.16155795286852026 1106 10-11 0.13087228291826136 1106 12-13 0.1559293653633702 1106 14-15 0.24728680041848605 1106 16-17 0.29677044914714656 1106 18-19 0.2764679879285552 1106 20-21 0.18999364893777937 1106 22-23 0.3528764836676501 1106 24-25 0.3193203138667755 1106 26-27 0.2877403084358505 1106 28-29 0.23894533274459917 1106 30-31 0.19632822459704613 1106 32-33 0.27489531319275073 1106 34-35 0.37562897336471934 1106 36-37 0.48630968382400397 1106 38-39 0.36974818731266623 1106 40-41 0.3252934403678154 1106 42-43 0.4564888515157506 1106 44-45 0.47872554533090295 1106 46-47 0.4786180962917115 1106 48-49 0.49990759054767153 1106 50-51 0.40257227062338785 1106 52-53 0.2405785733916943 1106 54-55 0.4371373226948094 1106 56-57 0.5822115309804801 1106 58-59 0.5782483943979386 1106 60-61 0.3798536946352513 1106 62-63 0.34303141150064675 1106 64-65 0.2772823473084216 1106 66-67 0.34516925889954564 1106 68-69 0.4076728791684161 1106 70-71 0.22697514170386768 1106 72-73 0.18930849507944458 1106 74-75 0.41834792981451585 1106 76-77 0.5378001624335589 1106 78-79 0.5894774597701371 1106 80-81 0.44208199795042447 1106 82-83 0.5839033610265751 1106 84-85 0.46847892711930683 1106 86-87 0.6797615394623122 1106 88-89 0.6359493687744902 1106 90-91 0.6138053133881627 1106 92-93 0.7310097524263988 1106 94-95 0.6663070977623065 1106 96-97 0.6377759913333492 1106 98-99 0.5709669872581031 1106 100-101 0.7263245719721034 1107 1 0.18180649250921022 1107 2 0.2971297621138511 1107 3 0.29031632898825066 1107 4 0.079920002575669 1107 5 0.2178229376770915 1107 6 0.25716027693380283 1107 7 0.2743733803008155 1107 8 0.2949892022406644 1107 9 0.39777773292179575 1107 10-11 0.4205159666335163 1107 12-13 0.31231334517281795 1107 14-15 0.36340317640043196 1107 16-17 0.48184636664250036 1107 18-19 0.3375290958440118 1107 20-21 0.43374254300825754 1107 22-23 0.49448781552874976 1107 24-25 0.42092770631902 1107 26-27 0.43441443003695923 1107 28-29 0.491451294617832 1107 30-31 0.5418031533440768 1107 32-33 0.58099603976477 1107 34-35 0.5312592977220092 1107 36-37 0.6298487827275139 1107 38-39 0.706839832140318 1107 40-41 0.46247537233400493 1107 42-43 0.6479585973427007 1107 44-45 0.7359675911611703 1107 46-47 0.782658073715865 1107 48-49 0.6475423489044374 1107 50-51 0.39725975050021134 1107 52-53 0.38907399518171104 1107 54-55 0.5971384698853015 1107 56-57 0.7183313647677281 1107 58-59 0.6903038783743369 1107 60-61 0.5467609827039794 1107 62-63 0.2943786298072055 1107 64-65 0.3961517099088141 1107 66-67 0.4738771327930067 1107 68-69 0.39119581459079455 1107 70-71 0.3775970596440317 1107 72-73 0.3949334622735847 1107 74-75 0.7507789779840728 1107 76-77 0.763468766156624 1107 78-79 0.8098136569310554 1107 80-81 0.9313902637284883 1107 82-83 0.9101370101800086 1107 84-85 1.014439328484464 1107 86-87 0.8321630907668904 1107 88-89 0.9496908169293263 1107 90-91 0.8795011250064988 1107 92-93 1.0815628930497674 1107 94-95 0.9681918992739966 1107 96-97 0.9050391790185834 1107 98-99 0.9584100132868834 1107 100-101 1.0798865172824996 1108 1 0.26341789661863046 1108 2 0.3178362960774308 1108 3 0.33001202708043564 1108 4 0.22707165558903597 1108 5 0.34133291555438205 1108 6 0.2827978591202438 1108 7 0.29002866396941585 1108 8 0.37179860265062814 1108 9 0.35633841396739996 1108 10-11 0.28427216809329536 1108 12-13 0.367024078195449 1108 14-15 0.5078328742151825 1108 16-17 0.5551924275791436 1108 18-19 0.4714691594986036 1108 20-21 0.4408417879181883 1108 22-23 0.650539395757697 1108 24-25 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-0.16334651496990915 2316 96-97 0.03858964582688529 2316 98-99 -0.004176831342583398 2316 100-101 -0.34254576628566014 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 16.0 4 24.0 5 54.0 6 120.0 7 312.0 8 811.0 9 1472.0 10 2418.0 11 3770.0 12 5189.0 13 6662.0 14 7919.0 15 9726.0 16 11555.0 17 12718.0 18 11542.0 19 10071.0 20 10326.0 21 10757.0 22 12066.0 23 13944.0 24 16918.0 25 21130.0 26 27123.0 27 34227.0 28 43209.0 29 54100.0 30 65833.0 31 78845.0 32 93949.0 33 113320.0 34 139799.0 35 173210.0 36 222995.0 37 285874.0 38 243727.0 39 41778.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.68727268657886 22.233238957463712 14.806678678140983 23.272809677816447 2 15.490860184373975 22.424856807503858 39.33041074064004 22.753872267482127 3 17.87172264476588 26.71021088642791 31.52689158608189 23.89117488272432 4 12.75877623019444 18.209138651229633 38.886948813345334 30.14513630523059 5 12.577224424260901 38.548519288273745 35.955938091788184 12.918318195677166 6 29.872739600652526 39.240599804421336 17.716037227079365 13.170623367846776 7 25.59977085519794 33.569881332530734 23.454167682974585 17.376180129296745 8 24.83244905220429 36.47911403310516 21.061126166143406 17.627310748547146 9 25.43540869004809 15.87364812398882 21.244285365837285 37.44665782012581 10-11 23.81704555371812 27.0256601898948 29.512071500339015 19.64522275604806 12-13 24.4621866321756 24.603667883252513 29.618112509202714 21.31603297536917 14-15 21.74462871640238 26.31670223746374 26.965396094578225 24.97327295155565 16-17 21.249376229359626 29.282199432061027 27.88951259736976 21.578911741209588 18-19 21.162272113927934 28.202320986217742 29.977130274638103 20.65827662521622 20-21 21.801539119090403 27.357489387507584 29.92149440900109 20.919477084400924 22-23 21.597161422421628 27.215715672316037 29.7724774337558 21.41464547150653 24-25 21.49490700013007 27.394031616738996 29.81576249543707 21.295298887693864 26-27 21.513218274895472 27.571366991921813 29.68485878212679 21.230555951055923 28-29 21.45090891595814 27.733768798454168 29.63115253922487 21.184169746362826 30-31 21.74603996055696 27.5216202418334 29.45046891063074 21.2818708869789 32-33 21.638426757114953 27.603794642544884 29.36083265695763 21.396945943382526 34-35 21.779826333433206 27.582541135935674 29.47525437460537 21.16237815602575 36-37 21.74713650670041 27.485283932549446 29.45641459422143 21.311164966528708 38-39 21.691606025725836 27.557807611457886 29.447343430288907 21.30324293252737 40-41 21.683575994069848 27.54256702890949 29.508328787815213 21.265528189205444 42-43 21.68214301499817 27.58873327063347 29.188586908782888 21.54053680558547 44-45 21.601917032292572 27.73122831281688 29.193703961068536 21.473150693822014 46-47 21.763418240106528 27.68211642639043 28.933772157422784 21.620693176080263 48-49 21.75871025676532 27.799024523976374 28.733685534168934 21.708579685089372 50-51 21.752659002744696 27.885665054079727 28.627038377252294 21.734637565923283 52-53 21.816654762997576 27.91663214975786 28.640199847304658 21.626513239939907 54-55 21.613276133253727 27.809528658884936 28.734087334382664 21.843107873478676 56-57 21.69955444352158 27.9249802287285 28.582922265263182 21.79254306248673 58-59 21.634672819512513 27.93708720718936 28.663946679066367 21.764293294231763 60-61 21.62622673695317 28.061052422407535 28.65215316562156 21.660567675017735 62-63 21.654793962071 28.002045160985027 28.56326040773857 21.7799004692054 64-65 21.439684183349733 27.956454802410178 28.531249842489302 22.072611171750786 66-67 21.413713787099198 28.010570607840755 28.460552321820515 22.115163283239532 68-69 21.499690568933122 27.922891458302413 28.522935702744554 22.05448227001991 70-71 21.48634240255893 27.7849425394205 28.48196216035203 22.246752897668536 72-73 21.51581994127181 27.905239909416384 28.347828764496597 22.231111384815208 74-75 21.481389464049766 28.040139623885914 28.20858449107049 22.269886420993835 76-77 21.433328825762953 27.91048301889757 28.29717083560816 22.35901731973132 78-79 21.35665866987732 27.991382937298308 28.213019698061526 22.43893869476285 80-81 21.432821175254958 28.0033051720516 28.13832603776836 22.42554761492508 82-83 21.49130413378118 27.83975080502776 28.262907856489118 22.406037204701946 84-85 21.480087450965474 27.904980990379947 28.02419670649102 22.59073485216356 86-87 21.325962956415495 27.990574629821495 28.111804313919425 22.57165809984358 88-89 21.306670217223594 27.781028725385493 28.027824133307 22.884476924083906 90-91 21.227400770394905 27.75514086142123 28.115413772557268 22.9020445956266 92-93 21.3169428657598 27.632237881306665 28.16632040363098 22.88449884930256 94-95 21.087663186821025 27.611499630625964 28.20188270269348 23.098954479859536 96-97 21.104034872974566 27.466691207239542 28.163244412917145 23.266029506868747 98-99 21.0143853257817 27.509320196294745 28.05356707296606 23.422727404957495 100-101 20.965294856101988 27.437180981876523 28.102517683757792 23.49500647826369 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 667.0 1 481.5 2 388.5 3 506.5 4 440.0 5 409.0 6 730.5 7 1516.5 8 2271.5 9 2410.5 10 1921.5 11 1476.5 12 1390.5 13 1398.5 14 1589.5 15 1790.0 16 1903.0 17 2076.0 18 2289.0 19 2571.5 20 2968.5 21 3486.5 22 4244.5 23 5277.5 24 6735.0 25 8588.5 26 10951.0 27 13852.5 28 17502.0 29 22088.5 30 26866.0 31 31778.0 32 37515.0 33 41592.0 34 43717.5 35 46605.5 36 50459.5 37 54482.5 38 56798.5 39 57606.5 40 58841.0 41 60034.0 42 60880.0 43 62580.5 44 64282.5 45 65817.5 46 67198.5 47 67381.0 48 67446.5 49 66568.5 50 63795.5 51 61100.5 52 59219.5 53 57432.0 54 54453.0 55 49322.0 56 43401.0 57 37406.5 58 31969.0 59 27126.0 60 22014.0 61 17513.5 62 13930.0 63 10476.0 64 7424.0 65 5292.0 66 3779.5 67 2790.0 68 1993.0 69 1418.0 70 1068.0 71 775.5 72 531.0 73 383.0 74 283.5 75 205.5 76 147.5 77 95.5 78 58.5 79 31.5 80 18.5 81 8.5 82 6.0 83 5.0 84 2.5 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01879703454402646 2 0.003468500421814406 3 0.003412556866623851 4 0.03602764954271738 5 0.001734250210907203 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 5.5943555190554936E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.5943555190554936E-5 24-25 7.552379950724916E-4 26-27 0.0026573188715513594 28-29 1.1188711038110987E-4 30-31 0.007188746841986309 32-33 0.023356434292056687 34-35 0.013454425023328461 36-37 7.832097726677692E-4 38-39 7.552379950724916E-4 40-41 0.0022936857628127524 42-43 0.009230686606441565 44-45 0.009510404382394338 46-47 0.009146771273655733 48-49 0.01026564237746683 50-51 0.19745277804506367 52-53 0.05325826454140831 54-55 0.018964865209598124 56-57 0.011216682815706265 58-59 0.02100680497405338 60-61 0.05697851096158021 62-63 0.1027962826626447 64-65 0.10757945663143714 66-67 0.11183116682591933 68-69 0.11082418283248933 70-71 0.09902009268728224 72-73 0.07390143640672307 74-75 0.2483893850460639 76-77 0.29812320561046723 78-79 0.0073845492851532515 80-81 8.111815502630466E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.678306655716648E-4 90-91 0.006769170178057148 92-93 0.018964865209598124 94-95 0.0018181655436930355 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1787516.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.8474846277003 #Duplication Level Percentage of deduplicated Percentage of total 1 81.71476992849205 53.807120567224466 2 10.863248952464073 14.306352368085188 3 3.209059298615966 6.339254485049805 4 1.3749696769574677 3.621531786680436 5 0.7891991160681637 2.5983388331746533 6 0.46883151595099715 1.8522825623738823 7 0.3126122523715196 1.4409311337725084 8 0.22959147965393845 1.209441714173094 9 0.16896816701349848 1.0013515901992833 >10 0.8166527061095545 9.379175440318717 >50 0.0360144374074635 1.596965090379729 >100 0.014806447214739182 1.7307592229145328 >500 0.0010208173445690216 0.4353502276911922 >1k 2.5520433614225523E-4 0.6811449779625441 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4958 0.2773681466347714 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3947 0.22080921233712034 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3255 0.1820962721452563 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 5.5943555190554936E-5 0.0 0.0 0.0 4 0.0 5.5943555190554936E-5 0.0 0.0 0.0 5 0.0 5.5943555190554936E-5 0.0 0.0 0.0 6 0.0 5.5943555190554936E-5 0.0 0.0 0.0 7 0.0 5.5943555190554936E-5 0.0 0.0 0.0 8 0.0 5.5943555190554936E-5 0.0 0.0 0.0 9 0.0 5.5943555190554936E-5 0.0 0.0 5.5943555190554936E-5 10-11 0.0 5.5943555190554936E-5 0.0 0.0 5.5943555190554936E-5 12-13 0.0 5.5943555190554936E-5 0.0 0.0 1.678306655716648E-4 14-15 0.0 5.5943555190554936E-5 0.0 0.0 2.2377422076221975E-4 16-17 0.0 5.5943555190554936E-5 0.0 0.0 2.797177759527747E-4 18-19 0.0 5.5943555190554936E-5 0.0 0.0 2.797177759527747E-4 20-21 0.0 5.5943555190554936E-5 0.0 2.7971777595277468E-5 3.356613311433296E-4 22-23 0.0 5.5943555190554936E-5 0.0 1.678306655716648E-4 4.1957666392916204E-4 24-25 0.0 5.5943555190554936E-5 0.0 5.31463774310272E-4 4.7552021911971695E-4 26-27 0.0 5.5943555190554936E-5 0.0 8.111815502630465E-4 6.713226622866592E-4 28-29 0.0 5.5943555190554936E-5 0.0 0.0029650084250994117 6.713226622866592E-4 30-31 0.0 5.5943555190554936E-5 0.0 0.012671215250660693 6.713226622866592E-4 32-33 0.0 5.5943555190554936E-5 0.0 0.03611156487550321 6.713226622866592E-4 34-35 0.0 5.5943555190554936E-5 0.0 0.06830708088766757 6.713226622866592E-4 36-37 0.0 5.5943555190554936E-5 0.0 0.11860033700397646 6.713226622866592E-4 38-39 0.0 5.5943555190554936E-5 0.0 0.19079549497738763 6.992944398819367E-4 40-41 0.0 8.39153327858324E-5 0.0 0.26707453247970925 7.272662174772141E-4 42-43 0.0 1.1188711038110987E-4 0.0 0.34712975995739337 7.272662174772141E-4 44-45 0.0 1.1188711038110987E-4 0.0 0.4491987764025609 7.832097726677692E-4 46-47 0.0 1.1188711038110987E-4 0.0 0.5651138227573907 8.671251054536015E-4 48-49 0.0 1.1188711038110987E-4 0.0 0.6851686921963216 8.95096883048879E-4 50-51 0.0 1.1188711038110987E-4 0.0 0.8097549896056875 8.95096883048879E-4 52-53 0.0 1.1188711038110987E-4 0.0 0.9363832267795086 8.95096883048879E-4 54-55 0.0 1.1188711038110987E-4 0.0 1.0776127318580646 8.95096883048879E-4 56-57 0.0 1.1188711038110987E-4 0.0 1.2386742272516722 9.230686606441564E-4 58-59 0.0 1.1188711038110987E-4 0.0 1.4094699012484364 9.510404382394339E-4 60-61 0.0 1.1188711038110987E-4 0.0 1.590307443401905 9.510404382394339E-4 62-63 0.0 1.1188711038110987E-4 0.0 1.7750050908635222 0.0010069839934299888 64-65 0.0 1.1188711038110987E-4 0.0 1.9720103204670616 0.0010069839934299888 66-67 0.0 1.1188711038110987E-4 0.0 2.1972111018866407 0.0010069839934299888 68-69 0.0 1.1188711038110987E-4 0.0 2.4403977362999827 0.0010069839934299888 70-71 0.0 1.3985888797638733E-4 0.0 2.700647155046444 0.0010069839934299888 72-73 0.0 2.2377422076221975E-4 0.0 2.9699034861785854 0.0012867017693827636 74-75 0.0 2.2377422076221975E-4 0.0 3.2589638358481827 0.0014545324349544283 76-77 0.0 2.2377422076221975E-4 0.0 3.5862895772681194 0.0014545324349544283 78-79 0.0 2.2377422076221975E-4 0.0 3.933195563004751 0.0014545324349544283 80-81 0.0 2.2377422076221975E-4 0.0 4.306450963236133 0.0014545324349544283 82-83 0.0 2.2377422076221975E-4 0.0 4.690895074505627 0.0015104759901449834 84-85 0.0 2.2377422076221975E-4 0.0 5.100541757388465 0.0015104759901449834 86-87 0.0 2.2377422076221975E-4 0.0 5.542663674059421 0.0015384477677402608 88-89 0.0 2.2377422076221975E-4 0.0 6.0108832592267705 0.0015664195453355383 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2270 0.0 56.27209 1 GTATCAA 4280 0.0 41.494877 1 ATCAACG 5570 0.0 31.199232 3 CAACGCA 5630 0.0 31.102331 5 TCAACGC 5655 0.0 30.896473 4 TATCAAC 5705 0.0 30.545033 2 AACGCAG 5925 0.0 29.632212 6 ACGCAGA 6930 0.0 25.266426 7 GTGGTAT 1050 0.0 24.421432 1 CGCAGAG 7160 0.0 24.388521 8 GTACATG 4425 0.0 23.286978 1 TACATGG 4480 0.0 22.57521 2 CTAACGC 170 3.16168E-6 22.343906 3 ACATGGG 4530 0.0 21.80134 3 GCAGAGT 7940 0.0 21.45481 9 AGAGTAC 7380 0.0 20.02868 10-11 AGTACTT 5990 0.0 19.01232 12-13 GAGTACT 5560 0.0 18.903822 12-13 CAGAGTA 7745 0.0 18.564014 10-11 TGGTATC 1195 0.0 18.27764 2 >>END_MODULE