FastQCFastQC Report
Sat 18 Jun 2016
SRR1930944_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930944_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3582421
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN65700.18339553056438648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26300.052.9434551
GTATCAA50200.043.3144231
ATCAACG63300.034.0112463
TCAACGC66300.033.224664
TATCAAC65550.032.16062
CAACGCA67050.032.1051525
AACGCAG72550.029.9589616
ACGCAGA81250.026.151657
CGCAGAG84350.025.024898
GTACATG64150.025.0086021
TACATGG65800.023.4539532
ACATGGG70400.021.772053
GCAGAGT94250.020.7013449
AGAGTAC81400.019.40381810-11
GTGGTAT15750.019.2189481
GAGTACA49000.018.6355171
GAGTACT58650.018.34379612-13
CAGAGTA88650.018.06646510-11
CATGGGG55700.017.4660514
TATACTG14550.017.2883635
Produced by FastQC (version 0.11.5)